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Citations for Millipore Sigma :
801 - 850 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... and PCR on extracted DNA (GenElute Mammalian Genomic DNA Miniprep Kit; Sigma-Aldrich). Selected clones were finally adapted to growth in suspension in Expi293 Expression Medium (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... The amplified cDNA was purified with the GenElute PCR cleanup kit (Sigma-Aldrich), and the quality and quantity of ds-cDNA was determined with NanoDrop 1000 and a 2100 Bioanalyzer using the Agilent DNA 7500 kit (Agilent Technologies) ...
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bioRxiv - Physiology 2022Quote: PCR reactions were performed with the KAPA3G Plant kit (Sigma Aldrich, MO, USA) under the following conditions ...
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bioRxiv - Physiology 2023Quote: ... DNA extraction was done using REDExtract-N-Amp Tissue PCR kit (XNAT, Sigma). For the PCR reaction ...
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bioRxiv - Microbiology 2024Quote: ... 0.4 µl 10 µM reverse tailed specific primer and 12.72 µl PCR grade water (contained in the Kap2G Robust PCR kit Sigma Catalogue No. KK5005) per sample and 18 µl added to each well to be used in a 96-well plate followed by 2 µl of DNA and mixed ...
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Dual functionality of the TasA amyloid protein in Bacillus physiology and fitness on the phylloplanebioRxiv - Microbiology 2019Quote: ... was washed 3 times with PBS and processed using the CelLytic MEM protein extraction kit from Sigma. Briefly ...
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bioRxiv - Pathology 2021Quote: ... followed by ligation and amplification with Duolink detection reagent Texas Red according to the manufacturer’s instructions (Duolink In Situ detection reagent-Red, Sigma Aldrich). The samples were then imaged in a confocal laser scanning microscope (Leica TCS SP5 ...
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bioRxiv - Developmental Biology 2020Quote: ... Sections were washed two times with PBS and incubated for two hours at RT with corresponding secondary antibodies and subsequently incubated for 30 min at RT with DAPI (Sigma). Stained sections were washed two times with PBS and mounted with either Fluorsave (Chemicon ...
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bioRxiv - Cell Biology 2021Quote: ... followed by detection using chemiluminescent substrates from Millipore. LAS4000 (Fujufilm-GE ...
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bioRxiv - Cell Biology 2020Quote: ... Chemiluminescence detection was performed using Immobilon reagent (Millipore) and the Gel-Doc™ XR+ system (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... and Detection Reagent Red were purchased from Sigma. PLA was performed on HEK293T cells as previously described (29) ...
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bioRxiv - Cell Biology 2020Quote: ... Duolink In Situ Detection Reagents Red (SIGMA, DUO92008); Duolink In Situ Detection Reagents Brightfield (SIGMA ...
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bioRxiv - Molecular Biology 2020Quote: ... amplification using Duolink In Situ Detection Reagents (Sigma) was performed at 37 °C for 100 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Detection was performed using Immobilion Western (Millipore #WBKLS0500).
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bioRxiv - Molecular Biology 2020Quote: ... before detection using HRP-conjugated Secondary Antibody (Sigma) and ECL Western Blotting substrate (Pierce ...
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bioRxiv - Cancer Biology 2019Quote: ... and chemiluminescent HRP detection (substrate: Millipore Sigma #WBLUF0100). Blocking was done overnight at 4°C using 5% (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... Duolink In Situ Detection Reagents Brightfield (SIGMA, DUO92012).
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bioRxiv - Neuroscience 2022Quote: ... Signals were developed using ECL-plus detection (Millipore). Images were acquired on X-ray films (Denville Scientific) ...
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bioRxiv - Pathology 2023Quote: ... Signals were visualized with chemiluminescence detection reagent (Sigma) on a Chemidoc (Biorad ...
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bioRxiv - Pathology 2023Quote: ... Signals were visualized with chemiluminescence detection reagent (Sigma) on a Chemidoc (Biorad ...
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bioRxiv - Plant Biology 2024Quote: ... anti- mouse antibody for HRP detection (Sigma-Aldrich), and anti-rat antibody for HRP detection (Sigma- Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... were used for ECL detection Reagents (Sigma-Aldrich) and scanning on the Amersham Imager 600 Instrument (GE Healthcare) ...
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bioRxiv - Bioengineering 2024Quote: ... and detection with BCIP/NBT substate (Sigma-Aldrich) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Detection was performed by ECL (GS009-4; Millipore) solution and imaged using the Tanon5200 detection system finally ...
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bioRxiv - Cancer Biology 2021Quote: To detect apoptosis in the cells after bergamottin treatment In Situ cell death detection kit from Sigma-Aldrich ((St. Louis, MO) was used ...
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bioRxiv - Cell Biology 2020Quote: ... Cell death was evaluated by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) (ApopTag Plus Peroxidase In Situ Apoptosis Detection Kit, Millipore-Sigma). Cell proliferation was evaluated by Ki-67 immunostaining (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2021Quote: ... were obtained using terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) assay (82) (based on in Situ Cell Death Detection Kit, 11684795910, red fluorescence; Sigma-Aldrich) on sections derived from rd1 and C3H retinal explant cultures ...
