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Citations for Millipore Sigma :
9601 - 9650 of 10000+ citations for Carbonic Anhydrase 14 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated overnight at 4 °C with antibodies that recognise specific proteins expressed by each cell type such as mouse anti-NeuN (1:250; Millipore) to label neurons ...
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bioRxiv - Cell Biology 2024Quote: ... TRPM2-FLAG protein was probed with mouse anti-FLAG®M2 antibodies (F1804, Sigma; primary, 1:5000 in blocking buffer) in conjunction with HRP-conjugated goat anti-mouse IgG (A16078 ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed three times with PBS and incubated 1hr/37°C/5%CO2/rocking with horseradish peroxidase (HRP)-conjugated goat anti-mouse antibody (Sigma), diluted 1:1,500 in blocking buffer ...
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bioRxiv - Evolutionary Biology 2024Quote: Mouse and macaque endometrial epithelial organoids were treated for two consecutive days with 17-ß estradiol (E2) (Sigma Aldrich, E8875) at 10 nM or for two days with 17-ß estradiol (E2 ...
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bioRxiv - Microbiology 2023Quote: ... were also challenged with mouse peritoneal macrophages (Nrf2 +/+) or (Nrf2 -/-) pre-treated or not with ML385 at 10µM (Sigma-Aldich) or h-MDM pre-treated with ML385 at 10µM (Sigma-Aldich) ...
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bioRxiv - Synthetic Biology 2023Quote: Stable cell lines expressing human or mouse PRLR were generated by lentiviral transduction followed by 0.7 ug/mL puromycin selection (Sigma; P9620). 12ug of each pTwist Lenti Puro SFFV WPRE lentiviral construct encoding either human or mouse PRLR were co-transfected with 9ug of psPAX2 (Addgene ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: Mouse cortices from embryonic day 18 (E18) embryos were dissected into ice-cold HBSS and dissociated in acetylated trypsin (Sigma) for 20 min at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... and rat anti-MBP (1:500; Serotec) antibodies (OPCs) or 2 h incubation with mouse anti-GFAP (1:200, Sigma) (astrocytes) ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated for 1h at room temperature in blocking solution (5% non-fat dried milk and 0.1% Tween 20 in PBS) and then overnight at 4°C with the primary antibody (mouse anti-β-ACTIN, 1:30,000, Sigma; rabbit anti-AKT1 ...
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bioRxiv - Genomics 2022Quote: ... milk powder in TBS-T (Tris-buffered saline-Tween-20) and incubated overnight with primary antibody in 3% BSA/TBS-T (monoclonal mouse anti-FLAG, 1:1,000; Sigma-Aldrich, #F1804-1MG ...
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bioRxiv - Biochemistry 2022Quote: ... Then each membrane was incubated with the corresponding primary antibody (mouse IgG anti-Lewis antigen, Sigma–Aldrich, 1:500 dilution) for 1h at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... the membranes were washed with PBS three times and then incubated with secondary antibodies: horseradish peroxidase-conjugated anti-mouse IgG and anti-rabbit IgG antibodies (Millipore, Billerica ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were fixed 72 h after transfection in -20°C methanol and subjected to PLA using the Duolink In Situ Red Starter Kit Mouse/Rabbit (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Percentage of infected cells were measured by intracellular staining for the nucleoprotein (mouse anti-coronavirus OC43 nucleoprotein clone 542-70, Millipore).
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Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: ... The primary antibodies used in immunofluorescence (IF) were rabbit anti-LvIRF antibody (Genecreate) and mouse anti-β-actin antibody (Sigma). The slides were washed with PBS six times and then incubated with diluted second antibodies of anti-rabbit IgG (H+L ...
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bioRxiv - Immunology 2022Quote: ... growth factors) in mouse whole lung protein were quantified using EMD Millipore’s MILLIPLEX Magnetic Bead Panel (Millipore Sigma #MCYTOMAG-70K), including ...
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bioRxiv - Microbiology 2023Quote: ... Expression of WT and mutant N was assessed by Western blot using a rabbit anti-N antiserum 39 and a mouse monoclonal anti-tubulin antibody (Sigma), revealed by incubation with anti-rabbit and anti-mouse antibodies coupled to HRP (SeraCare).
