Labshake search
Citations for Millipore Sigma :
9351 - 9400 of 10000+ citations for Mouse TIG2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... and mouse BRCA2 (amino acid residues: 2232-2283) were cloned into pMAT11 (Peranen et al., 1996) and pRSF-Duet1 (Merck Millipore) vectors for expression with an N-terminal TEV-cleavable His6-MBP tag and an N-terminal TEV-cleavable MBP tag ...
-
bioRxiv - Cancer Biology 2023Quote: Antibodies used in this study are as follows: mouse monoclonal anti-β-tubulin antibody (Sigma-Aldrich, Cat# T4026, 1:5000), mouse monoclonal anti-FLAG M2 antibody (Sigma-Aldrich ...
-
bioRxiv - Plant Biology 2023Quote: ... as primary antibodies and Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L) (115-035-003, Jackson) or Peroxidase Conjugated Goat Anti-Rabbit IgG (H+L) (AP132P, Sigma) as secondary antibodies ...
-
bioRxiv - Systems Biology 2023Quote: ... All membranes were blocked in a 5% milk solution and then incubated for 12h at 4°C with either the mouse anti-phosphotyrosine 4G10 (05321, Millipore) or the rabbit anti-GFP (Thermo A11122 ...
-
bioRxiv - Cell Biology 2023Quote: ... mouse monoclonal anti-PP2A C-subunit clone 1D6 conjugated with Alexa Fluor 488 (Sigma-Aldrich, 05-421-AF488, 1:50). Anti-human Cy3 (Jackson ImmunoResearch ...
-
bioRxiv - Genomics 2023Quote: ... Expression of the Dam-POI in the mouse ESC lines was suppressed by addition of 0.5 mM indole-3-acetic acid (IAA; Sigma, I5148).
-
bioRxiv - Cell Biology 2023Quote: ... and incubated as described above with a 0.5 μg/ml mouse α-tubulin antibody (clone B-5 1-2, Sigma) as a loading control.
-
bioRxiv - Microbiology 2023Quote: ... were also challenged with mouse peritoneal macrophages (Nrf2 +/+) or (Nrf2 -/-) pre-treated or not with ML385 at 10µM (Sigma-Aldich) or h-MDM pre-treated with ML385 at 10µM (Sigma-Aldich) ...
-
bioRxiv - Synthetic Biology 2023Quote: Stable cell lines expressing human or mouse PRLR were generated by lentiviral transduction followed by 0.7 ug/mL puromycin selection (Sigma; P9620). 12ug of each pTwist Lenti Puro SFFV WPRE lentiviral construct encoding either human or mouse PRLR were co-transfected with 9ug of psPAX2 (Addgene ...
-
bioRxiv - Molecular Biology 2023Quote: ... CRHR2α expression levels in the stably transfected cell lines was assessed by Western blot using a mouse monoclonal anti FLAG -antibody (1:5000 dilution; SIGMA). All the experiments were performed at least 3 times with 3 individual clones (clones 4 ...
-
bioRxiv - Neuroscience 2023Quote: ... brain homogenate from a RML inoculated C57BL6/J mouse at the terminal phase of disease was treated with 100 μg/ml of pronase E (Sigma) for 30 min at 37 °C with shaking in a thermal mixer at 800 rpm ...
-
bioRxiv - Neuroscience 2023Quote: ... diluted in PBS for 1 h before immunoreaction with the following antibodies: mouse anti-GFAP (1:1,000; Millipore, Cat. MAB360), rabbit anti-GFAP (1:1,000 ...
-
bioRxiv - Microbiology 2023Quote: ... incubated for 1 h at room temperature with an anti-mouse IgG secondary antibody conjugated with peroxidase (Sigma Aldrich, A9044) diluted 1:10,000 in blocking buffer ...
-
bioRxiv - Immunology 2023Quote: ... 8 mg of purified anti-FH OX23 antibody (MRC Immunochemistry Unit, Oxford) from mouse hybridoma cell line culture supernatant was immobilised on CNBr-activated Sepharose (Sigma) (2 mg/ml packed Sepharose) ...
-
bioRxiv - Molecular Biology 2023Quote: ... isolated macular retinal nuclei were stained with a mouse anti-NeuN monoclonal antibody (1:5000, Alexa Flour 488 Conjugate MAB377X, Millipore, Billerica ...
-
bioRxiv - Cancer Biology 2023Quote: The formation of DNA damage-induced γ-H2AX foci was analyzed by Immunofluorescence staining using a mouse monoclonal anti-phospho-Histone H2A.X (Ser139) (Sigma) as described before 40 ...
