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Citations for Millipore Sigma :
701 - 750 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Apoptotic cells were identified by TUNEL assay on sections using an ApopTag in situ apoptosis detection kit (Merck-Millipore).
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bioRxiv - Microbiology 2021Quote: Apoptotic cells in the lung were detected by immunohistochemistry using the ApopTag in situ apoptosis detection kit (EMD Millipore) following the manufacturer’s directions ...
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bioRxiv - Biochemistry 2021Quote: ... Pluripotency status of control and ABCF1-knockdown cells were analyzed using an alkaline phosphatase (AP) detection kit (EMD Millipore), or by RT-qPCR analysis of mRNAs purified using TRIzol reagent (Life Technologies).
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bioRxiv - Cancer Biology 2021Quote: ... TUNEL Assay detecting apoptotic cell death were performed with the ApopTag Fluorescein in situ apoptosis detection kit (S7110, Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: PLA experiments were done using Duolink™ II secondary antibodies and detection kits (Sigma-Aldrich, #DUO92002, #DUO92004, and #DUO92008) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2020Quote: ... Absence of mycoplasma in cell lines was routinely checked by the VenorGeM Mycoplasma Detection Kit (Sigma, St. Louis, MO).
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bioRxiv - Neuroscience 2023Quote: ... It was further processed for the detection of glutamate release using glutamate determination kit (Sigma Aldrich, USA, Cat# GLN1) as per manufacturer’s protocol.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cell lines were verified to be free of mycoplasma contamination by Venor™ GeM Mycoplasma Detection Kit from Sigma.
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bioRxiv - Neuroscience 2024Quote: ... A fluorometric intracellular ROS detection kit was used to detect ROS levels at 30 and 120 minutes (Sigma, MAK144) using a Varioskan microplate reader (ThermoFisher).
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bioRxiv - Cell Biology 2023Quote: ... TUNEL staining was made according to the manufacturer’s instructions of the apoptosis detection kit in situ red (S7165, Merck-Millipore).
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bioRxiv - Molecular Biology 2023Quote: PLA experiments were done using Duolink™ II secondary antibodies and detection kits (Sigma-Aldrich, #DUO92002, #DUO92004, and #DUO92008) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... PLA probe maker kit and Duolink® in situ detection reagents were procured from Millipore Sigma (Burlington, MA, USA). RPMI medium 1640 was supplemented with 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... All cell lines were certified to be free of mycoplasma contamination using the Venor GeM Mycoplasma Detection Kit (Sigma) prior to experimental data collection.
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bioRxiv - Neuroscience 2023Quote: ... TUNEL staining was performed in a set of sections using the ApopTag in situ apoptosis detection kit (S7165, Millipore). Sections were visualized under confocal microscopy (Nikon) ...
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bioRxiv - Microbiology 2024Quote: Cell viability was assessed using the lactate dehydrogenase (LDH) enzyme activity method (Cytotoxicity Detection Kit version 11; Sigma-Aldrich) to quantify compromised cells in response to exposure to antibiotics ...
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bioRxiv - Neuroscience 2024Quote: ... The proximity ligation reaction and visualization of signal was performed according to the manufacturer’s protocol using the Duolink Detection Kit with PLA PLUS and MINUS probes for rabbit and human antibodies (Sigma). DAPI stain was used to detect cell nuclei ...
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bioRxiv - Immunology 2023Quote: ... or LIVE/DEAD™ Fixable Blue Cells Stain Kit (#L34957, Thermo; 1:1000 in PBS) and fixation for 15 minutes at RT using 1.85% formaldehyde (F1635, Sigma) in PBS solution before surface staining with antibodies in PBS supplemented with 0.5% BSA [fraction V ...
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bioRxiv - Molecular Biology 2019Quote: ... followed by 3X washes with 2XSSC and 30 min incubation in 2X SSC + 0.1% Tween20 (2XSSCT) + 50% (v/v) formamide (EMD Millipore, cat#S4117). For each sample ...
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bioRxiv - Bioengineering 2021Quote: ... for 12 days in SSC expansion media with the following additions: 100 ng/mL follicle stimulating hormone from human pituitary (FSH, Millipore Sigma, F4021), 10 ng/mL luteinizing hormone from human pituitary (LH ...
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bioRxiv - Bioengineering 2022Quote: ... we first mixed together all the secondary probes with 10% hybridization buffer with Triton-X (10% formamide, 10% dextran sulfate, 2X SSC, and 0.1% Triton-X (Sigma-Aldrich, T8787-100ML)) to a final ~20nM concentration per probe (range ...
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bioRxiv - Physiology 2022Quote: ... Dead cells and doublets were excluded on the basis of FSC and SSC distribution and DAPI exclusion (Sigma-Aldrich, catalogue number D9542) exclusion ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by 3X washes with 2XSSC and 30 min incubation in 2X SSC + 0.1% Tween20 (2XSSCT) + 50% (v/v) formamide (EMD Millipore, cat#S4117). For each sample ...
