Labshake search
Citations for Millipore Sigma :
9551 - 9600 of 10000+ citations for Carbonic Anhydrase 14 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2022Quote: ... The primary antibodies used were as follows: mouse anti-phospho-Histone H2A.X (Ser139; clone JBW301; Sigma, 05-636; 1/1,000), mouse tubulin (clone B-5-1-2 ...
-
bioRxiv - Biochemistry 2022Quote: ... Flag-tagged H2B was detected with western blot using mouse anti-FLAG (F3165, Sigma-Aldrich, RRID: AB_259529, 1:3000 diluted) and HRP-conjugated anti-mouse (sc-516102 ...
-
bioRxiv - Cancer Biology 2022Quote: ... diluted 1:1000 in casein and incubated overnight at 4 degrees Celsius with shaking OR Monoclonal Anti-Beta-Actin mouse antibody (Sigma) diluted 1:5000 in casein and incubated at room temperature for 1 hour ...
-
bioRxiv - Biophysics 2022Quote: ... the acini were incubated with the primary antibody mouse anti-podocalyxin (EMD Millipore, clone 3F2:D8, working dilution 1:200), mouse β1 integrin (Invitrogen ...
-
bioRxiv - Biophysics 2022Quote: ... The pre-cleared lysates were then incubated with 100 µL of antibody/protein A complexed beads (anti-FLAG [mouse, Sigma, F1804] or anti-HA [mouse ...
-
bioRxiv - Cell Biology 2022Quote: ... U2OS and SYF (src -/- yes -/- fyn -/-) mouse embryonic fibroblasts (Klinghoffer et al., 1999) were cultured at 37 °C with 5% CO2 in DMEM (Sigma) with 4.5 g/l L-glucose ...
-
bioRxiv - Cell Biology 2022Quote: Proximity ligation assay (PLA (53)) was performed using the In Situ Red Starter Kit Mouse/Rabbit (Duolink DUO92101, Sigma Aldrich). The conditions specified by the manufacturer’s instructions were optimized by incubating sections with primary antibodies overnight at 4℃ and increasing incubation time with probes and amplification reagents to 2 hours as previously described (54) ...
-
bioRxiv - Cell Biology 2022Quote: ... The mouse monoclonal anti- FLAG (catalog #: F1804) and anti-β-actin (catalog #: A1978) antibodies came from Sigma (St. Louis, MO). The mouse monoclonal anti-Hsp47 antibody (catalog # ...
-
bioRxiv - Cell Biology 2022Quote: The following monoclonal antibodies were used for immunofluorescence: anti-acetylated tubulin (mouse; clone 6-11B-1; Sigma-Aldrich; 1:500), anti-ubiquitin clone FK2 (mouse ...
-
bioRxiv - Genetics 2022Quote: ... Binding of the anti-FLAG M2 primary antibody (mouse, Sigma-Aldrich, F3165, diluted 1:3000 in PBS-T+1%BSA) was allowed in a humidity chamber (overnight ...
-
bioRxiv - Developmental Biology 2022Quote: ... Naive hPSCs were grown either on irradiated MF1 mouse embryonic fibroblasts (MEFs) (Wellcome-MRC Cambridge Stem Cell Institute) on plates pre-coated with 0.1 %Gelatin (Sigma-Aldrich), or in feeder-free conditions using Geltrex Matrix (ThermoFisher Scientific ...
-
bioRxiv - Neuroscience 2022Quote: ... Detection was carried out using anti-mouse IgG-HRP antibody (GE; 1:5000 in PBS + 2% BSA) followed by addition of TMB substrate (Sigma). Reaction was stopped by adding 0.5 M H2SO4 ...
-
bioRxiv - Microbiology 2022Quote: ex-vivo mouse fecal experiments with sterile and non-sterile mouse fecal media were conducted aerobically at 37°C in 48 well plates (Sigma) with shaking (210 rpms) ...
-
bioRxiv - Microbiology 2022Quote: ... the plates were washed 3 times with 1x PBS to remove the supernatant and the degradation of the pre-coated ECM components was detected using mouse anti-fibronectin (F7387, 1:5,000; Millipore Sigma), and rabbit anti-laminin (L9393 ...
-
bioRxiv - Microbiology 2022Quote: ... samples were washed with 1 ml PBS buffer three times and vacuum-infiltrated for 20 min with 0.1 mg/ml of primary mouse anti-1,3-β-glucan antibody (Biosupplies; Sydney, Australia) for β-1,3-glucan labelling or 0.1 mg/ml mouse MOPC-104E primary antibody (Sigma-Aldrich) for α-1,3-glucan labelling ...
-
bioRxiv - Pathology 2022Quote: ... The membranes were washed three times with Tris-buffered saline and Tween-20 followed by incubation with the peroxidase-conjugated anti-mouse or anti-rabbit secondary antibodies (both from Millipore) for 1 h at room temperature.
