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Citations for Millipore Sigma :
8951 - 9000 of 10000+ citations for Recombinant Mouse TNFRSF9 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Protein bands were visualized with the enhanced chemiluminescent HRP substrate Crescendo Immobilon (Millipore). Analysis was performed in ImageJ ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant containing total plant proteins was passed through Miracloth (Millipore, 475855-1R) and incubated with 100 μl of anti-FLAG M1 Agarose affinity gel (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... After destaining proteins in the gel bands were reduced with 20mM dithiothreitol (Sigma) for 1h at 56°C and then alkylated with 60mM iodoacetamide (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by pre-clearing with Magna ChIP Protein A+G magnetic beads (Millipore) for 2 hours rotating at 4C ...
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bioRxiv - Cell Biology 2024Quote: ... HeLa cells transfected with plasmids encoding nucleocapsid proteins were treated with sucrose (Sigma) at a concentration of 300 mM ...
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bioRxiv - Biochemistry 2024Quote: ... proteins were concentrated to appropriate concentrations using Amicon Ultra-15 Centrifugal Filters (Millipore).
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bioRxiv - Immunology 2024Quote: ... proteins were immobilized on a primed 0.45 µm polyvinylidene difluoride membrane (Merck-Millipore), stained with Direct blue 71 (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2024Quote: ... Purified protein was then concentrated in an Amicon 30 kDa centricon (Millipore, UFC9030), and buffer was exchanged for wash buffer B ...
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bioRxiv - Immunology 2024Quote: ... FhNEJ-Som protein pellets were resuspended in 50 mM ammonium bicarbonate (Sigma, #207861) and 1% sodium deoxycholate (Acros Organics ...
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bioRxiv - Neuroscience 2024Quote: Total protein was extracted from cultures using CelLytic M Lysis Buffer (Sigma-Aldrich) supplemented with Protease and Phosphatase Inhibitor Cocktail (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... Bead-bound proteins were eluted with 200 ng/µL 3’FLAG peptide (Sigma) by incubating at 4°C for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were reduced by adding 2.5 µl of 250 mM dithiothreitol (D0632, Sigma) and incubated at 45°C for 37 min while shaking at 400 rpm on a block heater ...
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bioRxiv - Molecular Biology 2024Quote: ... Successful protein transfer was confirmed by incubating membranes in Ponceau S solution (Sigma) for 5 minutes with rocking and membranes were blocked in 5% milk in TBS buffer containing 0.1% Tween-20 for 1 hour with gentle rocking ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were electrophoretically transferred to an Inmobilon-P polyvinylidene difluoride (PVDF) membrane (Millipore) using the Trans-Blot Turbo (Bio-Rad) ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was concentrated using a 30 K Amicon Ultra-4 concentrator (Millipore) and further purified by gel filtration chromatography on a Superdex 200 Increase 10/300 GL column (Cytiva ...
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bioRxiv - Neuroscience 2024Quote: ... glial fibrillary acidic protein (GFAP) (1:500; Millipore Sigma; Burlington, MA; Cat#: 345860)] in blocking solution overnight at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... These constructs were used for protein expression in Rosetta (DE3) competent cells (Novagen) cultured in TB as reported previously11,46,47 ...
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bioRxiv - Biochemistry 2024Quote: ... Protein eluate was further concentrated in a 50 kDa MWCO concentrator (Millipore Sigma). Concentrated protein was loaded onto the Superose 6 Increase 10/300 GL (Cytiva ...
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Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathybioRxiv - Developmental Biology 2024Quote: ... Proteins were transferred onto a 0.45 µm PVDF Immobilon-P membrane (Millipore, Merck) using the Pierce Power Blotter (Thermo Fisher) ...
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bioRxiv - Neuroscience 2024Quote: ... Aggregated protein was isolated from the samples using phosphotungstic acid (PTA; Sigma-Aldrich) as described.9 ...
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bioRxiv - Microbiology 2024Quote: ... All proteins were concentrated using a BioMax concentrator (EMD Millipore, Burlington, MA, USA) followed by passage through a 0.2-μm Ultrafree-MC centrifugal filtration device (EMD Millipore ...
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bioRxiv - Microbiology 2024Quote: ... parasitica lysates using the OxyBlot Protein Oxidation Detection kit (Millipore, Billerica, MA, USA). Carbonylated proteins were derivatized with 2,4 Dinitrophenyl hydrazine (DNP ...
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bioRxiv - Plant Biology 2024Quote: ... We transferred the proteins from the gel to an Immobilon-P membrane (Millipore) at 400mA for 2 hours ...
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Synaptic inhibition in the accessory olfactory bulb regulates pheromone location learning and memorybioRxiv - Neuroscience 2024Quote: ... the proteins were transferred to the Immobilon-P PVDF membranes (Millipore Cat # IPVH00010) through methanol buffer transfer ...
