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Citations for Millipore Sigma :
9101 - 9150 of 10000+ citations for Mouse TIG2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Membranes were then incubated for 1 h with secondary antibody a-mouse HRP conjugate 1:10000 (Cell Signalling) and chemiluminescent substrate ultra (Millipore) was applied for 5 min before camera detection in Sapphire Biomolecular Imager (Azure Biosystems) ...
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bioRxiv - Developmental Biology 2021Quote: ... 30 μl of Protein G coated Bio-Adembeads (Ademtech) were incubated with either 1 μg of mouse monoclonal anti-FLAG antibody (F3165, Sigma), or goat anti-HA antibody as mock (sc-805 ...
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bioRxiv - Immunology 2020Quote: ... the viral supernatant was harvested and used to transduce 25,000 mouse primary keratinocytes seeded in 48-well plates in the presence of 8 µg/ml polybrene (Sigma Aldrich). The next day ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were incubated overnight at 4°C with the following primary antibodies: mouse anti-RELN G10 (MAB5364, Millipore 1:2000) or 142 (for human RELN ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclei were isolated from a single mouse hippocampus in 2 ml of pre-chilled EZ PREP lysis buffer (NUC-101, Sigma) using a glass Dounce tissue grinder (D8938 ...
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bioRxiv - Neuroscience 2021Quote: ... and APC rat anti-mouse 4D4 (marking resident microglia; 1:1000) in blocking buffer containing 0.2% bovine serum albumin (BSA, Sigma-Aldrich) in HBSS ...
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bioRxiv - Neuroscience 2021Quote: ... Isolated nuclei were labelled in suspension in 1 ml PBS buffer with 1:500 anti-NeuN antibody (Millipore, MAB377, mouse) and 1:250 anti-Sox10 antibody (R&D ...
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bioRxiv - Neuroscience 2021Quote: ... then the Duolink In Situ PLA Probe Anti-Rabbit PLUS and Duolink In Situ PLA Probe Anti-Mouse MINUS PLA probes (Sigma) diluted in Duolink Antibody Diluent were applied ...
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bioRxiv - Neuroscience 2021Quote: ... Isolated nuclei were labelled in suspension in 1 ml PBS buffer with 1:500 anti-NeuN antibody (Millipore, MAB377, mouse) and 1:250 anti-Sox10 antibody (R&D ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were incubated with OXT and AVP primary antibodies (mouse anti-oxytoxin, Gainer Lab, NIH, PS3845,60 and rabbit anti-vasopressin, Millipore PC234L) diluted in 0.1 % saponin (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were detected with horseradish peroxidase conjugated anti-rabbit and anti-mouse antibodies and visualized by enhanced chemiluminescence detection (Luminata Forte Western HRP substrate, Merck Millipore). Digital images were acquired on a ChemiDoc XRS System (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... The membranes were blocked with 5% bovine serum albumin (BSA) and then probed with the following primary antibodies - mouse anti-GluN2A (1:1000, Millipore), rabbit anti-GluN2B (1:1000 ...
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bioRxiv - Microbiology 2021Quote: Western blot analyses of 3xFLAG-tagged proteins or IgaA were carried out as described previously (Pontel and Soncini, 2009; Pérez Audero et al., 2010) with mouse anti-FLAG monoclonal (Sigma-Aldrich) or rabbit polyclonal anti-IgaA antibodies ...
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bioRxiv - Biochemistry 2020Quote: ... Bound scFv were detected using murine mAb 9E10 which recognizes the C-terminal c-myc tag (1:50 diluted in 2% MPBST) and a goat anti-mouse serum conjugated with horseradish peroxidase (HRP) (A0168, Sigma) (1:42000 dilution in 2% MPBST) ...
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bioRxiv - Bioengineering 2020Quote: ... or mouse monoclonal β-actin antibody (1:1000 dilution; catalog no. catalog no. MAB 1501; Merck Millipore, Burlington, MA, USA) at room temperature for 1 hour ...
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bioRxiv - Genetics 2022Quote: ... The slices then incubated with primary antibodies with denoted dilutions overnight at 4°C (anti-Iba1 goat polyclonal, Abcam ab5076, 1:200; anti-HNA mouse monoclonal, Millipore MAB1281 ...
