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Citations for Millipore Sigma :
9801 - 9850 of 10000+ citations for Carbonic Anhydrase 14 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were incubated with rabbit UPF1 antibody (CST #9435) antibody used at 1:1000 dilution in 5% BSA 1X TBST overnight at 4°C or mouse Tubulin (Sigma T9206) used at 1:2000 dilution in 5% milk 1X TBST for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: Primers were designed to span at least one exon-exon junction and to match the target sequence only in mouse (Primer-Blast, NCBI) and were synthesised commercially (Sigma Aldrich). Primer efficiency was determined separately through a dilution series of cDNA samples from wildtype hippocampus ...
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bioRxiv - Neuroscience 2020Quote: Soluble and insoluble human brain samples and mouse brain samples were homogenized in PBS with a protease inhibitor cocktail (catalog no. 11873580001; Sigma Aldrich) with 3.2 mm stainless steel beads ...
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bioRxiv - Biochemistry 2021Quote: ... Coverslip were washed with PBS with 0.25% triton X-100 and followed by incubation with secondary antibodies Alexa Fluor 488 anti-rabbit and Alexa Fluor 594 anti-mouse (1:1000) together with Hoechst 33342 1 µM (14533; Sigma-Aldrich) for nuclei staining during 1h at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... the primary antibody that was used was mouse monoclonal anti-IAV nucleoprotein A1 and A3 blend (EMD Millipore, MAB8251, 1:250). Images were taken at 10x magnification using a Zeiss Axio Observer and Axiocam 503 mono camera (Zeiss).
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bioRxiv - Developmental Biology 2020Quote: ... rabbit anti-GSX2 (1:250 dilution; gift from Kenneth Campbell (Toresson et al., 2000)) mouse anti-Olig2 (1:250 dilution; Millipore (#MABN50), Billerica ...
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bioRxiv - Bioengineering 2021Quote: ... samples were blocked for 1 h in 1% (w/v) bovine serum albumin and incubated with mouse monoclonal anti-vinculin antibody (1:1000, Sigma #V9264) or mouse monoclonal anti-VE-cadherin antibody (1:1000 ...
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bioRxiv - Biochemistry 2020Quote: ... and Anti-Mouse IgG (Fc specific)-FITC antibody produced in goat (Cat # F4143, Sigma Aldrich, USA; 30 min at 4°C).
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bioRxiv - Biochemistry 2020Quote: SV40-immortalized mouse embryonic fibroblasts (MEF, a kind gift of K. L. Rudolph) were grown in high glucose DMEM medium (Sigma, D6429) until they reached 70 % confluency ...
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bioRxiv - Neuroscience 2021Quote: ... followed by overnight incubation at 4 °C with the following primary antibodies diluted in blocking solution: mouse anti-GFAP (1:500, Sigma G3893), and rabbit anti-Iba1 (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated for 1 h at RT with anti-microtubule associated protein 2 (MAP2) mouse monoclonal antibody (diluted 1:200 in PBS/gelatin; Sigma-Aldrich) and with anti-Tau rabbit polyclonal antibody (diluted 1:500 in PBS/gelatin ...
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bioRxiv - Neuroscience 2021Quote: ... They were incubated with the following primary antibodies in blocking solution at 4°C overnight: mouse monoclonal anti-GFAP (1:1000, G3893; Sigma-Aldrich), rabbit polyclonal anti-GFAP (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were incubated overnight at 4°C with a tubulin primary antibody (mouse monoclonal anti-β-tubulin III, 1:500, Sigma) and for 2 h with a CY3-conjugated anti-mouse secondary antibody (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... Rabbit antibody against Parvalbumin (1:500, PA1-933, Thermo Fischer Scientific) and Mouse antibody against GAD67 (1:500, MAB5406, Sigma-Aldrich) in staining buffer (0.2% BSA ...
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bioRxiv - Cell Biology 2021Quote: ... nonfat dry milk in PBS-T for 1 h Membranes were incubated with mouse anti-FLAG M2 antibody (1:500, Sigma-Aldrich) or mouse anti-α-tubulin B512 antibody (1:5000 ...
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bioRxiv - Cancer Biology 2019Quote: ... the membranes were incubated overnight at 4 °C with primary antibodies: mouse monoclonal antibody against α-actinin (1:1,000; Millipore, MAB1682), mouse monoclonal antibody against G6PD (1:250 ...
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bioRxiv - Cell Biology 2021Quote: ... Base media for the culture of mESCs were supplemented with 1000 units/mL ESGRO Recombinant mouse LIF protein (EMD Millipore ESG1107), and 2i small molecule inhibitors ...
