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Citations for Millipore Sigma :
9451 - 9500 of 10000+ citations for Carbonic Anhydrase 14 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... Primary antibody was washed three times with PBS for 15 min and subsequently incubated with 1:40 Alexa 594 - conjugated anti-mouse rabbit monoclonal antibody (Sigma) in PBS at room temperature for 1 h ...
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bioRxiv - Genomics 2019Quote: ... we blocked coverslips with 5% BSA in PBT for 1 hour at room temperature and then incubated coverslips with properly diluted primary antibody (mouse M2 monoclonal ANTI FLAG, 1:800, F1804, Sigma; rabbit polyclonal Tom20 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... membranes were blocked for 1 h at RT in 5% skimmed milk powder and 0.2% Tween in PBS before incubating overnight at 4◦C with the primary antibodies mouse anti-TH (1:1000, Millipore) and rabbit anti-GAPDH (1:1000 ...
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bioRxiv - Cell Biology 2019Quote: ... Coverslips were blocked for 30 min in 4% Bovine Serum Albumin then incubated overnight with a mouse anti-puromycin monoclonal antibody (Millipore) (1:1000 in 4% BSA) ...
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bioRxiv - Cell Biology 2019Quote: ... and seeded in a concentration of 1.6 × 105 cells/cm2 (passage 39) on mouse laminin (5 µg/cm2, Sigma-Aldrich) coated 0.4 μm pore polyester membrane inserts (Corning) ...
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bioRxiv - Developmental Biology 2020Quote: ... The trophectoderm was removed by immunosurgery and “epiblasts” were grown in gelatinised dishes in the presence of NDiff + 2i and ESGRO (recombinant mouse LIF Protein, Millipore) to establish ESC lines.
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bioRxiv - Synthetic Biology 2020Quote: ... and incubated for 1 h at room temperature or overnight at 4°C in primary antibody (Mouse-anti-FLAG M2 [Sigma #F1804 ...
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bioRxiv - Genetics 2020Quote: ... Cells were incubated overnight at room temperature in blocking buffer containing primary antibody (mouse anti-UBE3A (Sigma SAB1404508 clone 3E5)) at 1:250) ...
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bioRxiv - Cell Biology 2019Quote: ... mES cells were cultured either on a monolayer of gamma irradiated mouse embryonic fibroblasts (IRR-MEFs; StemGent) or gelatin coated dishes (Sigma). Fused monolayers of NIH/3T3 cells and bFFs were cultured in fibroblast medium ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were allowed to recover for 36 hours after media change and Nuclear-Complex Co-IP was performed using commercially available Nuclear-Complex Co-IP kit from ActiveMotif(54001) with manufacturer’s recommended protocol using mouse Anti-Flag (Sigma F3165) or mouse Anti-His (Abcam 18184 ...
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bioRxiv - Pathology 2020Quote: Mouse primary hepatocytes were isolated by gradient density centrifugation of 30% Percoll solution after liver perfusion using Collagenase II (Sigma). Primary hepatocytes were cultured in William’s E Medium (Thermo Fisher Scientific ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... The rehydrated slides were blocked with 2% horse-serum followed by incubation with primary antibodies (Mouse anti-S-glycoprotein antibody, 1:200 dilution, Millipore, Cat # MAB5676 ...
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bioRxiv - Pathology 2020Quote: ... IP was performed by incubation of the resulting lysates overnight at 4 °C with mouse monoclonal anti-Flag antibody (Cat.# F3165, Sigma) or with normal mouse IgG (Cat.#A7028 ...
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bioRxiv - Immunology 2020Quote: ... the membranes were incubated with the appropriate horseradish-peroxidase conjugated secondary Ab (1:5000 in TBST + 2% BSA; 2 h at room temperature; anti-mouse A4416 and anti-rabbit A0545, Sigma). The immunoreactivity was detected by ECL reagents (Amersham) ...
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bioRxiv - Developmental Biology 2019Quote: Protein lysates of tissue isolated from the tail end of mouse embryos at E10.5 were investigated with Rabbit anti-SNX14 (Sigma, HPA017639) as previously reported (9).
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bioRxiv - Developmental Biology 2020Quote: ... mouse monoclonal anti-Tau-1 (05-838, 1:1000), mouse monoclonal anti-MAP2 (05-346, 1:500), rat monoclonal anti-tubulin (MAB1864, 1:500) from Millipore; chicken polyclonal anti-GFP (ab13970 ...