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bioRxiv - Neuroscience 2024Quote: ... and rabbit anti-microtubule-associated protein 2 (MAP2; 1:500, EMD Millipore, AB5622), overnight at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... Separated proteins were then transferred to PVDF (Fluor) membrane (Millipore, USA, Cat. #: IPVH00010) for (1 h at 100 V ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were separated by electrophoresis and transferred to pre-activated PVDF membranes (Millipore). The membranes were blocked in 5% milk or 5% BSA ...
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bioRxiv - Neuroscience 2024Quote: ... Protein bands were stained by incubating the gel in Coomassie Brilliant Blue (Sigma) for 20 min at room temperature on an orbital shaker ...
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bioRxiv - Cell Biology 2024Quote: Proteins were resolved in 10% SDS PAGE and transferred to PVDF membrane (Millipore, Burlington ...
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bioRxiv - Immunology 2024Quote: ... The proteins on the gel were then transferred to PVDF membranes (Millipore, IPVH00010), which were blocked in 5% BSA for 1 h at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to a 0.45µm Immobilon-P Membrane (Millipore Sigma cat. IPVH00010) in ice-cold Tris-Glycine Buffer with 20% methanol in a transfer tank either overnight at 10V or 1 hour at 50V ...
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bioRxiv - Molecular Biology 2024Quote: ... the supernatant was filtered through a 0.45 µm low protein binding membrane (Millipore). And then ...
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bioRxiv - Molecular Biology 2024Quote: ... Eluted proteins were concentrated and washed twice with dH2O using Centricon 50 (Millipore) filtration devices.
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bioRxiv - Molecular Biology 2024Quote: ... Protein G magnetic beads used for immunoprecipitation were from Millipore (Billerica, MA, USA).
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bioRxiv - Molecular Biology 2024Quote: ... we inserted the yellow fluorescent protein (YFP) gene into the pET22b(+) plasmid (Novagen) as follows ...
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bioRxiv - Microbiology 2024Quote: ... The protein was concentrated using Amicon centrifugal units with 10kDa pore size (Millipore), the final protein concentration was evaluated by Bradford assay and protein purity was assessed by SDS-PAGE ...
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bioRxiv - Cancer Biology 2024Quote: ... Supernatants containing chromatin fragments were pre-cleared with protein A agarose beads (Millipore) for 40 min and incubated with a specific antibody overnight at 4 °C on a rotator (antibody information is listed in Supplementary Table 2) ...
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bioRxiv - Molecular Biology 2024Quote: ... The separated proteins were transferred onto a polyvinylidene difluoride (PVDF) membrane (Millipore IPFL00010) using phosphate-based transfer buffer (10 mM sodium phosphate monobasic ...
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bioRxiv - Molecular Biology 2024Quote: ... The dialyzed protein was concentrated using Amicon centrifugal filters (Millipore, Burlington, MA, USA) with a molecular weight cutoff of 10 kDa ...
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bioRxiv - Biochemistry 2024Quote: ... Protein expression was enhanced by treating with 10 mM sodium butyrate (Sigma-Aldrich) after 12-16 hours of infection and further incubated for 48 to 60 hours ...
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bioRxiv - Biochemistry 2024Quote: ... 0.5 mM TCEP) and concentrated with a 30 K MWCO protein concentrator (Millipore). Proteins were snap-frozen in liquid nitrogen and stored at −80 °C until use.
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bioRxiv - Bioengineering 2024Quote: ... Protein concentration in the membrane fraction was determined by Bradford assay (Sigma-Aldrich), and 5 μg of total proteins were subjected to SDS-PAGE ...
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bioRxiv - Biophysics 2024Quote: ... Cleaved proteins were filtered by centrifuging with Ultrafree-MC VV filter (EMD Millipore) in a tabletop centrifuge and flash frozen in liquid nitrogen.
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bioRxiv - Cancer Biology 2024Quote: ... The primary antibody or IgG and protein A or G beads (Millipore Corp.) were added to the lysates ...
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bioRxiv - Biophysics 2024Quote: ... The eluted protein was concentrated using a 3K MWCO centrifugal filter (Sigma-Aldrich) and run through SEC on a Superdex 200 10/300 column (Cytiva ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatant was filtered through a 0.45 μm low protein binding membrane (Millipore) and used immediately after supplementing with 7μg/ml of polybrene or stored in -80°C ...
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bioRxiv - Biochemistry 2024Quote: ... The proteins were then alkylated with 1mM p-Nitrobenzyl mesylate (PNBM, Sigma Aldrich) and incubated further at room temperature for 2 hours in the dark ...
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Biosynthetic characterization and combinatorial biocatalysis of the cysteine protease inhibitor E-64bioRxiv - Biochemistry 2024Quote: ... was used to calculate protein concentration with bovine serum albumin (BSA, Sigma-Aldrich) as standard ...
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bioRxiv - Biochemistry 2024Quote: ... Protein expression was enhanced by treating with 10 mM sodium butyrate (Sigma-Aldrich) after 12-16 hours of infection and further incubated for 48 to 60 hours ...
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bioRxiv - Bioengineering 2024Quote: ... Purified proteins were concentrated with 30 kDa cut-off filters (Amicon, Merck Millipore) at 4 °C ...