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Citations for Millipore Sigma :
9051 - 9100 of 10000+ citations for Mouse TIG2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... All purified proteins were analyzed by SDS-PAGE followed by Coomassie blue staining as well as by Western blot using a mouse monoclonal antibody against the 6xHis-Tag (Novagen). Protein aliquots were stored at −80⁰C in 50% glycerol (v/v ...
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bioRxiv - Systems Biology 2020Quote: ... At time 0 medium was removed from cells and recombinant mouse tumor necrosis factor alpha (TNF-α, Sigma-Aldrich, #T7539) was added at concentrations 0-100 ng/mL as 5-minute pulse ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitation reactions were performed using anti-FLAG antibody (Monoclonal ANTI-FLAG® M2 antibody produced in mouse, F1804, Sigma-Aldrich). The complex of chromatin antibody was captured with protein G beads (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated overnight at 4°C with the following primary antibodies: mouse anti-tyrosine hydroxylase (TH, 1:1000, MAB318, Millipore), rabbit anti-glial fibrillary acidic protein (GFAP ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were washed multiple times in TBS-T for 1h before adding secondary antibody (goat anti-mouse alkaline phosphatase conjugate; Sigma) as above at 1:2500 dilution ...
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bioRxiv - Microbiology 2021Quote: ... through immunoblotting using anti-His primary antibody raised in mice and monoclonal (goat) anti-mouse secondary HRP-conjugated antibody (Sigma). Further ...
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bioRxiv - Immunology 2020Quote: ... Membrane was then stained with 1 μg/mL of HRP coupled anti-His (monoclonal mouse anti-polyHistidine-Peroxidase, Sigma-Aldrich) in 1% w/V BSA in PBS-T for 1 h at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were transferred to well-plates for exposure to two primary antibodies (RFP antibody 5F8 rat for mCherry, RRID: AB_2336064, Chromotek, 1:1000; and mouse-monoclonal anti-CaMKIIα, RRID: AB_309787, Merck Millipore, 1:300) which were added to 0.1 M PBS-Tx 0.2% containing 5% normal goat serum ...
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bioRxiv - Neuroscience 2020Quote: ... a mixture of primary antibodies in PBS-XD overnight (1 μg/ml rabbit anti-GAD67/65, Frontier; 1:4000 mouse anti-glutamate, ImmunoStar; 1:500 goat anti-ChAT, Millipore), and a mixture of secondary antibodies in PBX-XD for two hours (donkey anti-goat ...
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bioRxiv - Molecular Biology 2020Quote: ... ice cold methanol fixed and 4% dried milk blocked cilia pellets and deciliated cell membranes (excised from Transwell inserts) were labelled for 1 hour at room temperature with a mouse anti-alpha-tubulin antibody (T9026, Sigma) diluted 1:500 in PBST ...
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bioRxiv - Neuroscience 2021Quote: ... and probed with the following antibodies overnight at 4°C: mouse anti-PSD95 (1:2000, Millipore, Cat# MAB1596, RRID: AB_2092365), mouse anti-synaptophysin (1:2000 ...
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bioRxiv - Cancer Biology 2020Quote: ... in 5% BSA-TTBS overnight at 4°C and then HRP-conjugated goat anti-rabbit IgG (Jackson) or goat anti-mouse IgG(Sigma) secondary antibodies for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... on top of 3T3-J2 mouse embryonic fibroblast feeder cells inactivated by mitomycin-C treatment (0.4 μg/ml, Sigma-Aldrich).
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bioRxiv - Cell Biology 2021Quote: ... Bacteria were washed with PBS and opsonized for 30 minutes at room temperature in a 1:1 solution of mouse serum (Sigma) and cell culture media (Gibco) ...
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bioRxiv - Neuroscience 2020Quote: ... and resuspended in culture medium (Neurobasal A, B27, Glutamax, 10% FBS, 100 ng/mL neural growth factor; mouse NGF 7.0s, Millipore Sigma). DRG neurons were then seeded on each corresponding PAA hydrogel at approximately 30,000 cells per 250 mm2 growth area to achieve single cell density ...
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bioRxiv - Biophysics 2020Quote: ... these were washed three times with PBS and incubated for 2h with the anti-mouse IgG (whole molecule)-FITC secondary antibody (Sigma). Myonemes ...