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bioRxiv - Genomics 2022Quote: Samples were then co-stained with 5ng/ml DAPI for 15 mins and imaged in an anti-bleaching buffer (50mM Tris-HCl, pH=8.0, 300mM NaCl, 2×SSC, 3mM Trolox (Sigma-Aldrich, Cat. 238813), 0.8% D-glucose (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by 3X washes with 2XSSC and 30 min incubation in 2X SSC + 0.1% Tween20 (2XSSCT) + 50% (v/v) formamide (EMD Millipore, cat#S4117). For each sample ...
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bioRxiv - Cell Biology 2024Quote: ... cells were deproteinated for 15 minutes in 0.1 N HCl and neutralized for 5 minutes in 2X Saline Sodium Citrate buffer (2X SSC) (Sigma Aldrich, Cat # S6639) at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... 5x SSCT buffer was prepared by diluting 20x SSC (saline sodium citrate) in DEPC water and adding 0.1% tween (Sigma Aldrich, Cat# P1379). Next ...
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bioRxiv - Biophysics 2023Quote: ... cells in each well were incubated with 100 µL of hybridization buffer (2× SSC, 50% formamide and 10% w/v dextran sulfate (Sigma, D8906-50G) mixed with ∼1 µM total primary probes ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by an over night incubation at 37°C in a slide hybridizer machine (ACD HybEZ™ II Hybridization System) with hybridization buffer (10% deionized formamide; 2X SSC; 10% w/v Dextran sulfate, Sigma-Aldrich, D8906 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... was purified using the GeneElute PCR Clean-Up Kit (Sigma-Aldrich, USA), then labeled with biotin-16-dUTP (Roche ...
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bioRxiv - Microbiology 2022Quote: ... Mice were genotyped using Extract-N-Amp Tissue PCR Kit (Sigma Aldrich) according to manufacturers’ instructions with the following primers ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we purified both libraries using the GenElute PCR Clean-Up Kit (Sigma) to remove short library fragments ...
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... Mice were genotyped using Extract-N-Amp Tissue PCR Kit (Sigma Aldrich) according to manufacturer instructions with the following primers ...
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bioRxiv - Physiology 2022Quote: ... and purified with the GenElute™ PCR Clean-Up Kit (SIGMA NA1020). 300 ng of the eluted DNA was digested overnight at 37°C with the restriction enzymes including USP8 – BstBI and USP48 – SpeI ...
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bioRxiv - Neuroscience 2023Quote: ... and PCR amplification was performed using KAPA2G ReadyMix Kit (Sigma Aldrich, KK5103) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: PCRs were performed with the KAPA3G Plant kit (Sigma Aldrich, MO, USA) using the following conditions ...
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bioRxiv - Cell Biology 2022Quote: ... for 1h at RT, blocked for 1h at RT in blocking solution (0.3% Triton X-100, 0.2% BSA (A4503, Sigma), and 5% goat serum (005-000-121 ...
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bioRxiv - Biochemistry 2023Quote: ... Affinity purified FH-Ago2 bound with miRNA (most population bound with miR-122 due to its overexpression) was eluted from anti-FLAG beads by competing with 3X FLAG peptide (Sigma) as per the manufacturer’s protocol in a purification buffer (30mM HEPES pH 7.4 ...
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bioRxiv - Molecular Biology 2020Quote: ... with Duolink Detection Reagent Green (Sigma, DUO92014) or Orange (DUO92007) ...
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bioRxiv - Synthetic Biology 2020Quote: ... and visualized using chemiluminescence detection (Immobilon, Millipore). Coomassie brilliant blue staining was conducted using Bullet CBB Stain One Super (Nacalai ...
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bioRxiv - Cell Biology 2021Quote: ... Protein detection using anti-HA (H9658, Sigma) or anti-Scp160 antibodies (Gift of R ...
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bioRxiv - Cell Biology 2021Quote: ... Detection was performed using TMB T0565 (Sigma).
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bioRxiv - Cell Biology 2020Quote: ... Detection was by chemiluminescence with luminol (Sigma) on BioMaxMR film (Kodak) ...
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bioRxiv - Cancer Biology 2022Quote: ... Enhanced chemiluminescence substrate detection system (Millipore-Sigma) was applied to detect bound antibody complexes and visualized by autoradiography ...
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bioRxiv - Cancer Biology 2022Quote: ... Enhanced chemiluminescence substrate detection system (Millipore-Sigma) was applied to detect bound antibody complexes and visualized by autoradiography ...
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bioRxiv - Molecular Biology 2020Quote: ... and In situ Detection Reagents Orange (Sigma) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2020Quote: ... following detection using PNPP substrate (Sigma, Israel).
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bioRxiv - Immunology 2022Quote: ... Staining with AldeRed ALDH detection assay (Sigma) was done following manufacturer’s recommendation with minor modifications ...
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bioRxiv - Cell Biology 2024Quote: ... Detection was examined with chemiluminescent substrate (Millipore) using the manufacturer’s protocols.
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bioRxiv - Molecular Biology 2023Quote: ... and chemiluminescent detection (ThermoFisher Scientific, Millipore WBKLS0500) were used to develop immunoblots ...
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bioRxiv - Immunology 2022Quote: ... and fixed in RT methanol (Sigma-Aldrich) for 2 minutes prior to PAS staining according to manufacturer’s protocol (Sigma-Aldrich ...