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bioRxiv - Developmental Biology 2021Quote: ... were grown on 0.1% gelatin-coated flasks in 8% CO2 at 37C and cultured in mouse ES media serum+2i+LIF: DMEM (Sigma) without phenol-red ...
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bioRxiv - Molecular Biology 2020Quote: ... The secondary antibodies used in this study were as follows: anti-mouse IgG whole molecule-Peroxidase (1:80,000; catalog no. A9044, Sigma-Aldrich) and anti-rabbit IgG whole molecule-Peroxidase (1:80,000 ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were further incubated for two nights at 4°C with mouse ACTA2-FITC antibodies (1:200; Sigma, F3777) in blocking buffer containing 1-5% bovine serum albumin in 0.25 – 0.5% Triton-X 100 in 1× PBS ...
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bioRxiv - Neuroscience 2021Quote: ... mouse monoclonal anti-synaptophysin (1:500; SMC-178D, StressMarq Bio.) and rabbit monoclonal anti-GluR1 (1:500; 04-855, Millipore). Secondary antibodies were Alexa Fluor 488-donkey anti-mouse IgG (1:300 ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were incubated free-floating overnight at RT with the mouse G10 anti-reelin primary antibody (1:3000, MAB5406; Millipore) diluted in the blocking solution ...
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bioRxiv - Neuroscience 2021Quote: Immunohistochemical staining was performed on free-floating brain sections using the following primary antibodies: mouse anti-rat TH (1:4000, MAB318, Merck Millipore), rabbit anti-Girk2 (1:500 ...
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bioRxiv - Physiology 2020Quote: ... the membrane was incubated with rabbit anti-Urn8R (1:1000) and mouse anti-tubulin (1:2500; Sigma-Alrich, MO, USA) in blocking buffer supplemented with 0.2% Tween 20 (w/v) ...
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bioRxiv - Developmental Biology 2020Quote: ... Sections were then blocked with 5% normal donkey serum in 1X PBS for one hour before overnight incubation in primary antibodies (Foxf1, goat, R&D, 1:300; Sox9, mouse, Invitrogen, 1:200; Sox9, rabbit, Millipore, 1:200 ...
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bioRxiv - Immunology 2021Quote: ... blocked in 5% BSA for 30 min and incubated ON with mouse anti-GAD67 monoclonal antibody (1:6000, MAB5406, Merck Millipore) or 1 h with rabbit polyclonal anti-GAPDH antibody (1:3000 ...
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bioRxiv - Molecular Biology 2021Quote: ... Zygotes were harvested from oviducts at 28 h after hCG injection and then cultured in KSOM Mouse Embryo Media (Millipore) at 37°C with 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... Blots were incubated with goat anti-mouse horse radish peroxidase (HRP)-conjugated secondary antibody and the bands developed with Luminata Forte Western HRP Substrate (Merck Millipore).
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bioRxiv - Microbiology 2021Quote: ... Sera were incubated for one hour at room temperature followed by three washes with PBST before incubating with an anti-mouse IgG secondary antibody conjugated to alkaline phosphatase (Sigma) used as a 1:5000 dilution for one hour ...
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bioRxiv - Microbiology 2021Quote: Isotyping of the monoclonal antibodies and polyclonal sera responses was performed using the Mouse Monoclonal Antibody Isotyping Kit (Sigma-Aldrich), according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... Expression of GAPDH was used as a loading control using a mouse anti-GAPDH antibody (1:10000 dilution, MAB374, Sigma) and IRDye 680CW goat anti-mouse IgG secondary antibody (1:5000 dilution ...
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bioRxiv - Pathology 2021Quote: ... postmortem kidney was fixed in 20% formalin for 2 weeks and processed for free-?oating preparation as done for mouse tissue with one exception that anti-human CD31 antibody (CBL468, 1:20 dilution, Millipore) was used.
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bioRxiv - Systems Biology 2020Quote: ... Immunoprecipitation was carried out by incubating the lysate with the monoclonal anti-FLAG M2-mouse primary antibody (Sigma Aldrich, F1804) and protein G Dynabead (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... The following primary antibodies were used for the Western blotting: mouse antibodies against Flag (Sigma-Aldrich, St. Louis, MO, USA) and mouse antibody against β-actin (Proteintech Group ...
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bioRxiv - Cell Biology 2020Quote: ... and the mouse monoclonal and rabbit polyclonal anti-phosphorylated histone H3 (Ser10) antibodies (ref. 06570, 1:1000) were obtained from Millipore. The anti-GFP mouse monoclonal antibody mix (ref ...