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bioRxiv - Neuroscience 2022Quote: All samples were immunostained for fluorescence activated cell sorting (FACS) with mouse anti-NeuN conjugated to PE (EMD Millipore, FCMAB317PE) at a dilution of 1:500 with incubation for 30 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... and spike protein-bound antibodies were detected using an Anti-Mouse IgG (Fc Specific)-Alkaline Phosphatase antibody (Sigma-Aldrich, #A2429) at a 1:4000 dilution in blocking buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were then pre-cleared using 25 μL of Protein A agarose beads (Pierce) and then incubated with 25 μL of mouse anti-V5 agarose beads (Sigma) and washed with RIPA buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... Horseradish peroxidase conjugated secondary antibodies were added to the membranes (anti-rabbit, Invitrogen; 31460 and anti-mouse EMD Millipore; AP308P). Membranes were developed with ECL Western Blotting Substrate (Thermo Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... anti-mouse IgG (H+L) HRP conjugated (Chemicon International AP308P) and Anti-Rabbit IgG (whole molecule)–Peroxidase (Sigma Aldrich A9169). Chemiluminescence detection was done with SuperSignal West Pico or Femto (Thermo Fischer Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... R26-CreERT2/R26-Confetti mice received intraperitoneal injections of tamoxifen (TAM; 4mg/25 grams mouse weight, Sigma, St. Louis, MO) dissolved in 10% ethanol/corn oil (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... After blocking in 5% BSA, membranes were incubated with Anti-FASN rabbit antibody (#3189, CST) antibody or mouse anti-tubulin antibody (Sigma). Protein was next incubated with HRP-conjugated secondary antibodies and was detected using chemiluminescence.
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bioRxiv - Plant Biology 2022Quote: ... A 1–month–old ICR mouse (SLAC laboratory animal) was injected with 50 μg GST–CAR9 protein emulsified with Complete Freund’s adjuvant (F5881, Sigma–Aldrich). Two weeks later ...
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bioRxiv - Neuroscience 2019Quote: ... and or Alexa Flour 488 anti-mouse IgG (1:800, A11001, Life) at room temperature for 1h and mounted with DAPI (F6057, Sigma). The sections were observed using fluorescence microscope (Olympus BX35 ...
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bioRxiv - Biochemistry 2019Quote: The custom designed CompoZr® Zinc Finger Nuclease Plasmid targeted to exon 3 of the mouse ECH1 gene on chromosome 7 was obtained from Sigma. In-vitro transcription of the ZFN mRNA was performed following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... containing 4% PFA (EM Sciences) and 0.1% Triton X-100 and stained with a mouse antibody to α-tubulin (DM1α; SIGMA) and a secondary donkey DyLight 594-conjugated F(ab’)2 mouse IgGs (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Developmental Biology 2019Quote: ... The serum-containing mESCs medium was composed of high glucose DMEM (HyClone) supplemented with 1000 U/mL recombinant mouse leukaemia inhibitory factor (LIF) (Millipore), 100 μM non-essential amino acids (NEAA ...
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bioRxiv - Plant Biology 2020Quote: ... Sections were blocked with 5 % bovine serum albumin (BSA) in PBS before their incubation with an anti-HA mouse monoclonal antibody (Sigma) for 2 hours at room temperature ...
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bioRxiv - Genetics 2019Quote: ... Monoubiquitin forms of 8.5 kDa were detected using a mouse monoclonal anti-ubiquitin primary antibody (Sigma, U0508, 1:3000 dilution) and a peroxidase-conjugated AffiniPure goat anti-mouse (Jackson Immuno Research ...
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bioRxiv - Microbiology 2020Quote: ... the Duolink in situ PLA probe anti-mouse MINUS and the in situ detection reagent Green (Sigma-Aldrich, Milan, Italy) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Each slide was then incubated overnight at 4° with a 1:100 dilution of mouse monoclonal anti-a-tubulin primary antibody (clone DM1A, Sigma) in blocking buffer ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes with transferred proteins were blocked/permeabilized for 1 hour in 5% BSA in TBS and incubated with different antibodies (mouse anti-pMLKL, Millipore, rabbit anti-MLKL ...
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bioRxiv - Plant Biology 2019Quote: ... Sections were blocked with 5 % bovine serum albumin (BSA) in PBS and then incubated with 1:50 anti-HA mouse monoclonal antibody (Sigma) in PBS at room temperature for 2 h ...
