Labshake search
Citations for Millipore Sigma :
9901 - 9950 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: ... the protein was concentrated using an 10K MWCO centrifugal concentrator (Amicon-15, Millipore) to 20 mg/mL and purified by size exclusion chromatography on a Superdex 75 10/300 GL column (GE Healthcare Life Sciences) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Proteins were then precipitated upon addition of 50% trichloroacetic acid (TCA, Sigma-Aldrich) to a final concentration of 5% ...
-
bioRxiv - Molecular Biology 2023Quote: ... Proteins coupled to streptavidin-coated magnetic beads were reduced with dithiothreitol (Sigma-Aldrich) and alkylated with iodoacetamide ...
-
bioRxiv - Cell Biology 2023Quote: ... protein was extracted from SC cells using lysis buffer (CelLyticTM M, Sigma-Aldrich) supplemented with Halt™ protease/phosphatase inhibitor cocktail (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2023Quote: ... The immunoprecipitated TFEB proteins were dephosphorylated by λ phosphatase (Cat# P9614, Sigma-Aldrich) following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... aliquots of protein were digested twice (overnight and 4h) with trypsin (Sigma-Aldrich) at 37°C ...
-
bioRxiv - Cancer Biology 2024Quote: ... The lysate was precleared with Protein G agarose beads (EMD Millipore 16-266) for FLAG immunoprecipitation or Protein A agarose beads (EMD Millipore 16-125 ...
-
bioRxiv - Pathology 2023Quote: AAA tissue protein was extracted using RIPA buffer with proteinase inhibitor (Sigma #MCL1). Protein quantification was done by Bradford assay ...
-
bioRxiv - Molecular Biology 2023Quote: Proteins were precipitated from sucrose fractions using trichloroacetic acid (TCA; Sigma-Aldrich, T6399). Briefly ...
-
bioRxiv - Molecular Biology 2023Quote: ... Eluates were concentrated with Microcon PL-30 (30,000 NMWL) protein concentrators (Millipore Sigma) and loaded onto 1 cm diameter ...
-
bioRxiv - Microbiology 2023Quote: ... Secreted proteins were precipitated by addition of 10 % trichloroacetic acid (TCA, Sigma-Aldrich) followed by centrifugation for 1 h at 4 °C ...
-
bioRxiv - Cell Biology 2023Quote: ... Flag-tagged proteins were bound to anti-FLAG M2 affinity gel (Sigma-Aldrich) for 1 h at 4°C ...
-
bioRxiv - Neuroscience 2023Quote: ... The proteins were eluted and concentrated with Amicon Centrifugal Filter Units 10K (Millipore) to remove excessive glutathione.
-
bioRxiv - Cancer Biology 2023Quote: ... samples were incubated with biotin-conjugated hyaluronic acid binding protein (HABP) (Sigma 385911), followed by streptavidin conjugated to AlexaFluor-488 (ThermoFisher S11223) ...
-
bioRxiv - Biophysics 2023Quote: ... The eluted protein was concentrated in a 50 kDa cut-off Vivaspin (Millipore) and run on a Superose 6 size exclusion column (GE Healthcare ...
-
bioRxiv - Cell Biology 2023Quote: ... Protein was concentrated with a 3.5 kDa MWCO Amicon ultracentrifuge filter device (Millipore). After filtering the protein through a syringe filter (0.45 µm) ...
-
bioRxiv - Cell Biology 2023Quote: ... Protein was concentrated with a 3.5 kDa MWCO Amicon ultracentrifuge filter device (Millipore) to a concentration of 5 mg/ml ...
-
bioRxiv - Cancer Biology 2023Quote: ... protein lysates were prepared using 1X RIPA buffer containing protease (#539131, Merck Millipore) and phosphatase (#524629 ...
-
bioRxiv - Cell Biology 2023Quote: ... The recombinant proteins were produced and purified on glutathione-agarose column (G4510, Sigma) for GST-tagged protein or on TALON-Cobalt beads column (635507 ...
-
bioRxiv - Neuroscience 2023Quote: ... the proteins were expressed in Escherichia coli strain BL21(DE3) (Novagen, Darmstadt, Germany) and cells were lysed with a cell disruptor (Avestin ...
-
bioRxiv - Molecular Biology 2023Quote: ... The purified mini-HA protein was concentrated by Amicon spin filter (Millipore Sigma) and filtered by 0.22 µm centrifuge Tube Filters (Costar) ...
-
bioRxiv - Neuroscience 2023Quote: ... Proteins were detected using an enhanced chemiluminescent detection system (ECL, EMD Millipore, #WBKLS0500) and the BioRad ChemiDoc MP (#12003153 ...
-
bioRxiv - Neuroscience 2023Quote: ... Protein concentration in supernatants was analyzed with the Bradford reagent (Sigma-Aldrich, USA) using bovine serum albumin in lysis buffer as a standard solution ...
-
bioRxiv - Molecular Biology 2023Quote: ... protein-bound beads were resuspended in 50mM TEAB pH 8 (Sigma-Aldrich, T7408) containing 1 μg trypsin (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2023Quote: ... and the protein concentration was determined by bicinchoninic acid (BCA) assay (Merck Millipore) and by UV-visible spectroscopy using an ε of 24 mM⁻¹·cm⁻¹ at 280 nm ...
-
bioRxiv - Immunology 2023Quote: ... The protein fractions with high purity were concentrated with a centrifugal concentrator (Millipore). TAB1 (468-504 ...
