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Citations for Millipore Sigma :
9701 - 9750 of 10000+ citations for Carbonic Anhydrase 14 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... The abundance of cytokines was determined using a MILLIPLEX Mouse 32-Plex Cytokine Panel 1 kit (Millipore # MCYTMAG-70K-PX32) according to the manufacturer’s instructions.
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: Mouse cortices from embryonic day 18 (E18) embryos were dissected into ice-cold HBSS and dissociated in acetylated trypsin (Sigma) for 20 min at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... sub-confluent mouse embryonic 3T3-J2 fibroblasts were treated for 2 hours with 4µg/ml Mitomycin C (Sigma-Aldrich, M4287), washed three time with PBS ...
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bioRxiv - Biochemistry 2023Quote: ... The protein bands were obtained using horseradish peroxidase-conjugated antibodies against mouse whole immunoglobulins and horseradish peroxidase-conjugated antibodies against rabbit whole immunoglobulins (1∶10,000, Sigma-Aldrich) as secondary antibodies and detection with the Lumi-Light Plus Western Blotting Substrate (Roche) ...
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bioRxiv - Cancer Biology 2023Quote: ... Resulting immunocomplexes were detected by HRP-conjugated anti-mouse IgG or anti-rabbit IgG secondary antibodies and enhanced chemiluminescent (ECL) reagents (WBLUF0100, Merck Millipore).
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CRISPR-Cas9 Engineered Extracellular Vesicles for the Treatment of Dominant Progressive Hearing LossbioRxiv - Bioengineering 2023Quote: Genomic DNA was extracted from mouse pinna by incubating ear punches with 40μL tissue preparation solution (Sigma, Cat No. T3073) and 40μL extraction solution (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 20 ng/mL mouse GM-CSF (Miltenyi #130-095-739) and 0.5 μM β-estradiol (Sigma-Aldrich #E2758)] and transduced with 1:5 (vol:vol ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies coupled to horseradish peroxidase (HRP) were anti-rat-HRP at 1:10000 and anti-mouse-HRP at 1:10000 (Sigma).
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bioRxiv - Cell Biology 2023Quote: ... The following antibod-ies were used for western blots of human cell extracts: Mouse anti-FLAG (Sigma F1804, 1:1000); Mouse anti-GAPDH (Proteintech 60004-1-Ig ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was further incubated 1 hour at 25°C with a second antibody (anti-mouse HRP conjugated antibody; Sigma) diluted 1:10,000 in PBS-Tween ...
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bioRxiv - Immunology 2024Quote: ... mice were bled for serum cytokine analysis using MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel (Millipore MCYTOMAG-70K-PMX) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Slides were then washed twice with PBS and processed using Duolink Proximity Ligation Assay (PLA) reagents (In-Situ PLA Probe Anti-Mouse MINUS, Sigma DUO92004 ...
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bioRxiv - Cell Biology 2024Quote: ... Slides were then washed twice with PBS and processed using Duolink Proximity Ligation Assay (PLA) reagents (In-Situ PLA Probe Anti-Mouse MINUS, Sigma DUO92004; In-Situ PLA Probe Anti-Rabbit PLUS, Sigma DUO92002 ...
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bioRxiv - Molecular Biology 2024Quote: ... Secondary antibodies coupled to horse radish peroxidase (goat anti-rabbit, goat anti-rat, and goat anti-mouse) were purchased from Sigma and used at the recommended dilution.
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bioRxiv - Cancer Biology 2024Quote: ... The tubes were centrifuged at 6,000 rpm and the supernatants were collected for cytokine analysis by bead-based multiplex assay (MILLIPLEX MAP Mouse High Sensitivity T Cell Panel, Millipore) following the manufacturer protocol27.
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bioRxiv - Microbiology 2024Quote: ... α-tubulin was detected as a loading control with the mouse monoclonal antibody B-5-1-2 (Sigma, T5168, 1/4000) for 2 hrs at 25°C ...
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bioRxiv - Neuroscience 2024Quote: ... in primary neuronal lysate after Aβ or gAβ treatment from mouse or human brain were quantified by the Glycerol-3-Phosphate Assay Kit (Sigma), High Sensitivity DHAP Fluorometric Assay Kit (Sigma) ...
