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Citations for Millipore Sigma :
9201 - 9250 of 10000+ citations for Mouse TIG2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... after which bone marrow cells were collected and labeled with monoclonal anti-mouse CD3 (Sigma, catalog no. SAB4700050, 1: 1000), CD19 (Sigma ...
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bioRxiv - Genetics 2021Quote: ... were superovulated by serial Pregnant Mare Serum Gonadotropin (5 IU per mouse, Prospec Protein Specialists) and human chorionic gonadotropin (5 IU, Millipore) injections 46 h apart ...
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bioRxiv - Immunology 2020Quote: ... then binding was detected with specific alkaline phosphatase-conjugated secondary antibodies (anti-mouse total IgG and anti-monkey total IgG from Sigma and anti-mouse IgG1 and IgG2a from BD Biosciences) ...
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bioRxiv - Neuroscience 2020Quote: ... The mouse anti-GFP-20 antibody was used in GRASP and synaptotagmin experiments (1:100 in PBST-NGS; Sigma-Aldrich); the mouse nc82 antibody was used in synaptotagmin::GFP and DenMark experiments ...
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bioRxiv - Microbiology 2020Quote: ... lysates were pre-cleared for 1 hour (h) with mouse immunoglobulin G (IgG) agarose or rabbit immunoglobulin G (IgG) agarose (Sigma), dependent on the species of antibody used in IP ...
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bioRxiv - Immunology 2020Quote: ... 8-µm-thick tissue sections were rehydrated and Fc-blocked with normal mouse and rat serums in the presence of 0.3% Triton X100 (Sigma-Aldrich). Tissues were stained with F4/80-APC (Clone BM8 ...
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bioRxiv - Molecular Biology 2021Quote: Total splenocytes were isolated from C57BL/6 and mFICD-/- mouse spleens and then treated for 3 days with 20 μg/ml LPS (Sigma), 100 µg/ml heparan sulfate or 2.5 μM thapsigargin (Enzo Life Sciences) ...
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated with mouse monoclonal Alexa Fluor-488 conjugated antibody against NeuN (1:200, MAB377X, Merck Millipore, MA, USA) or the rabbit polyclonal primary antibody against DISC1 (1:250 ...
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bioRxiv - Physiology 2020Quote: ... The presence of LPL in the conditioned media was confirmed through Western blotting using a mouse antibody against the FLAG tag (1:5000; Sigma). LPL activity was assessed through an LPL activity assay (see below).
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bioRxiv - Immunology 2021Quote: ... the purified MECU protein was separated on 12% SDS-PAGE and further probed with mouse polyclonal anti-His (Sigma, USA) and rabbit polyclonal anti-H ...
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bioRxiv - Immunology 2020Quote: ... for 1h at RT or addition of AP-conjugated goat anti-mouse IgM or IgA (Abcam and Sigma-Aldrich, respectively) and addition of pNPP substrate (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: Total RNA from human hippocampal and prefrontal cortex tissue and mouse hippocampal tissue was extracted with TRIzol reagent (Sigma-Aldrich) and 1 μg was reverse-transcribed using PrimeScriptTM RT Reagent Kit (perfect Real Time ...
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bioRxiv - Neuroscience 2020Quote: ... SI and protein extracts from human and mouse samples were resolved in SDS-PAGE transferred to Immobilon-P PVDF membranes (Millipore). Membranes were activated with methanol and blocked with 4 % bovine serum albumin (BSA ...
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bioRxiv - Genetics 2020Quote: ... Cells were incubated with rabbit anti-TMEM43 polyclonal antibody (1:100, NBP1-84132, Novus) (Ab1-2) and mouse anti-FLAG (1:500, F1804, Sigma) for overnight ...
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bioRxiv - Cell Biology 2021Quote: ... Commercial antibodies used for immunofluorescence included mouse anti-neurofilament (1:200, clone SMI-312, #837904) and mouse anti-βIII-tubulin (1:100, clone TU-20, #MAB1637) from Millipore-Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... The flowthrough containing the mouse MLKL(1-158) construct was concentrated via centrifugal ultrafiltration (10 kDa molecular weight cut-off; Millipore) and loaded onto a Superdex 75 Increase 10/300 GL size exclusion column (Cytiva ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were placed in a light-protected humidity-controlled tray and incubated in primary antibody (mouse-anti-Neurofilament 200; clone N52; Sigma) at 1:500 in blocking buffer overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... and with 10mM EDTA then incubated with Goat anti plexin-A2-APC (RαD systems) for 40 min in the dark at 25 °C for staining plexin-A2 or with Mouse anti-FLAG (Sigma) for 40 min at 4°C followed by Goat anti mouse IgG H&L (Alexa Fluor®647)(abcam ...
