Labshake search
Citations for Millipore Sigma :
9651 - 9700 of 10000+ citations for Carbonic Anhydrase 14 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2022Quote: ... were cultured in the presence of recombinant mouse IL-4 (25 ng/mL; #404-ML, R&D) and LPS (20 μg/mL; #L2880, Sigma) over night to induce B cell activation and terminal differentiation ...
-
bioRxiv - Cell Biology 2023Quote: ... mESCs were grown at 37°C and 5% CO2 on Mitomycin-C inactivated feeder cells (DR4-mouse embryonic fibroblast) plated on 0.1% gelatin (Sigma-Aldrich) in serum/leukemia inhibitory factor (LIF ...
-
bioRxiv - Cancer Biology 2023Quote: The mouse E0771 (derived from a spontaneous triple negative mammary tumor that arose in a C57BL/6 mouse) and the human K562 (originally generated from a female leukemia patient) cell lines were cultured in RPMI-1640 (Sigma) supplemented with 10% FCS (GIBCO) ...
-
bioRxiv - Genetics 2022Quote: ... for CLK and BMAL1 expression then the secondary antibody of horseradish peroxidase-conjugated either anti-mouse or anti-rabbit IgG antibody (Sigma). Chemiluminescence detection was performed by using ECL Western Blotting detection Reagent (GE Healthcare) ...
-
bioRxiv - Developmental Biology 2023Quote: ... The samples were incubated overnight in a humid chamber at 4°C with the following primary antibodies: mouse anti-pan polyglycylated tubulin (1:1000, Axo-49; Sigma) and anti-cleaved caspase 3 (1:500 ...
-
bioRxiv - Immunology 2023Quote: ... in FACS-blocking buffer (mixture of 0.66% human/rabbit/mouse serum, Sigma-Aldrich, and 1% Bovine Serum Albumin, Sigma-Aldrich) for 40 minutes at 4 °C ...
-
bioRxiv - Immunology 2023Quote: ... After a further 1 hour incubation at 37°C plates were washed and 50 μl 1:2000 diluted cross-adsorbed anti-mouse HRP (Sigma) was added to each well and incubated for 1 hour at 37°C ...
-
bioRxiv - Developmental Biology 2023Quote: ... blots were incubated overnight at 4°C at the following dilutions: Mouse α-FLAG (1:1000, M2 clone, Sigma #F3165), Mouse α-V5 (1:1000 ...
-
bioRxiv - Microbiology 2023Quote: Protein extract from leaf tissue was incubated for 2h at 4°C with an anti-flag mouse antibody at 1:4,000 dilution (Sigma, F1804), followed by a 1h-incubation at 4°C with PureProteomeProtein A/G Magnetic Beads Mix (Millipore) ...
-
bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 lung titers were quantified by homogenizing mouse lungs in 1ml phosphate-buffered saline (PBS; Quality Biological Inc.) using 1.0mm glass beads (Sigma Aldrich) and a Beadruptor (Omni International Inc) ...
-
bioRxiv - Neuroscience 2023Quote: ... After treatment with the secondary antibody (anti-rabbit HRP-conjugated, 1:1000 or anti-mouse HRP-conjugated, 1:1000, Sigma), membranes were incubated in chemoluminescent (SuperSignal West Femto ...
-
bioRxiv - Cell Biology 2023Quote: The proximal ligation assay (PLA) was performed using the Duolink In Situ Orange Starter Kit Mouse/Rabbit (DUO92103; Sigma-Aldrich), according to the manufacturer’s protocol ...
-
Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... Immunofluorescence in human cells was conducted as previously described using antibodies against tubulin (1:1000 anti-beta tubulin, mouse, T7816, Sigma OR 1:2000/1:1000 anti-alpha tubulin ...
-
bioRxiv - Neuroscience 2023Quote: ... and primary antibodies (SDC1 Rabbit Thermo Fisher Scientific, MA5-32600, 1:500 dilution; GFAP mouse Sigma G3893, 1:500 dilution). After extensive PBS washings ...
-
bioRxiv - Neuroscience 2023Quote: ... then with the primary antibodies in the same solution overnight at 4°C (SDC1, Rabbit Thermofisher MA5-32600, 1:500 dilution; GFAP, mouse Sigma G3893 ...
-
bioRxiv - Neuroscience 2023Quote: ... and incubated for 2 hours with secondary antibody (Goat anti-mouse IgG-HRP or Goat anti-rabbit IgG-HRP, Sigma) diluted in blocking buffer ...
