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Citations for Millipore Sigma :
9751 - 9800 of 10000+ citations for Carbonic Anhydrase 14 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... a complementary DNA (cDNA) fragment encoding amino acids 448 to 622 of mouse Zcwpw1 was inserted into the p-ET-32a + vector (EMD Millipore) and transfected into BL21-CodonPlus (DE3 ...
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bioRxiv - Microbiology 2021Quote: ... the following were incubated for 15-18hrs at 4°C in PBS with 0.3% Triton-X and 5% goat serum: mouse biotin conjugated anti-NeuN B-clone A60 (MAB377, Millipore, 1:500), rabbit anti-calbindin (C2724 ...
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bioRxiv - Microbiology 2019Quote: ... Membranes were then washed and probed with either goat anti-mouse horseradish peroxidase or goat anti-rabbit horseradish peroxidase (Sigma-Aldrich) at a dilution of 1:10000 for 1 hour (GE Healthcare) ...
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bioRxiv - Microbiology 2019Quote: ... The conditioned media was filter-sterilized and cytokines and chemokines were measured using Milliplex MAP Mouse Cytokine/Chemokine Magnetic Bead Panel (MCYTOMAG-70K, Millipore Sigma) and Procartaplex Mouse IFNα/IFNβ Panel (2plex ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by immunocytochemistry with mouse monoclonal anti-vinculin antibody (1:200 dilution in 1% BSA–PBS) (Sigma; V9264, clone hVIN-1) and Alexa 555–labelled goat anti–mouse IgG (#A21422 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were incubated at 37ºC for 1 h with Duolink® in situ PLA® Anti-rabbit PLUS and Anti-mouse MINUS (Sigma) and later washed with 0,01% PBS-tween ...
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bioRxiv - Developmental Biology 2020Quote: ... Zygotes were collected from the oviduct of superovulated females 20h post HCG injection and cultured in EmbryoMax® KSOM Mouse Embryo Media (Millipore) at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then incubated for 1hr with primary antibodies diluted as follows with Block buffer: mouse monoclonal anti-FLAG (Sigma Aldrich) at 1:500 ...
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bioRxiv - Molecular Biology 2022Quote: Tipless silicon nitride AFM cantilevers (NanoWorld, PNP-TR-TL) were coated with monoclonal anti-HA antibodies produced in mouse (H3663, Sigma-Aldrich) or Fc-control for the antibody-based mechano-activation experiments using flexible PEG spacers as described before (Ebner et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by incubation with 3-isobutylmethyl-xanthine (1 mM)-containing medium or including the secondary antibody anti-mouse IgG (Fc specific, M2650, Sigma-Aldrich) for 30 min ...
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bioRxiv - Developmental Biology 2022Quote: Wild-type E14 mouse ES cells (Sarah Kinkley Lab) were cultured without feeders on gelatin-coated plates (0.1% gelatin, Sigma-Aldrich G1393). The media was changed every day and cells were passaged every 2 days ...
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bioRxiv - Developmental Biology 2022Quote: We developed an injury-mediated mouse model of skeletal muscle regeneration by intramuscular injection of cardiotoxin (CTX) from Naja nigricollis (EMD Millipore) following our established procedure as described earlier [27 ...
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bioRxiv - Neuroscience 2021Quote: ... This was directly followed by incubation overnight at room temperature with primary antibodies (GFAP; host: mouse; diluted 1:1000; Sigma-Aldrich; Cat# MAB360 ...
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bioRxiv - Neuroscience 2021Quote: Mice were sacrificed and the hippocampus of each mouse was dissected and placed in chilled Hanks’ Balanced Salt Solution (Sigma #55021C). The “Frankenstein” protocol [ref] was followed to obtain a nuclei suspension ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibody reaction was carried out for 10 days at 4°C in blocking solution (1:1000 mouse monoclonal Tyrosine Hydroxylase antibody; T2928, Sigma-Aldrich). Sections were then incubated in biotinylated goat-anti-mouse antibody for 24 hours at 4 °C in blocking solution (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... chicken anti-GFAP (Chemicon, AB5541, 1:3000, RRID:AB_177521), mouse anti-SMI-32 (Covance, SMI-32R, 1:2000) and Hoechst 33342 (Sigma Aldrich, 1:300) for nuclear labeling ...
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bioRxiv - Molecular Biology 2021Quote: ... Larvae were blocked in 10% sheep serum in PBDT for 1 hour at room temperature and then incubated in mouse anti-alpha acetylated tubulin (Sigma-Aldrich Canada Ltd ...
