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Citations for Millipore Sigma :
901 - 950 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Cells were blocked at RT with 5% BSA (Sigma) dissolved into PBS ...
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bioRxiv - Microbiology 2021Quote: ... and 2 µL ThermaSTOP RT (Sigma Aldrich, #TSTOPRT-250). Mix 1 was heated to 65°C for 5 min and then snap-chilled on ice for at least 2 min ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and Hoechst 33342 (1μg/mL; 5 min; RT; Sigma).
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bioRxiv - Molecular Biology 2022Quote: RNA was isolated by using RNAZol-RT reagent (Sigma) from exponentially growing MtbH37Rv/Msm mc2155 and its Rho CKD strain ...
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bioRxiv - Developmental Biology 2023Quote: ... overnight at RT and included in paraffin (Sigma-Aldrich) following standard procedures ...
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bioRxiv - Cell Biology 2023Quote: ... at RT and fixed with 4% paraformaldehyde (Sigma Aldrich) in CB for 20 min (RT) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... DNA was purified from cells harvested at the indicated time points using a Mammalian Genomic DNA Miniprep Kit (Sigma). DNA was immobilized on nitrocellulose and analyzed by immunodot blotting with antibodies against cyclobutane pyrimidine dimers (CPDs ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was washed with 0.1% TBST three times for 15 minutes each and developed using ECL kit (Millipore). The blots were imaged on an Amersham Imager 600 and band intensity was quantified using Image J.
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bioRxiv - Neuroscience 2021Quote: Genomic DNA was extracted from tail tissues and amplified with specific PCR primers using REDExtract-N-Amp™ Tissue PCR kit (Cat. No. XNAT-100RXN, Sigma-Aldrich, Saint Louis, MO) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue digestion for DNA extraction and subsequent polymerase chain reaction (PCR) was performed with the prepared sample buffer from REDextract-N-Amp PCR KIT (Sigma-Aldrich, St. Louis, United States) or DreamTaq™ Hot Start Green DNA Polymerase (Thermo Fischer Scientific ...
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bioRxiv - Developmental Biology 2019Quote: TUNEL assay to label apoptotic and dead cells was performed using the ApopTag® Red In Situ Apoptosis Detection Kit (Millipore, cat. #S7165) following the manufacturer’s instructions with minor modifications ...
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bioRxiv - Developmental Biology 2019Quote: Apoptosis induced DNA damage was measured using the ApopTag® Peroxidase In Situ Apoptosis Detection Kit (cat no. S7100, EMD Millipore, Temecula, CA) following the manufacturer’s protocol for paraffin-embedded tissue ...
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bioRxiv - Molecular Biology 2019Quote: ... Hybridization and all other procedures followed the manufacturer’s instructions using the DIG High Prime DNA Labeling and Detection Starter Kit-I (Sigma Aldrich, St. Louis, MO).
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bioRxiv - Cancer Biology 2021Quote: ... EdU detection reagents were then added for 2 hours at room temperature in the dark (Click-iT™ Assay Kit, Sigma-Aldrich #C10337). Nutlin was used as a positive control (Selleckchem #S7101) ...
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bioRxiv - Cell Biology 2021Quote: The peptide/ZO1 interaction was detected in situ using the Duolink secondary antibodies and detection kit (Sigma, St Louis, MO, Cat: 92002, 92004) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2023Quote: The TUNEL assay was performed on paraffin-embedded sections and fifty-micron-thick free-floating frozen sections according to the manufacturer’s instructions but with the time of some steps lengthened to ensure good penetration of the reagents into the tissue (ApopTag® Fluorescein In Situ Apoptosis Detection Kit, Millipore-Sigma, Cat # S7110). Sections were post-fixed for ten minutes at room temperature in 2:1 ethanol:acetic acid ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell lines were regularly checked for mycoplasma contamination using Venor™ GeM Mycoplasma Detection Kit (Sigma-Aldrich -Aldrich Co., Catalog no. MP0025-1KT).
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bioRxiv - Pathology 2023Quote: Multicolor in situ PLA was performed using the Duolink PLA Multicolor detection kit (Duolink® PLA® Multicolor Reagent Pack, DUO96000, Sigma-Aldrich) as described previously(58 ...
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bioRxiv - Developmental Biology 2020Quote: ... Indexing and amplification was done using the KAPA HiFi HotStart PCR kit (Sigma-Aldrich, KK1508) with Index oligonucleotides (sequences were adapted from Illumina).
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bioRxiv - Molecular Biology 2020Quote: ... The DNA was purified using the High Pure PCR Product Purification Kit (11732676001 Sigma-Aldrich). Quantitative Real Time PCR reactions were performed with the TB Green™ Premix Ex Taq™ II (TAKARA ...
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bioRxiv - Molecular Biology 2021Quote: ... A cDNA library was generated using the cDNA Library Kit from Thermo Fisher Scientific (catalog A48571) and the Reverse transcription PCR kit from Millipore Sigma (Product No. HSRT100), with specific primers were designed for each target gene (Supplementary Table 3) ...
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bioRxiv - Microbiology 2021Quote: ... The amplicons were purified using GFX PCR DNA and Gel Band Purification Kit (Sigma-Aldrich), according to the manufacturer ...
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bioRxiv - Microbiology 2022Quote: ... All cell lines were tested for mycoplasma using Lookout Mycoplasma PCR kit (Sigma-Aldrich MP0035).
