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Citations for Millipore Sigma :
8951 - 9000 of 10000+ citations for Recombinant Mouse TNFRSF9 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2019Quote: ... Co-IP assays were performed by incubating total proteins with anti-HA (Sigma) agarose followed by column wash and elution according to manufacturer’s instructions (Pierce ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The equal loading of the blotted protein was verified by Ponceau S (Sigma) staining ...
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bioRxiv - Biochemistry 2019Quote: ... and 1% NP-40 containing protein phosphatase inhibitor cocktail 1 and 2 (Sigma) and protease inhibitors (Sigma)] ...
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bioRxiv - Cell Biology 2019Quote: ... after which the proteins were transferred to a PVDF membrane (Immobilon-P, Millipore). Primary antibodies were incubated overnight at 4 °C in 1% milk ...
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bioRxiv - Biophysics 2019Quote: ... Fractions containing the purified proteins were concentrated using Amicon Ultra concentrators (Millipore Sigma).
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bioRxiv - Synthetic Biology 2020Quote: ... Total protein concentration in CFE was determined by Bradford reagent (Sigma-Aldrich, USA), and ∼55 mg of total protein in CFE was incubated for 60 min at 4 °C with 2 mL of Ni-NTA agarose (QIAGEN ...
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bioRxiv - Microbiology 2019Quote: ... The protein concentration in the extract was determined with a Bradford assay (Sigma) and used for normalisation of the SOD activity.
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bioRxiv - Cell Biology 2020Quote: ... Protein A or G agarose (Pierce, 20333 or Millipore-Sigma 16-201, respectively) was equilibrated in lysis buffer and added to the lysates at 4°C with gentle rotation ...
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bioRxiv - Cell Biology 2020Quote: ... Protein A or G agarose (Pierce, 20333 or Millipore-Sigma 16-201, respectively) was equilibrated in lysis buffer and added to the lysates at 4°C with gentle rotation ...
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bioRxiv - Developmental Biology 2019Quote: ... Proteins were detected with the primary antibodies against Flag (1:1000, Sigma F7425), GFP (1:1000 ...
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bioRxiv - Systems Biology 2019Quote: ... Proteins were precipitated with 1 μg of anti-Flag antibody (F-3165, Sigma) for 2 h at 4°C followed by the addition of 15 μl protein G-Sepharose beads for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... The total nuclear protein was lysed with CHAPS buffer containing protease inhibitor (Sigma) and phosphatase inhibitor (Roche Applied Science ...
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bioRxiv - Plant Biology 2021Quote: ... Sonicated samples were first precleared with Protein A Agarose beads (Millipore #16-157) and then mixed with antibodies ...
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bioRxiv - Molecular Biology 2020Quote: ... TAP-tagged proteins were detected with peroxidase–anti-peroxidase– soluble complex (P1291, Sigma). α-tubulin was detected with antibody T5168 (Sigma).
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bioRxiv - Microbiology 2020Quote: ... The protein-DNA complex was treated with 1 U/μl DNase I (Novagen) to digest non-specific DNA ...
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bioRxiv - Immunology 2021Quote: ... Protein bands were revealed by enhanced chemiluminescence reagents (ECL™ Prime, Merck Millipore) in a ChemiDoc XRS+ imaging system (BioRad ...
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bioRxiv - Synthetic Biology 2021Quote: ... Protein was precipitated by adding 1 volume of TCA (trichloroacetic acid, Sigma Aldrich) to 4 volumes of supernatant ...
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bioRxiv - Microbiology 2020Quote: ... protein containing fractions were pooled and concentrated with an Amicon Ultracel-10K (Millipore) according to the experimental requirements.
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bioRxiv - Microbiology 2020Quote: ... Proteins in the supernatants were precipitated with 4x volume cold acetone (V800023, Sigma) overnight at −20°C ...
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bioRxiv - Molecular Biology 2021Quote: ... or proinsulin Ab (CCI-17) bound protein G beads (GE17-0618-01, Sigma) for overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... and proteins were transferred onto polyvinylidene difluoride (PVDF) membranes (Merck Millipore, Chengdu, China) for 2 h at 150 mA in 10% transfer buffer [3% Tris–HCl (Aladdin Industrial Corporation ...
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bioRxiv - Neuroscience 2020Quote: ... A mixture of primary antibodies against enhanced green fluorescent protein (EGFP: chicken, Millipore), human influenza hemagglutinin (HA ...
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bioRxiv - Biochemistry 2020Quote: ... Tie2 protein was immunoprecipitated from the lysates by using anti‐Tie2 antibody (Millipore) and protein A/G‐sepharose ...
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bioRxiv - Neuroscience 2020Quote: ... depending on respective protein molecular weight) and transferred on a PVDF membrane (Millipore). Blots were blocked with 5% milk powder or 5% BSA in TBS + 0.1% Tween-20 (TBST ...
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bioRxiv - Immunology 2020Quote: ... MaxiSorp plates were coated with 1 μg/mL HIV-GP120 protein (Sigma-Aldrich). Samples were diluted in an eight-point two-fold dilution series ...