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bioRxiv - Genetics 2020Quote: ... 4 RIMBP1 WT and 4 RIMBP1 KO cerebelli were cut parasagittal (30 um) and stained with antibodies anti-GAD65 (1:1000, mouse, DSHB) and anti-calbindin (1:1000, rabbit, Millipore). Synaptic puncta were imaged using a Nikon confocal microscope (A1RSi+ ...
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bioRxiv - Genetics 2020Quote: Western blotting of RPB-9 in the rpb-9::ollas line (Figure S4B) was performed using a mouse anti-tubulin (clone DM1A, SIGMA) antibody and a rat anti-ollas (NBP1-06713 ...
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bioRxiv - Cell Biology 2021Quote: ... The following antibodies were used for immunoblotting: The HA epitope was recognised with a primary mouse monoclonal anti-HA (1:1000; Sigma); the Myc epitope was recognised with a primary mouse monoclonal anti-Myc (1:5000 ...
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bioRxiv - Biophysics 2021Quote: ... The following day (day 0) the medium was changed to mouse embryonic fibroblasts (MEF) medium containing doxycycline (2 ng/ml, Sigma) to initiate the expression of the transgenes and thus ...
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bioRxiv - Cell Biology 2021Quote: ... Immunohistochemistry of frozen sections was carried out via standard techniques with α-Tubulin (T5168, mouse monoclonal, Sigma; diluted 1:1000) and a secondary conjugated antibody at room temperature for 2 hours ...
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bioRxiv - Neuroscience 2020Quote: ... Hippocampi were isolated from mouse embryos (E18) or newborn pups (P0) and digested with 0.25% trypsin-EDTA (1x) (Sigma, T4549) at 37°C for 15 minutes ...
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bioRxiv - Neuroscience 2022Quote: Primary cultures of mouse astrocytes were grown in vitro in the plates coated with 5 μg/mL fibronectin (Sigma, F2006) for 4-5 days before they were infected with lentivirus ...
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bioRxiv - Immunology 2022Quote: ... and the secondary antibody goat anti-mouse IgG (H+L) conjugated to Alexa Fluor 488 were obtained from Millipore Sigma. Monoclonal Rac1 antibodies (Cat ...
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bioRxiv - Molecular Biology 2022Quote: ... 250 μg of total protein samples were made up to 500 μl total volume with 1% NP-40 buffer and incubated overnight at 4°C with 1 μl of mouse anti-Flag primary antibody (#F1804; Sigma). Subsequently ...
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bioRxiv - Molecular Biology 2022Quote: ... mouse anti-γ-H2AX JBW301 (1:1000 for IB, 1:2500 for IF, 1:5000 for FACS; Millipore, 05-636), mouse anti-γ-H2AX-Alexa Fluor 647 (1:1000 for FACS ...
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bioRxiv - Cancer Biology 2022Quote: ... The primary antibodies used were as follows: mouse anti-phospho-Histone H2A.X (Ser139; clone JBW301; Sigma, 05-636; 1/1,000), mouse tubulin (clone B-5-1-2 ...
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bioRxiv - Biochemistry 2022Quote: ... Flag-tagged H2B was detected with western blot using mouse anti-FLAG (F3165, Sigma-Aldrich, RRID: AB_259529, 1:3000 diluted) and HRP-conjugated anti-mouse (sc-516102 ...
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bioRxiv - Cancer Biology 2022Quote: ... diluted 1:1000 in casein and incubated overnight at 4 degrees Celsius with shaking OR Monoclonal Anti-Beta-Actin mouse antibody (Sigma) diluted 1:5000 in casein and incubated at room temperature for 1 hour ...
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bioRxiv - Biophysics 2022Quote: ... the acini were incubated with the primary antibody mouse anti-podocalyxin (EMD Millipore, clone 3F2:D8, working dilution 1:200), mouse β1 integrin (Invitrogen ...
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bioRxiv - Biophysics 2022Quote: ... The pre-cleared lysates were then incubated with 100 µL of antibody/protein A complexed beads (anti-FLAG [mouse, Sigma, F1804] or anti-HA [mouse ...
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bioRxiv - Cell Biology 2022Quote: ... U2OS and SYF (src -/- yes -/- fyn -/-) mouse embryonic fibroblasts (Klinghoffer et al., 1999) were cultured at 37 °C with 5% CO2 in DMEM (Sigma) with 4.5 g/l L-glucose ...