-
bioRxiv - Microbiology 2023Quote: ... Pre-prepared Protein A Dynabeads (incubated with 5 μg of S9.6 antibody (Millipore) in 1X PBS plus 0.5% BSA overnight at 4°C) ...
-
bioRxiv - Neuroscience 2023Quote: ... after which the interesting proteins were competitively eluted using 3XFLAG peptide (Sigma, F4799). The eluent was then incubated overnight at 4°C with Ni-NTA beads (Qiagen ...
-
bioRxiv - Immunology 2023Quote: ... Total protein content was quantified using the Bradford dye-binding method (Sigma, #B6916). A total of 50 μg of cellular proteins were mixed with 5x loading buffer and boiled for 5 min ...
-
bioRxiv - Molecular Biology 2023Quote: ... Protein samples were then concentrated using Amicon Ultra centrifugal filters (30k MWCO, Millipore). The protein concentration of eluates were estimated with a Bradford assay (BioRad ...
-
bioRxiv - Plant Biology 2023Quote: ... The over-expressed recombinant proteins were then coupled to CNBr-activated Sepharose (Sigma) at a ratio of 2.5 mg protein to 1 ml Sepharose slurry as described by the manufacturers.
-
bioRxiv - Molecular Biology 2023Quote: ... Fractions containing protein were concentrated in 50-kDa cutoff Amicon centrifugal filters (Millipore). Aliquots were flash frozen in liquid nitrogen and stored at −80 °C.
-
bioRxiv - Molecular Biology 2023Quote: ... fractions containing protein were concentrated in 10-kDa cutoff Amicon centrifugal filters (Millipore). Aliquots were flash frozen in liquid nitrogen and stored at −80 °C.
-
bioRxiv - Biophysics 2023Quote: ... the protein was digested online by a pepsin/FPXIII (Sigma-Aldrich P6887/P2143) mixed protease column maintained at 20 °C ...
-
bioRxiv - Biochemistry 2023Quote: ... Proteins were concentrated with ultrafiltration centrifugal devices of 50-kDa cutoff (Amicon, Millipore). Monodispersed fractions of proteins were collected from size-exclusion columns (Superdex 200 10/300 GL ...
-
bioRxiv - Biochemistry 2023Quote: ... 5 mM MgCl2 using a protein concentrator with a 10-kDa cutoff (Millipore, UFC801024 before loading onto a HiTrap Q HP column connected to an ÄKTA FPLC ...
-
bioRxiv - Bioengineering 2022Quote: ... Tissue punches were suspended in protein extraction RIPA lysis buffer (Sigma-Aldrich Corp) containing protease and phosphatase inhibitors cocktail (Roche ...
-
bioRxiv - Biochemistry 2023Quote: ... The protein was concentrated using a 50 kDa cut-off Amicon device (Millipore). The concentrated protein was centrifuged at 14,000 xg for 15 mins before injecting onto a Superdex 200 (10/300 ...
-
bioRxiv - Biochemistry 2023Quote: ... Then the protein was concentrated with centrifugal concentrators (UFC901096, Merck Millipore, Cork, Ireland), and loaded onto a SuperoseTM 6 Increase 10/300 GL gel-filtration column (29- 0915-96 ...
-
bioRxiv - Biochemistry 2022Quote: ... Samples were subsequently incubated for 2 h with Protein-G–Sepharose (Sigma-Aldrich). After 3 washes with lysis buffer ...
-
bioRxiv - Cell Biology 2022Quote: ... the isolated proteins were eluted in 25 µL 2x Laemmli buffer (Sigma Aldrich) by boiling for 5 min at 95 °C and the supernatant prepared for mass spectrometry measurement.
-
bioRxiv - Biophysics 2023Quote: ... Dialyzed protein was concentrated using an Amicon Ultra concentrator (30 kDa MWCO, Millipore) and aliquoted into 100 μl amounts and stored at −80 °C.
-
bioRxiv - Cell Biology 2023Quote: ... Mouse anti-Golgi 58K protein (formimidoyltransferase-cyclodeaminase) mAb (G2404) was from Sigma-Aldrich and used at a dilution of 1:50∼100 ...
-
bioRxiv - Neuroscience 2022Quote: ... protein was further concentrated using Amicon Ultra-0.5 mL centrifugal filters (Millipore Sigma) prior to use ...
-
bioRxiv - Immunology 2023Quote: ... and precipitated proteins competitively eluted with Flag peptide (150 ng/µl; F4799, Sigma). Lysates were denatured in SDS-PAGE buffer (4x Lämmli buffer (Bio-Rad ...
-
bioRxiv - Immunology 2023Quote: ... OVOL1 protein expression were determined by rabbit anti-human OVOL1 antibody (Sigma Aldrich).
-
bioRxiv - Developmental Biology 2023Quote: Protein was extracted from maternal liver using RIPA buffer (Sigma-Aldrich Corp., R0278). Protein concentration was determined by a Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Protein quantifications were normalized with the β-actin signal (A3854; Sigma-Aldrich®).
-
bioRxiv - Neuroscience 2023Quote: ... followed by rotating incubation with 20 µl of protein G-Agarose beads (Sigma) for 1 hour ...
-
bioRxiv - Molecular Biology 2023Quote: ... to which Magna ChIP™ Protein A+G Magnetic Beads (Millipore, 16-663) were added for 2hour at 4°C ...