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bioRxiv - Microbiology 2023Quote: ... protein (1:50 dilution) (BEI Resources, NR-619) and the secondary anti-mouse IgG antibody conjugated with FITC (1:100 dilution, Sigma). The cell nuclei were stained with DAPI ...
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bioRxiv - Neuroscience 2023Quote: ... and tumor necrosis factor α (TNFα)) was performed using the Milliplex Mouse Cytokine Magnetic Kit (EMD Millipore, Cat # MCYTOMAG-70K) and Luminex MAGPIX instrument at the Vanderbilt Hormone Assay and Analytical Service Core ...
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bioRxiv - Plant Biology 2023Quote: ... The sections were blocked with 5% (w/v) bovine serum albumin (BSA) in PBS and incubated then with an anti-HA mouse monoclonal antibody (Sigma) for 2 h at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... γ-H2AX foci were detected with mouse monoclonal antibody anti-phospho-Histone H2AX (Ser139) (1:1000 dilution; Cat #05-636, Millipore) and goat anti-mouse IgG secondary antibody ...
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bioRxiv - Cell Biology 2023Quote: ... gift from Christian Ungermann) as well as respective peroxidase-conjugated secondary antibodies against mouse (dilution 1:10,000, rabbit, Sigma A9044) or rabbit (dilution 1:10000 ...
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bioRxiv - Neuroscience 2023Quote: ... which were blocked in 5% dry milk powder in TBS-T for 1 hour and then incubated in primary antibody solutions (Rabbit anti NUDT6, Proteintech 1:800; mouse anti FGF2, Millipore, 1:500 ...
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bioRxiv - Genetics 2023Quote: ... membranes were incubated overnight at 4°C with primary antibodies in a 1:1 solution of TBST (TBS + 0.05% Tween20) and LiCor blocking buffer (Rabbit anti-GFP, Torrey Pines Scientific TP401; and Mouse anti-alpha-tubulin, Sigma T5168). Membranes were then washed 5x times with TBST ...
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bioRxiv - Biophysics 2023Quote: ... Anti-α-tubulin and anti-Nup153 antibodies were respectively revealed by ATTO647N goat anti-mouse IgG (1:400, Sigma-Aldrich) and STAR580 goat anti-rabbit IgG (1:400 ...
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bioRxiv - Biochemistry 2023Quote: ... and mouse BRCA2 (amino acid residues: 2232-2283) were cloned into pMAT11 (Peranen et al., 1996) and pRSF-Duet1 (Merck Millipore) vectors for expression with an N-terminal TEV-cleavable His6-MBP tag and an N-terminal TEV-cleavable MBP tag ...
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bioRxiv - Genomics 2023Quote: C57BL/6 mouse ESC were purchased from ATCC (SCRC1002) and maintained onto 1% gelatin-coated (Sigma-Aldrich, Cat. No G1393) dishes in DMEM knockout medium (Life Technologies ...
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bioRxiv - Microbiology 2023Quote: ... France) and secondary (anti-mouse IgG peroxidase-conjugated, 1:1000 in PBS + 1% BSA, Sigma Aldrich, St. Louis, MO, USA) antibodies were added at RT for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 20 ng/mL mouse GM-CSF (Miltenyi #130-095-739) and 0.5 μM β-estradiol (Sigma-Aldrich #E2758)] and transduced with 1:5 (vol:vol ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies coupled to horseradish peroxidase (HRP) were anti-rat-HRP at 1:10000 and anti-mouse-HRP at 1:10000 (Sigma).
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bioRxiv - Cancer Biology 2023Quote: Proximity ligation assays (PLA) were performed using the Duolink® In Situ Red Kit Mouse/Rabbit (Sigma-Aldrich, Cat # DUO92101). For in vitro PLA ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatant solution was removed and the cells were treated with a secondary antibody (the FITC-conjugated anti-mouse IgG (Sigma)) for one hour at 37°C in the darkness ...
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bioRxiv - Neuroscience 2023Quote: ... 0.1% Tween in TBS at room temperature and incubated overnight at 4 °C with primary antibodies against digoxigenin (mouse monoclonal 1:500, Sigma) and β-actin (rabbit polyclonal 1:200 ...
