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Citations for Millipore Sigma :
1301 - 1350 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... for 15 min at RT and permeabilized in 0.25% Triton X-100 (Sigma-Aldrich) for 20 min at RT ...
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bioRxiv - Microbiology 2023Quote: ... The mixture was cooled overnight at RT and filtered through 0.22 µm filter (Millipore). The insoluble PG sacculi were collected on the filters ...
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bioRxiv - Physiology 2023Quote: ... for 1h at RT and developed using Immobilon™ Western Chemiluminescent HRP Substrate (Millipore). Detection and quantification of chemiluminescence intensities were quantified by using ChemidocTM imaging system and Image Lab 5.2.1 software (BioRad) ...
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bioRxiv - Molecular Biology 2023Quote: ... permeabilized for 1 h at RT in PBS + 0.3% Triton X-100 (Sigma-Aldrich) (PBST) ...
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bioRxiv - Immunology 2024Quote: ... a 5min incubation at RT with 4’,6-diamino-2-phenylindole (DAPI, Sigma-Aldrich); and three 5min washes with PBS ...
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bioRxiv - Plant Biology 2021Quote: ... PCR reactions were performed with the Readymix Red Taq PCR reactive mix (Sigma-Aldrich), Phusion high-fidelity DNA polymerase (Fermentas ...
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bioRxiv - Microbiology 2019Quote: ... PCR reactions were performed with the Readymix Red Taq PCR reactive mix (Sigma-Aldrich) or with the Phusion high-fidelity DNA polymerase (Fermentas ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR was performed using REDTaq® ReadyMix™ PCR Reaction Mix (R2523, Merck-SIGMA) using previously published primers (Charalambous et al ...
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bioRxiv - Cell Biology 2021Quote: ... The gDNA extractions and PCR verifying integration of the fluorescent tags into the genomic loci was performed using the REDExtract-N-Amp Tissue PCR kit (Sigma-Aldrich). PCR amplicons were run on 1 or 2% agarose gels in TBE and gels were imaged with Bio-Rad Gel ChemiDoc XRS system and Quantity One software.
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bioRxiv - Cell Biology 2021Quote: ... and Spo74-mScarlet were generated at their endogenous genome locus by transformation with PCR tagging cassettes of indicated C-terminal tags using the PEG/lithium acetate yeast transformation kit (Millipore-Sigma, YEAST1). Plasmids with 2XGFP-Atg8 in pRS303 ...
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bioRxiv - Systems Biology 2022Quote: ... and cDNA fragments were amplified by PCR according to the manufacturer’s protocol (GenomePlex Single Cell Whole Genome Amplification Kit, Sigma Aldrich WGA4). The amplified libraries were run on a bioanalyzer to assess quality (Agilent ...
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bioRxiv - Cell Biology 2022Quote: ... ear notches were collected from mouse pups to extract genomic DNA using the RED Extract-N-Amp Tissue PCR Kit (Sigma-Aldrich). Ear notches were incubated in the tissue preparation (25 μl/sample ...
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bioRxiv - Pathology 2022Quote: ... Pf2-HA_OE and pf2-HA_KO ChIP and input control samples) was recovered from Proteinase K treated samples using the GenElute PCR purification kit (Sigma-Aldrich).
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bioRxiv - Developmental Biology 2019Quote: ... Yolk sacs from each of the dissected embryos were collected and genomic DNA preparation was done using Extract-N-Amp tissue PCR kit (Sigma-XNAT2). Placenta tissues were collected in RLT buffer and RNA was extracted using RNAeasy Mini Kit (Qiagen – 74104) ...
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bioRxiv - Developmental Biology 2019Quote: Genomic DNA samples were prepared using tail tissues or embryonic tissues from the mice using the REDExtract-N-Amp Tissue PCR kit (Sigma-Aldrich). Genotyping was done using REDExtract-N-Amp PCR ReadyMix (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... R-domain phosphorylation sites and interacting residues mutants were generated by site-directed mutagenesis using a Q5-New England Biolabs PCR mutagenesis kit together with primers synthesized from Millipore sigma and verified by sequencing (Elim Biopharmaceuticals ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR sex genotyping reactions were performed in a final volume of 25 μl with the following reagents and volumes per sample: 12.5 μl KAPA2G Fast HotStart DNA Polymerase (5 U/μL) from the KAPA2G Fast HotStart PCR Kit (Sigma-Aldrich, KK5601), 1 μl of 12.5 μM Ube forward primer and 1 μl of 12.5 μM Ube reserve primer ...
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bioRxiv - Cell Biology 2023Quote: ... elegans genomic DNA extracted from 8-10 adult worms using the Extract-N-Amp™ Tissue PCR Kit (Millipore Sigma, MA) as previously described38 then sequenced ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was extracted from mice ear tissues (or tail for embryos) using Extraction « Extract-N-Amp PCR » kit (Sigma #XNAT2-1KT) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR1 products for each tumor were individually column-purified with a GenElute™ PCR Clean-Up Kit (Sigma-Aldrich NA1020-1KT). For PCR2 ...
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bioRxiv - Developmental Biology 2023Quote: ... Library preparation was then performed using 200 ng of 4C-template DNA with the Expand Long Range PCR kit (Millipore, 4829042001) under specific PCR conditions (94 °C for 2 min ...