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bioRxiv - Neuroscience 2024Quote: ... Then these free-floating tissue sections were incubated with a primary mouse anti-TPH antibody (diluted 1:500, purchased Sigma) for 48 hours at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: Binding of cGAS and cytoplasmic mtDNA in human and mouse brain sections was determined by a PLA kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: Mouse cell lines: HC11 cells (CRL-3062, ATCC) were cultured in RPMI-1640 and supplemented with 10% FBS (F2442, Sigma) and 100 U/ml penicillin ...
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bioRxiv - Bioengineering 2024Quote: ... The primary antibodies used included mouse anti-Integrin alpha 5/CD49e (Snaka51, Catalog Number: MABT201, Sigma-Aldrich, Dilution: 1:500), mouse anti-Integrin alpha V (L230 ...
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bioRxiv - Cell Biology 2024Quote: ... Fixed cells were labeled with a rabbit anti-KIF5B antibody (Tanaka et al., 1998) and a mouse anti-insulin antibody (Sigma), followed by Alexa-conjugated secondary antibodies (Invitrogen) ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were then diluted to 1 mg/ml and processed by Eve Technologies using the Mouse High Sensitive 18-Plex Discovery Assay according to the manufacturer’s protocol (Millipore Sigma).
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bioRxiv - Microbiology 2024Quote: ... Organisms were stained with anti-MOMP (Meridian Biosciences) primary antibody for all the strains and primary mouse anti-Cpf1 (dCas12) (Sigma-Millipore) for knockdown or complementation samples ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1% RNAse-free Bovine Serum Albumin for 30 minutes at RT before being incubated with primary antibodies (Thermofisher, mouse anti-tubulin, 1:1000; Sigma mouse monoclonal anti-myc clone 9E10 ...
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bioRxiv - Immunology 2024Quote: ... and PRL were analyzed in a single run on a Luminex 200 platform using a MILLIPLEX MAP Mouse Pituitary Magnetic Bead Panel (Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: Mouse organs and ES cells were homogenized mechanically in RIPA lysis buffer supplemented with 2x protease inhibitor cocktail set (Millipore) and loaded with SDS dye ...
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bioRxiv - Molecular Biology 2024Quote: For aortic vascular permeability measurements, Evans blue dye (20 mg/ml in saline, 2.5 µl/g mouse body weight) (Sigma E2129) was injected retro-orbitally to LPHN2 endothelial cell-specific knockout and their control littermates at 8 weeks old ...
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bioRxiv - Microbiology 2024Quote: ... 100 µL of overnight cultures grown in M63CA+G was centrifuged and resuspended in 100 µL of PBST (PBS + 0.05% v/v Tween20) containing a 1:100 dilution of mouse monoclonal α-FLAG antibodies (Sigma) and incubated overnight at 4 °C with end-over-end rotation in a microcentrifuge tube ...
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bioRxiv - Microbiology 2024Quote: ... Blots were washed and then probed with a 1:10,000 dilution of goat α-mouse antibody conjugated to horse radish peroxidase (Sigma). Blots were then incubated with SuperSignal West Pico PLUS Chemiluminescence substrate (ThermoFisher ...
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bioRxiv - Immunology 2024Quote: ... The cells were washed three times in PBS 1× and incubated for 1 hour at 37°C with 40 μL of mouse anti-DENV complex monoclonal antibody MAB8705 (Merck Millipore) diluted 1:200 in PBS 1× ...
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bioRxiv - Physiology 2024Quote: Immunoblot analysis was carried out on cell or adipose lysates using mouse or human primary antibodies against MPC1 (Sigma #HPA045119), MPC2 (Cell Signaling Technologies #46141) ...
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bioRxiv - Plant Biology 2024Quote: Immunoblot analyses were performed using horseradish peroxidase (HRP)-conjugated anti-FLAG M2 mouse monoclonal (Sigma-Aldrich A8592, 1:10,000 dilution) and mouse HRP-anti-MYC monoclonal antibodies (BioLegend #626803 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Mouse tissues were diced into pieces and digested in 10 mL RPMI 1640 containing 1mg/mL collagenase-1A (Sigma-Aldrich) make tissues into single-cell suspensions or thorough homogenates by using semi-automated gentleMACS™ Dissociator and incubate for 45 min at 37 °C on an orbital shaker ...
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bioRxiv - Microbiology 2024Quote: ... the membrane was washed three times with TBS-T before incubating with mouse monoclonal anti-FLAG coupled to HRP (#A8592, Sigma) at a 1:5,000 titer in TBS-T ...