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bioRxiv - Cell Biology 2020Quote: ... and 0.1% sodium azide) and incubated with the following primary antibodies: mouse anti-α-tubulin (DM1α) 1:500 (Millipore Sigma) for one hour at room temperature (RT) ...
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bioRxiv - Neuroscience 2022Quote: ... 1:100 mouse anti-ASCL1, Guillemot lab; 1:800 rat anti-BrdU, Abcam ab6326; 1:800 chicken anti-GFAP, Millipore AB5541 ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies were diluted in high-salt TBS + 2% BSA + 0.1% Triton X-100 at 1:4000 (mouse anti-a-tubulin, Sigma #T6199) and 2 µg/mL (rabbit anti-CENP-A ...
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bioRxiv - Cell Biology 2022Quote: ... Islet architecture was analyzed using isolated mouse islets fixed in 4% PFA overnight followed by a 20 min incubation with methylene-blue solution (Sigma) and OCT embedding ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated for 24-40h at 4°C in mouse anti-GAD67 (1:1000; Sigma Millipore, catalog #MAB5406), rabbit anti-NeuN (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated for 24-40h at 4°C in mouse anti-GAD67 (1:1000; Sigma Millipore, catalog #MAB5406), rabbit anti-NeuN (1:500 ...
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bioRxiv - Immunology 2022Quote: ... the different mouse groups were killed 1 hour after retro-orbital injection of 100 μl of 2% Evans blue (EB) dye (Sigma) and were perfused intracardially with 15 ml of PBS ...
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bioRxiv - Neuroscience 2022Quote: Cytokine levels were measured in spinal cord homogenates by Luminex multiplex bead-based analysis (MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel; Millipore) using the Bio-Plex 200 system (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2022Quote: ... Mouse monoclonal antibodies against MAP2 (M4403, IC: 1:100) and mouse monoclonal antibodies against GAPDH (WB: 1:1000) were from Sigma. Mouse monoclonal antibodies against GAD65 (GAD-6 supernatant ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were centrifuged for 5 min at 20 000 x g and supernatants were immunoprecipitated for 1 h rotating at 4°C with mouse anti-GFP antibody (1 µg antibody per sample, 11814460001 Merck/Sigma) and 20 µl DynabeadsTM Protein G (10004D ...
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bioRxiv - Microbiology 2022Quote: ... Nitrocellulose membrane was washed in TBST for 1h before adding secondary antibody (goat anti-mouse alkaline phosphatase conjugate; catalogue number A3562) (Sigma,) in 5% skim milk TBST at 1:2500 dilution ...
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bioRxiv - Microbiology 2022Quote: ... Plates were washed as before and 100μl/well rabbit anti-mouse IgG1 or IgG2a conjugated to HRP (Sigma-Aldrich, Germany) diluted to 1 ...
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bioRxiv - Microbiology 2022Quote: ... cells were then incubated in a 1:5000 dilution of a horseradish peroxidase (HRP) conjugated goat anti-mouse IgG (Sigma). Titration of RUBV was performed similarly but with a polyclonal anti-RUBV goat IgG at 1:4000 (LifeSpan BioSciences ...
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bioRxiv - Biophysics 2022Quote: ... followed by incubation in LI-COR blocking buffer containing rabbit polyclonal anti-P2X2 (#APR-003, Alomone labs; 1:2000) and mouse anti-Na+/K+-ATPase (05-369; EMD Merck Millipore) at 4 °C overnight ...
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bioRxiv - Neuroscience 2022Quote: ... The sample was then washed twice with 2X SSC and digested by 3 incubations (10 minutes for mouse and 5 min for human) with digestion buffer containing 1% (w/v) sodium-dodecyl-sulfate (SDS, Sigma), 20 mM Tris HCl (Thermo) ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were the resolved in a 12% SDS-PAGE gel and transferred on a 0.2 µm nitrocellulose membrane and then immunobloted using mouse FlagM2 antibody (Sigma-Aldrich) diluted at 1/500.
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bioRxiv - Biophysics 2022Quote: ... Secondary antibodies were goat anti-rabbit and anti-mouse Alexa 488 and Alexa 555 conjugated fluorescence at 1:500 (Millipore). The spinal cord sections were directly coverslipped with Dapi mounting media (Vector ...
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bioRxiv - Biophysics 2022Quote: ... Phagosomes in each chamber were incubated with one of the following mouse monoclonal antibodies against kinesin-1 (MAB1614, EMD Millipore), kinesin-2 (ab24626 ...
