Labshake search
Citations for Millipore Sigma :
8801 - 8850 of 10000+ citations for Recombinant Mouse TNFRSF9 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: ... Proteins were reduced with addition of 1 µL of 500 mM dithiothreitol (Sigma) at 25°C for 1 h and alkylated with 3 µL of 500 mM iodoacetamide for 1 h at room temperature in the dark and quenched with 2 µL of dithiothreitol for 10 min ...
-
bioRxiv - Biophysics 2024Quote: ... The dialyzed sample was filtered with a low protein binding filter (Millipore Sigma) and run through a SourceQ15 anion exchange column (Cytiva) ...
-
bioRxiv - Cell Biology 2024Quote: ... FLAG-tagged protein was isolated with anti-FLAG M2 magnetic beads (Sigma, M8823) for 2 hr at 4LJ°C and eluted with 100 μg/ml 3x FLAG peptide ...
-
bioRxiv - Developmental Biology 2023Quote: ... The lysates were pre-cleared using Protein A agarose beads (11134515001, Sigma-Aldrich) at 4 ℃ for 2 h ...
-
bioRxiv - Neuroscience 2024Quote: ... Protein concentrations were determined using the Pierce™ BCA assay kit (Sigma-Aldrich), and equal amounts of protein were analyzed by western blot.
-
bioRxiv - Cell Biology 2024Quote: ... The protein pellet was resuspended in 8M Urea/50mM ammonium bicarbonate (Sigma Aldrich) and centrifuged (12 000 g ...
-
bioRxiv - Molecular Biology 2024Quote: ... Proteins were electrophoretically transferred overnight on to PVDF membrane (03010040001, Roche, Sigma-Aldrich). The membrane was blocked in 5% non-fat dry milk in TBS with 0.05% Tween 20 (TBST ...
-
bioRxiv - Microbiology 2024Quote: ... External mass calibration was performed thanks to SpheriCal Aqua Protein Low kit (Sigma).
-
bioRxiv - Immunology 2024Quote: ... Eluted proteins were buffer exchanged using 50 kDa Amicon centrifugal units (EMD Millipore), resuspended in phosphate-buffered saline (PBS) ...
-
bioRxiv - Molecular Biology 2024Quote: Proteins were transferred from SDS PAGE onto Immobilon-FL PVDF membranes (Millipore Sigma) using a semi-dry transfer apparatus (15V ...
-
bioRxiv - Cell Biology 2024Quote: ... Proteins in the supernatant and pellet were solubilized in 1XLDS sample buffer (Sigma) and analyzed by SDS-PAGE and western blots with anti-CAPS and anti-alpha-tubulin antibodies.
-
bioRxiv - Molecular Biology 2024Quote: ... The collected proteins were concentrated by an Amicon Ultra-3k centrifugal filter (Millipore), exchanged with PBS supplemented with 100 mM NaCl and 50% glycerol ...
-
bioRxiv - Neuroscience 2024Quote: ... proteins were solubilised in solubilising buffer containing 1 M hydroxylamine hydrochloride (159417, Sigma), 1 mM HPDP-biotin (A8008 ...
-
bioRxiv - Neuroscience 2024Quote: ... the proteins were incubated with streptavidin-agarose (16-126; Millipore sigma, Burlington, MA) for one night at 4 °C ...
-
bioRxiv - Microbiology 2024Quote: ... The protein was concentrated using a 10 kDa MWCO Amicon concentrator device (Millipore). The purified proteins and protein standards were subjected to a colorimetric bicinchoninic acid (BCA ...
-
bioRxiv - Cell Biology 2024Quote: ... goat anti-glial fibrillary acidic protein (GFAP) polyclonal antibody (1:1000; Sigma-Aldrich, catalogue# SAB2500462 ...
-
bioRxiv - Developmental Biology 2024Quote: ... FLAG-tagged proteins were purified using anti-FLAG M2 Affinity agarose gel (Sigma) for >1h at 4°C ...
-
bioRxiv - Cell Biology 2024Quote: ... proteins were transferred onto polyvinylidene difluoride (PVDF) membranes (Immobilon; Millipore, Burlington, MA, USA). Incubation with primary antibodies was carried out at 25 °C for 1h ...
-
bioRxiv - Immunology 2024Quote: ... The proteins were transferred from SDS-PAGE to PVDF membrane (0.2-μm, Millipore) in Tris-Glycine Transfer buffer (Bio-Rad ...
-
bioRxiv - Immunology 2024Quote: ... The supernatant was passed through low-protein binding 0.45-µm syringe filter (Millipore). The lentivirus was purified by two different methods for comparison ...
-
bioRxiv - Microbiology 2024Quote: ... The refolded proteins were concentrated with Amicon 3 kDa Ultracel filters (EMD Millipore). For GFP-PSB15 purification ...
-
bioRxiv - Microbiology 2024Quote: ... The protein-containing cell culture supernatant was filtered through 0.22μm PES membranes (Millipore) and passed over 0.5ml of a Strep-Tactin Superflow (IBA Lifesciences ...
-
bioRxiv - Molecular Biology 2023Quote: ... Proteins in the supernatant were digested with 100 µg/mL proteinase K (Sigma) at 37°C overnight ...
-
bioRxiv - Microbiology 2024Quote: ... The total protein concentration in the lysates was quantified using Bradford reagent (Sigma), and equal amounts of bacterial proteins were resolved by SDS-PAGE ...