-
bioRxiv - Neuroscience 2022Quote: ... Binding of the FLAG-tagged DARPins to streptavidin-immobilized biotinylated gephyrin variants was measured using a mouse-anti-FLAG-M2 antibody (Sigma) as 1st and goat-anti-mouse-alkaline phosphatase conjugated antibody (Sigma ...
-
bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were washed with TBS-T and membranes were incubated with secondary antibodies anti-mouse and anti-rabbit coupled to alkaline phosphatase (1:5000; Sigma) diluted in TBS-T/1% BSA for one hour at RT ...
-
bioRxiv - Microbiology 2022Quote: ... RBD or spike-bound serum antibodies were detected with horseradish peroxidase conjugated goat anti-mouse IgG (1:500 dilution, Milipore Sigma) incubating for 2 h at 25°C ...
-
bioRxiv - Neuroscience 2023Quote: ... we randomly assigned Ai9:Tmem119cre mice to receive an intravenous injection of control solution or 0.05 mg/kg of recombinant IFNγ from mouse (Sigma #I4777), minutes before initiating the rupture of cortical capillaries and then 12h later ...
-
bioRxiv - Molecular Biology 2023Quote: ... HRP-conjugated anti-rabbit (catalog no. 401315) and anti-mouse (catalog no. 401215) secondary antibodies and BSA (catalog no. 12659) were from Millipore. All antibodies were diluted in 1% BSA dissolved in PBS-T (1.5 mM Sodium Phosphate Monobasic ...
-
bioRxiv - Physiology 2023Quote: ... a colorimetric-based enzyme assay was used to measure alanine aminotransferase (ALT) levels in peripheral plasma collected from the submandibular vein monthly using a Mouse ALT Activity Assay Kit (SIGMA). Serum LBP level was measured by a Mouse Lipopolysaccharide Binding Protein ELISA Kit (HyCult Biotechnology ...
-
bioRxiv - Physiology 2023Quote: One kidney from each mouse was mechanically dissociated using scissors and digested with 0.156mg/ml Collagenase type XI (Sigma-Aldrich), 0.030mg/ml Hyaluronidase type IV-S (Sigma-Aldrich) ...
-
bioRxiv - Immunology 2022Quote: ... primary antibodies were incubated overnight at 4°C: mouse monoclonal anti-C5aR1 (clone: S5/1; Millipore; cat. MABF1980; 1:50), rabbit polyclonal anti-IBA1 (FUJIFILM Wako Pure Chemical Corporation ...
-
bioRxiv - Neuroscience 2022Quote: ... The membranes were blocked at room temperature for 1hr in 4% skimmed milk in TBS (0.1% tween in PBS) and probed with 1:1000 diluted mouse anti-FLAG (Sigma-Aldrich). Anti-mouse secondary antibodies conjugated to horseradish peroxide (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2022Quote: ... To assess the NOTCH3 ECD deposits the tissues were incubated overnight at 4 °C with mouse monoclonal anti-human NOTCH3 ECD primary antibody (1:100, clone 1E4, Millipore) followed by detection with Alexa 594 conjugated anti-mouse secondary antibody (1:1000 ...
-
bioRxiv - Neuroscience 2022Quote: ... Washing steps were performed using PBS-Tween buffer followed by horseradish peroxidase-conjugated goat anti-mouse IgG antibody (Sigma-Aldrich) for 30 minutes at room temperature ...
-
bioRxiv - Molecular Biology 2022Quote: ... followed by probing with respective goat anti-mouse or rabbit secondary antibodies conjugated with alkaline phosphatase (dilution 1: 5,000; Sigma-Aldrich) or conjugated with HRP (dilution 1:2000 ...
-
bioRxiv - Molecular Biology 2022Quote: ... The following antibodies were used for ChIP analysis: Mouse anti-RNA polymerase II antibody clone CTD4H8 (Merck Millipore, 05-623), Rabbit anti-NF-kB p65 antibody clone D14E12 (Cell Signalling ...
-
bioRxiv - Neuroscience 2022Quote: ... α-tubulin antibody was used as loading control (mouse anti-α-tubulin, 1:10000; Sigma-Aldrich cat#T6074, RRID: AB_477582). Antibodies were functionally validated by Cell Signalling Technology and Sigma-Aldrich companies ...
-
bioRxiv - Neuroscience 2022Quote: ... injected regions (left frontal cortex) or the control region (right frontal cortex) of mouse brains were prepared in RIAPA buffer (R0278, Sigma) with protease and phosphatase inhibitor ...
-
bioRxiv - Molecular Biology 2022Quote: ... Golgi apparatus was detected with mouse anti-Golgi 58K protein /formiminotransferase cyclodeaminase (FTCD) antibody (G2404, SIGMA-Aldrich, Prague, Czech Republic). The following secondary antibodies were used for detection ...
-
bioRxiv - Cancer Biology 2022Quote: ... AD5-CC10-Cre or 109 PFU Ad5-SPC-Cre (Viral Vector Core Facility, University of Iowa, USA) per mouse in 60 ml total volume of medium (MEM, Sigma) with 9.67 mM CaCl2 ...