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bioRxiv - Cancer Biology 2024Quote: ... and a mouse monoclonal anti-ß-actin antibody (1:5,000, A-5441, Sigma-Aldrich and 1:1,000, A1978, Sigma-Aldrich). Afterwards ...
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bioRxiv - Cancer Biology 2024Quote: ... and a mouse monoclonal anti-ß-actin antibody (1:5,000, A-5441, Sigma-Aldrich and 1:1,000, A1978, Sigma-Aldrich). Afterwards ...
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bioRxiv - Microbiology 2024Quote: ... The HA-immunoprecipitation was performed by incubating the lysated with 50 μL of a 50/50 slurry of mouse monoclonal anti-HA coupled to agarose beads (Millipore-Sigma) for 3 h at 4 °C on a rotating wheel ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Sections through the striatum were stained with a mouse monoclonal anti-TH IgG (1:1,000 dilution; Millipore, Cat# MAB318, RRID:AB_2201528), and then with a goat anti-mouse IgG conjugated with Alexa Fluor 488 (1:400 dilution ...
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bioRxiv - Biophysics 2021Quote: ... glass coverslips (Marienfeld, 0117640) were sonicated in 70% ethanol for 5 min and air-dried before coating with mouse laminin (SIGMA L2020) for 1 h at RT and at a final laminin concentration of 25 μg/mL in coating buffer (50 mM Tris-HCl pH 8 ...
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bioRxiv - Cancer Biology 2021Quote: ... and nascent puromycylated proteins was detected by Western blot analysis as above using a mouse anti-puromycin antibody 12D10 (1:3000)(MABE343, Sigma-Aldrich).
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bioRxiv - Cancer Biology 2021Quote: ... To deplete mouse STK3 expression in HMVP2 cells TRC2 MISSION shRNAs in pLKO.1-puro vector backbone were used (Millipore-Sigma). Lentivirus was generated with psPAX2 and pMD2.G packaging plasmids using with the Polyplus jetPRIME transfection kit in 293T cells ...
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bioRxiv - Cell Biology 2019Quote: ... and immunoblotted with the indicated antibodies followed by HRP-conjugated goat anti–mouse IgG or anti–rabbit IgG or rabbit anti–chicken IgY (Sigma-Aldrich) and Super Signal West Pico Chemiluminescent Substrate (PI34080 ...
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bioRxiv - Cell Biology 2020Quote: ... PLA was performed using Duolink® PLA Fluorescent Detection (red) with anti-mouse PLUS and anti-rabbit MINUS oligonucleotides (Sigma-Aldrich). PLA was performed as described elsewhere (Lin et al. ...
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bioRxiv - Cell Biology 2020Quote: Cells were stained with rabbit anti-GlActin antibody 28PB+1 (Paredez et al., 2011) and mouse monoclonal anti-HA (Clone HA7, Sigma-Aldrich) both diluted 1:125 in PMEBALG and incubated over-night ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with Alexa-488 goat anti-mouse and Alexa-555 goat anti-α-rabbit (Sigma-Aldrich, St. Louis, MO) (diluted 1:150 in PMEBALG ...
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bioRxiv - Cell Biology 2019Quote: ... cells were incubated with Alexa 488/647-conjugated goat anti-mouse or anti-rabbit (1:100-1:400, Thermo Fischer) and Alexa 488/546-conjugated phalloidin (1:200, Sigma-Aldrich) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... The membrane was washed four times (each for 10 min) in TBS-T before being incubated with an anti-mouse horse radish peroxidase (HRP)-conjugated secondary antibody (A9044, Sigma-Aldrich) or an anti-rabbit HRP-conjugated secondary antibody (31466 ...
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bioRxiv - Cell Biology 2020Quote: Lamin A proteins were detected by probing with mouse anti-Myc antibodies (clone 4A6, Millipore cat #05-724; 1:10,000 dilution) decorated with goat anti-mouse secondary IRDye 680RD antibodies (LI-COR ...
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bioRxiv - Developmental Biology 2021Quote: Innervation analysis was done on regenerating limb and flank sections to quantify innervation abundance using the Mouse Monoclonal Anti-Acetylated Tubulin antibody (1:200 dilution – Sigma Aldrich), followed by the Goat-Anti-Mouse IgG Alexa Fluor 488 (1:200 dilution – Abcam ...