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Citations for Millipore Sigma :
8851 - 8900 of 10000+ citations for Recombinant Mouse TNFRSF9 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: Proteins in 20 µL solution were precipitated with trichloroacetic acid (TCA; Sigma-Aldrich) at a final concentration of 5% and washed twice with ice-cold acetone ...
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bioRxiv - Biochemistry 2023Quote: ... the proteins were filtered using an Ultrafree 0.22 μm centrifugal filter (Merck Millipore) to remove any aggregation ...
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bioRxiv - Cell Biology 2023Quote: ... the proteins were transferred to 0.45 µm PVDF membranes (Merck Millipore, cat.no. IPVH00010). After blocking unspecific binding sites of the membrane at RT for 1 h with 5 % skim milk powder in TBS-T ...
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bioRxiv - Cell Biology 2023Quote: ... 1% NP-40) supplemented with a protein inhibitor cocktail 1:100 (Sigma-Aldrich). Samples denatured by 3 min boiling in Laemmli buffer were separated by electrophoresis on 4-12% bis-acrylamide gel (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins in samples were detected using Immobilon Western Chemiluminescent HRP Substrate (Sigma-Aldrich) and image acquisition was performed on a G-Box (Ozyme) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein estimation was performed using BCA method as per the manufacturing protocol (Sigma). 20µg of protein from each sample prepared in 1x protein loading dye was used for polyacrylamide gel electrophoresis (Biorad) ...
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bioRxiv - Physiology 2023Quote: ... Protein concentration was determined using a bicinchoninic acid kit (BCA, Sigma, BCA1-1KT) according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Actin protein was detected with anti-Actin antibody (Millipore-Sigma MAB1501, 1:3000). For other immunoblotting ...
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bioRxiv - Pathology 2023Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated in the blocking buffer (5% non-fat milk ...
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bioRxiv - Cell Biology 2023Quote: ... Eluted proteins were concentrated using a 10K Amicon tube (Merck-Millipore, Darmstadt, Germany) and the buffer was changed to PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Eluted proteins were concentrated using a 10K Amicon tube (Merck-Millipore, Darmstadt, Germany) and the buffer was changed to PBS ...
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bioRxiv - Microbiology 2023Quote: ... The purified protein was concentrated to 5 mg/ml by ultrafiltration (Millipore, UFC901096) and maintained in 20 mM Tris-HCl ...
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bioRxiv - Immunology 2023Quote: ... The purified mini-HA protein was concentrated by Amicon spin filter (Millipore Sigma) and filtered by 0.22 µm centrifuge tube filters (Costar) ...
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bioRxiv - Immunology 2023Quote: ... Protein was then transferred onto a 0.45 µm PVDF membrane (Millipore, Cat. IPFL85R), blocked with 5% non-fat dried milk in TBS plus 0.01% Tween (TBST ...
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bioRxiv - Microbiology 2023Quote: ... 50 μl of slurry of Magna ChIP Protein G magnetic beads (Sigma-Aldrich) were washed twice with IP/wash buffer 20 mM Tris HCl pH 7.4 ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins from the gels were transferred onto Immobilon-P polyvinylidene fluoride (PVDF, Millipore) membranes for 90 minutes at 70 V ...
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bioRxiv - Plant Biology 2023Quote: ... Actin protein was detected with anti-Actin antibody (Millipore-Sigma MAB1501, 1:3000). For other immunoblotting ...
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bioRxiv - Neuroscience 2023Quote: ... Chicken anti-Glial Fibrillary Acidic Protein (GFAP) (1:500, Millipore, Cat# AB5541, RRID:AB_177521), Rabbit anti-Homer1 (1:1,000 ...
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bioRxiv - Developmental Biology 2023Quote: ... 40 μl of Protein A Sepharose CL-4B beads (Sigma; GE17-0780-01) was added in the tube and incubated for and additiona1 hour at 4°C under rotation ...
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bioRxiv - Plant Biology 2024Quote: ... HA-tagged proteins were detected by anti-HA Peroxidase high affinity antibody (Sigma). GFP was detected using a horseradish peroxidase-conjugated anti-GFP antibody (1:1.000 ...
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bioRxiv - Immunology 2024Quote: ... 5 mg purified RIPR protein fragment was incubated with TEV protease (Sigma/Promega) at a 1:10 v/v ratio overnight at 4 °C on a rolling mixer ...
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bioRxiv - Immunology 2024Quote: ... or anti-VSV matrix protein antibody (1:5,000 dilution, Sigma-Aldrich, Cat. #MABF2347) and subsequently with HRP-conjugated goat anti-mouse IgG (1:750 dilution ...
