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Citations for Millipore Sigma :
8901 - 8950 of 10000+ citations for Recombinant Mouse TNFRSF9 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... Actin protein was detected with anti-Actin antibody (Millipore-Sigma MAB1501, 1:3000). For other immunoblotting ...
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bioRxiv - Molecular Biology 2023Quote: ... Fractions containing protein were concentrated in 50-kDa cutoff Amicon centrifugal filters (Millipore). Aliquots were flash frozen in liquid nitrogen and stored at −80 °C.
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bioRxiv - Molecular Biology 2023Quote: ... fractions containing protein were concentrated in 10-kDa cutoff Amicon centrifugal filters (Millipore). Aliquots were flash frozen in liquid nitrogen and stored at −80 °C.
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bioRxiv - Biophysics 2023Quote: ... The eluted protein was concentrated in a 50 kDa cut-off Vivaspin (Millipore) and run on a Superose 6 size exclusion column (GE Healthcare ...
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bioRxiv - Cancer Biology 2023Quote: ... and 50 µM Rho-associated protein kinase (ROCK) inhibitor (Merck Millipore, SCM-075). On day 3 ...
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bioRxiv - Biophysics 2023Quote: ... the protein was digested online by a pepsin/FPXIII (Sigma-Aldrich P6887/P2143) mixed protease column maintained at 20 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein samples were then concentrated using Amicon Ultra centrifugal filters (30k MWCO, Millipore). The protein concentration of eluates were estimated with a Bradford assay (BioRad ...
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bioRxiv - Cell Biology 2023Quote: ... proteins from the gel were transferred onto a PVDF membrane (Millipore, cat#IPVH00010). The membrane was subsequently blocked and incubated with primary and secondary antibodies ...
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bioRxiv - Pathology 2023Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated in the blocking buffer (5% non-fat milk ...
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bioRxiv - Cancer Biology 2023Quote: ... the protein was concentrated using an 10K MWCO centrifugal concentrator (Amicon-15, Millipore) to 20 mg/mL and purified by size exclusion chromatography on a Superdex 75 10/300 GL column (GE Healthcare Life Sciences) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Proteins were then precipitated upon addition of 50% trichloroacetic acid (TCA, Sigma-Aldrich) to a final concentration of 5% ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins coupled to streptavidin-coated magnetic beads were reduced with dithiothreitol (Sigma-Aldrich) and alkylated with iodoacetamide ...
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bioRxiv - Physiology 2023Quote: ... Proteins were then concentrated to approximately 3 mg/ml by ultrafiltration (Millipore, UFC905096) at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... purified WT or ΔFN2 proteins were incubated with rat tail collagen (EMD Millipore) and then co-IP was carried out as described above.
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bioRxiv - Plant Biology 2023Quote: ... The protein eluent was concentrated using a 100-kDa cut-off Centricon (Millipore) and subsequently loaded onto a Superose 6 Increase 5/150 GL column (Cytiva ...
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bioRxiv - Pathology 2023Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated in the blocking buffer (5% non-fat milk ...
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bioRxiv - Immunology 2023Quote: ... Total protein concentration was determined using the QuantiPro BCA assay kit (Sigma-Aldrich) by first interpolating the calibration curve using BSA standard solution (0.5 – 30 μg/ml ...
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bioRxiv - Immunology 2023Quote: ... aliquots of protein were digested twice (overnight and 4h) with trypsin (Sigma-Aldrich) at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were concentrated by centrifugal filters (Amicon Ultra 10K, Merck Millipore Ltd.). Protein concentrations were determined by a Pierce™ BCA Protein Assay Kit (Thermo Fischer Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Purified protein was concentrated by centrifugal ultrafiltration using Amicon Ultra 30 K (Millipore) and quantified spectrophotometrically at 280 nm (mass extinction coefficient E 1% ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were buffer-exchanged against 100 mM ammonium acetate buffer pH 7.4 (Sigma) using Amicon centrifugal filters (Merck ...
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bioRxiv - Biochemistry 2023Quote: ... the proteins were filtered using an Ultrafree 0.22 μm centrifugal filter (Merck Millipore) to remove any aggregation ...
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bioRxiv - Cell Biology 2023Quote: ... protein was extracted from SC cells using lysis buffer (CelLyticTM M, Sigma-Aldrich) supplemented with Halt™ protease/phosphatase inhibitor cocktail (Thermo Fisher Scientific) ...
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Mitochondrial Apolipoprotein MIC26 is a metabolic rheostat regulating central cellular fuel pathwaysbioRxiv - Cell Biology 2023Quote: ... proteins were transferred onto nitrocellulose membranes and stained using Ponceau S (Sigma Aldrich). After destaining ...
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bioRxiv - Cell Biology 2023Quote: ... the proteins were transferred to 0.45 µm PVDF membranes (Merck Millipore, cat.no. IPVH00010). After blocking unspecific binding sites of the membrane at RT for 1 h with 5 % skim milk powder in TBS-T ...