-
bioRxiv - Immunology 2023Quote: ... Mouse monoclonal anti-β-actin (A5441, clone AC-15) and rabbit polyclonal anti-Calnexin (#C4731) were obtained from Sigma-Aldrich. Mouse monoclonal anti-V5 (BLD-680605 ...
-
bioRxiv - Cancer Biology 2023Quote: ... mouse NAFs were transduced using lentiviruses containing shRNA constructs against Postn with 10 mg/ml polybrene (Sigma, TR-1003-G). shPostn1 (F primer ...
-
bioRxiv - Pathology 2023Quote: ... total RNA was extracted from the aortic vessels isolated from each mouse using Trizol Reagent (Sigma Aldrich, St. Louis, MO), per manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... Incubation with primary antibodies was performed overnight at 4°C at the following concentrations: FLAG (mouse, 1:1000; Sigma F1804), HA (mouse ...
-
bioRxiv - Neuroscience 2023Quote: ... for 1 hr at room temperature (RT) and incubated with mouse monoclonal anti-aggrecan neoepitope antibody (10 µg/ml; clone AF-28, MAB19310-C, Millipore) and rabbit polyclonal anti-α-tubulin antibody (2 µg/ml ...
-
bioRxiv - Microbiology 2023Quote: ... or anti-Ace-2 antibody (ab15348) followed by the respective secondary antibodies (horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG; Sigma). Mouse-monoclonal anti-β-actin-peroxidase antibody (A3854 ...
-
bioRxiv - Immunology 2022Quote: ... The plates were then washed and incubated with 100 μl per well of samples prepared as described above and with mouse anti-human αvβ6 (Millipore, MAB2077Z) from 312.5 ng/ml to 1.22 ng/ml to produce the standard curve for 60 min at room temperature ...
-
bioRxiv - Microbiology 2023Quote: ... and probed for EGFP with rabbit anti-EGFP diluted 1:5000 (kind gift from C. Ellermier) or for FLAG with mouse anti-FLAG M2 diluted 1:500 (Milipore-Sigma).
-
bioRxiv - Plant Biology 2023Quote: ... Membranes were washed and further incubated with secondary antibody (goat anti-mouse AP, 1:15000; Sigma Aldrich, St. Louis, MO). The membrane was washed with TBS-Tween 20 (TBS-T ...
-
bioRxiv - Biochemistry 2023Quote: Mouse monoclonal anti-FLAG M2 (#F3165) and anti-CD95 mAb CH-11 (#05-201) was from sigma-Aldrich (Sigma Aldrich Chimie ...
-
bioRxiv - Biophysics 2023Quote: ... Anti-α-tubulin and anti-Nup153 antibodies were respectively revealed by ATTO647N goat anti-mouse IgG (1:400, Sigma-Aldrich) and STAR580 goat anti-rabbit IgG (1:400 ...
-
bioRxiv - Genomics 2023Quote: C57BL/6 mouse ESC were purchased from ATCC (SCRC1002) and maintained onto 1% gelatin-coated (Sigma-Aldrich, Cat. No G1393) dishes in DMEM knockout medium (Life Technologies ...
-
bioRxiv - Microbiology 2023Quote: ... Plates were then washed 3x with 200μLs of PBS-T per well and anti-mouse IgG HRP-conjugated secondary (Sigma, #AP308P) diluted at 1:3000 in blocking solution was added to each well and incubated for 1 hour at room temperature ...
-
bioRxiv - Biochemistry 2023Quote: ... The proteins were resolved in SDS-PAGE gels and transferred to nitrocellulose membranes that were incubated overnight at 4 °C with one of the following antibodies: mouse monoclonal anti-hemagglutinin (1:2000, Sigma); rabbit anti-P-TrkB-Tyr674/675 (1:1000 ...
-
bioRxiv - Biochemistry 2023Quote: ... and mouse BRCA2 (amino acid residues: 2232-2283) were cloned into pMAT11 (Peranen et al., 1996) and pRSF-Duet1 (Merck Millipore) vectors for expression with an N-terminal TEV-cleavable His6-MBP tag and an N-terminal TEV-cleavable MBP tag ...
-
bioRxiv - Cancer Biology 2023Quote: Antibodies used in this study are as follows: mouse monoclonal anti-β-tubulin antibody (Sigma-Aldrich, Cat# T4026, 1:5000), mouse monoclonal anti-FLAG M2 antibody (Sigma-Aldrich ...
-
bioRxiv - Plant Biology 2023Quote: ... as primary antibodies and Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L) (115-035-003, Jackson) or Peroxidase Conjugated Goat Anti-Rabbit IgG (H+L) (AP132P, Sigma) as secondary antibodies ...