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bioRxiv - Cancer Biology 2022Quote: ... The proteins were separated in SDS-PAGE and transferred to PVDF membrane (Millipore) and subsequently probed with respective antibodies including anti-RAD51 (8875S ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein was then concentrated in an Amicon Ultra centrifugal filter (Millipore Sigma) to less than 2 mL total volume ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were transferred to Immobilon-P PVDF membrane (Cat. #PVH00010 Millipore, Bedford, MA) as described (Popova et al. ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were then transferred to 0.45 µm pore PVDF (catalog number IPVH00010, Millipore) at 25 to 35 V for 1 hour and incubated with appropriate antibodies overnight at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... RNA-YTHDC1-antibody complexes were pulled down using Dynabeads Protein A/G (Millipore) and washed 3 times with NETN100 buffers (20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... Protein alkylation reactions were then carried out with 50 mM Iodoacetamide (IAA) (Sigma) at room temperature for 30 minutes in the dark ...
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bioRxiv - Biophysics 2022Quote: ... Proteins were concentrated using a centrifugal filter unit (3 kDa MWCO, Merck Millipore) at 3500 rpm and 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... The concentrated protein was filtered through a 0.22 μm centrifugal filter (Merck Millipore) prior to loading onto the gel filtration column ...
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bioRxiv - Biochemistry 2022Quote: ... RAF protein was then eluted with 200 μg/mL 3xFLAG peptide (Sigma-Aldrich) in TN buffer ...
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bioRxiv - Cell Biology 2022Quote: Protein concentration was calculated from either Coomassie Brilliant Blue G (Sigma-Aldrich; B0770) staining of SDS-PAGE-separated purified proteins and Bovine Serum Albumin (BSA ...
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bioRxiv - Cell Biology 2022Quote: ... 200 ug protein was loaded onto a Microcon Centrifugal Filters (Millipore, Cat: 42407). Filter aided sample preparation (FASP ...
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bioRxiv - Cell Biology 2022Quote: Total protein was extracted by lysing the cells with RIPA Buffer (Sigma-Aldrich) containing phosphatase and protease inhibitors (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... 350 µg total protein concentrated with 3-kDa cut-off centrifugal filter (Millipore) from serum-free medium was subjected to SDS-PAGE with multiple band excision and in-gel digestion ...
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bioRxiv - Cell Biology 2022Quote: For analysis of oxidized protein abundance using the Oxiblot assay kit (Millipore, S7150), lysates were prepared in RIPA lysis buffer supplemented with 50 μM DTT ...
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bioRxiv - Cell Biology 2022Quote: Chemical cross-linking of proteins was performed with dithio-bismaleimidoethane (DTME) (Sigma-Aldrich) dissolved in dimethyl sulfoxide (DMSO) ...
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bioRxiv - Immunology 2022Quote: ... GFP-tagged SAMHD1 proteins were immunoprecipitated with anti-SAMHD1 antibody (Sigma-Aldrich, SAB1400478) bound to Protein-A/G-Agarose (ThermoFisher ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... proteins were transferred to a 0.2 μm PVDF membrane (#ISEQ85R; Sigma-Aldrich/Merck) by electroblotting the separated proteins for 1 h at 100 V ...
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bioRxiv - Developmental Biology 2022Quote: ... A GST-Drn fusion protein was produced in Origami B (DE3) cells (Novagen) and purified with a glutathione-Sepharose 4B column ...
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bioRxiv - Plant Biology 2022Quote: ... Soluble protein was purified with 200 μl of Glutathione agarose beads slurry (Sigma), gently shaking for 1 hour at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... proteins were transferred to an Immobilon®-P PVDF membrane (Merck Millipore Ltd.) in a Biometra Fastblot B34 chamber at 1 mA/cm2 membrane for 1.5 h in transfer buffer (20 mM CAPSO pH 11.2 ...
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bioRxiv - Plant Biology 2022Quote: ... Protein levels were assessed using immunoblots with HA-HRP (Sigma, Catalog number 12013819001) or FLAG-HRP antibodies (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... 10 µM of indicated proteins were mixed with 100 µM nitrocefin (Calbiochem, Sigma) in a reaction buffer containing EstG storage buffer (50 mM HEPES-NaOH pH 7.2 ...
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bioRxiv - Molecular Biology 2022Quote: These His-tagged protein lysates were combined with 1 ml nickel beads (Sigma) and incubated at 4 °C/2 hours/rolling ...
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bioRxiv - Microbiology 2022Quote: ... Protein fractions were collected from each well and analysed by silver staining (Sigma) and Western blot using monoclonal anti poly histidine alkaline phosphatase antibody (1:20,000 dilution) ...
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bioRxiv - Microbiology 2022Quote: ... was added to 75 μl of prewashed protein A sepharose beads (Sigma-Aldrich) together with 700 μl lysis buffer (20 mM Tris/HCl pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were resolved by SDS-PAGE and transferred onto polyvinylidene difluoride (PVDF; Millipore) membranes ...
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bioRxiv - Microbiology 2022Quote: ... Pure Cas protein was finally concentrated in Amicon Ultra centrifugal filter (Merck-Millipore).
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bioRxiv - Microbiology 2022Quote: ... Digested glycans were cleaned using a 96-well PVDF protein-binding membrane (Millipore) attached to a vacuum manifold ...