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Citations for Millipore Sigma :
751 - 800 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... reverse transcribed with AMV RT (Sigma 10109118001), and then cDNA were quantified in multiplex TaqMan qPCR reactions using commercially available probe sets (Thermo Fisher 4453320 ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 1U ThermaStop-RT (Sigma-Aldrich) per 50U SSIII to increases RT-PCR specificity ...
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bioRxiv - Developmental Biology 2021Quote: ... Rats were screened for the presence of mutations by PCR using tail-tip DNA samples (RED extract-N-Amp Tissue PCR Kit, Millipore-Sigma) as described previously (1 ...
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bioRxiv - Immunology 2021Quote: ... we extracted genomic DNA from mouse tails and performed PCR with REDExtract-N-Amp™ Tissue PCR Kit following the manufacturer’s instructions (Sigma-Aldrich) and analyzed samples on agarose gel ...
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bioRxiv - Biochemistry 2019Quote: ... The DNA substrate was generated by PCR and the products were purified using a GenElute PCR Clean-up kit (Sigma-Aldrich). If required ...
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bioRxiv - Biochemistry 2021Quote: ... The DNA substrate was generated by PCR and the products were purified using a GenElute PCR Clean-up kit (Sigma-Aldrich). If required ...
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bioRxiv - Developmental Biology 2020Quote: ... Rats were screened for the presence of mutations by PCR using tail-tip DNA samples (RED extract-N-Amp Tissue PCR Kit, Sigma-Aldrich) as previously described (41,44) ...
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bioRxiv - Developmental Biology 2020Quote: ... Rats were screened for the presence of mutations by PCR using tail-tip DNA samples (RED extract-N-Amp Tissue PCR Kit, Sigma-Aldrich) as described previously (Rumi et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... Genotypes were verified by PCR of tail DNA using the RedExtract-N-Amp Tissue PCR Kit (Sigma Aldrich 254-457-8).
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bioRxiv - Cancer Biology 2023Quote: ... Routine genotype analysis of mice was performed by PCR assay on DNA purified from tail biopsies (Extract N-Amp Tissue PCR kit, Sigma Aldrich). Wild-type and KO mutant alleles were distinguished using primers available on request ...
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bioRxiv - Synthetic Biology 2023Quote: ... Real-Time PCR reactions were performed in 20 µL mixtures containing 10 µL KAPA SYBR FAST qPCR Master Mix (2X) (Sigma-Aldrich, KK4605), 200 nM final concentration of species-specific rpoD forward and reverse primer and up to 3 µL of sample template ...
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bioRxiv - Cell Biology 2020Quote: ... Cell death was evaluated by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) (ApopTag Plus Peroxidase In Situ Apoptosis Detection Kit, Millipore-Sigma). Cell proliferation was evaluated by Ki-67 immunostaining (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2020Quote: ... Terminal deoxy-nucleotidyl transferase dUTP nick end labeling (TUNEL) was performed on transduced human islets (Apoptag In situ Apoptosis Detection Kit; Millipore) as per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: PLA was performed on cultured granulosa cells using the Duolink PLA Probes and PLA Fluorescence in situ Detection Kit Red (Sigma) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: Terminal deoxynucleotidyl transferase (TdT) dUTP nick-end labeling (TUNEL) was performed on whole-mount larvae (ApopTag Rhodamine In situ Apoptosis Detection Kit; Millipore). Embryos were fixed ...
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bioRxiv - Neuroscience 2020Quote: ... Sandwich ELISA kits for quantitative detection of human Aβ 1-40 peptides in a flexible 96 well format were purchased from Millipore (cat ...
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bioRxiv - Cell Biology 2021Quote: Carbonyl groups introduced into protein side chains (reflecting aldehyde-induced oxidative modification of proteins) were measured by an OxyBlot protein oxidation detection kit (Millipore) by equally splitting the heart homogenate into two samples ...