-
bioRxiv - Microbiology 2024Quote: ... The total protein content of the samples was quantified using Bradford assay (Sigma). Equal concentrations of protein samples were mixed with 5x Laemmle buffer (Bio-Rad) ...
-
bioRxiv - Biochemistry 2024Quote: ... Purified protein samples were concentrated using Amicon Ultra 10K spin column (Sigma-Aldrich) and dialyzed against Storage buffer (25 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Cell Biology 2024Quote: ... protein was reduced (2.5 mM dithiothreitol, Sigma-Aldrich; 30 min at 37°C) and alkylated (10 mM iodacetamide ...
-
bioRxiv - Cancer Biology 2023Quote: ... and 50 µM Rho-associated protein kinase (ROCK) inhibitor (Merck Millipore, SCM-075). On day 3 ...
-
bioRxiv - Cancer Biology 2023Quote: ... the protein was concentrated using an 10K MWCO centrifugal concentrator (Amicon-15, Millipore) to 20 mg/mL and purified by size exclusion chromatography on a Superdex 75 10/300 GL column (GE Healthcare Life Sciences) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Proteins were then precipitated upon addition of 50% trichloroacetic acid (TCA, Sigma-Aldrich) to a final concentration of 5% ...
-
bioRxiv - Molecular Biology 2023Quote: ... Proteins coupled to streptavidin-coated magnetic beads were reduced with dithiothreitol (Sigma-Aldrich) and alkylated with iodoacetamide ...
-
Mitochondrial Apolipoprotein MIC26 is a metabolic rheostat regulating central cellular fuel pathwaysbioRxiv - Cell Biology 2023Quote: ... proteins were transferred onto nitrocellulose membranes and stained using Ponceau S (Sigma Aldrich). After destaining ...
-
bioRxiv - Cell Biology 2023Quote: ... protein was extracted from SC cells using lysis buffer (CelLyticTM M, Sigma-Aldrich) supplemented with Halt™ protease/phosphatase inhibitor cocktail (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2023Quote: ... The immunoprecipitated TFEB proteins were dephosphorylated by λ phosphatase (Cat# P9614, Sigma-Aldrich) following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... aliquots of protein were digested twice (overnight and 4h) with trypsin (Sigma-Aldrich) at 37°C ...
-
bioRxiv - Immunology 2023Quote: ... Total protein concentration was determined using the QuantiPro BCA assay kit (Sigma-Aldrich) by first interpolating the calibration curve using BSA standard solution (0.5 – 30 μg/ml ...
-
bioRxiv - Cancer Biology 2024Quote: ... The lysate was precleared with Protein G agarose beads (EMD Millipore 16-266) for FLAG immunoprecipitation or Protein A agarose beads (EMD Millipore 16-125 ...
-
bioRxiv - Cell Biology 2023Quote: ... proteins from the gel were transferred onto a PVDF membrane (Millipore, cat#IPVH00010). The membrane was subsequently blocked and incubated with primary and secondary antibodies ...
-
bioRxiv - Pathology 2023Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated in the blocking buffer (5% non-fat milk ...
-
bioRxiv - Plant Biology 2023Quote: ... The protein eluent was concentrated using a 100-kDa cut-off Centricon (Millipore) and subsequently loaded onto a Superose 6 Increase 5/150 GL column (Cytiva ...
-
bioRxiv - Cell Biology 2023Quote: ... purified WT or ΔFN2 proteins were incubated with rat tail collagen (EMD Millipore) and then co-IP was carried out as described above.
-
bioRxiv - Physiology 2023Quote: ... Proteins were then concentrated to approximately 3 mg/ml by ultrafiltration (Millipore, UFC905096) at 4 °C ...
-
bioRxiv - Microbiology 2024Quote: ... FLAG-tagged proteins were detected using anti-FLAG M2 primary (Sigma-Aldrich, F1804) antibodies combined with anti-mouse-HRP secondary (Rockland ...
-
bioRxiv - Plant Biology 2024Quote: ... and total protein concentration was measured with Bicinchoninic Acid solution (BCA, Sigma-Aldrich) following manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred onto polyvinylidene fluoride membranes (Millipore, Immobilon PSQ, ISEQ00010 or PVH00010) and the membranes were then blocked for 1 hour in 5% non-fat milk in TBS-T and incubated in primary antibodies overnight in 5% BSA in TBS-T at 4°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted with 5 ml of 1 mg/ml Flag-peptide (Sigma), followed by the addition of 20 ml of QA buffer (50mM TRIS pH 7.7 ...
-
bioRxiv - Microbiology 2023Quote: ... Purified protein was concentrated by centrifugal ultrafiltration using Amicon Ultra 30 K (Millipore) and quantified spectrophotometrically at 280 nm (mass extinction coefficient E 1% ...
-
bioRxiv - Microbiology 2023Quote: ... The proteins were concentrated by centrifugal filters (Amicon Ultra 10K, Merck Millipore Ltd.). Protein concentrations were determined by a Pierce™ BCA Protein Assay Kit (Thermo Fischer Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... proteins were buffer-exchanged against 100 mM ammonium acetate buffer pH 7.4 (Sigma) using Amicon centrifugal filters (Merck ...
-
bioRxiv - Biochemistry 2023Quote: ... Fractions containing protein were concentrated in 50-kDa-cutoff Amicon centrifugal filters (Millipore). Aliquots were frozen in liquid nitrogen and stored at −80 °C.