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bioRxiv - Cell Biology 2021Quote: ... The TUNEL assay was performed on the testis sections (5 μm) with ApopTag plus peroxidase in situ apoptosis detection kit (Millipore, S7101).
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bioRxiv - Cell Biology 2020Quote: ... Medium was changed every 24 h and plates were stained for alkaline phosphatase activity to assure the efficiency of reprogramming (AP detection kit, Sigma-Aldrich). Once colonies were picked ...
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bioRxiv - Microbiology 2021Quote: MCDK cell viability following exposure to GO-AgNP supernatants or vehicle was assessed using a Cytotoxicity Detection Kit (Millipore Sigma #11644793001). Prior to testing ...
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bioRxiv - Neuroscience 2022Quote: ... The paraffin sections were assayed for apoptotic cells using the ApopTag Plus Fluorescein in Situ Apoptosis Detection Kit (Millipore, Cat. #S7111) as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... apoptotic cell death in 1-day old zebrafish whole-mounts was detected according to a modification of the ApopTag® Fluorescein In Situ Apoptosis Detection Kit (Merck Millipore) protocol ...
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bioRxiv - Cell Biology 2024Quote: Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay in imaginal wing discs was performed using the ApopTag red in situ apoptosis detection Kit (#S7165, Sigma-Aldrich), following the manufacturer’s procedure ...
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bioRxiv - Molecular Biology 2024Quote: The proximity ligation assay (PLA) was conducted to determine potential protein–protein interactions using the Duolink in situ detection kit (Sigma-Aldrich) in accordance with the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The ApopTag Peroxidase In Situ Apoptosis Detection Kit was used for Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) (Sigma Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: TUNEL assays were performed after secondary antibody staining by using the In Situ Cell Death Detection Kit TMR red (Millipore #12156792910). Embryos were incubated for 4 hours at room temperature (RT ...
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bioRxiv - Cancer Biology 2023Quote: C6 cells were treated with indicated nanoformulations for fixed time points followed by Annexin V/ propidium iodide (PI) staining using Annexin V-FITC Apoptosis Detection Kit II (Cat # PF032, Sigma-Aldrich), as per manufacturer protocol ...
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bioRxiv - Cancer Biology 2024Quote: Cells were seeded in VitroGel at 50% confluence on chamber slides for PLA (Proximity Ligation Assay) using the Duolink in situ Detection Reagents Red kit (DUO92008, Sigma-Aldrich), following the given protocol ...
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bioRxiv - Cell Biology 2023Quote: ... After fixation cells were placed in hybridization buffer (5% dextran sulfate, 50% formamide, 5× SSC, 100 μg/ml heparin, 100 μg/ml sonicated salmon sperm DNA (Sigma-Aldrich, cat. No. D9156),0.1% Tween-20 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then spun down at 7,000g for 8 minutes and resuspended in 100 μL secondary hybridization buffer (10% Formamide, 2X SSC, 10% Ficoll PM-400 (Sigma-Aldrich F5415-25 mL)) ...
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bioRxiv - Cancer Biology 2023Quote: ... we mounted the slides in an anti-bleach imaging buffer containing 2X SSC/10 mM Tris-HCl/10 mM Trolox (Sigma Aldrich, cat. no. 238813)/37 ng/μL glucose oxidase (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... for 3 min at 95 °C, and then mixed with 50 μL hybridization buffer H (4x SSC, 4 mg/ml acetylated BSA (Sigma Fluka Aldrich, B8894-1.5mL), 20 mM vanadyl ribonuclease complex) ...
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bioRxiv - Microbiology 2020Quote: ... RT SigmaFast O-phenylenediamine (OPD) substrate (P9187, Sigma) was added to the wells after six additional washes to develop the colorimetric reaction ...
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bioRxiv - Physiology 2021Quote: ... For the RT-qPCR the Kappa Mix (Sigma) was used and the primers concentration was 10 µM ...
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bioRxiv - Cell Biology 2024Quote: ... and 50 mg Fast Blue RT (Sigma-Aldrich) in 100 ml of 50 mM 2-amino-2-methyl-1,3-propanediol buffer (pH 9.8) ...
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bioRxiv - Cell Biology 2023Quote: ... permeabilized at RT with 0.25% TritonX100 (Sigma #T9284) for 10 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Genotypes of the offspring were verified by PCR amplification of genomic ear DNA using the commercially available REDExtract-N-Amp™ Tissue PCR Kit (#R4775; Sigma-Aldrich, Germany) and primers designed to identify the presence or absence of the knock-in allele ...
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bioRxiv - Synthetic Biology 2021Quote: ... Green fluorescence images were used for the detection of sfGFP and red fluorescence images were used for the detection of the sulforhodamine B dye (230162, Sigma-Aldrich), used to detect the correct flow of inputs.
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bioRxiv - Cancer Biology 2020Quote: Reverse transcription (RT) reactions were carried out using 1 µg of RNA using Transcriptor RT enzyme (Catalog No. 03531287001) from Sigma Aldrich. IR minigene PCRs were performed as previously reported ...
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bioRxiv - Neuroscience 2024Quote: ... for 10 min at RT and blocked for 30 min at RT in 1% bovine serum albumin (BSA, A9647, Sigma-Aldrich). For overnight incubation at +4°C the following primary antibodies were used ...