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bioRxiv - Cell Biology 2022Quote: ... The mouse monoclonal anti- FLAG (catalog #: F1804) and anti-β-actin (catalog #: A1978) antibodies came from Sigma (St. Louis, MO). The mouse monoclonal anti-Hsp47 antibody (catalog # ...
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bioRxiv - Cell Biology 2022Quote: The following monoclonal antibodies were used for immunofluorescence: anti-acetylated tubulin (mouse; clone 6-11B-1; Sigma-Aldrich; 1:500), anti-ubiquitin clone FK2 (mouse ...
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bioRxiv - Genetics 2022Quote: ... Binding of the anti-FLAG M2 primary antibody (mouse, Sigma-Aldrich, F3165, diluted 1:3000 in PBS-T+1%BSA) was allowed in a humidity chamber (overnight ...
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bioRxiv - Developmental Biology 2022Quote: ... Naive hPSCs were grown either on irradiated MF1 mouse embryonic fibroblasts (MEFs) (Wellcome-MRC Cambridge Stem Cell Institute) on plates pre-coated with 0.1 %Gelatin (Sigma-Aldrich), or in feeder-free conditions using Geltrex Matrix (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Detection was carried out using anti-mouse IgG-HRP antibody (GE; 1:5000 in PBS + 2% BSA) followed by addition of TMB substrate (Sigma). Reaction was stopped by adding 0.5 M H2SO4 ...
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bioRxiv - Microbiology 2022Quote: ex-vivo mouse fecal experiments with sterile and non-sterile mouse fecal media were conducted aerobically at 37°C in 48 well plates (Sigma) with shaking (210 rpms) ...
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bioRxiv - Microbiology 2022Quote: ... the plates were washed 3 times with 1x PBS to remove the supernatant and the degradation of the pre-coated ECM components was detected using mouse anti-fibronectin (F7387, 1:5,000; Millipore Sigma), and rabbit anti-laminin (L9393 ...
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bioRxiv - Microbiology 2022Quote: ... samples were washed with 1 ml PBS buffer three times and vacuum-infiltrated for 20 min with 0.1 mg/ml of primary mouse anti-1,3-β-glucan antibody (Biosupplies; Sydney, Australia) for β-1,3-glucan labelling or 0.1 mg/ml mouse MOPC-104E primary antibody (Sigma-Aldrich) for α-1,3-glucan labelling ...
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bioRxiv - Pathology 2022Quote: ... The membranes were washed three times with Tris-buffered saline and Tween-20 followed by incubation with the peroxidase-conjugated anti-mouse or anti-rabbit secondary antibodies (both from Millipore) for 1 h at room temperature.
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bioRxiv - Neuroscience 2022Quote: ... Binding of the FLAG-tagged DARPins to streptavidin-immobilized biotinylated gephyrin variants was measured using a mouse-anti-FLAG-M2 antibody (Sigma) as 1st and goat-anti-mouse-alkaline phosphatase conjugated antibody (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were washed with TBS-T and membranes were incubated with secondary antibodies anti-mouse and anti-rabbit coupled to alkaline phosphatase (1:5000; Sigma) diluted in TBS-T/1% BSA for one hour at RT ...
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bioRxiv - Microbiology 2022Quote: ... RBD or spike-bound serum antibodies were detected with horseradish peroxidase conjugated goat anti-mouse IgG (1:500 dilution, Milipore Sigma) incubating for 2 h at 25°C ...
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bioRxiv - Neuroscience 2023Quote: ... we randomly assigned Ai9:Tmem119cre mice to receive an intravenous injection of control solution or 0.05 mg/kg of recombinant IFNγ from mouse (Sigma #I4777), minutes before initiating the rupture of cortical capillaries and then 12h later ...
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bioRxiv - Molecular Biology 2023Quote: ... HRP-conjugated anti-rabbit (catalog no. 401315) and anti-mouse (catalog no. 401215) secondary antibodies and BSA (catalog no. 12659) were from Millipore. All antibodies were diluted in 1% BSA dissolved in PBS-T (1.5 mM Sodium Phosphate Monobasic ...
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bioRxiv - Physiology 2023Quote: One kidney from each mouse was mechanically dissociated using scissors and digested with 0.156mg/ml Collagenase type XI (Sigma-Aldrich), 0.030mg/ml Hyaluronidase type IV-S (Sigma-Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... primary antibodies were incubated overnight at 4°C: mouse monoclonal anti-C5aR1 (clone: S5/1; Millipore; cat. MABF1980; 1:50), rabbit polyclonal anti-IBA1 (FUJIFILM Wako Pure Chemical Corporation ...