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Citations for Millipore Sigma :
8901 - 8950 of 10000+ citations for Recombinant Mouse TNFRSF9 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... 8 μg of purified proteins were incubated with 1 mM DMAPP (Sigma-Aldrich) and 1 mM AMP or ATP for two hours at 30°C in 12.5 mM Tris–HCl (pH 7.5) ...
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bioRxiv - Neuroscience 2020Quote: ... The protein bands were detected using 3,3 ́-diaminobenzidine tetrahydrochloride hydrate (DAB) (Sigma, D5637). The blots were incubated in a solution containing 1mg/ml DAB in 50 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... The proteins were separated by electrophoresis and transferred to polyvinylidene fluoride membranes (Millipore). The membranes were incubated with appropriate mouse antibodies directed against TLR4 (ab22048 ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was collected and protein concentration was measured by Bradford assay (Sigma).
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bioRxiv - Developmental Biology 2021Quote: ... proteins were precipitated twice by addition of 1 mg of gamma-globulins (Sigma) and 2 ml 20% TCA followed by centrifugation at 2,000 r.p.m ...
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bioRxiv - Biochemistry 2021Quote: ... The purified proteins were quantified through a Bradford assay kit (Sigma -Aldrich, USA). The purified proteins were running in 2-D gel electrophoresis and images were taken and analyzed in gel dock(Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... protein was then loaded on to Fractogel EMD SO3- cation exchange column (Millipore) in 20 mM MES ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were detected via western blotting with M2 anti-FLAG (Sigma-Aldrich, F1804) and THE anti-Strep II tag FITC (Genescript ...
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bioRxiv - Neuroscience 2021Quote: ... To elute proteins from the beads a 3xFLAG peptide (100 ug/mL, Sigma) with 0.05% RapiGest in IP buffer was added to the beads for 15 minutes at room temperature with agitation ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was concentrated using a 30 kDa cutoff centrifugal filter unit (Millipore) and chromatographed on a Superdex 200 increase column in a final buffer of 20 mM HEPES pH 7.0 ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were competitively eluted using 3X FLAG peptide (Sigma; in Myc wash buffer). The eluted FLAG-ACBD5 was incubated with the Myc-VAPB-bound beads for 1 h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were visualized using HRP-conjugated secondary antibody and chemiluminescent HRP substrate (Millipore).
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bioRxiv - Cell Biology 2021Quote: Wash 20μl Magna ChIP™ Protein A+G Magnetic Beads (Millipore, 16-663) twice with 1 ml FA buffer (10mM HEPES[PH7.5],150mM NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... Protein samples were incubated with primary antibodies against Foxp4 (Millipore, #ABE74, 1:1000), Ucp1 (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... The purified proteins were further concentrated on Amicon Ultra Centrifugal Filters (Sigma-Aldrich) and quantified using Qubit protein assay.
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bioRxiv - Microbiology 2020Quote: ... The eluted protein was concentrated using a 10 kDa MWCO filter (Amicon-Millipore), and concentrated up to 2 mg/mL.
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bioRxiv - Neuroscience 2021Quote: ... Proteins were then transferred to an Immobilon-FL PVDF membrane (Millipore, Billerica, MA) on ice either for 2 h at 100 V ...
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bioRxiv - Immunology 2020Quote: ... Cell lysates were incubated with antibody and protein A/G magnetic beads (Sigma) from 3 h to overnight at 4°C under rotation conditions ...
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bioRxiv - Cancer Biology 2020Quote: ... 60 μL of Magna ChIP™ Protein A+G Magnetic Beads (Merck Millipore) was used in accordance with the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... the separated proteins were transferred onto 0.45 μm polyvinylidene difluoride membranes (Millipore, USA). Next ...
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bioRxiv - Cancer Biology 2021Quote: ... denatured proteins were loaded onto 30 kDa amicon filter (Merck Millipore, Darmstadt, Germany). The buffer was exchanged with UA solution (8 M UREA in 0.1 M TEAB pH 8.5 ...
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bioRxiv - Biochemistry 2021Quote: ... imidazole was removed and the protein was concentrated using a Centricon (Merck Millipore) and preserved at −80 °C in small fractions at 2 mM concentration.
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bioRxiv - Neuroscience 2020Quote: ... Proteins separated in the gel were transferred to a PVDF membrane (Merck Millipore) using a submerged blotting apparatus and then visualized using Immobilon Western Chemiluminescent HRP Substrate (Millipore) ...
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bioRxiv - Biophysics 2020Quote: ... The CbAgo protein was expressed in E.coli Bl21(DE3) Rosetta™ 2 (Novagen). Cultures were grown at 37 °C in LB medium containing 50 μg ml−1 kanamycin and 34 μg ml−1 chloramphenicol till an OD600 nm of 0.7 was reached ...
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bioRxiv - Cancer Biology 2021Quote: ... Co-Immunoprecipitation was performed using PureProteome™ Protein A Magnetic Beads (Millipore, LSKMAGA10). Protein extracts were first precleared using 10μl of beads for 200μg of proteins (1-hour incubation at 4°C) ...