-
bioRxiv - Cancer Biology 2023Quote: ... PLA reactions were subsequently carried out using Duolink PLA plus and minus probes for rabbit and mouse (respectively) and Duolink In Situ Detection Reagents Orange (Sigma) following the manufacturer’s protocol ...
-
Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: Mouse cortices from embryonic day 18 (E18) embryos were dissected into ice-cold HBSS and dissociated in acetylated trypsin (Sigma) for 20 min at 37°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... sub-confluent mouse embryonic 3T3-J2 fibroblasts were treated for 2 hours with 4µg/ml Mitomycin C (Sigma-Aldrich, M4287), washed three time with PBS ...
-
bioRxiv - Biochemistry 2023Quote: ... The protein bands were obtained using horseradish peroxidase-conjugated antibodies against mouse whole immunoglobulins and horseradish peroxidase-conjugated antibodies against rabbit whole immunoglobulins (1∶10,000, Sigma-Aldrich) as secondary antibodies and detection with the Lumi-Light Plus Western Blotting Substrate (Roche) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Resulting immunocomplexes were detected by HRP-conjugated anti-mouse IgG or anti-rabbit IgG secondary antibodies and enhanced chemiluminescent (ECL) reagents (WBLUF0100, Merck Millipore).
-
CRISPR-Cas9 Engineered Extracellular Vesicles for the Treatment of Dominant Progressive Hearing LossbioRxiv - Bioengineering 2023Quote: Genomic DNA was extracted from mouse pinna by incubating ear punches with 40μL tissue preparation solution (Sigma, Cat No. T3073) and 40μL extraction solution (Sigma ...
-
bioRxiv - Cell Biology 2023Quote: ... supplemented with 20 ng/mL mouse GM-CSF (Miltenyi #130-095-739) and 0.5 μM β-estradiol (Sigma-Aldrich #E2758)] and transduced with 1:5 (vol:vol ...
-
bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies coupled to horseradish peroxidase (HRP) were anti-rat-HRP at 1:10000 and anti-mouse-HRP at 1:10000 (Sigma).
-
bioRxiv - Cell Biology 2023Quote: ... The following antibod-ies were used for western blots of human cell extracts: Mouse anti-FLAG (Sigma F1804, 1:1000); Mouse anti-GAPDH (Proteintech 60004-1-Ig ...
-
bioRxiv - Microbiology 2023Quote: ... the membrane was further incubated 1 hour at 25°C with a second antibody (anti-mouse HRP conjugated antibody; Sigma) diluted 1:10,000 in PBS-Tween ...
-
bioRxiv - Immunology 2024Quote: ... mice were bled for serum cytokine analysis using MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel (Millipore MCYTOMAG-70K-PMX) according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... Slides were then washed twice with PBS and processed using Duolink Proximity Ligation Assay (PLA) reagents (In-Situ PLA Probe Anti-Mouse MINUS, Sigma DUO92004 ...
-
bioRxiv - Cell Biology 2024Quote: ... Slides were then washed twice with PBS and processed using Duolink Proximity Ligation Assay (PLA) reagents (In-Situ PLA Probe Anti-Mouse MINUS, Sigma DUO92004; In-Situ PLA Probe Anti-Rabbit PLUS, Sigma DUO92002 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Secondary antibodies coupled to horse radish peroxidase (goat anti-rabbit, goat anti-rat, and goat anti-mouse) were purchased from Sigma and used at the recommended dilution.
-
bioRxiv - Cancer Biology 2024Quote: ... The tubes were centrifuged at 6,000 rpm and the supernatants were collected for cytokine analysis by bead-based multiplex assay (MILLIPLEX MAP Mouse High Sensitivity T Cell Panel, Millipore) following the manufacturer protocol27.
-
bioRxiv - Microbiology 2024Quote: ... α-tubulin was detected as a loading control with the mouse monoclonal antibody B-5-1-2 (Sigma, T5168, 1/4000) for 2 hrs at 25°C ...
-
bioRxiv - Neuroscience 2024Quote: ... Then these free-floating tissue sections were incubated with a primary mouse anti-TPH antibody (diluted 1:500, purchased Sigma) for 48 hours at 4 °C ...
-
bioRxiv - Immunology 2024Quote: Mouse cell lines: HC11 cells (CRL-3062, ATCC) were cultured in RPMI-1640 and supplemented with 10% FBS (F2442, Sigma) and 100 U/ml penicillin ...