-
bioRxiv - Cell Biology 2022Quote: ... Dylight 550-labeled rabbit anti-BUB-1 (custom made, 74) and FITC-labeled mouse anti-α-tubulin (SIGMA F2168, DM1α) were used at a concentration of 1 μg/mL ...
-
bioRxiv - Plant Biology 2024Quote: ... with Peroxidase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) (115-035-003, Jackson) or Peroxidase Conjugated Goat Anti-Rabbit IgG (H+L) (AP132P, Sigma) as secondary antibodies ...
-
bioRxiv - Plant Biology 2024Quote: ... and subjected to immunoblot analysis with the relevant primary and secondary antibodies (anti HA rabbit polyclonal, Abcam ab20084; anti AV2 CP rabbit polyclonal, DSMZ RT-0953-0645/1; anti c-Myc mouse monoclonal, Sigma M4439 ...
-
bioRxiv - Neuroscience 2024Quote: ... Fresh mouse astrocytes were added to the culture by means of hanging cell culture inserts (MCRP06H48, Millipore, transwell for clarity) on day 4 after definitive plating of neurons in poly-d-lysine-coated 6 well plates or added into poly-L-ornithine-coated glass coverslips treated with nitric acid for IF experiments at a ratio 1:1 (human neuron to mouse astrocyte).
-
Activation of XBP1s attenuates disease severity in models of proteotoxic Charcot-Marie-Tooth type 1BbioRxiv - Neuroscience 2024Quote: ... Peroxidase-conjugated secondary antibodies (anti-rabbit HRP, DAKO, P0448; anti-chicken IgG-peroxidase, Sigma; anti-mouse IgG peroxidase, A3682, Sigma) were visualized using Amersham ECL or ECL Prime 225 reagent (GE Healthcare ...
-
bioRxiv - Cell Biology 2023Quote: ... hippocampi were dissected from P2 mouse pups in cold Hanks’ balanced salt solution (HBSS) supplemented with 0.08% D-glucose (Sigma-Aldrich), 0.17% Hepes ...
-
bioRxiv - Physiology 2024Quote: ... The following primary antibodies were used: Rabbit anti-Orai1 (1:50, from Dr. Stefan Feske) and mouse anti-ORAI1 (1:50, SAB3500126, SIGMA), rabbit anti-STIM1 (1:200 ...
-
bioRxiv - Cell Biology 2023Quote: ... Coverslips were blocked in 3% BSA in PBS plus 0.4% Triton X-100 for 30 min at RT and incubated in primary antibody mouse anti-laminA (Sigma Aldrich) at 1:500 overnight at 4 °C ...
-
bioRxiv - Physiology 2024Quote: ... Serum samples were stored at −80 °C until assessed by ELISA according to manufacturer’s instructions (Ultrasensitive Human Growth Hormone ELISA, 22-HGHHUU-E01, ALPCO; mGH, EZRMGH-45K and Rat/Mouse Insulin ELISA, EZRMI-13K, Millipore).
-
bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated for 48 h in the primary antibodies: Monoclonal mouse anti-parvalbumin (1:5000; P3088; Sigma-Aldrich) and Wisteria floribunda lectin (1:500 ...
-
bioRxiv - Cell Biology 2024Quote: The following antibodies were used for immunohistochemistry and western blots: mouse anti-tubulin clone B-5-1-2(Millipore Sigma), rabbit anti-CAPS (Abcam EPR15631) ...
-
bioRxiv - Developmental Biology 2024Quote: ... Embryos were then washed briefly and incubated at 37°C for 30 min in 20% anti-mouse serum antibody (Sigma) or 20% anti-human serum antibody (Sigma) ...
-
bioRxiv - Microbiology 2023Quote: ... 21 and 42 with 50 μg of recombinant LMW630 mixed with 200 ng/mouse pertussis toxin (Sigma-Aldrich, MO, USA). Enzyme-linked immunosorbent assay was performed to measure serum responses to antigen (see methods below ...
-
bioRxiv - Molecular Biology 2024Quote: Primary antibodies used in this study were: mouse anti-FLAG M2 (Sigma-Aldrich #F3165, RRID: AB_259529, 1:1000-1:2500), mouse anti-α-tubulin (Cell Signaling Technologies #3873 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Further confirmation of purification of the recombinant protein was obtained by immunoblots probed with mouse anti-polyhistidine IgG (Sigma-Aldrich) and goat anti-mouse alkaline phosphatase-conjugated antibodies (Thermo Fisher Scientific) ...
-
bioRxiv - Molecular Biology 2024Quote: ... 0.1% Tween-20 (TBS-T) and incubated for one hour at RT with Mouse anti-FLAG (M2) antibody-HRP conjugated (Sigma) diluted 1:4000 in blocking milk ...
-
bioRxiv - Molecular Biology 2024Quote: ... the BG4 antibody67 was incubated for 1 hour at 37°C and subsequently incubated with 1:800 mouse anti-FLAG antibody (F1804, Sigma) to BG4 and 1:500 rabbit anti-PARP1 (ab227244 ...