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bioRxiv - Neuroscience 2021Quote: ... washed as above described and re-blotted using mouse anti-β-actin antibody (Sigma-Aldrich, USA; 1:2500, 1 h, RT). Again ...
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bioRxiv - Molecular Biology 2020Quote: ... and incubated in blocking solution [PBS with 3% bovine serum albumin prior to the addition of a primary mouse monoclonal antibody specific for phospho-Histone H2AX (Ser13; clone JBW301; Millipore Sigma). Following incubation ...
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bioRxiv - Cell Biology 2022Quote: ... followed by probing with horse radish peroxidase (HRP)-conjugated α-mouse (1:10000) (Santa-Cruz Biotech) and detection of signal after chemiluminescent HRP substrate (Millipore) treatment.
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bioRxiv - Cell Biology 2022Quote: ... The cDNA encoding mouse BANF1/BAF was synthesized using two120-mers and a 90-mer single-stranded DNA oligonucleotides (Table 2, Sigma-Aldrich). These ssDNA oligonucleotides each contain a 15-mer overlapping sequence at both ends ...
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bioRxiv - Microbiology 2019Quote: Mouse hnRNPC (EMU173751)-targeting Endonuclease prepared short interfering RNA (esiRNA) and scrambled esiRNA as negative-control were purchased from Sigma-Aldrich. Neuro2A cells upon achieving 60% confluency were undergone transfection with 20 Pico mole of hnRNPC esiRNA along with lipofectamine RNAiMax (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... anti-GFP (in house made rabbit polyclonal antibody; 1:1,000-4,000) anti-Vinculin (mouse monoclonal; clone hVIN-1; Sigma-Aldrich; 1:10000), anti PMF1/NNF1 (in house made mouse affinity purified monoclonal ...
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bioRxiv - Immunology 2019Quote: ... cells were washed twice with PBS for 1 h at 37°C and then incubated with a FITC-conjugated rabbit anti-mouse IgG antibody (Sigma-Aldrich) at a 1:500 dilution ...
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bioRxiv - Microbiology 2019Quote: ... in 0.1 M phosphate buffer to block non-specific binding then incubated with the primary antibodies: mouse anti-RSV fusion protein monoclonal antibody (MAB8599, Millipore, USA) or goat anti-human IgG (Licor ...
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bioRxiv - Cancer Biology 2019Quote: ... the TMA slide was subjected to Cell Conditioning 1 (CC1, Ventana) antigen retrieval and stained with a prediluted anti-MCT antibody (Mouse, Clone G3, Millipore #MAB1222) using an automated immunostainer ...
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bioRxiv - Plant Biology 2019Quote: ... 10 µL protein G Dynabeads® were washed twice in ChIP dilution buffer and incubated either with 2 µg of mouse IgG (Sigma), anti-H3K27me3 (Abcam ab6002 ...
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bioRxiv - Developmental Biology 2019Quote: ... incubated 12 min with 5% Triton in PBS and then primary cilia and SMO were stained using a mouse anti-acetylated tubulin Ab (6-11B-1, Sigma-Aldrich) and a rabbit anti-SMO Ab (1:100 ...
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bioRxiv - Cell Biology 2019Quote: ... K124 and K42 immunoblots were reprobed with anti-keratin K14 and mouse anti-β-actin-HRP mAb (K124 blots; 1:15,000, clone AC-15; Sigma-Aldrich) or anti-β-actin-HRP alone (K42 blots ...
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bioRxiv - Molecular Biology 2019Quote: ... NF-kB (CST, #3033), PCM1 (CST, #5213) and GABARAP (CST, #26632) and NDP52 (. Secondary antibodies: anti-Mouse IgG–Peroxidase (Sigma, A5906), anti-Rabbit IgG–Peroxidase (Sigma ...
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bioRxiv - Developmental Biology 2019Quote: ... cell lysates were subjected to immunoprecipitation overnight using an anti-mouse HSF2 (Santa-Cruz) and a non relevant IgG (Sigma-aldrich) as a negative control that were pre-incubated 1h at RT with Protein G UltraLink Resin beads (53132 ...
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bioRxiv - Neuroscience 2019Quote: ... Five 15 cm dishes of 70 – 80 % confluent mouse Schwann cells were separately transfected with 30 μg of pLKO.1-puro vector containing five commercial VDAC1 shRNA (Sigma-Aldrich, sh1#TRCN0000012388 ...
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bioRxiv - Microbiology 2019Quote: ... The plate was washed three times with wash buffer (PBS/0.1% Tween-20) and the primary antibody (anti-NP mouse monoclonal Ab, Millipore, cat. MAB2851) added in blocking buffer ...
