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Citations for Millipore Sigma :
9151 - 9200 of 10000+ citations for Mouse TIG2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The secondary antibodies used in this study were as follows: anti-mouse IgG whole molecule-Peroxidase (1:80,000; catalog no. A9044, Sigma-Aldrich) and anti-rabbit IgG whole molecule-Peroxidase (1:80,000 ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were further incubated for two nights at 4°C with mouse ACTA2-FITC antibodies (1:200; Sigma, F3777) in blocking buffer containing 1-5% bovine serum albumin in 0.25 – 0.5% Triton-X 100 in 1× PBS ...
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bioRxiv - Neuroscience 2021Quote: ... mouse monoclonal anti-synaptophysin (1:500; SMC-178D, StressMarq Bio.) and rabbit monoclonal anti-GluR1 (1:500; 04-855, Millipore). Secondary antibodies were Alexa Fluor 488-donkey anti-mouse IgG (1:300 ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were incubated free-floating overnight at RT with the mouse G10 anti-reelin primary antibody (1:3000, MAB5406; Millipore) diluted in the blocking solution ...
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bioRxiv - Neuroscience 2021Quote: Immunohistochemical staining was performed on free-floating brain sections using the following primary antibodies: mouse anti-rat TH (1:4000, MAB318, Merck Millipore), rabbit anti-Girk2 (1:500 ...
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bioRxiv - Physiology 2020Quote: ... the membrane was incubated with rabbit anti-Urn8R (1:1000) and mouse anti-tubulin (1:2500; Sigma-Alrich, MO, USA) in blocking buffer supplemented with 0.2% Tween 20 (w/v) ...
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bioRxiv - Developmental Biology 2020Quote: ... Sections were then blocked with 5% normal donkey serum in 1X PBS for one hour before overnight incubation in primary antibodies (Foxf1, goat, R&D, 1:300; Sox9, mouse, Invitrogen, 1:200; Sox9, rabbit, Millipore, 1:200 ...
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bioRxiv - Immunology 2021Quote: ... blocked in 5% BSA for 30 min and incubated ON with mouse anti-GAD67 monoclonal antibody (1:6000, MAB5406, Merck Millipore) or 1 h with rabbit polyclonal anti-GAPDH antibody (1:3000 ...
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bioRxiv - Molecular Biology 2021Quote: ... Zygotes were harvested from oviducts at 28 h after hCG injection and then cultured in KSOM Mouse Embryo Media (Millipore) at 37°C with 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... Blots were incubated with goat anti-mouse horse radish peroxidase (HRP)-conjugated secondary antibody and the bands developed with Luminata Forte Western HRP Substrate (Merck Millipore).
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bioRxiv - Microbiology 2021Quote: ... Sera were incubated for one hour at room temperature followed by three washes with PBST before incubating with an anti-mouse IgG secondary antibody conjugated to alkaline phosphatase (Sigma) used as a 1:5000 dilution for one hour ...
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bioRxiv - Synthetic Biology 2020Quote: ... Expression of GAPDH was used as a loading control using a mouse anti-GAPDH antibody (1:10000 dilution, MAB374, Sigma) and IRDye 680CW goat anti-mouse IgG secondary antibody (1:5000 dilution ...
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bioRxiv - Pathology 2021Quote: ... postmortem kidney was fixed in 20% formalin for 2 weeks and processed for free-?oating preparation as done for mouse tissue with one exception that anti-human CD31 antibody (CBL468, 1:20 dilution, Millipore) was used.
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bioRxiv - Systems Biology 2020Quote: ... Immunoprecipitation was carried out by incubating the lysate with the monoclonal anti-FLAG M2-mouse primary antibody (Sigma Aldrich, F1804) and protein G Dynabead (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... The following primary antibodies were used for the Western blotting: mouse antibodies against Flag (Sigma-Aldrich, St. Louis, MO, USA) and mouse antibody against β-actin (Proteintech Group ...
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bioRxiv - Cell Biology 2020Quote: ... and the mouse monoclonal and rabbit polyclonal anti-phosphorylated histone H3 (Ser10) antibodies (ref. 06570, 1:1000) were obtained from Millipore. The anti-GFP mouse monoclonal antibody mix (ref ...