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bioRxiv - Neuroscience 2023Quote: Western blotting of adult head lysates (three heads per sample with one head loaded per lane) was performed using standard laboratory procedures with mouse anti-Tubulin (T5168; Sigma) at 1:10000 (UAS rescue experiments ...
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bioRxiv - Molecular Biology 2023Quote: ... Full-length FN and 45 kDa-FN GBD were detected with monoclonal mouse anti-FN antibody with epitope within GBD (MAB1892, Sigma), clone IST-10 (1:1000 in 2% BSA in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... S4 a similar protocol was used but the primary antibody mix consisted of mouse anti-GFP 1:1000 (Sigma G6539), rabbit anti-RFP 1:5000 (Rockland 600-401-379) ...
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bioRxiv - Cell Biology 2023Quote: ... The following antibod-ies were used for western blots of human cell extracts: Mouse anti-FLAG (Sigma F1804, 1:1000); Mouse anti-GAPDH (Proteintech 60004-1-Ig ...
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bioRxiv - Physiology 2023Quote: Free fatty acid (FFA) content was quantified in mouse plasma samples using the Free Fatty Acid Quantitation Kit (Sigma, #MAK0044) in line with the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... PLA reactions were subsequently performed using Duolink PLA probes for goat-mouse and goat-rabbit and Duolink In Situ Detection Reagents Red (Sigma) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... Samples were then transferred to a PVDF membrane and analyzed by Western blot using a mouse-anti-myc primary antibody (Sigma) and HRP-coupled goat-anti-mouse secondary antibody (Jackson ImmunoResearch) ...
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bioRxiv - Cell Biology 2023Quote: The following antibodies were used in this study: monoclonal mouse antibody to BIN1 (clone C99D against exon 17) from Millipore, polyclonal rabbit antibody to IRSp53 (BAIAP2 ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated with secondary-HRP conjugated antibodies (1:10.000; Anti Rabbit IgG HRPO, Millipore BI2407; Anti Mouse IgG HRPO, Millipore BI2413C) for 1h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with the primary antibody OC (1:500 for mouse, 1:200 for human; polyclonal, AB2286, Millipore) and respective secondary antibody (1:400 ...
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bioRxiv - Cell Biology 2023Quote: ... Rabbit polyclonal anti-pS226 GLUT1 antibody (ABN991) and mouse anti-phosphotyrosine monoclonal antibody cocktail 4G10 Platinum (05–1050) were purchased from Millipore. Recombinant polyclonal anti-ALDOA antibody cocktail (711764 ...
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bioRxiv - Microbiology 2023Quote: ... The secondary antibodies used for IB were goat anti-rabbit (Cat # AP307P) and goat anti-mouse (Cat # AP308P) IgG HRP-conjugated antibodies (Merck Millipore).
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bioRxiv - Biochemistry 2023Quote: ... The proteins were resolved in SDS-PAGE gels and transferred to nitrocellulose membranes that were incubated overnight at 4 °C with one of the following antibodies: mouse monoclonal anti-hemagglutinin (1:2000, Sigma); rabbit anti-P-TrkB-Tyr674/675 (1:1000 ...
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bioRxiv - Biochemistry 2023Quote: Mouse monoclonal anti-FLAG M2 (#F3165) and anti-CD95 mAb CH-11 (#05-201) was from sigma-Aldrich (Sigma Aldrich Chimie ...
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bioRxiv - Biochemistry 2023Quote: ... the plates were incubated with 100 μL/well horseradish peroxidase-conjugated anti-mouse immunoglobulin second antibody (Sigma, St. Louis, MO) in Buffer D of pCa 9 or 4 at room temperature for 1 h ...
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bioRxiv - Cancer Biology 2022Quote: ... were cultured in the presence of recombinant mouse IL-4 (25 ng/mL; #404-ML, R&D) and LPS (20 μg/mL; #L2880, Sigma) over night to induce B cell activation and terminal differentiation ...
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bioRxiv - Cell Biology 2023Quote: ... mESCs were grown at 37°C and 5% CO2 on Mitomycin-C inactivated feeder cells (DR4-mouse embryonic fibroblast) plated on 0.1% gelatin (Sigma-Aldrich) in serum/leukemia inhibitory factor (LIF ...