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Citations for Millipore Sigma :
9001 - 9050 of 10000+ citations for Mouse TIG2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... hybridization buffer contained 1:100 dilution of TERT exon and intron probes and 1:800 dilution of primary antibody (mouse M2 monoclonal anti-FLAG, F1804, Sigma), and (ii ...
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bioRxiv - Cell Biology 2020Quote: ... The resins were washed 3x with TBS-T at 4°C for 20 min and then separated by SDS-PAGE followed by Western blottings with mouse monoclonal anti-HA (12CA5, Sigma) or anti-myc (9E10 ...
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bioRxiv - Biophysics 2019Quote: ... incubated again for 1 hour at 37°C with 1:400 mouse anti-heavy chain clathrin antibody (Sigma Aldrich, C1860) in PBS + 1% BSA and washed three times with PBS + 1% BSA ...
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bioRxiv - Biophysics 2019Quote: ... the cells were incubated for 1 hour at room temperature with 1:300 mouse β-tubulin antibody (Sigma Aldrich, T5293) in PBS + 1% BSA ...
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bioRxiv - Microbiology 2019Quote: ... samples were then stained using Alexa 488-conjugated anti-mouse and Alexa 594-conjugaed anti-rabbit antibodies and DAPI (Sigma) for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... sections were incubated with 0.5% Triton and blocked with 0.5% Triton and goat serum blocking solution before being incubated in mouse anti-PV monoclonal antibody (1:2000, Sigma-Aldrich) and mouse anti-NeuN polyclonal antibody (1:2000 ...
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bioRxiv - Biophysics 2019Quote: ... fab-fragment solution consisting of 100 μg/ml F(ab’)2 fragments (anti-mouse IgG [Fc specific] antibody developed in goat; Sigma) in PBS that bind unspecifically to the hydrophobic surface (incubation time 5 min) ...
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bioRxiv - Physiology 2019Quote: ... anti-Rabbit secondary (Cat. No# A0545) and anti-mouse secondary (Cat. No# A9044) antibodies were obtained from Sigma Aldrich (USA).
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bioRxiv - Immunology 2019Quote: ... Secondary HRP-conjugated antibodies (polyclonal goat anti-mouse IgG at 1/1000 or anti-rabbit IgG at 1/2500 from Millipore) were used to detect antibody binding ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were incubated with primary antibodies in BB for 1h at RT: monoclonal mouse anti-Col4a2 (Millipore, Cat. No. MAB1910), polyclonal rabbit anti-LM (Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... The sections were collected in PBS in 24-well plates and processed for free-floating immunofluorescence using primary polyclonal antibodies that label neurons (NeuN, mouse; 1: 1000, Millipore MAB377, Merck Millipore), reactive astrocytes (GFAP ...
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bioRxiv - Cell Biology 2019Quote: ... After washing 3–4 X with 1× PBS-T the strips were incubated with 1:5000 mouse anti-GST antibodies (Sigma). The secondary antibodies (anti-rabbit-HRP or anti-mouse-HRP ...
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bioRxiv - Cell Biology 2019Quote: ... Western blots were performed using standard methods and the following antibodies: Mouse anti-FUS (AMAB90549 Sigma Aldrich, 1:500 dilution), rabbit anti-GFP (sc-8334 Santa Cruz ...
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bioRxiv - Molecular Biology 2019Quote: ... blots were subsequently incubated for 1h at room temperature with horseradish peroxidase-conjugated secondary anti-rabbit/mouse antibodies (1:10000; Sigma). Immunoreactive bands were visualized by chemiluminescence detection of peroxidase activity (SuperSignal West Dura ...
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bioRxiv - Cell Biology 2019Quote: ... Duolink® In Situ PLA Rabbit Plus and Mouse Minus probes were used in combination with Detection Reagent Green (Sigma) following manufacture’s protocol ...
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bioRxiv - Genomics 2021Quote: ... as well as two monoclonal anitbodies targeting the KMT2A C-terminus: Mouse anti-KMT2A (1:100, Millipore Cat #05-765) refered to as KMT2A-C1 ...
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bioRxiv - Cell Biology 2020Quote: ... 1999), rabbit anti-HTP-1/2 (1:500) (Martinez-Perez et al., 2008) and mouse anti-FLAG (1:200) (Sigma).