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bioRxiv - Synthetic Biology 2020Quote: ... The membrane was then washed several times with Tris-Buffered Saline with 0.1% Tween 20 (TBST) before a 30 in incubation with monoclonal Mouse Anti-FLAG M2-Peroxidase (HRP) antibodies (Sigma-Aldrich) diluted 1:5000 in TBST ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for 24 hours at 4 °C in mouse anti-GAD67 (1:1000; Millipore Sigma, cat# MAB5406), rabbit anti-NeuN (1:500 ...
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bioRxiv - Plant Biology 2021Quote: ... GSTU5 was detected by the anti-GST primary antibody (Novex, 1:500) and anti-mouse secondary antibody (Sigma, 1:5000).
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bioRxiv - Immunology 2020Quote: ... The pLKO vectors either encoded two specific shRNAs against mouse Akt1 (Cat# TRCN0000304683) or a non-silencing control shRNA sequence (Cat# SHC002) (all from Sigma). Akt1 shRNA and control non-silencing shRNA viruses were intratracheally instilled in mice in a volume of 50 µl ...
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bioRxiv - Immunology 2019Quote: ... 0.1% [w/v] saponin in PBS) for 25 min at RT and then stained with mouse anti-flavivirus antibody (clone D1-4G2-4-15, Millipore) diluted 1:500 in FACS wash buffer for 90 min at RT ...
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bioRxiv - Plant Biology 2019Quote: ... the blot was washed with 1×TBS-T solution (4×5min) and incubated with anti-Mouse IgG-HRP secondary antibody solution (Sigma, 1:10,000 dilution in 1×TBS-T with 0.2% Blocking Reagent [Roche] ...
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bioRxiv - Developmental Biology 2020Quote: HRP-conjugated secondary antibodies for Western blotting: goat anti-mouse IgG (Cat# A 4416, Sigma-Aldrich, St. Louis, MO, USA) and goat anti-rabbit IgG (Cat# GtxRb-003-EHRPX ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were resolved in SDS-PAGE gels and transferred to nitrocellulose membranes that were incubated overnight at 4 °C with one of the following antibodies: mouse monoclonal anti-HA (1:2000, Sigma); rabbit polyclonal MBP-probe (1:1000 ...
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bioRxiv - Microbiology 2019Quote: ... Plates were washed twice with PBS and incubated in same conditions with horseradish peroxidase (HRP)-conjugated goat anti-mouse secondary antibody (Sigma), diluted 1:1000 in blocking buffer ...
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bioRxiv - Biochemistry 2020Quote: ... The Flag-tagged receptor was detected using mouse anti-Flag M2 primary antibody (1:10,000 dilution; Sigma Aldrich, catalog #F3165) and IRDye® 680RD-conjugated donkey anti-mouse IgG secondary antibody (1:20,000 dilution ...
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bioRxiv - Microbiology 2019Quote: ... The cells were then treated with 5% BSA blocking solution for 1 h and the incubated with mouse monoclonal anti-HA (Sigma) antibody and rabbit anti-Flag antibody (Sigma ...
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bioRxiv - Immunology 2019Quote: ... Mouse neutrophil was isolated from the peritoneal cavity 3 hours after injection of 1 ml thioglycollate medium (Sigma-Aldrich, MO). Isolated neutrophils were incubated at 37C for 6 hours and then treated with 10 ng/ml ...
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bioRxiv - Physiology 2020Quote: ... and probed overnight (4 °C) with one of the following primary antibodies: Anti-TSG-6 mouse monoclonal (1 μg/ml; Millipore); rat monoclonal TNFα (1 μg/ml ...
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bioRxiv - Neuroscience 2019Quote: ... and grown in standard human embryonic stem (ES) cells media supplemented with 6ng/ml basic Fibroblast Growth Factor (bFGF) on inactivated mouse embryonic fibroblast (MEFs) (Sigma) as feeder cells ...
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bioRxiv - Bioengineering 2019Quote: For Flag-tag detection membranes were incubated for two hours with the monoclonal anti-Flag M2 antibody from mouse (F3165, Sigma) in a 1:5000 dilution (in TBS-T ...
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bioRxiv - Physiology 2019Quote: Mouse islets were isolated as previously described61 using Type XI collagenase (1000 units/mL; #C7657, Sigma-Aldrich, St. Louis, MO). Following digestion and filtration ...
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Significant control of Zika infection in macaques depends on the elapsing time after dengue exposurebioRxiv - Immunology 2019Quote: ... Plated were washed twice and incubated 1hr/37C/5%CO2/rocking with horseradish peroxidase (HRP)-conjugated goat anti-mouse antibody (Sigma), diluted 1:1,000 in blocking buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were incubated with the following primary antibodies for 1 h at room temperature in Abdil: mouse anti-human lamin A/C (1:200; Millipore), rabbit anti-lamin A/C (1:200 ...