-
bioRxiv - Bioengineering 2024Quote: ... The primary antibodies used included mouse anti-Integrin alpha 5/CD49e (Snaka51, Catalog Number: MABT201, Sigma-Aldrich, Dilution: 1:500), mouse anti-Integrin alpha V (L230 ...
-
bioRxiv - Cell Biology 2024Quote: ... Fixed cells were labeled with a rabbit anti-KIF5B antibody (Tanaka et al., 1998) and a mouse anti-insulin antibody (Sigma), followed by Alexa-conjugated secondary antibodies (Invitrogen) ...
-
bioRxiv - Bioengineering 2024Quote: ... The samples were then diluted to 1 mg/ml and processed by Eve Technologies using the Mouse High Sensitive 18-Plex Discovery Assay according to the manufacturer’s protocol (Millipore Sigma).
-
bioRxiv - Microbiology 2024Quote: ... Organisms were stained with anti-MOMP (Meridian Biosciences) primary antibody for all the strains and primary mouse anti-Cpf1 (dCas12) (Sigma-Millipore) for knockdown or complementation samples ...
-
bioRxiv - Molecular Biology 2024Quote: ... 0.1% RNAse-free Bovine Serum Albumin for 30 minutes at RT before being incubated with primary antibodies (Thermofisher, mouse anti-tubulin, 1:1000; Sigma mouse monoclonal anti-myc clone 9E10 ...
-
bioRxiv - Immunology 2024Quote: ... and PRL were analyzed in a single run on a Luminex 200 platform using a MILLIPLEX MAP Mouse Pituitary Magnetic Bead Panel (Millipore) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: Mouse organs and ES cells were homogenized mechanically in RIPA lysis buffer supplemented with 2x protease inhibitor cocktail set (Millipore) and loaded with SDS dye ...
-
bioRxiv - Molecular Biology 2024Quote: For aortic vascular permeability measurements, Evans blue dye (20 mg/ml in saline, 2.5 µl/g mouse body weight) (Sigma E2129) was injected retro-orbitally to LPHN2 endothelial cell-specific knockout and their control littermates at 8 weeks old ...
-
bioRxiv - Microbiology 2024Quote: ... 100 µL of overnight cultures grown in M63CA+G was centrifuged and resuspended in 100 µL of PBST (PBS + 0.05% v/v Tween20) containing a 1:100 dilution of mouse monoclonal α-FLAG antibodies (Sigma) and incubated overnight at 4 °C with end-over-end rotation in a microcentrifuge tube ...
-
bioRxiv - Microbiology 2024Quote: ... Blots were washed and then probed with a 1:10,000 dilution of goat α-mouse antibody conjugated to horse radish peroxidase (Sigma). Blots were then incubated with SuperSignal West Pico PLUS Chemiluminescence substrate (ThermoFisher ...
-
bioRxiv - Immunology 2024Quote: ... The cells were washed three times in PBS 1× and incubated for 1 hour at 37°C with 40 μL of mouse anti-DENV complex monoclonal antibody MAB8705 (Merck Millipore) diluted 1:200 in PBS 1× ...
-
bioRxiv - Physiology 2024Quote: Immunoblot analysis was carried out on cell or adipose lysates using mouse or human primary antibodies against MPC1 (Sigma #HPA045119), MPC2 (Cell Signaling Technologies #46141) ...
-
bioRxiv - Plant Biology 2024Quote: Immunoblot analyses were performed using horseradish peroxidase (HRP)-conjugated anti-FLAG M2 mouse monoclonal (Sigma-Aldrich A8592, 1:10,000 dilution) and mouse HRP-anti-MYC monoclonal antibodies (BioLegend #626803 ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: Mouse tissues were diced into pieces and digested in 10 mL RPMI 1640 containing 1mg/mL collagenase-1A (Sigma-Aldrich) make tissues into single-cell suspensions or thorough homogenates by using semi-automated gentleMACS™ Dissociator and incubate for 45 min at 37 °C on an orbital shaker ...
-
bioRxiv - Microbiology 2024Quote: ... the membrane was washed three times with TBS-T before incubating with mouse monoclonal anti-FLAG coupled to HRP (#A8592, Sigma) at a 1:5,000 titer in TBS-T ...
-
bioRxiv - Cancer Biology 2024Quote: ... and a mouse monoclonal anti-ß-actin antibody (1:5,000, A-5441, Sigma-Aldrich and 1:1,000, A1978, Sigma-Aldrich). Afterwards ...