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bioRxiv - Plant Biology 2019Quote: ... Primary antibody was washed three times with PBS for 15 min and subsequently incubated with 1:40 Alexa 594 - conjugated anti-mouse rabbit monoclonal antibody (Sigma) in PBS at room temperature for 1 h ...
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bioRxiv - Genomics 2019Quote: ... we blocked coverslips with 5% BSA in PBT for 1 hour at room temperature and then incubated coverslips with properly diluted primary antibody (mouse M2 monoclonal ANTI FLAG, 1:800, F1804, Sigma; rabbit polyclonal Tom20 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... membranes were blocked for 1 h at RT in 5% skimmed milk powder and 0.2% Tween in PBS before incubating overnight at 4◦C with the primary antibodies mouse anti-TH (1:1000, Millipore) and rabbit anti-GAPDH (1:1000 ...
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bioRxiv - Cell Biology 2019Quote: ... Coverslips were blocked for 30 min in 4% Bovine Serum Albumin then incubated overnight with a mouse anti-puromycin monoclonal antibody (Millipore) (1:1000 in 4% BSA) ...
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bioRxiv - Cell Biology 2019Quote: ... and seeded in a concentration of 1.6 × 105 cells/cm2 (passage 39) on mouse laminin (5 µg/cm2, Sigma-Aldrich) coated 0.4 μm pore polyester membrane inserts (Corning) ...
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bioRxiv - Developmental Biology 2020Quote: ... The trophectoderm was removed by immunosurgery and “epiblasts” were grown in gelatinised dishes in the presence of NDiff + 2i and ESGRO (recombinant mouse LIF Protein, Millipore) to establish ESC lines.
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bioRxiv - Synthetic Biology 2020Quote: ... and incubated for 1 h at room temperature or overnight at 4°C in primary antibody (Mouse-anti-FLAG M2 [Sigma #F1804 ...
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bioRxiv - Genetics 2020Quote: ... Cells were incubated overnight at room temperature in blocking buffer containing primary antibody (mouse anti-UBE3A (Sigma SAB1404508 clone 3E5)) at 1:250) ...
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bioRxiv - Cell Biology 2019Quote: ... mES cells were cultured either on a monolayer of gamma irradiated mouse embryonic fibroblasts (IRR-MEFs; StemGent) or gelatin coated dishes (Sigma). Fused monolayers of NIH/3T3 cells and bFFs were cultured in fibroblast medium ...
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bioRxiv - Pathology 2020Quote: Mouse primary hepatocytes were isolated by gradient density centrifugation of 30% Percoll solution after liver perfusion using Collagenase II (Sigma). Primary hepatocytes were cultured in William’s E Medium (Thermo Fisher Scientific ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... The rehydrated slides were blocked with 2% horse-serum followed by incubation with primary antibodies (Mouse anti-S-glycoprotein antibody, 1:200 dilution, Millipore, Cat # MAB5676 ...
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bioRxiv - Pathology 2020Quote: ... IP was performed by incubation of the resulting lysates overnight at 4 °C with mouse monoclonal anti-Flag antibody (Cat.# F3165, Sigma) or with normal mouse IgG (Cat.#A7028 ...
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bioRxiv - Immunology 2020Quote: ... the membranes were incubated with the appropriate horseradish-peroxidase conjugated secondary Ab (1:5000 in TBST + 2% BSA; 2 h at room temperature; anti-mouse A4416 and anti-rabbit A0545, Sigma). The immunoreactivity was detected by ECL reagents (Amersham) ...
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bioRxiv - Developmental Biology 2019Quote: Protein lysates of tissue isolated from the tail end of mouse embryos at E10.5 were investigated with Rabbit anti-SNX14 (Sigma, HPA017639) as previously reported (9).
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bioRxiv - Developmental Biology 2020Quote: ... mouse monoclonal anti-Tau-1 (05-838, 1:1000), mouse monoclonal anti-MAP2 (05-346, 1:500), rat monoclonal anti-tubulin (MAB1864, 1:500) from Millipore; chicken polyclonal anti-GFP (ab13970 ...
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bioRxiv - Developmental Biology 2021Quote: ... were grown on 0.1% gelatin-coated flasks in 8% CO2 at 37C and cultured in mouse ES media serum+2i+LIF: DMEM (Sigma) without phenol-red ...