-
bioRxiv - Systems Biology 2023Quote: ... All membranes were blocked in a 5% milk solution and then incubated for 12h at 4°C with either the mouse anti-phosphotyrosine 4G10 (05321, Millipore) or the rabbit anti-GFP (Thermo A11122 ...
-
bioRxiv - Cell Biology 2023Quote: ... mouse monoclonal anti-PP2A C-subunit clone 1D6 conjugated with Alexa Fluor 488 (Sigma-Aldrich, 05-421-AF488, 1:50). Anti-human Cy3 (Jackson ImmunoResearch ...
-
bioRxiv - Genomics 2023Quote: ... Expression of the Dam-POI in the mouse ESC lines was suppressed by addition of 0.5 mM indole-3-acetic acid (IAA; Sigma, I5148).
-
bioRxiv - Cell Biology 2023Quote: ... and incubated as described above with a 0.5 μg/ml mouse α-tubulin antibody (clone B-5 1-2, Sigma) as a loading control.
-
bioRxiv - Microbiology 2023Quote: ... were also challenged with mouse peritoneal macrophages (Nrf2 +/+) or (Nrf2 -/-) pre-treated or not with ML385 at 10µM (Sigma-Aldich) or h-MDM pre-treated with ML385 at 10µM (Sigma-Aldich) ...
-
bioRxiv - Synthetic Biology 2023Quote: Stable cell lines expressing human or mouse PRLR were generated by lentiviral transduction followed by 0.7 ug/mL puromycin selection (Sigma; P9620). 12ug of each pTwist Lenti Puro SFFV WPRE lentiviral construct encoding either human or mouse PRLR were co-transfected with 9ug of psPAX2 (Addgene ...
-
bioRxiv - Pathology 2023Quote: Genomic DNA was isolated from mouse tail biopsies using the GenElute Mammalian Genomic DNA extraction kit (Millipore-Sigma, G1N350-1KT) according to the manufacturer’s instructions ...
-
bioRxiv - Pathology 2023Quote: Genomic DNA was isolated from mouse tail biopsies using the GenElute Mammalian Genomic DNA extraction kit (Millipore-Sigma, G1N350-1KT) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... Acyl-ghrelin concentrations in mouse plasma samples were determined by commercial ELISA kits (EMD Millipore, Billerica, MD; Catalog #EZRGRA-90K) (91).
-
bioRxiv - Molecular Biology 2023Quote: ... CRHR2α expression levels in the stably transfected cell lines was assessed by Western blot using a mouse monoclonal anti FLAG -antibody (1:5000 dilution; SIGMA). All the experiments were performed at least 3 times with 3 individual clones (clones 4 ...
-
bioRxiv - Neuroscience 2023Quote: ... brain homogenate from a RML inoculated C57BL6/J mouse at the terminal phase of disease was treated with 100 μg/ml of pronase E (Sigma) for 30 min at 37 °C with shaking in a thermal mixer at 800 rpm ...
-
bioRxiv - Neuroscience 2023Quote: ... diluted in PBS for 1 h before immunoreaction with the following antibodies: mouse anti-GFAP (1:1,000; Millipore, Cat. MAB360), rabbit anti-GFAP (1:1,000 ...
-
bioRxiv - Microbiology 2023Quote: ... incubated for 1 h at room temperature with an anti-mouse IgG secondary antibody conjugated with peroxidase (Sigma Aldrich, A9044) diluted 1:10,000 in blocking buffer ...
-
bioRxiv - Immunology 2023Quote: ... 8 mg of purified anti-FH OX23 antibody (MRC Immunochemistry Unit, Oxford) from mouse hybridoma cell line culture supernatant was immobilised on CNBr-activated Sepharose (Sigma) (2 mg/ml packed Sepharose) ...
-
bioRxiv - Molecular Biology 2023Quote: ... isolated macular retinal nuclei were stained with a mouse anti-NeuN monoclonal antibody (1:5000, Alexa Flour 488 Conjugate MAB377X, Millipore, Billerica ...
-
bioRxiv - Cancer Biology 2023Quote: The formation of DNA damage-induced γ-H2AX foci was analyzed by Immunofluorescence staining using a mouse monoclonal anti-phospho-Histone H2A.X (Ser139) (Sigma) as described before 40 ...
-
bioRxiv - Cancer Biology 2023Quote: ... PLA reactions were subsequently carried out using Duolink PLA plus and minus probes for rabbit and mouse (respectively) and Duolink In Situ Detection Reagents Orange (Sigma) following the manufacturer’s protocol ...