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bioRxiv - Evolutionary Biology 2022Quote: ... Amplified fragments were purified using spin columns (GenElute TM PCR Clean-Up Kit, Sigma- Aldrich) and were checked on agarose gel electrophoresis ...
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bioRxiv - Genomics 2019Quote: ... Tail snips were prepared using the REDExtract-N-Amp Tissue PCR Kit (Sigma-Aldrich XNAT) and were screened for Ube3a deletion using PCR primers spanning the expected deletion site ...
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bioRxiv - Genomics 2022Quote: ... Libraries were purified using the High Pure PCR Production Purification Kit (11732676001, Roche / Sigma-Aldrich). Libraries were validated on an Agilent 2100 and quantified using quantitative PCR (Q-PCR) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the ChIP-PCR assay was performed using the Chromatin Immunoprecipitation (ChIP) Assay Kit (Millipore, 3753379). The genomic DNA crossed with Foxo1a protein was immunoprecipitated by using 5 μg Anti-6×His tag antibody (abcam ...
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bioRxiv - Physiology 2023Quote: ... and PCR was performed using KOD Xtreme™ HotStart DNA Polymerase Kit (71975-3, Novagen) following manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... mm9) amplified by the PCR DIG Probe Synthesis Kit (Cat #11636090910, Sigma-Aldrich, MO, USA). The hybridized probe was immunodetected with antidigoxigenin Fab fragments conjugated to alkaline phosphatase (Cat # 11093274910 ...
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bioRxiv - Developmental Biology 2022Quote: ... Mice were genotyped by PCR on tail genomic DNA using Red-N extract kit (Sigma), with primers indicated in Supplemental table S3.
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bioRxiv - Cell Biology 2023Quote: ... DNA purification was performed using a SpinPrep™ PCR Clean-up Kit (Merck Millipore, Germany). DNA fragments were assayed by qRT-PCR using the primer sequences listed below ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA extraction was performed using Extract-N-Amp(tm) Tissue PCR Kits (Sigma/Aldrich). For cDNA synthesis ...
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bioRxiv - Plant Biology 2023Quote: ... qRT-PCR was performed using the KAPA SYBR Fast qPCR Kit (KK4621, Sigma-Aldrich, USA) and universal reverse primer (Table S2) ...
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bioRxiv - Cell Biology 2024Quote: ... The ChIP-PCR assay was performed using the Chromatin Immunoprecipitation (ChIP) Assay Kit (Millipore, 3753379) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... washed two times in M9 and one final time in M9 including 1 mM cycloheximide (Sigma). Samples were then washed with lysis buffer prepared in nuclease free water (10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES ...
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bioRxiv - Immunology 2021Quote: ... Colour detection was developed using TMB liquid substrate (Sigma-Aldrich), which was subsequently stopped using 1M sulphuric acid ...
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bioRxiv - Evolutionary Biology 2019Quote: ... an additional step of probe detection with Cy3-Streptavidin (Sigma) was applied ...
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bioRxiv - Synthetic Biology 2019Quote: ... Color detection was developed using ABTS liquid substrate (Sigma-Aldrich), which was subsequently stopped using 1 % SDS ...
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bioRxiv - Synthetic Biology 2019Quote: ... Color detection was developed using ABTS liquid substrate (Sigma-Aldrich) which was subsequently stopped with 1 % SDS ...
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bioRxiv - Genomics 2021Quote: ... followed by detection with 3,3′-Diaminobenzidine (catalog# D4293, Sigma-Aldrich,), counterstaining with Mayer’s hematoxylin ...
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bioRxiv - Neuroscience 2021Quote: ... Anti-TUJ1 (for TUBB3 detection; 1:1000; T2200, Sigma-Aldrich), anti-GAP-43 (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were revealed using chemiluminescent detection (Immobilon Western, Millipore WBKLS0100) and visualized using a Kodak GEL Logic 1500 Imaging System with Transilluminator.
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bioRxiv - Microbiology 2021Quote: ... proteins were visualized using chemiluminescent peroxidase substrat detection reagents (Sigma), and acquired by the ChemiDoc XRS+ System (BioRad) ...
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bioRxiv - Molecular Biology 2022Quote: ... and the Detection Reagent Red were purchased from Sigma-Aldrich. PLAs were performed as previously described (89 ...
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bioRxiv - Cell Biology 2022Quote: ... the detection was performed using Immobilon western chemiluminescent substrate (Millipore).
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bioRxiv - Cell Biology 2022Quote: ... Signals were visualized with chemiluminescence detection reagent (Sigma, Bio-Rad) and autoradiography films (Blue Devil ...
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bioRxiv - Immunology 2021Quote: ... following detection using p-nitrophenyl phosphate (pNPP) substrate (Sigma, Israel).
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bioRxiv - Genetics 2019Quote: ... incubated with 2 ml high sensititivity ECL detection solution (Millipore), and blots were visualized on a GeneGnome chemiluminsescence imaging system.
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bioRxiv - Cell Biology 2020Quote: ... The protein bands were visualized by ECL detection system (Millipore) or ODYSSEY (LI-COR).
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bioRxiv - Immunology 2020Quote: ... followed by detection with secondary anti-Mouse IgG Peroxidase (Sigma) and Vector NovaRED substrate kit for peroxidase (Vectorlabs) ...