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Citations for Millipore Sigma :
9851 - 9900 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... 200 ug protein was loaded onto a Microcon Centrifugal Filters (Millipore, Cat: 42407). Filter aided sample preparation (FASP ...
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bioRxiv - Cell Biology 2022Quote: Total protein was extracted by lysing the cells with RIPA Buffer (Sigma-Aldrich) containing phosphatase and protease inhibitors (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... 350 µg total protein concentrated with 3-kDa cut-off centrifugal filter (Millipore) from serum-free medium was subjected to SDS-PAGE with multiple band excision and in-gel digestion ...
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bioRxiv - Cell Biology 2022Quote: Chemical cross-linking of proteins was performed with dithio-bismaleimidoethane (DTME) (Sigma-Aldrich) dissolved in dimethyl sulfoxide (DMSO) ...
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bioRxiv - Immunology 2022Quote: ... GFP-tagged SAMHD1 proteins were immunoprecipitated with anti-SAMHD1 antibody (Sigma-Aldrich, SAB1400478) bound to Protein-A/G-Agarose (ThermoFisher ...
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bioRxiv - Developmental Biology 2022Quote: ... A GST-Drn fusion protein was produced in Origami B (DE3) cells (Novagen) and purified with a glutathione-Sepharose 4B column ...
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bioRxiv - Plant Biology 2022Quote: ... Soluble protein was purified with 200 μl of Glutathione agarose beads slurry (Sigma), gently shaking for 1 hour at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... Protein levels were assessed using immunoblots with HA-HRP (Sigma, Catalog number 12013819001) or FLAG-HRP antibodies (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... 10 µM of indicated proteins were mixed with 100 µM nitrocefin (Calbiochem, Sigma) in a reaction buffer containing EstG storage buffer (50 mM HEPES-NaOH pH 7.2 ...
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bioRxiv - Microbiology 2022Quote: ... Protein fractions were collected from each well and analysed by silver staining (Sigma) and Western blot using monoclonal anti poly histidine alkaline phosphatase antibody (1:20,000 dilution) ...
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bioRxiv - Microbiology 2022Quote: ... was added to 75 μl of prewashed protein A sepharose beads (Sigma-Aldrich) together with 700 μl lysis buffer (20 mM Tris/HCl pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were resolved by SDS-PAGE and transferred onto polyvinylidene difluoride (PVDF; Millipore) membranes ...
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bioRxiv - Microbiology 2022Quote: ... Pure Cas protein was finally concentrated in Amicon Ultra centrifugal filter (Merck-Millipore).
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bioRxiv - Molecular Biology 2022Quote: ... Pellets were transferred to low protein binding tubes (Sigma-Aldrich, Cat# Z666513-100EA) and stored at −80°C
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bioRxiv - Microbiology 2022Quote: ... Proteins were resuspended in 50 mM triethylammonium bicarbonate (TEAB, Sigma cat. no. T7408), reduced for 1 hour at 55°C using 10 mM tris(2-carboxyethyl)phosphine (TCEP ...
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bioRxiv - Neuroscience 2023Quote: ... Residual protein fragments were precipitated with trichloroacetic acid (Sigma-Aldrich, cat. no. T6399). The supernatant ...
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bioRxiv - Microbiology 2022Quote: ... YifLStrep protein was eluted with wash buffer containing 2.5 mM d-Desthiobiotin (Sigma).
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bioRxiv - Cancer Biology 2024Quote: ... followed by protein detection using either Immobilon Western HRP Substrate (WBKLS0500, Geyer/Millipore) or SuperSignal West Femto Maximum Sensitivity Substrate (34095 ...
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bioRxiv - Molecular Biology 2022Quote: ... Equal loading of protein samples was confirmed via Ponceau S staining (Sigma-Aldrich) for 4 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Cleaved proteins were filtered by centrifuging with Ultrafree-MC VV filter (EMD Millipore) in a tabletop centrifuge at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Eluted protein fractions were pooled and concentrated using Amicon 30K Centrifugal filters (Millipore). The concentrated ...
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bioRxiv - Cell Biology 2022Quote: ... Elution fractions containing protein were concentrated using Amicon Ultracel-10K/ Ultracel-30K (Millipore) centrifuge filters and subsequently applied to size exclusion chromatography (SEC ...
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bioRxiv - Cell Biology 2022Quote: ... The expressed GST-fusion protein was absorbed to glutathione-Sepharose 4B (Sigma-Aldrich) followed by extensive washing with phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2024Quote: ... Fractions containing the protein of interest were concentrated (Amicon Ultracel 30 K, Millipore) and further purified by size-exclusion chromatography (SEC ...
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bioRxiv - Biochemistry 2024Quote: ... Immunoreactive proteins were visualized using Immobilon® Forte Western HRP Substrate (Merck Millipore) at the Imager Fluor-Chem M (Proteinsimple) ...
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bioRxiv - Biochemistry 2024Quote: ... the protein was concentrated in a centrifugal filter (Amicon Ultra-15, 10K, Millipore) and washed twice with 15 ml of PBS to remove the imidazole ...
