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Citations for Millipore Sigma :
9301 - 9350 of 10000+ citations for Mouse TIG2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... France) and secondary (anti-mouse IgG peroxidase-conjugated, 1:1000 in PBS + 1% BSA, Sigma Aldrich, St. Louis, MO, USA) antibodies were added at RT for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates and immunoprecipitates were analyzed on Western blots using mouse monoclonal anti-MYC antibody (1:800; Covance) (for exogenous 14-3-3ε) or anti-FLAG antibody (1:800; Sigma) (for exogenous Cdc25B ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 20 ng/mL mouse GM-CSF (Miltenyi #130-095-739) and 0.5 μM β-estradiol (Sigma-Aldrich #E2758)] and transduced with 1:5 (vol:vol ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies coupled to horseradish peroxidase (HRP) were anti-rat-HRP at 1:10000 and anti-mouse-HRP at 1:10000 (Sigma).
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bioRxiv - Bioengineering 2023Quote: ... The supernatant solution was removed and the cells were treated with a secondary antibody (the FITC-conjugated anti-mouse IgG (Sigma)) for one hour at 37°C in the darkness ...
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bioRxiv - Neuroscience 2023Quote: ... 0.1% Tween in TBS at room temperature and incubated overnight at 4 °C with primary antibodies against digoxigenin (mouse monoclonal 1:500, Sigma) and β-actin (rabbit polyclonal 1:200 ...
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bioRxiv - Neuroscience 2023Quote: Western blotting of adult head lysates (three heads per sample with one head loaded per lane) was performed using standard laboratory procedures with mouse anti-Tubulin (T5168; Sigma) at 1:10000 (UAS rescue experiments ...
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bioRxiv - Molecular Biology 2023Quote: ... Full-length FN and 45 kDa-FN GBD were detected with monoclonal mouse anti-FN antibody with epitope within GBD (MAB1892, Sigma), clone IST-10 (1:1000 in 2% BSA in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... S4 a similar protocol was used but the primary antibody mix consisted of mouse anti-GFP 1:1000 (Sigma G6539), rabbit anti-RFP 1:5000 (Rockland 600-401-379) ...
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bioRxiv - Cell Biology 2023Quote: ... The following antibod-ies were used for western blots of human cell extracts: Mouse anti-FLAG (Sigma F1804, 1:1000); Mouse anti-GAPDH (Proteintech 60004-1-Ig ...
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bioRxiv - Molecular Biology 2023Quote: ... PLA reactions were subsequently performed using Duolink PLA probes for goat-mouse and goat-rabbit and Duolink In Situ Detection Reagents Red (Sigma) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... Samples were then transferred to a PVDF membrane and analyzed by Western blot using a mouse-anti-myc primary antibody (Sigma) and HRP-coupled goat-anti-mouse secondary antibody (Jackson ImmunoResearch) ...
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bioRxiv - Cell Biology 2023Quote: The following antibodies were used in this study: monoclonal mouse antibody to BIN1 (clone C99D against exon 17) from Millipore, polyclonal rabbit antibody to IRSp53 (BAIAP2 ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated with secondary-HRP conjugated antibodies (1:10.000; Anti Rabbit IgG HRPO, Millipore BI2407; Anti Mouse IgG HRPO, Millipore BI2413C) for 1h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with the primary antibody OC (1:500 for mouse, 1:200 for human; polyclonal, AB2286, Millipore) and respective secondary antibody (1:400 ...
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bioRxiv - Cell Biology 2023Quote: ... Rabbit polyclonal anti-pS226 GLUT1 antibody (ABN991) and mouse anti-phosphotyrosine monoclonal antibody cocktail 4G10 Platinum (05–1050) were purchased from Millipore. Recombinant polyclonal anti-ALDOA antibody cocktail (711764 ...
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bioRxiv - Microbiology 2023Quote: ... The secondary antibodies used for IB were goat anti-rabbit (Cat # AP307P) and goat anti-mouse (Cat # AP308P) IgG HRP-conjugated antibodies (Merck Millipore).