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bioRxiv - Cancer Biology 2021Quote: ... The protein was also visualized using 4-Chloro-1-naphthol solution (Sigma Aldrich) which is a substrate for HRP in a reaction that results in a colored precipitate.
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bioRxiv - Plant Biology 2021Quote: ... GST-PPKs were tested against 5 µg myelin basic protein (MBP; Sigma Aldrich) and 5 µg Casein in 0.1 M Tris pH 8.8 (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-purkinje cell protein 4 (PCP4, 1:500, Sigma-Aldrich, Cat# HPA005792), rat anti-mCherry (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... resolved proteins were further transferred to a PVDF membrane (0.45 μm; Millipore, USA) for immunoblotting and the blots were blocked using Starting Block T20 (TBS ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein was concentrated using a 50-kDa cutoff Amicon filter (Sigma-Aldrich) and purified on a Superose 6 Increase 10/30 gel filtration column (GE Healthcare) ...
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bioRxiv - Neuroscience 2022Quote: ... Protein was extracted using 100 µL of lysis buffer (7 M urea (Sigma), 2 M thiourea (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: Proteins were transferred to 0.45 μm Immobilon-P PVDF membrane (Millipore, Burlington, MA) in transfer buffer (25 mM Tris ...
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bioRxiv - Immunology 2022Quote: ... The immunocomplex was captured by adding Protein G Agarose (Merck Millipore, Darmstadt, Germany). The protein binding to the beads was boiled in 2 x Laemmli sample buffer (Bio–Rad ...
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bioRxiv - Genetics 2022Quote: ... The protein solution was loaded onto glass slides within a silicone isolator (Sigma) and imaged using a Zeiss LSM 800 ...
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bioRxiv - Cell Biology 2022Quote: ... proteins were transferred onto a PVDF membrane (Immobilon®-P Membrane, Millipore #IPVH00010) (120mA/gel ...
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bioRxiv - Immunology 2022Quote: ... protein bands were stained with silver nitrate (ProteoSilver Silver Stain Kit, Sigma-Aldrich). The two recombinant proteins were analyzed in non-denaturing conditions on a Superose 6 10/300 GL size exclusion chromatography (SEC ...
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bioRxiv - Cancer Biology 2022Quote: ... and the proteins were detected with the following antibodies: Flag (Sigma-Aldrich; F7425), GFP (Abcam ...
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bioRxiv - Cell Biology 2022Quote: ... for 8 h with protein A/G agarose beads (Sigma-Aldrich, Burlington, Massachusetts) at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... 10μg of antibody was coupled to 50μl packed Protein A beads (Sigma P9424) by incubation in 1ml PBS (0.1% Tween-20 ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein was concentrated using a 3 kDa cutoff spin concentrator (Millipore Sigma UFC9003) to a volume of 2 mL ...
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bioRxiv - Plant Biology 2022Quote: ... 50 µg GST–CAR9 protein emulsified with Incomplete Freund’s adjuvant (F5506, Sigma–Aldrich) was injected into the ICR mouse and then once again in the next week ...
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bioRxiv - Biochemistry 2019Quote: ... The purified protein was concentrated with an Amicon Ultra-10 (Millipore, Billerica, MA) for future NMR study ...
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Removal of Spo11 from meiotic DNA breaks in vitro but not in vivo by Tyrosyl DNA Phosphodiesterase 2bioRxiv - Molecular Biology 2019Quote: ... and Spo11 was immunoprecipitated from total soluble protein using anti-FLAG antibody (Sigma) and protein-G-agarose (Roche) ...
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bioRxiv - Biophysics 2019Quote: ... The CbAgo protein was expressed in E.coli Bl21(DE3) Rosetta™ 2 (Novagen). Cultures were grown at 37°C in LB medium containing 50μa.g ml-1 kanamycin and 34μg ml-1 chloramphenicol till an ODóOOnm of 0.7 was reached ...
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bioRxiv - Molecular Biology 2019Quote: ... plasmids for different Cas9 proteins were expressed in Escherichia coli Rosetta2 (DE3) (Novagen). The protein expressing Rosetta2 (DE3 ...
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bioRxiv - Neuroscience 2019Quote: ... Proteins were then transferred to an Immobilon-FL PVDF membrane (Millipore, Billerica, MA) on ice either for 2 h at 100 V ...
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bioRxiv - Molecular Biology 2019Quote: ... Myc-tagged proteins were incubated with an anti-Myc primary antibody 9E10 (Millipore) at a 1:2000 dilution while tubulin was incubated with anti-Tubulin antibody B-5-1-2 (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2019Quote: ... the protein was transferred to a polyvinylidene difluoride (PVDF) membrane (Immobilon-P; Millipore). The membrane was blocked with 5% skimmed milk and incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... Immunoprecipitated protein/DNA complexes were prepared using the Magna ChIP A kit (Millipore) following manufacture’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Proteins were extracted by homogenizing the tissue in ice-cold RIPA buffer (Sigma) and lysates were centrifuged at 16,000 × g at 4°C ...