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bioRxiv - Microbiology 2021Quote: ... The lysate was mixed with 40 μL protein G agarose (Millipore, United States) to avoid nonspecific binding for 2 h at 4 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein concentration was measured using a standard Bradford assay reagent (B6916, Sigma Aldrich).40µg total protein were loaded per samples on a pre-cast gradient 4-12% gel (NW04120 ...
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bioRxiv - Molecular Biology 2019Quote: ... The proteins were concentrated using Amicon Ultracel-30 Centrifugal Filter Units (Merck Millipore) for wild-type RF2 and RF2-GGP and Ultracel-3 for ResQ62-His6 hereafter referred to as ResQ.
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bioRxiv - Microbiology 2019Quote: ... Bound His-tagged proteins were detected using HRP-conjugated anti-His antibodies (Sigma) at a 1:500 dilution ...
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bioRxiv - Immunology 2019Quote: ... 6 μl (=10 μg) total protein was then reacted with streptavidin (Sigma, S4762) in PBS (total reaction volume 24 μl ...
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bioRxiv - Molecular Biology 2019Quote: Proteins were separated on 5.5% polyacrylamide gel and transferred on PVDF membrane (Millipore) using semi-dry blotting for 50 min ...
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bioRxiv - Genetics 2020Quote: Proteins were reduced with adding 1.5 μl of 200 mM dithiothreitol (DTT, Sigma) in 500 mM AmBic and incubated at 37 °C for 1 h with shaking at 400 rpm ...
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bioRxiv - Cell Biology 2020Quote: ... Myc-tagged proteins were immunoprecipitated with 4AG Myc monoclonal agarose beads (EMD Millipore), washed three times in lysis buffer and boiled in 2X Laemmli reducing buffer (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to a Immobilion-P PVDF membrane (Millipore Sigma, MA, USA) at 47V overnight at 4°C using Mini Trans-Blot® Cell (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2020Quote: ... Fractions containing protein were concentrated in 50 kDa cutoff Amicon centrifugal filters (Millipore). Aliquots were frozen in dry ice and stored at −80 °C.
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bioRxiv - Molecular Biology 2020Quote: ... fractions containing protein were concentrated in 10 kDa cutoff Amicon centrifugal filters (Millipore). Aliquots were frozen in dry ice and stored at −80 °C.
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bioRxiv - Neuroscience 2021Quote: ... Proteins were detected by the ECL method (Immobilon Western Chemiluminescent HRP Substrate, Millipore), and loading control of the western blot membrane was performed using the Revert total protein stain (LI-COR Bioscience).
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated by SDS-PAGE and transferred onto a PVDF membrane (Millipore). The following antibodies were used for immunoblotting ...
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bioRxiv - Cell Biology 2020Quote: ... The protein was then transferred onto a polyvinylidene difluoride membrane (Millipore, Toronto, Canada) and probed using antibodies for PI4KIIIβ (BD Biosciences 611817 ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were buffer exchanged into 200 mM ammonium acetate buffer pH 7.4 (Sigma), supplemented with 0.02% DDM for ACKR1 and for toxins ...
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bioRxiv - Biochemistry 2021Quote: ... Protein was then loaded onto a 20 ml chelating Nickel HiTrap column (Sigma) that had been equilibrated in Ni column binding buffer ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were buffer exchanged into 200 mM ammonium acetate buffer pH 7.4 (Sigma) using Bio-Spin microcentrifuge columns (Bio-Rad Laboratories) ...
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bioRxiv - Neuroscience 2021Quote: ... Separated proteins were transferred to methanol-activated Immobilon-P PVDF membrane (Millipore IPVH00010) for 90 minutes at 25V at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... 750µg total protein was then incubated with 20µg prewashed FLAG affinity gel (Sigma) for 2h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... and for utrophin samples were quantified against total protein using Coomassie stain (Sigma).
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bioRxiv - Biochemistry 2021Quote: ... All proteins were expressed in Escherichia coli BL21(DE3) Rosetta/pLysS strain (Novagen). Cells were grown in LB medium supplemented with 10% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... The protein-containing cell culture supernatant was filtered through 0.22μm PES membranes (Millipore), concentrated using VIVAFLOW 50R (Sartorius ...
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bioRxiv - Microbiology 2020Quote: ... The protein-containing cell culture supernatant was filtered through 0.22μm PES membranes (Millipore) and passed over 0.5ml of a Strep-Tactin Superflow (IBA Lifesciences ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... antimicrotubule-associated protein 2 (MAP2) Alexa Fluor 647 (1:500, NB120-11267AF647; Millipore). Cell nuclei were stained by using Hoescht 33342 (2 μg/mL ...
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bioRxiv - Synthetic Biology 2021Quote: ... The eluted proteins were concentrated using Amicon® Ultra Centrifugal Filters (Millipore Sigma). The concentrated proteins were dialyzed into SUMO-SIM protein buffer (150 mM KCl ...