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bioRxiv - Cancer Biology 2021Quote: ... The DNA encoding MHC heavy chain (HLA-C*08:02 and H-2 Kb) and light chain (human β2M and mouse β2M) was synthesized (Idobio) and cloned into pET-22(b) vector (Novagen). The vectors were transformed into the E ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were diluted in 0.1 % Triton X-100 in PBS as follows: APP-CT (mouse, Millipore MAB343, 1:100), PML (rabbit ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Sections containing SN and VTA were incubated with antibodies directed against tyrosine hydroxylase (mouse monoclonal, 1:1000, Merck-Millipore MAB318) and mCherry (rabbit polyclonal ...
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bioRxiv - Neuroscience 2020Quote: ... GFAP (1:200, guinea pig, Synaptic Systems; 1:1000, mouse directly conjugated to Cy3, Sigma-Aldrich; 1:1000, chicken, Millipore), GFP (1:2000 ...
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bioRxiv - Neuroscience 2020Quote: ... GFAP (1:200, guinea pig, Synaptic Systems; 1:1000, mouse directly conjugated to Cy3, Sigma-Aldrich; 1:1000, chicken, Millipore), GFP (1:2000 ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibodies, anti-rabbit IgG-HRP (1:5000, #7074, CST) or anti-mouse-HRP (1:10000, #12-349, Merck Millipore) in TBST containing 5% milk for 1 h at RT ...
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bioRxiv - Microbiology 2021Quote: ... were resolved on a 12.5% SDS gel and puromycin-labelled peptides were detected with anti-puromycin (1:5000 mouse anti-puromycin, clone 12D10; Sigma). Prior to antibody detection ...
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bioRxiv - Neuroscience 2021Quote: ... Some sections were also incubated with a combination of a mouse monoclonal anti-GFAP antibody (1:1,000; G3893, Sigma-Aldrich) and the above-described polyclonal anti-GFAP antibody to verify whether both antibodies provided similar detections of GFAP protein ...
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bioRxiv - Cancer Biology 2020Quote: ... Total protein lysates obtained from transfected cells were used for Western Blot analysis using the following antibodies: anti-MYB mouse monoclonal (1:1000, Upstate/Millipore) and anti-GAPDH mouse monoclonal (1:10000 dilution ...
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Safety and efficacy of C9ORF72-repeat RNA nuclear export inhibition in amyotrophic lateral sclerosisbioRxiv - Systems Biology 2021Quote: ... cell cultures were incubated with the appropriate primary antibodies (rabbit anti-Nestin, 1:500 [Biolegend #841901]; mouse anti-Pax6, 1:200 [Millipore #MAB5552] ...
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Therapeutic base and prime editing of COL7A1 mutations in recessive dystrophic epidermolysis bullosabioRxiv - Bioengineering 2021Quote: Frozen skin tissues from a normal individual and the patients were sectioned at 6 μm and stained with mouse monoclonal anti-NC1 C7 antibody (clone LH 7.2; Sigma-Aldrich) at a 1:1000 dilution ...
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bioRxiv - Bioengineering 2021Quote: ... This was followed by two 10 min washes and a 1 h incubation in peroxidase-labelled anti-mouse antibody (1:2000, Sigma). Membranes were developed by homemade enhanced chemiluminescence and scanned using FluorChem M hardware (Proteinsimple).
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bioRxiv - Biochemistry 2021Quote: ... 1 mg of proteins was used to performed incubation overnight at 4°C with 5 μg of anti-Flag mouse antibody (Sigma) in 250 μL of complemented non-denaturing lysis buffer ...
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bioRxiv - Biochemistry 2021Quote: ... Pierce anti-HA beads for immunoprecipitation of HA-tagged GAPDH was purchased from Thermo Fisher (Cat. # 88836) Rabbit polyclonal antibody (Cat. # G4595) and mouse monoclonal antibody against GAPDH (Cat. # G8795) were purchased from Sigma Aldrich. Rabbit polyclonal antibody against Peroxiredoxin-SO3 (Cat ...
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bioRxiv - Neuroscience 2021Quote: ... for 30 min prior to incubation for 1 h in primary antibodies diluted in blocking buffer: Goat anti-mouse GAPDH (RRID: AB_1078991, Sigma-Aldrich) 1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... For analysis of human OL 3000-4000 cells were seeded on wells coated with 5-10 µg/ml mouse-laminin (#L2020, Sigma) and 0.025 % poly-L-lysine (#P4707 ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were further incubated with secondary antibodies (mouse, 1:10000 dilution, A9044, Sigma; rabbit, 1:10000 dilution, A9169, Sigma). Immunoreactive bands were visualized by ChemiDoc XRS+ imaging system ...