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bioRxiv - Neuroscience 2023Quote: DNA from larval tails and juvenile and adult tail fins was extracted using the Extract-N-Amp Tissue PCR Kit (Millipore Sigma). After verifying SNP location with Sanger sequencing at Azenta Life Sciences ...
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bioRxiv - Developmental Biology 2024Quote: ... or ear biopsies (3-week-old pups) was used for PCR based genotyping using the REDExtract-N-Amp kit (Sigma-Aldrich) with the primers listed in STable5 ...
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bioRxiv - Genomics 2020Quote: Total RNA was extracted from leaf tissue collected at each time point for all species using the Spectrum Plant Total RNA Kit (Sigma-Aldrich Co., St. Louis, MO, USA) following Protocol A ...
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Molecular coevolution of nuclear and nucleolar localization signals inside basic domain of HIV-1 TatbioRxiv - Evolutionary Biology 2021Quote: ... The membranes were washed three times with PBS (5 min each time) and were then incubated with secondary peroxidase-conjugated antibody (1:15,000; Sigma-Aldrich, St. Louis, MO, USA). The antibody-bound proteins were detected using Pierce ECL western blotting substrate (Thermo Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... Membranes were washed with TBST buffer (three times, 7 minutes each) and incubated with anti-mouse-HRP or anti-rabbit-HRP (Sigma-Aldrich; 20,000 times dilution) for 1 hour ...
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bioRxiv - Molecular Biology 2023Quote: ... 1×PCR buffer (Sigma P2192), 400 nM of primers (Table S.6 ...
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bioRxiv - Microbiology 2023Quote: ... 1× PCR Buffer (Sigma-Aldrich), 3 mM MgCl2 ...
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bioRxiv - Immunology 2022Quote: ... cells were washed three times with Tyrode’s Salts (Sigma-Aldrich), stained for 40 min with 2 μM Fura Red™ ...
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bioRxiv - Immunology 2022Quote: ... The plate was washed four times before TMB (Sigma Aldrich) was added ...
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bioRxiv - Molecular Biology 2020Quote: ... and flown multiple times over FLAG-M2 affinity beads (Sigma) which were pre-equilibrated in lysis buffer and packed in a gravity flow column ...
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bioRxiv - Neuroscience 2020Quote: ... and concentrated three times with a Centriprep centrifugal filter (Millipore) at 1,220 × g for 5 min each and with an Amicon Ultra centrifugal filter (Millipore ...
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bioRxiv - Biophysics 2020Quote: ... After washing three times with PBS (Sigma-Aldrich, D8537-500ML), the surfaces were incubated with 1 ng/ µl biotinylated alpha-tubulin (Abcam ...
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bioRxiv - Cancer Biology 2021Quote: ... and destained multiple times in 75 mM ammonium bicarbonate (Sigma) and 40% EtOH (1:1 ...
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bioRxiv - Microbiology 2020Quote: ... several times before reducing the protein using 5mM dithiothreitol (Sigma) at 55°C for 45 min and alkylating in 14mM iodoacetamide (Sigma ...
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bioRxiv - Biophysics 2020Quote: ... After washing the coverglasses three times with deionized water (Millipore), 300 μL of yeast cells were incubated on the coverglass at an optical density (OD ...
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bioRxiv - Biochemistry 2022Quote: ... Agarose beads were washed 4 times on IP columns (Sigma) with a washing buffer (25mM Tris/HCl pH 7.4 ...
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bioRxiv - Immunology 2022Quote: ... The plate was washed four times before TMB (Sigma Aldrich) was added ...
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bioRxiv - Developmental Biology 2022Quote: ... sections were fixed 2 times with 4% PFA (Sigma, 158127) for 15 minutes followed by subsequent incubation steps in 0.1% Triton TX-100 (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... and washed three times with RPMI 1640 (Sigma Aldrich, UK) to separate the erythrocytes from the other blood components ...
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bioRxiv - Genomics 2021Quote: ... and washed 4X times using 1 mL ethanol (Sigma, #E7023) at 80% concentration in each wash ...
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bioRxiv - Immunology 2020Quote: ... Plates were then washed six times with PBS (Sigma-Aldrich) and incubated for 2 hr at room temperature with biotinylated anti-IFN-γ (clone mAb-7B6-1 ...
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bioRxiv - Microbiology 2022Quote: ... washed one time with Na2S2O3 (20%) (Sigma-Aldrich, Darmstadt, Germany) and 5 times in sterile distilled water ...
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bioRxiv - Plant Biology 2022Quote: ... The pellet was washed three times in 90% Percoll (Sigma) buffered with 50 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2024Quote: ... washed 3 times in 0.1% Tween-20 (Sigma Aldrich P9416), permeabilized for 4 hrs in 0.5% Triton X-100 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and washed three times with 1x sterile PBS (Sigma Aldrich). Trypsin-DNAse solution (0.25 %-250 µg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed three times with PBS and DAPI (Sigma) was added to the wells ...
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bioRxiv - Cell Biology 2023Quote: ... fixed at different time points with 4% paraformaldehyde (PFA, Sigma) diluted in MT-stabilising buffer (MTSB ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed three times in PBS (Sigma-Aldrich, UK) and then processed as fixed samples as described ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed five times and OPD color reaction (Sigma) was added to each well ...