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bioRxiv - Biochemistry 2019Quote: ... the membranes were incubated with HRP-coupled secondary antibodies diluted 1:10,000 in BS (anti-mouse IgG Jackson 515-035-062, anti-rabbit IgG Sigma A-9169), washed twice in BS and twice in TBS ...
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bioRxiv - Biophysics 2019Quote: ... the cells were incubated for 1 hour at 37°C with 1:300 mouse anti-α-tubulin antibody (Sigma Aldrich, T6199) in PBS + 1% BSA ...
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bioRxiv - Biochemistry 2019Quote: ... rabbit monoclonal DYKDDDDK tag (Flag tag, 1:1000, CST, #14793) and/or mouse monoclonal α-tubulin (1:10,000, Millipore, 05-829). Following three washes with TBS-T ...
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bioRxiv - Microbiology 2020Quote: ... Membrane was further blocked with 5% milk in 1X PBS and Tween 20 for 30 min followed by incubation with mouse anti-gE monoclonal antibody (clone 8612, EMD Millipore) (1:3,000 ...
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bioRxiv - Genetics 2019Quote: ... paraffin-embedded and stained using anti-RAS (G12S) mouse monoclonal antibody (NewEast, 26186) followed by incubation with HRP-conjugated corresponding secondary antibody (Sigma-Aldrich). The expression levels were evaluated by H-score method ...
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bioRxiv - Developmental Biology 2019Quote: Single cell suspensions of wild type AGM and YS at E9.5 and E11.5 were stained with rat anti-mouse CD144-efluor660 1:200 (eBioscience #50-1441-82, clone eBIOBV13) and 7AAD (Sigma, #A9400). Cells were analyzed and sorted on FACSAria (BD) ...
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bioRxiv - Immunology 2021Quote: ... and IP-10) were measured relative to unstimulated cells in supernatants using a Milliplex MAP Magnetic Mouse Cytokine/Chemokine multiplex immunoassay (EMD Millipore).
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bioRxiv - Immunology 2021Quote: ... bound antibodies were detected by addition of a 1 in 5000 dilution of alkaline phosphatase (AP)-conjugated goat anti-mouse IgG (Sigma-Aldrich) for 1 hour at 21°C and addition of p-Nitrophenyl Phosphate ...
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bioRxiv - Microbiology 2021Quote: ... DivK protein separated based on its phosphorylation state was transferred to a PVDF membrane and detected using a chemiluminescent substrate (SuperSignal West PICO PLUS, Thermo Pierce) after incubation with mouse anti-Flag (1:10000) primary antibody (Sigma, F1804) and a polyclonal goat anti-mouse HRP (1:10000 ...
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bioRxiv - Synthetic Biology 2021Quote: ... incubated with secondary anti-Rabbit IgG-Gold coupled antibody (1/10 in BHN1) (Anti-Mouse IgG (whole molecule)–Gold antibody produced in goat (Sigma, G7652), washed twice in BHN2 and HN (50 mM HEPES ...
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bioRxiv - Physiology 2021Quote: ... in maternal serum and placenta was performed with MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel – Immunology Multiplex Assays (MCYTOMAG-70K, Merck Millipore, Germany) following the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 hour before incubation with primary antibodies: rabbit anti-NogoA (1:200; Supplementary Table 4) and mouse anti-GFAP (1:500; Sigma-Aldrich), for 72 hours at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... They were blocked in 1% BSA in PBT for 1hr at RT and incubated overnight at 4°C with the monoclonal mouse anti-FLAG M2 antibody (Sigma, F1804) diluted 1:500 in 1% BSA/PBS ...
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bioRxiv - Cell Biology 2021Quote: Immunoprecipitations of 3XF-PPIA and 3XF-Mutant PPIA transiently transfected cells were performed with a mouse monoclonal anti-FLAG antibody (clone M2, Millipore Sigma) in 293T cells ...
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bioRxiv - Cell Biology 2021Quote: ... proximity ligation assays were performed on isolated HSCs with the Duolink in Situ Red Starter Kit Mouse/Rabbit (DUO92101, Millipore Sigma), adapting the vendor’s protocol for HSCs ...
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bioRxiv - Neuroscience 2020Quote: ... 16 μm) were incubated for 2 h at 30°C with Mouse anti-TH antibody (1:1000, MAB318, Millipore, Burlington, MA) in DEPC-treated phosphate buffer (PB ...
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bioRxiv - Neuroscience 2020Quote: ... as well as secondary anti-mouse and anti-rabbit (Thermo Fischer Scientific) Membranes were incubated with Immobilon® ECL Ultra Western HRP Substrate (Millipore) for 3 minutes prior to image acquisition ...