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bioRxiv - Neuroscience 2020Quote: ... blocked and permeabilized for 1 h in 10% donkey serum/PBS-T/0.3% triton-X-100 pH 7.4 and incubated for 2 h (RT) in mouse anti-puromycin 12D10 antibody (Sigma Aldrich, MABE343), washed extensively ...
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bioRxiv - Physiology 2021Quote: ... Protein detection was performed by the use of the HRP-conjugated secondary antibodies: anti-rabbit (CST #7074) or anti-mouse (CST #7076) and Immobilon Forte Western HRP substrate (Millipore Sigma). Protein band intensity on exposed film was measured with Adobe Photoshop software.
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bioRxiv - Biochemistry 2021Quote: ... chromatin isolated from 1–2 × 106 cells were incubated with 5 µg of mouse anti-FLAG (F1804, SIGMA-ALDRICH - RRID: AB262044) antibody pre-coupled to Protein A/G Sepharose ...
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bioRxiv - Biochemistry 2021Quote: Anti-FLAG immunoprecipitation was performed using 20 μL of packed agarose-conjugated mouse anti-FLAG antibody (M2 Affinity gel, A2220 Sigma-Aldrich) and were targeted either against the exogenously expressed transgenes (HMR+ ...
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bioRxiv - Biochemistry 2021Quote: ... mouse APC-conjugated antibody directed against dsRNA J2 (IF 1:200, Scicons, 10010500) mouse anti-3xFlag M2 (WB 1:25000, Sigma, F1804), rabbit anti-Tubulin (WB 1:4000 ...
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bioRxiv - Biochemistry 2020Quote: ... Chemiluminescence of the appropriate secondary rabbit or mouse HRP-conjugated antibodies after incubation with ECL HRP substrate (Immobilon™ Western HRP Substrate, Millipore) was detected using a Chemi Doc™ Quantity One® system (Bio-Rad).
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bioRxiv - Neuroscience 2020Quote: Free floating mouse brain sections were incubated with 300 nanograms of active human cathepsin-S (SRP0292, Sigma-Aldrich, St. Louis, MO), in activation buffer containing 1.8 mM DTT ...
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bioRxiv - Systems Biology 2020Quote: ... Bait proteins were probed using mouse anti-FLAG antibody (Monoclonal anti-FLAG M2 antibody, Sigma-Aldrich, F3165; used at 1:2500) in blocking buffer ...
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bioRxiv - Microbiology 2020Quote: ... were used with secondary anti-mouse IgG or anti-rabbit IgG conjugated with horseradish peroxidase antibodies (A9917 and AP132P, Sigma Aldrich). SuperSignal West Pico Chemiluminescent substrate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were incubated with 1:10,000 vol vol−1 secondary antibody anti-mouse horseradish peroxidase (HRP) conjugate (A5278, Sigma–Aldrich, USA) in 2% weight vol−1 BSA-TTBS ...
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated with blocking solution (1% bovine serum albumin and 0.2% Triton-X in 0.01 m PBS) for 1 hour at room temperature before primary antibodies were applied in blocking solution [mouse anti-parvalbumin antibody (Millipore, MAB1572) at 1:2000 overnight ...
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bioRxiv - Systems Biology 2021Quote: ... and 8 FOVs in replicate 3 of the mouse cortex) were registered using nuclei signals stained with DAPI solution consisting of 5 μg/mL DAPI (Sigma D8417) and 4× SSC ...
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bioRxiv - Genetics 2021Quote: ... Collected blastocysts were cultured on mitomycin-treated mouse embryonic fibroblast feeder cells in 2i-LIF medium (ESGRO Complete Basal medium (Millipore, Germany) supplemented with leukemia inhibitory factor (Wako ...