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Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... GP:NPC1 interaction was detected by applying the Duolink® In Situ Detection Reagents Red kit following the manufacturer’s instructions (Sigma). After removing excess reagents ...
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bioRxiv - Genetics 2022Quote: ... Cell death analysis was performed using the ApopTag® Fluorescein in situ Apoptosis Detection Kit (Sigma-Aldrich, Cat. No. S7110) following manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) histochemistry was performed using the ApopTag Peroxidase In Situ Apoptosis Detection Kit (Merck Millipore), according to the instructions of the manufacturer ...
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bioRxiv - Neuroscience 2020Quote: ... The proximity ligation reaction and amplification of signal were performed according to the manufacturer’s protocol using the Duolink Detection Kit with PLA PLUS and MINUS probes for mouse and rabbit antibodies (Duolink, Sigma). DAPI stain was used to detect cell nuclei ...
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bioRxiv - Developmental Biology 2021Quote: Cell death was assessed using the TdT-mediated dUTP nick end-labeling (TUNEL) method (ApopTagPeroxidase In Situ Apoptosis detection kit, Millipore) as per manufacturers’ instructions ...
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bioRxiv - Physiology 2020Quote: Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) was performed on whole-mount larvae (ApopTag Fluorescein In Situ Apoptosis Detection Kit; Millipore) as previously described (Lambert et al. ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Citrulline-containing proteins were modified on the membrane and detected using the anti-modified citrulline detection kit (Millipore, 17-347) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: Protease assays were performed with a protease fluorescent detection kit using casein labeled with fluorescein isothiocyanate (FITC) as the substrate following the manufacturer’s instructions (PF0100, Sigma-Aldrich). Briefly ...
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bioRxiv - Developmental Biology 2022Quote: Whole mount TUNEL was performed as described previously (Causeret et al., 2011) using the Apoptag Peroxidase in situ Apoptosis Detection kit (Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were authenticated by STR analysis (Suzhou, China) and tested for negative mycoplasma contamination using Mycoplasma Detection Kit (Sigma). MDA-MB-231 ...
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bioRxiv - Biophysics 2024Quote: ... The slides were then rinsed with PBS (3×) and then stained with an in situ cell death detection (FITC-TUNEL) staining kit (Millipore), an AlexaFluor-647 anti-collagen antibody (NovusBio ...
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bioRxiv - Genomics 2023Quote: ... Cells were validated by STR analysis and regularly confirmed to be mycoplasma free by Venor GeM Mycoplasma Detection Kit (Milipore Sigma). 293T cells were cultured at 37 C ...
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bioRxiv - Molecular Biology 2023Quote: ... The ApopTag Peroxidase In Situ Apoptosis Detection Kit was used for Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) (Sigma Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: Apoptotic cell death was detected using the The ApopTag Plus Fluorescein In Situ Apoptosis Detection Kit (Sigma, S7111, supplemental methods).
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bioRxiv - Cell Biology 2023Quote: ... Mycoplasma contamination of cells was tested and ruled out with the LookOut Mycoplasma Detection kit (Sigma-Aldrich; catalog number: MP0035).
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bioRxiv - Cell Biology 2022Quote: ... Fixed cells were stained either for ALP activity using an ALP detection kit according to the manufacturer’s instructions (Sigma-Aldrich) or with crystal violet ...
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bioRxiv - Plant Biology 2022Quote: ... 3-6 µg total protein was derivatized with DNPH and subsequently detected with an anti-DNP antibody according to the manufacturer’s instruction of the OxyBlot Protein Oxidation Detection Kit (S7150, Sigma-Aldrich). Equal amounts of the derivatized protein samples were employed as loading control on a separate SDS-gel and stained with Coomassie or silver staining.
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bioRxiv - Developmental Biology 2023Quote: ... The resultant samples were load on SDS-PAGE and subjected to pIMAGO phospho-protein detection kit with fluor-680 (Sigma) detection kit and LI-COR Odyssey platform for western blot analyses of phosphorylated proteins (Millipore Sigma) ...