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bioRxiv - Neuroscience 2021Quote: ... GABAergic neurons were labeled using anti-parvalbumin mouse antibody (PARV-19, 1:1000; Sigma, St. Louis, MO, USA, Product# P3088). All primary antibodies were diluted in goat serum (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... The immunoblotting was performed with the following primary antibodies (overnight at 4°C) and secondary antibodies (1h at RT): mouse-anti-Flag (M2 monoclonal, #F3165, 1:3000, Sigma), rabbit-anti-GAPDH (monoclonal ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were then incubated in anti-ChAT mouse IgG1 primary antibody (1:2000 overnight at 4°C; Sigma-Aldrich) in solution with PBS and NGS ...
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bioRxiv - Neuroscience 2020Quote: ... FITCD was injected intravenously (1 mg/25 g mouse, 0.5% solution in 0.9% physiological saline, Sigma-Aldrich, St. Louis, USA) and allowed to circulate for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... nsp8 and nsp12 proteins were subsequently detected using the mouse monoclonal anti-DYKDDDDK M2 antibody (Catalogue number F3165, Sigma-Aldrich) diluted 1:1000 in TBST/5% milk ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated for 1 hr at room temperature with mouse antibody against α-tubulin (1:500; Sigma Aldrich, T6199), rabbit antibody against Arl13b (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... The cDNA sequence encoding 1053-1264 aa mouse was amplified and cloned upstream of sequences encoding a polyhistidine stretch (His) tag in pET23b (Novagen). The mGli2-His recombinant protein was expressed in BL21 (DE3 ...
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bioRxiv - Cell Biology 2021Quote: ... The following antibodies (at the indicated dilution) were used for this publication: monoclonal mouse anti-Flag (1:2,000) (M2, Sigma F1804), and monoclonal mouse anti-α-tubulin (1:5,000 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the slides were incubated for 1 h at room temperature with the appropriate biotin-conjugated secondary antibody: goat anti-mouse (21538, Merck-Millipore) for CD3 and goat anti-rat (BP-9400 ...
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bioRxiv - Molecular Biology 2020Quote: ... transferred to nitrocellulose membrane and probed with primary antibodies against HA (HA-7 mouse monoclonal, 1:2000 dilution, Sigma; or rabbit polyclonal, 1:1000 dilution, Sigma), α-tubulin (mouse monoclonalDM1A ...
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bioRxiv - Physiology 2021Quote: ... Samples were analyzed using a rat insulin radioimmunoassay that has 100% cross-reactivity for mouse insulin (Millipore-Sigma #ri-13k). Insulin content was measured after acid-ethanol extraction using an insulin ELISA (Stellux Rodent Insulin ELISA ...
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bioRxiv - Physiology 2021Quote: ... Samples were analyzed using a rat insulin radioimmunoassay that has 100% cross-reactivity for mouse insulin (Millipore-Sigma #ri-13k). Insulin content was measured after acid-ethanol extraction using an insulin ELISA (Stellux Rodent Insulin ELISA ...
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bioRxiv - Physiology 2020Quote: The membrane was washed in TBST and incubated with a peroxidase-conjugated secondary antibody (goat, anti-mouse IgG, Sigma-Aldrich) diluted 1 ...
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bioRxiv - Cell Biology 2021Quote: ... The blot was blocked in 5% Milk in tris-buffered saline with 0.1% Tween 20 (TBST) and then incubated in 5% Milk TBST containing mouse anti-DPYD (Sigma, WH0001806M1) and rabbit anti-β-actin (Cell Signaling ...
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bioRxiv - Microbiology 2020Quote: ... Supernatants were submitted to the UIC Flow Cytometry Core in order to carry out a 32-plex cytokine/chemokine analysis using the 32-plex Milliplex Map Mouse Cytokine/Chemokine Magnetic Bead Panel (Millipore). Results were compared between WT and Δrgg2 or WT and Δrgg3 by applying a student’s t test (p=0.05).
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bioRxiv - Microbiology 2020Quote: ... Serial dilutions of mouse sera were tested for binding to the biotinylated proteins and the bound antibodies detected using HRP-conjugated anti-mouse IgG A4416 (Sigma) and TMB substrate (Life Technologies).