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bioRxiv - Biochemistry 2023Quote: ... The cell lysate was pre-cleared with Protein A agarose beads (Millipore, Sigma), and 700 μg of the pre-cleared lysate was incubated with 2 μg of either anti-CEP41 ...
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bioRxiv - Biochemistry 2023Quote: ... The cell lysate was pre-cleared with Protein A agarose beads (Millipore, Sigma), and 700 μg of the pre-cleared lysate was incubated with 2 μg of either anti-CEP41 ...
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bioRxiv - Biochemistry 2024Quote: ... Protein samples were incubated with 0.2mM methoxypolyethyleneglycol maleimide (NEM-PEG, 5kDa; Sigma-Aldrich) for 1 minute on ice ...
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bioRxiv - Biophysics 2024Quote: ... The dialyzed sample was filtered with a low protein binding filter (Millipore Sigma) and run through a SourceQ15 anion exchange column (Cytiva) ...
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bioRxiv - Cell Biology 2024Quote: ... FLAG-tagged protein was isolated with anti-FLAG M2 magnetic beads (Sigma, M8823) for 2 hr at 4LJ°C and eluted with 100 μg/ml 3x FLAG peptide ...
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bioRxiv - Developmental Biology 2023Quote: ... The lysates were pre-cleared using Protein A agarose beads (11134515001, Sigma-Aldrich) at 4 ℃ for 2 h ...
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bioRxiv - Cell Biology 2024Quote: ... The protein pellet was resuspended in 8M Urea/50mM ammonium bicarbonate (Sigma Aldrich) and centrifuged (12 000 g ...
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bioRxiv - Immunology 2024Quote: ... Eluted proteins were buffer exchanged using 50 kDa Amicon centrifugal units (EMD Millipore), resuspended in phosphate-buffered saline (PBS) ...
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bioRxiv - Neuroscience 2024Quote: ... CDK5 was immunoprecipitated (4°C overnight with 2x Protein A agarose (Sigma-Aldrich)) from SCN samples at ZT14 in the dark or after a light pulse (LP. ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins in the supernatant and pellet were solubilized in 1XLDS sample buffer (Sigma) and analyzed by SDS-PAGE and western blots with anti-CAPS and anti-alpha-tubulin antibodies.
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bioRxiv - Molecular Biology 2024Quote: ... The collected proteins were concentrated by an Amicon Ultra-3k centrifugal filter (Millipore), exchanged with PBS supplemented with 100 mM NaCl and 50% glycerol ...
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bioRxiv - Neuroscience 2024Quote: ... the proteins were incubated with streptavidin-agarose (16-126; Millipore sigma, Burlington, MA) for one night at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... The protein was concentrated using a 10 kDa MWCO Amicon concentrator device (Millipore). The purified proteins and protein standards were subjected to a colorimetric bicinchoninic acid (BCA ...
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bioRxiv - Developmental Biology 2024Quote: ... FLAG-tagged proteins were purified using anti-FLAG M2 Affinity agarose gel (Sigma) for >1h at 4°C ...
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bioRxiv - Immunology 2024Quote: ... The supernatant was passed through low-protein binding 0.45-µm syringe filter (Millipore). The lentivirus was purified by two different methods for comparison ...
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bioRxiv - Microbiology 2024Quote: ... Purified proteins were concentrated using Amicon™ Ultra centrifugal filter units (Millipore Sigma) with a molecular cutoff of 10 kDa and stored at −80 °C.
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bioRxiv - Microbiology 2024Quote: ... The refolded proteins were concentrated with Amicon 3 kDa Ultracel filters (EMD Millipore). For GFP-PSB15 purification ...
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bioRxiv - Microbiology 2023Quote: ... G9R and A1L proteins were labeled with primary anti-Flag (F1804, Sigma-Aldrich) and anti-HA (26183 ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins in the supernatant were digested with 100 µg/mL proteinase K (Sigma) at 37°C overnight ...
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bioRxiv - Microbiology 2024Quote: ... The total protein concentration in the lysates was quantified using Bradford reagent (Sigma), and equal amounts of bacterial proteins were resolved by SDS-PAGE ...
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bioRxiv - Microbiology 2024Quote: ... The total protein content of the samples was quantified using Bradford assay (Sigma). Equal concentrations of protein samples were mixed with 5x Laemmle buffer (Bio-Rad) ...
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bioRxiv - Biochemistry 2024Quote: ... Purified protein samples were concentrated using Amicon Ultra 10K spin column (Sigma-Aldrich) and dialyzed against Storage buffer (25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2024Quote: ... protein was reduced (2.5 mM dithiothreitol, Sigma-Aldrich; 30 min at 37°C) and alkylated (10 mM iodacetamide ...
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bioRxiv - Cancer Biology 2023Quote: ... and 50 µM Rho-associated protein kinase (ROCK) inhibitor (Merck Millipore, SCM-075). On day 3 ...