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bioRxiv - Plant Biology 2024Quote: ... Unique integration of the cassette in the mutants was then controlled by Southern blot using DIG-High Prime DNA Labelling and Detection Starter Kit I (Sigma). Primers used for these experiments are listed in Table S1 ...
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bioRxiv - Cell Biology 2024Quote: ... Terminal deoxy-nucleotidyl transferase dUTP nick end labeling (TUNEL) was performed on transduced human islets (Apoptag In situ Apoptosis Detection Kit; Millipore) as per the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... Amplification and label probe binding was performed after further washes with wash buffer A with the amplification reaction mixture containing Phi29 polymerase and the fluorophore-labeled detection oligo prepared according to the manufacturer’s recommendations (Duolink Detection reagents Red, Sigma) in a prewarmed humidified chamber at 37 °C for 100 min ...
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bioRxiv - Cell Biology 2023Quote: ... for 30 minutes at RT and permeabilized for 15 minutes at RT with 0.2% Triton 100 X buffer (Sigma). Calu-3 cells were fixed with 4% PFA for 20 minutes at RT and permeabilized for 15 minutes at RT with 0.2% Triton 100X buffer ...
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bioRxiv - Systems Biology 2024Quote: ... Reverse transcription (RT) was performed by the addition of 75μl RT reagents: 50ng/μl actinomycin D (Sigma-Aldrich, USA), 0.5mM dNTPs (ThermoFisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... samples were covered with a digestion solution (1% proteinase K, 20% v/v SDS [ThermoFisher, AM9823], 0.25% v/v triton-X [Sigma, T8787] in 2X SSC) and incubated at 37 °C for 24 hours ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA for reverse transcription polymerase chain reaction (RT-PCR) was isolated from 100 mg of oat leaves using TRI REAGENT® (Sigma-Aldrich, St. Louis, MO, USA) according to the provided protocol ...
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bioRxiv - Physiology 2020Quote: ... miR-150fl/Cdh5(PAC)-iCreERT2 mice were injected intraperitoneally with 100 µL of 5 mg/mL tamoxifen (Sigma, cat. no. T5648) or vehicle (12.5% vol/vol ethanol in peanut oil ...
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bioRxiv - Cancer Biology 2019Quote: ... mice were treated via tail vein injection with 80 μg of control miRNA or 5-FdU-miR-15a packaged using in vivo-jetPEI (Polyplus Transfection, Illkirch, France) or with 50 mg/kg of gemcitabine (Sigma Aldrich) via intraperitoneal injection (IP injection) ...
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bioRxiv - Molecular Biology 2023Quote: ... with either the wild type Cacna2d2-3’UTR or the Cacna2d2 3’UTR bearing mutations in the miR-423-5p binding sites (Cacna2d2 mut 3’UTR) in HEK 293 cells (Millipore Sigma). Briefly ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We purified the PCR product using the GenElute Plasmid Miniprep Kit (Sigma Aldrich) and used it as a template to insert the mutation into OmpF— transformed with pkD46 ...
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bioRxiv - Microbiology 2021Quote: ... The amplified cDNA was purified with a GenElute PCR cleanup kit (Sigma-Aldrich), and concentration ...
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bioRxiv - Genomics 2022Quote: ... mm9) ampli?ed by the PCR DIG Probe Synthesis Kit (Sigma-Aldrich, 11636090910). The hybridized probe was immunodetected with antidigoxigenin Fab fragments conjugated to alkaline phosphatase (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... PCR was performed using the KOD Hot Start DNA polymerase kit (Novagen, TOYOBO). The list of primers used for RT PCR is indicated on table 3.
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bioRxiv - Developmental Biology 2021Quote: ... Genomic DNA was extracted using the REDExtract-N-Amp Tissue PCR Kit (Sigma): 50 µl of extraction solution and 12.5 µl of tissue preparation solution was added to each embryo ...