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bioRxiv - Microbiology 2021Quote: ... and stained with sACE2-humanFc fusion protein (construct is a gift of Jason McLellan, University of Texas at Austin) or mouse anti-Flag antibody (Sigma, F3165). Cells were then stained with anti-human-IgG-FITC (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated overnight at 4 °C with the respective primary antibodies (rat-monoclonal anti- BrdU −1:200- Abcam; Rabbit polyclonal antibody anti-DCX,1:500- Cell Signaling; mouse monoclonal anti-NeuN- Millipore) diluted in a 0.1 % BSA and 0.125 % Triton X-100 solution ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated with primary antibodies (rabbit anti-UCP1, 1:750, Abcam ab10983, mouse anti-tyrosine hydroxylase, 1:1,500, Sigma-Aldrich T1299) in blocking buffer overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: Mouse phospho-MLKL (Ser345) rabbit monoclonal (D6E3G) (Cell Signalling Technology) MLKL rat monoclonal 3H1 (available from Millipore MABC604; generated in-house) ß-actin mouse monoclonal AC-15 (Sigma A-1978)
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bioRxiv - Microbiology 2021Quote: ... were immunized in a prime-boost-boost regimen with post-fusion hMPV B2 F protein (50 µg protein/mouse) in a water in oil emulsion with Titermax Gold adjuvant (Sigma-Aldrich) via subcutaneous (SC ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were washed and incubated for 1 h at room temperature with primary antibody (anti-HA, mouse, 1:500, Sigma-Aldrich) diluted in PBS containing 1 % bovine serum albumin ...
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bioRxiv - Molecular Biology 2021Quote: ... Expression of the dCasRX-YTHDC1 fusion protein was confirmed by transfection of the plasmid followed by Western Blot with anti-FLAG M2 mouse monoclonal antibody (F1804, Sigma-Aldrich) and anti-YTHDC1 (14392-1-AP ...
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bioRxiv - Microbiology 2020Quote: ... mouse polyclonal antibody was produced immunizing a mouse with 50 mg of purified recombinant MtEPSPS containing Freund’s incomplete adjuvant (Sigma-Aldrich, USA) (total volume of 100 µL ...
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bioRxiv - Neuroscience 2021Quote: ... for 1hour at RT and then incubated overnight at 4°C with a mouse anti-βIII-Tubulin (TUJ1, Sigma, 1/500) and a rabbit anti-ISLET1 (Abcam ...
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bioRxiv - Cell Biology 2021Quote: ... the membranes were incubated with HRP-conjugated secondary antibody (1:10000; anti-rabbit, Sigma-Aldrich A0545; anti-mouse, Sigma-Aldrich A0168) in TBST with 1% milk ...
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bioRxiv - Cell Biology 2021Quote: ... the membranes were incubated with HRP-conjugated secondary antibody (1:10000; anti-rabbit, Sigma-Aldrich A0545; anti-mouse, Sigma-Aldrich A0168) in TBST with 1% milk ...
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bioRxiv - Cell Biology 2021Quote: ... Staining reagents: anti-tubulin (clone DM1A, mouse, 1:1000, Sigma; alternatively, clone YL1/2, rat, 1:500, Millipore Bioscience Research Reagents); anti-DmEb1 (gift from H ...
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bioRxiv - Cell Biology 2021Quote: ... 80 µg protein were loaded into a 10 % SDS gel and stained with anti-Tau (clone Tau46, T9450, mouse, 1:1000, Sigma-Aldrich).
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Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survivalbioRxiv - Cell Biology 2021Quote: ... TA muscles were carefully removed and then frozen in liquid nitrogen and stored (−80 °C) for immunoblotting analysis using a mouse IgG2a monoclonal anti-puromycin antibody (clone 12D10, 1:2500; Millipore # MABE343).
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bioRxiv - Immunology 2021Quote: ... mice were randomly assigned to receive intraperitoneal injections of either Vehicle (PBS) or 5mg/kg/mouse Ganciclovir (GCV, Sigma cat#G2536) for the next 3 weeks while still on WD ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were fixed with 2% paraformaldehyde in PBS and proximity ligation assays (PLA) were performed with the Duolink In Situ Red Starter Kit Mouse/Rabbit (Sigma Aldrich) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2020Quote: ... Every 9th free-floating section was selected and stained with mouse anti-NeuN antibody (EMD Millipore, Billerica, MA, USA; 1:1000), which is a neuronal marker ...
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bioRxiv - Biophysics 2020Quote: ... A second incubation of 1 hour at room temperature was then performed with goat anti mouse Alexa Fluor 555 and anti-rabbit FITC conjugated (1:400, F7512, Sigma Aldrich). For all the labelling ...
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bioRxiv - Biophysics 2020Quote: ... permeabilized cells were blocked with 3 % BSA in PBS for 30 min at room temperature and then incubated with mouse monoclonal anti-alpha-tubulin antibody (T6199, clone DM1A, Sigma-Aldrich) diluted at 1:200 in 3 % (w/v ...
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bioRxiv - Biophysics 2020Quote: ... Primary antibody staining was carried out by placing coverslips upside down onto a drop of primary antibody staining mix (mouse-anti-beta-tubulin, catalog no. T5293, Sigma-Aldrich, diluted 1:300 in PBS containing 2% (w/v ...