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bioRxiv - Synthetic Biology 2020Quote: ... The membrane was then washed several times with Tris-Buffered Saline with 0.1% Tween 20 (TBST) before a 30 in incubation with monoclonal Mouse Anti-FLAG M2-Peroxidase (HRP) antibodies (Sigma-Aldrich) diluted 1:5000 in TBST ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for 24 hours at 4 °C in mouse anti-GAD67 (1:1000; Millipore Sigma, cat# MAB5406), rabbit anti-NeuN (1:500 ...
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bioRxiv - Plant Biology 2021Quote: ... GSTU5 was detected by the anti-GST primary antibody (Novex, 1:500) and anti-mouse secondary antibody (Sigma, 1:5000).
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bioRxiv - Immunology 2020Quote: ... The pLKO vectors either encoded two specific shRNAs against mouse Akt1 (Cat# TRCN0000304683) or a non-silencing control shRNA sequence (Cat# SHC002) (all from Sigma). Akt1 shRNA and control non-silencing shRNA viruses were intratracheally instilled in mice in a volume of 50 µl ...
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bioRxiv - Immunology 2019Quote: ... 0.1% [w/v] saponin in PBS) for 25 min at RT and then stained with mouse anti-flavivirus antibody (clone D1-4G2-4-15, Millipore) diluted 1:500 in FACS wash buffer for 90 min at RT ...
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bioRxiv - Plant Biology 2019Quote: ... the blot was washed with 1×TBS-T solution (4×5min) and incubated with anti-Mouse IgG-HRP secondary antibody solution (Sigma, 1:10,000 dilution in 1×TBS-T with 0.2% Blocking Reagent [Roche] ...
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bioRxiv - Developmental Biology 2020Quote: HRP-conjugated secondary antibodies for Western blotting: goat anti-mouse IgG (Cat# A 4416, Sigma-Aldrich, St. Louis, MO, USA) and goat anti-rabbit IgG (Cat# GtxRb-003-EHRPX ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were resolved in SDS-PAGE gels and transferred to nitrocellulose membranes that were incubated overnight at 4 °C with one of the following antibodies: mouse monoclonal anti-HA (1:2000, Sigma); rabbit polyclonal MBP-probe (1:1000 ...
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bioRxiv - Microbiology 2019Quote: ... Plates were washed twice with PBS and incubated in same conditions with horseradish peroxidase (HRP)-conjugated goat anti-mouse secondary antibody (Sigma), diluted 1:1000 in blocking buffer ...
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bioRxiv - Biochemistry 2020Quote: ... The Flag-tagged receptor was detected using mouse anti-Flag M2 primary antibody (1:10,000 dilution; Sigma Aldrich, catalog #F3165) and IRDye® 680RD-conjugated donkey anti-mouse IgG secondary antibody (1:20,000 dilution ...
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bioRxiv - Microbiology 2019Quote: ... The cells were then treated with 5% BSA blocking solution for 1 h and the incubated with mouse monoclonal anti-HA (Sigma) antibody and rabbit anti-Flag antibody (Sigma ...
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bioRxiv - Immunology 2019Quote: ... Mouse neutrophil was isolated from the peritoneal cavity 3 hours after injection of 1 ml thioglycollate medium (Sigma-Aldrich, MO). Isolated neutrophils were incubated at 37C for 6 hours and then treated with 10 ng/ml ...
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bioRxiv - Physiology 2020Quote: ... and probed overnight (4 °C) with one of the following primary antibodies: Anti-TSG-6 mouse monoclonal (1 μg/ml; Millipore); rat monoclonal TNFα (1 μg/ml ...
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bioRxiv - Neuroscience 2019Quote: ... and grown in standard human embryonic stem (ES) cells media supplemented with 6ng/ml basic Fibroblast Growth Factor (bFGF) on inactivated mouse embryonic fibroblast (MEFs) (Sigma) as feeder cells ...
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bioRxiv - Bioengineering 2019Quote: For Flag-tag detection membranes were incubated for two hours with the monoclonal anti-Flag M2 antibody from mouse (F3165, Sigma) in a 1:5000 dilution (in TBS-T ...
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bioRxiv - Physiology 2019Quote: Mouse islets were isolated as previously described61 using Type XI collagenase (1000 units/mL; #C7657, Sigma-Aldrich, St. Louis, MO). Following digestion and filtration ...