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bioRxiv - Cancer Biology 2021Quote: ... All single-cell samples were distributed at 0.5×106 cells/mL for live staining with monoclonal mouse anti-human FOLR1 IgG1 (LS Bio) in DPBS with 2% v/v FBS (Sigma). Secondary staining for target was performed using QIFIKIT® (BIOCYTEX ...
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bioRxiv - Cell Biology 2021Quote: ... and rabbit monoclonal anti-phospho-PKA substrate (clone 100G7E, CST, 1:1,000) and mouse monoclonal anti-phosphotyrosine (1 μg/ml; clone 4G10, Sigma Aldrich) and acetylated tubulin (1:20,000 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 48 h at 4 βC with primary antibodies (rat anti-BrdU, 1:500, OBT Serotec; mouse anti-NeuN, 1:200, MAB377, Millipore; rabbit anti-S100β ...
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bioRxiv - Developmental Biology 2021Quote: ... Antibodies used were chicken anti-GFP (1:10,000, Aves GFP-1010) and mouse anti-β-tubulin (1:5000, Sigma T4026).
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bioRxiv - Evolutionary Biology 2020Quote: ... the membrane was incubated overnight at 4 °C with the first antibody (rabbit anti-Tc-Zen1 and anti-Tc-Zen2 as for immunohistochemistry, 1:1,000; mouse anti-Tubulin [Sigma-Aldrich #T7451 ...
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bioRxiv - Biochemistry 2021Quote: ... the membranes were probed with a 1:10,000 dilution of alkaline-phosphatase-conjugated goat anti-mouse IgG secondary antibody (Sigma-Aldrich) in PBST ...
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bioRxiv - Biochemistry 2021Quote: Antibodies used for Western Blot include mouse monoclonal anti β-actin (clone AC-15, Sigma, St. Louis, MO, United States) at 0.13 μg/ml ...
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bioRxiv - Biochemistry 2021Quote: ... samples were boiled and resolved by SDS-PAGE followed by western blotting with mouse FLAG (1:2,500; F3165, Sigma-Aldrich), rabbit MYC (1:2,000 ...
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bioRxiv - Biochemistry 2021Quote: ... Inputs and eluates were separated by SDS-PAGE and transferred to nitrocellulose for Western blotting with mouse anti-FLAG (1:2,500; Sigma F3165) and rabbit anti-calnexin (1:3,000 ADI-SPA-865 ...
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bioRxiv - Immunology 2021Quote: ... Cytokines and chemokines in the homogenates were quantified using Luminex technology with a MILLIPLEX Map Mouse Cytokine/Chemokine Magnetic Bead Panel 25-plex Immunology Multiplex Assay (Cat. No. MCYTOMAG-70K-PMX, Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... standard Western blot was performed using anti-SST and anti-α-Tubulin (1:8,000, B-5-1-2, mouse monoclonal, Sigma).
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bioRxiv - Pathology 2021Quote: ... Splenocytes were isolated from wild-type mouse spleens and cultured overnight with 100ng/ml LPS and 5ug/ml PHA (Sigma). The cells were then labeled with cell trace violet (CTV ...
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bioRxiv - Cell Biology 2020Quote: ... slices from mouse brains 10 months (300 days) of age or older were stained with Thioflavine S (Thio-S, Sigma). Slices were washed with 0.1 M PB three times ...
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bioRxiv - Cell Biology 2021Quote: ... a mouse monoclonal antibody against a highly conserved epitope of chicken gizzard actin (clone C4) from EMD Millipore (Burlington, MA), and a mouse monoclonal antibody against the Ser19 phosphorylated form of the myosin II regulatory light chain (P-MyoRLC ...
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bioRxiv - Cell Biology 2020Quote: ... electroporated parasites were mixed immediately with 100 μl of reticulocyte-rich blood from a naïve mouse treated with phenylhydrazine (6 mg/ml) (Sigma-Aldrich), incubated at 37°C for 20 min and then injected intraperitoneally into another mouse ...
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bioRxiv - Cell Biology 2020Quote: ... were washed with 100 μl worm protein lysis buffer and incubated with monoclonal M2 mouse anti-Flag antibody (Sigma, F3165) or monoclonal rabbit anti-c-Myc antibody (Sigma ...