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Citations for Millipore Sigma :
9001 - 9050 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... H3K27me3 or HK3K27Ac ChIP-grade antibodies and Protein A magnetic beads (Millipore). FAIRE was performed on samples fixed and sonicated as per ChIP ...
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bioRxiv - Biochemistry 2023Quote: ... Antibody precipitated with 100 µL Protein A/G beads (Millipore, IP05-1.5ML) was washed twice in 700 µL DRIP buffer ...
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bioRxiv - Cell Biology 2023Quote: His-tagged recombinant proteins were expressed in Rosetta2 (DE3)pLysS bacteria (Novagen) and purified on IMAC columns (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: Equal amounts of protein samples were supplemented with β-mercaptoethanol (Sigma; M6250) and diluted in 1X Laemmli Sample buffer (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... Polyclonal antibodies from antiserum were purified using Protein A Sepharose (Sigma, USA) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were incubated with 20 μl protein G-coated Sepharose (Sigma-Aldrich) previously conjugated with 5 μl of anti-FLAG antibody by overnight incubation ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were detected by immunoblotting using anti-Glutathione-S-Transferase (Sigma-Aldrich), anti-polyHistidine-Peroxidase (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... Protein detection were performed with Immobilon Western Chemiluminescent HRP Substrate (ECL, Millipore). Band intensities were analyzed by ImageJ software.
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bioRxiv - Biophysics 2023Quote: ... The protein was further concentrated by a 100-kDa cutoff concentrator (Millipore) and loaded onto a Superose 6 increase 10/300 column (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... equal amounts of proteins were then incubated with anti-Flag Beads (Sigma), anti-Myc Beads (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... and the protein pellet resuspended and incubated in 2M hydroxylamine (NH2OH) (Sigma), pH 7.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were precipitated with 5x ice cold acetone (HPLC grade, Sigma-Aldrich) and centrifuged at 20,627 g for 10 min at 4° C ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were visualised using Immobilon Classico Western HRP substrate (Sigma-Aldrich, WBLUC0100). Primary antibody dilutions are as follow ...
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bioRxiv - Neuroscience 2023Quote: ... The dialyzed protein solution was filtered (0.45 μm pore size filter, Millipore) and protein concentration was determined by absorbance measurements at 280 nm using extinction coefficient ε = 37752 M-1cm-1 ...
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bioRxiv - Neuroscience 2023Quote: ... The dialyzed protein solution was filtered (0.45 μm pore size filter, Millipore) and protein concentration was determined by absorbance measurements at 277 nm using extinction coefficient ε = 5840 M-1cm-1 ...
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bioRxiv - Microbiology 2023Quote: ... Human interferon-β recombinant protein was purchased from Millipore (St. Louis, MO), and for stimulation ...
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bioRxiv - Microbiology 2023Quote: ... Elution of bound proteins was performed with 500 µL FLAG-peptide (Sigma) solution (100 ng/µL ...
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bioRxiv - Plant Biology 2023Quote: ... Crude protein concentration was determined using the Bradford Reagent (Sigma-Aldrich, MO), following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 25μg protein lysate were resuspended in Laemmli buffer 1X (Sigma, S3401-1VL), denatured at 95°C for 5 minutes and loaded onto a polyacrylamide gel (Mini-PROTEAN(R ...
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bioRxiv - Physiology 2023Quote: ... Protein concentration was qualified by Bradford assays (Sigma-Aldrich, B6916-500 mL). 30 μg soluble proteins were resolved on a 15% sodium dodecyl sulphate poly acrylamide gel via electrophoresis (SDS-PAGE ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein pellet was washed with −20°C cold acetone (Sigma-Aldrich), vortexed ...
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bioRxiv - Microbiology 2023Quote: ... the protein-DNA complexes were immunoprecipitated with an anti-FLAG antibody (Sigma) and Protein A sepharose beads ...
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bioRxiv - Cell Biology 2023Quote: ... or Glutathione High Capacity Magnetic Agarode Beads (for GST-tagged proteins, SIGMA). After 2 extensive washing with binding buffer ...
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bioRxiv - Cancer Biology 2023Quote: Immunoprecipitation was performed using Protein-A Sepharose from Staphylococcus aureus (Merck Millipore) and anti-TKS4 antibody (Lányi et al ...
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bioRxiv - Cell Biology 2023Quote: ... Transferred proteins were then stained with Ponceau S Solution (Sigma-Aldrich P3504) before being scanned on a flatbed scanner (Epson V800 Photo) ...