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bioRxiv - Biochemistry 2023Quote: ... The proteins were resolved in SDS-PAGE gels and transferred to nitrocellulose membranes that were incubated overnight at 4 °C with one of the following antibodies: mouse monoclonal anti-hemagglutinin (1:2000, Sigma); rabbit anti-P-TrkB-Tyr674/675 (1:1000 ...
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bioRxiv - Biochemistry 2023Quote: Mouse monoclonal anti-FLAG M2 (#F3165) and anti-CD95 mAb CH-11 (#05-201) was from sigma-Aldrich (Sigma Aldrich Chimie ...
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bioRxiv - Biochemistry 2023Quote: ... the plates were incubated with 100 μL/well horseradish peroxidase-conjugated anti-mouse immunoglobulin second antibody (Sigma, St. Louis, MO) in Buffer D of pCa 9 or 4 at room temperature for 1 h ...
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bioRxiv - Cancer Biology 2022Quote: ... were cultured in the presence of recombinant mouse IL-4 (25 ng/mL; #404-ML, R&D) and LPS (20 μg/mL; #L2880, Sigma) over night to induce B cell activation and terminal differentiation ...
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bioRxiv - Cell Biology 2023Quote: ... mESCs were grown at 37°C and 5% CO2 on Mitomycin-C inactivated feeder cells (DR4-mouse embryonic fibroblast) plated on 0.1% gelatin (Sigma-Aldrich) in serum/leukemia inhibitory factor (LIF ...
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bioRxiv - Cancer Biology 2023Quote: The mouse E0771 (derived from a spontaneous triple negative mammary tumor that arose in a C57BL/6 mouse) and the human K562 (originally generated from a female leukemia patient) cell lines were cultured in RPMI-1640 (Sigma) supplemented with 10% FCS (GIBCO) ...
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bioRxiv - Genetics 2022Quote: ... for CLK and BMAL1 expression then the secondary antibody of horseradish peroxidase-conjugated either anti-mouse or anti-rabbit IgG antibody (Sigma). Chemiluminescence detection was performed by using ECL Western Blotting detection Reagent (GE Healthcare) ...
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bioRxiv - Developmental Biology 2023Quote: ... The samples were incubated overnight in a humid chamber at 4°C with the following primary antibodies: mouse anti-pan polyglycylated tubulin (1:1000, Axo-49; Sigma) and anti-cleaved caspase 3 (1:500 ...
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bioRxiv - Immunology 2023Quote: ... in FACS-blocking buffer (mixture of 0.66% human/rabbit/mouse serum, Sigma-Aldrich, and 1% Bovine Serum Albumin, Sigma-Aldrich) for 40 minutes at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... After a further 1 hour incubation at 37°C plates were washed and 50 μl 1:2000 diluted cross-adsorbed anti-mouse HRP (Sigma) was added to each well and incubated for 1 hour at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... blots were incubated overnight at 4°C at the following dilutions: Mouse α-FLAG (1:1000, M2 clone, Sigma #F3165), Mouse α-V5 (1:1000 ...
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bioRxiv - Microbiology 2023Quote: Protein extract from leaf tissue was incubated for 2h at 4°C with an anti-flag mouse antibody at 1:4,000 dilution (Sigma, F1804), followed by a 1h-incubation at 4°C with PureProteomeProtein A/G Magnetic Beads Mix (Millipore) ...
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 lung titers were quantified by homogenizing mouse lungs in 1ml phosphate-buffered saline (PBS; Quality Biological Inc.) using 1.0mm glass beads (Sigma Aldrich) and a Beadruptor (Omni International Inc) ...
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bioRxiv - Neuroscience 2023Quote: ... After treatment with the secondary antibody (anti-rabbit HRP-conjugated, 1:1000 or anti-mouse HRP-conjugated, 1:1000, Sigma), membranes were incubated in chemoluminescent (SuperSignal West Femto ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... Immunofluorescence in human cells was conducted as previously described using antibodies against tubulin (1:1000 anti-beta tubulin, mouse, T7816, Sigma OR 1:2000/1:1000 anti-alpha tubulin ...