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bioRxiv - Cell Biology 2023Quote: ... 1% NP-40) supplemented with a protein inhibitor cocktail 1:100 (Sigma-Aldrich). Samples denatured by 3 min boiling in Laemmli buffer were separated by electrophoresis on 4-12% bis-acrylamide gel (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins in samples were detected using Immobilon Western Chemiluminescent HRP Substrate (Sigma-Aldrich) and image acquisition was performed on a G-Box (Ozyme) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein estimation was performed using BCA method as per the manufacturing protocol (Sigma). 20µg of protein from each sample prepared in 1x protein loading dye was used for polyacrylamide gel electrophoresis (Biorad) ...
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bioRxiv - Cell Biology 2023Quote: ... The immunoprecipitated TFEB proteins were dephosphorylated by λ phosphatase (Cat# P9614, Sigma-Aldrich) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Protein was then transferred onto a 0.45 µm PVDF membrane (Millipore, Cat. IPFL85R), blocked with 5% non-fat dried milk in TBS plus 0.01% Tween (TBST ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions containing protein were concentrated in 50-kDa-cutoff Amicon centrifugal filters (Millipore). Aliquots were frozen in liquid nitrogen and stored at −80 °C.
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bioRxiv - Cell Biology 2023Quote: Protein samples were separated by SDS-PAGE and transferred onto PVDF membrane (Millipore) by semi-dry blotting using 1x Tris-glycine buffer (192 mM glycine ...
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bioRxiv - Microbiology 2023Quote: ... 50 μl of slurry of Magna ChIP Protein G magnetic beads (Sigma-Aldrich) were washed twice with IP/wash buffer 20 mM Tris HCl pH 7.4 ...
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bioRxiv - Developmental Biology 2023Quote: ... 40 μl of Protein A Sepharose CL-4B beads (Sigma; GE17-0780-01) was added in the tube and incubated for and additiona1 hour at 4°C under rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted with 5 ml of 1 mg/ml Flag-peptide (Sigma), followed by the addition of 20 ml of QA buffer (50mM TRIS pH 7.7 ...
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bioRxiv - Neuroscience 2024Quote: ... glial fibrillary acidic protein (GFAP) (1:500; Millipore Sigma; Burlington, MA; Cat#: 345860)] in blocking solution overnight at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were electrophoretically transferred to an Inmobilon-P polyvinylidene difluoride (PVDF) membrane (Millipore) using the Trans-Blot Turbo (Bio-Rad) ...
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bioRxiv - Cancer Biology 2024Quote: ... Biotin switch-treated protein reduced with 2.5 μL of 0.2 M dithiothreitol (Sigma) for 1 hr at 57°C and the samples cooled before adding 2.5 μL of 0.5M iodoacetamide and incubated in the dark for 45 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was concentrated using a 30 K Amicon Ultra-4 concentrator (Millipore) and further purified by gel filtration chromatography on a Superdex 200 Increase 10/300 GL column (Cytiva ...
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bioRxiv - Cancer Biology 2024Quote: Protein lysates were extracted from flash-frozen pellets with RIPA buffer (Sigma R0278), supplemented with protease and phosphatase inhibitors (Invitrogen A32961) ...
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bioRxiv - Microbiology 2024Quote: ... parasitica lysates using the OxyBlot Protein Oxidation Detection kit (Millipore, Billerica, MA, USA). Carbonylated proteins were derivatized with 2,4 Dinitrophenyl hydrazine (DNP ...
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bioRxiv - Biochemistry 2024Quote: ... Protein eluate was further concentrated in a 50 kDa MWCO concentrator (Millipore Sigma). Concentrated protein was loaded onto the Superose 6 Increase 10/300 GL (Cytiva ...
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Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathybioRxiv - Developmental Biology 2024Quote: ... Proteins were transferred onto a 0.45 µm PVDF Immobilon-P membrane (Millipore, Merck) using the Pierce Power Blotter (Thermo Fisher) ...
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bioRxiv - Microbiology 2024Quote: ... All proteins were concentrated using a BioMax concentrator (EMD Millipore, Burlington, MA, USA) followed by passage through a 0.2-μm Ultrafree-MC centrifugal filtration device (EMD Millipore ...
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bioRxiv - Molecular Biology 2024Quote: ... Successful protein transfer was confirmed by incubating membranes in Ponceau S solution (Sigma) for 5 minutes with rocking and membranes were blocked in 5% milk in TBS buffer containing 0.1% Tween-20 for 1 hour with gentle rocking ...
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bioRxiv - Bioengineering 2024Quote: ... These constructs were used for protein expression in Rosetta (DE3) competent cells (Novagen) cultured in TB as reported previously11,46,47 ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were detected by immunoblotting with anti-Cdh1 antibodies (1 µg/ml, Millipore) and anti-GST antibodies (0.5 µg/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... Lysates were pre-cleared using protein G agarose beads (16-266, Sigma Aldrich). The pre-cleared lysates were incubated with streptavidin-agarose beads (20359 ...
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bioRxiv - Neuroscience 2024Quote: ... Aggregated protein was isolated from the samples using phosphotungstic acid (PTA; Sigma-Aldrich) as described.9 ...
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bioRxiv - Microbiology 2024Quote: ... Proteins in the supernatant were precipitated with 1:4 trichloroacetic acid (Sigma Aldrich). The pellet was washed and disrupted with acetone two times and re-dissolved in 200 mM ammonium bicarbonate with 6 M Urea (Sigma Aldrich) ...