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bioRxiv - Microbiology 2023Quote: ... FLAG-tagged protein signals were detected with anti-FLAG (Sigma-Aldrich, F1804) in combination with anti-mouse secondary antibody (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... Protein concentration was measured using a Bradford assay (Sigma France, Lezennes, France). For SDS-PAGE analysis of purified proteins ...
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 spike protein (S1) (#E5S3V) and GAPDH (G9545, Sigma-Aldrich) were incubated overnight at 4 °C in TBS-T ...
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bioRxiv - Cancer Biology 2023Quote: ... protein samples were prepared with sample buffer that contains 10% glycerol (Sigma) and 5% 2-mercaptoethanol (Sigma) ...
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bioRxiv - Developmental Biology 2023Quote: ... Subsequently protein thiol groups were blocked with 10mM iodoacetamide (Sigma-Aldrich, USA) at RT for 45 min ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were concentrated to approximately 3 mg/ml by ultrafiltration (Millipore, UFC905096) at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Purified protein was then concentrated to 40mg/mL by centrifugal concentration (Millipore).
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bioRxiv - Biochemistry 2023Quote: ... The His-tag of the eluted protein was cleaved by thrombin (Sigma), incubated at 4 °C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were concentrated to >20 mg/mL on a Centricon device (Millipore).
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bioRxiv - Biochemistry 2023Quote: SYPRO Orange Protein Gel Stain (Cat#S5692, Sigma-Aldrich, St. Louis, USA) were diluted 1:2500 into the assay buffer (150 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... proteins entrapped in gel bands were reduced with 10 mM dithiothreitol (Sigma) at 60°C for one hour and then alkylated with 50 mM iodoacetamide (Sigma ...
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bioRxiv - Biophysics 2023Quote: ... The eluted protein was concentrated with a 100kDa-cutoff spin concentrator (Millipore), after which 1:10 (w/w ...
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bioRxiv - Cell Biology 2023Quote: ... Protein synthesis was inhibited by adding cycloheximide (CHX) (Sigma, catalog number: 01810) at a final concentration of 100 µg/mL ...
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bioRxiv - Genetics 2022Quote: ... Eluted proteins were loaded on the centrifugal filter unit (Millipore-Sigma, UFC8030) and centrifuged at 3,500 g 4°C for 20 minutes to eliminate any remaining maltose from the elute ...
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bioRxiv - Bioengineering 2022Quote: ... Protein concentration was quantified by Coomassie staining using BSA standards (Sigma P0914).
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bioRxiv - Genomics 2023Quote: ... protein stability was assessed by adding 5 mg/ml cycloheximide (Sigma-Aldrich) to the cell growth medium for the indicated times prior to harvest ...
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bioRxiv - Genetics 2022Quote: ... Eluted proteins were loaded on the centrifugal filter unit (Millipore-Sigma, UFC8030) and centrifuged at 3,500 g 4°C for 20 minutes to eliminate any remaining maltose from the elute ...
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bioRxiv - Cell Biology 2023Quote: ... Biotinylated proteins were directly labeled using alkaline phosphatase-coupled streptavidin (Sigma-Aldrich). The blots were developed in a solution of nitroblue tetrazolium chloride (NBT ...
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bioRxiv - Cell Biology 2022Quote: ... The protein bands were visualized by an ECL detection system (EMD Millipore).
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bioRxiv - Cell Biology 2023Quote: ... Whole-cell protein lysates were prepared in CelLytic M lysis buffer (Sigma) with 1% protease/phosphatase inhibitors (Sigma ...
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bioRxiv - Biophysics 2023Quote: ... Cell lysates were incubated with Protein G sepharose beads (Sigma-Aldrich P3296) with anti-RpoC antibody (Biolegend clone NT73 ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then filtered through a 10kDa protein filter (MRCPRT010, Sigma Aldrich) via centrifugation at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... The dialyzed protein was concentrated using a 30 kDa MWCO concentrator (Millipore) and was further purified by size-exclusion chromatography on a Superose 6 Increase 10/300 GL column (Cytiva ...
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bioRxiv - Genetics 2023Quote: ... the Cas9 protein was expressed in BL21 Rosetta Escherichia coli strain (Novagen) in magic medium at 37 °C for 12 h followed by 24 h at 18 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Bound proteins were eluted in Flag peptide (Sigma, F4799, 150 ng/μl) for 30 minutes ...