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bioRxiv - Neuroscience 2023Quote: ... and primary antibodies (SDC1 Rabbit Thermo Fisher Scientific, MA5-32600, 1:500 dilution; GFAP mouse Sigma G3893, 1:500 dilution). After extensive PBS washings ...
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bioRxiv - Neuroscience 2023Quote: ... then with the primary antibodies in the same solution overnight at 4°C (SDC1, Rabbit Thermofisher MA5-32600, 1:500 dilution; GFAP, mouse Sigma G3893 ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated for 2 hours with secondary antibody (Goat anti-mouse IgG-HRP or Goat anti-rabbit IgG-HRP, Sigma) diluted in blocking buffer ...
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bioRxiv - Immunology 2023Quote: ... Mouse monoclonal anti-β-actin (A5441, clone AC-15) and rabbit polyclonal anti-Calnexin (#C4731) were obtained from Sigma-Aldrich. Mouse monoclonal anti-V5 (BLD-680605 ...
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bioRxiv - Cancer Biology 2023Quote: ... mouse NAFs were transduced using lentiviruses containing shRNA constructs against Postn with 10 mg/ml polybrene (Sigma, TR-1003-G). shPostn1 (F primer ...
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bioRxiv - Pathology 2023Quote: ... total RNA was extracted from the aortic vessels isolated from each mouse using Trizol Reagent (Sigma Aldrich, St. Louis, MO), per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Incubation with primary antibodies was performed overnight at 4°C at the following concentrations: FLAG (mouse, 1:1000; Sigma F1804), HA (mouse ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 hr at room temperature (RT) and incubated with mouse monoclonal anti-aggrecan neoepitope antibody (10 µg/ml; clone AF-28, MAB19310-C, Millipore) and rabbit polyclonal anti-α-tubulin antibody (2 µg/ml ...
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bioRxiv - Microbiology 2023Quote: ... or anti-Ace-2 antibody (ab15348) followed by the respective secondary antibodies (horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG; Sigma). Mouse-monoclonal anti-β-actin-peroxidase antibody (A3854 ...
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bioRxiv - Immunology 2022Quote: ... The plates were then washed and incubated with 100 μl per well of samples prepared as described above and with mouse anti-human αvβ6 (Millipore, MAB2077Z) from 312.5 ng/ml to 1.22 ng/ml to produce the standard curve for 60 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... and probed for EGFP with rabbit anti-EGFP diluted 1:5000 (kind gift from C. Ellermier) or for FLAG with mouse anti-FLAG M2 diluted 1:500 (Milipore-Sigma).
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bioRxiv - Plant Biology 2023Quote: ... Membranes were washed and further incubated with secondary antibody (goat anti-mouse AP, 1:15000; Sigma Aldrich, St. Louis, MO). The membrane was washed with TBS-Tween 20 (TBS-T ...
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bioRxiv - Cell Biology 2023Quote: ... mouse monoclonal anti-PP2A C-subunit clone 1D6 conjugated with Alexa Fluor 488 (Sigma-Aldrich, 05-421-AF488, 1:50). Anti-human Cy3 (Jackson ImmunoResearch ...
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bioRxiv - Genomics 2023Quote: ... Expression of the Dam-POI in the mouse ESC lines was suppressed by addition of 0.5 mM indole-3-acetic acid (IAA; Sigma, I5148).
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bioRxiv - Systems Biology 2023Quote: ... All membranes were blocked in a 5% milk solution and then incubated for 12h at 4°C with either the mouse anti-phosphotyrosine 4G10 (05321, Millipore) or the rabbit anti-GFP (Thermo A11122 ...
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bioRxiv - Plant Biology 2023Quote: ... as primary antibodies and Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L) (115-035-003, Jackson) or Peroxidase Conjugated Goat Anti-Rabbit IgG (H+L) (AP132P, Sigma) as secondary antibodies ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated for 48 h in the primary antibodies: Monoclonal mouse anti-parvalbumin (1:5000; P3088; Sigma-Aldrich) and Wisteria floribunda lectin (1:500 ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were then washed briefly and incubated at 37°C for 30 min in 20% anti-mouse serum antibody (Sigma) or 20% anti-human serum antibody (Sigma) ...