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bioRxiv - Cell Biology 2022Quote: ... 2013), rabbit anti-Cep97 (Dobbelaere et al., 2020), rabbit anti-Cnn (Dobbelaere et al., 2008) and mouse anti-Actin (Sigma); all primary antibodies were used at 1:500 dilution in blocking solution (Aydogan et al. ...
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bioRxiv - Developmental Biology 2022Quote: ... Smith’s laboratory) mouse ESC lines were routinely cultured without feeders on gelatin-coated plates (0.2% gelatin, Sigma-Aldrich, cat. G1890). Media were changed every 2 days and cells were passaged when approaching confluency (every 2-3 days) ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were incubated overnight at 4 °C with the primary antibodies: mouse anti-Syntaxin-1 (1:500, Sigma, # S0664), chicken anti-GFP (1:3000 ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were washed three times with Tris-buffered saline and Tween-20 followed by incubation with the peroxidase-conjugated anti-mouse or anti-rabbit secondary antibodies (both from Millipore) for 1 h at room temperature.
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bioRxiv - Biochemistry 2022Quote: ... 0.3% Triton X-100 in PBS) and with primary antibodies overnight at +4 °C (primary Abs: mouse anti-flag –Sigma F1804 ...
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bioRxiv - Cell Biology 2022Quote: ... each strip was incubated with 99 μL PBSBT and 1 μL mouse anti-CNB antibody (Sigma-Aldrich, C0581, 1:100), on the nutator for five hours at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... A mouse anti-myosin heavy chain antibody was used as primary antibody (1:200; Sigma Aldrich, St. Louis, MO, USA) and Alexa 488 donkey anti-mouse IgG antibody as secondary antibody (Life Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... shRNA knockdown vectors for mouse Mdm2 (TRNC0000302276) or human MDM2 (TRCN0000355726, TRCN0000355727, TRCN0000355728, TRCN0000003378 and TRCN0000003380) were from Sigma Aldrich. The pBabe-puro expression vector for CA-AMPK has been described53.
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bioRxiv - Cell Biology 2022Quote: The mouse monoclonal anti-GABAA α1 subunit antibody (clone BD24) (catalog #: MAB339) and mouse monoclonal anti-GABAA β2/β3 antibody (catalog #: 05-474) were obtained from Millipore. The rabbit monoclonal anti-GABAA α1 subunit antibody (catalog # ...
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bioRxiv - Neuroscience 2021Quote: ... and 0.1 % Triton X-100 for 60 min at room temperature and incubated with mouse monoclonal anti-TUBB3 (Sigma; #T8660) (1:1000) ...
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bioRxiv - Neuroscience 2020Quote: Live TTX-treated WT and Fmr1R138Q hippocampal neurons (14-15 DIV) were incubated with a mouse monoclonal anti-N-terminal GluA1 antibody (1:100; Merk-Millipore) for 10 min at RT ...
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bioRxiv - Neuroscience 2020Quote: ... then blocked with 3 % BSA in PBS for 1 h at room temperature and incubated with primary antibodies (mouse anti-GFAP, Sigma, 1:500 ...
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bioRxiv - Immunology 2020Quote: ... fixed antibodies were detected with horseradish peroxidase-conjugated goat anti-mouse IgA (100 µL / well - 1.5 µg/mL; Sigma-Aldrich) for 1h30 at 37°C and the reaction revealed with of 3,3’-5,5’-tetramethylbenzidine peroxidase substrate (100 µL/well - KPL ...
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The cellular basis of protease activated receptor type 2 (PAR2) evoked mechanical and affective painbioRxiv - Neuroscience 2020Quote: ... Slides were placed in a light-protected humidity-controlled tray and incubated in primary antibody (mouse-anti-Neurofilament 200; clone N52; MAB5266; Sigma) at 1:500 in blocking buffer overnight at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Blots were stained with rabbit anti-Actin or rabbit anti-TnI (1:1000; a gift from A. Ferrus) or mouse anti-Tubulin (1:1000, Sigma) and washed with TBS-Triton X (0.1%) ...
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bioRxiv - Microbiology 2020Quote: ... supernatant was collected in clean microcentrifuge tube and incubated 2 h at 4°C with a-flag mouse antibody (Sigma), following 1 h incubation at 4°C with PureProteomeProtein A/G Magnetic Beads Mix (Millipore) ...