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Citations for Millipore Sigma :
9201 - 9250 of 10000+ citations for Recombinant Mouse TNFRSF9 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... and total protein concentration was measured with Bicinchoninic Acid solution (BCA, Sigma-Aldrich) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred onto polyvinylidene fluoride membranes (Millipore, Immobilon PSQ, ISEQ00010 or PVH00010) and the membranes were then blocked for 1 hour in 5% non-fat milk in TBS-T and incubated in primary antibodies overnight in 5% BSA in TBS-T at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The separated proteins were then electrotransferred to 0.45 μm PVDF membranes (#IPVH00010, Millipore) at 200 mA for 2 h ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted with 5 ml of 1 mg/ml Flag-peptide (Sigma), followed by the addition of 20 ml of QA buffer (50mM TRIS pH 7.7 ...
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bioRxiv - Microbiology 2023Quote: ... Purified protein was concentrated by centrifugal ultrafiltration using Amicon Ultra 30 K (Millipore) and quantified spectrophotometrically at 280 nm (mass extinction coefficient E 1% ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were concentrated by centrifugal filters (Amicon Ultra 10K, Merck Millipore Ltd.). Protein concentrations were determined by a Pierce™ BCA Protein Assay Kit (Thermo Fischer Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were buffer-exchanged against 100 mM ammonium acetate buffer pH 7.4 (Sigma) using Amicon centrifugal filters (Merck ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions containing protein were concentrated in 50-kDa-cutoff Amicon centrifugal filters (Millipore). Aliquots were frozen in liquid nitrogen and stored at −80 °C.
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bioRxiv - Biochemistry 2023Quote: Proteins in 20 µL solution were precipitated with trichloroacetic acid (TCA; Sigma-Aldrich) at a final concentration of 5% and washed twice with ice-cold acetone ...
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bioRxiv - Biochemistry 2023Quote: ... the proteins were filtered using an Ultrafree 0.22 μm centrifugal filter (Merck Millipore) to remove any aggregation ...
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bioRxiv - Cell Biology 2023Quote: ... the proteins were transferred to 0.45 µm PVDF membranes (Merck Millipore, cat.no. IPVH00010). After blocking unspecific binding sites of the membrane at RT for 1 h with 5 % skim milk powder in TBS-T ...
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bioRxiv - Cell Biology 2023Quote: ... 1% NP-40) supplemented with a protein inhibitor cocktail 1:100 (Sigma-Aldrich). Samples denatured by 3 min boiling in Laemmli buffer were separated by electrophoresis on 4-12% bis-acrylamide gel (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins in samples were detected using Immobilon Western Chemiluminescent HRP Substrate (Sigma-Aldrich) and image acquisition was performed on a G-Box (Ozyme) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein estimation was performed using BCA method as per the manufacturing protocol (Sigma). 20µg of protein from each sample prepared in 1x protein loading dye was used for polyacrylamide gel electrophoresis (Biorad) ...
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bioRxiv - Physiology 2023Quote: ... Protein concentration was determined using a bicinchoninic acid kit (BCA, Sigma, BCA1-1KT) according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Actin protein was detected with anti-Actin antibody (Millipore-Sigma MAB1501, 1:3000). For other immunoblotting ...
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bioRxiv - Pathology 2023Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated in the blocking buffer (5% non-fat milk ...
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bioRxiv - Cell Biology 2023Quote: ... Eluted proteins were concentrated using a 10K Amicon tube (Merck-Millipore, Darmstadt, Germany) and the buffer was changed to PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Eluted proteins were concentrated using a 10K Amicon tube (Merck-Millipore, Darmstadt, Germany) and the buffer was changed to PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 mM EDTA and proteins were degraded using 100 µg proteinase K (Sigma) and incubation for two hours at 55 °C ...
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bioRxiv - Microbiology 2023Quote: ... The purified protein was concentrated to 5 mg/ml by ultrafiltration (Millipore, UFC901096) and maintained in 20 mM Tris-HCl ...
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bioRxiv - Immunology 2023Quote: ... The purified mini-HA protein was concentrated by Amicon spin filter (Millipore Sigma) and filtered by 0.22 µm centrifuge tube filters (Costar) ...
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bioRxiv - Immunology 2023Quote: ... Protein was then transferred onto a 0.45 µm PVDF membrane (Millipore, Cat. IPFL85R), blocked with 5% non-fat dried milk in TBS plus 0.01% Tween (TBST ...
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bioRxiv - Microbiology 2023Quote: ... 50 μl of slurry of Magna ChIP Protein G magnetic beads (Sigma-Aldrich) were washed twice with IP/wash buffer 20 mM Tris HCl pH 7.4 ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins from the gels were transferred onto Immobilon-P polyvinylidene fluoride (PVDF, Millipore) membranes for 90 minutes at 70 V ...
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bioRxiv - Plant Biology 2023Quote: ... Actin protein was detected with anti-Actin antibody (Millipore-Sigma MAB1501, 1:3000). For other immunoblotting ...
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bioRxiv - Neuroscience 2023Quote: ... Chicken anti-Glial Fibrillary Acidic Protein (GFAP) (1:500, Millipore, Cat# AB5541, RRID:AB_177521), Rabbit anti-Homer1 (1:1,000 ...
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bioRxiv - Developmental Biology 2023Quote: ... 40 μl of Protein A Sepharose CL-4B beads (Sigma; GE17-0780-01) was added in the tube and incubated for and additiona1 hour at 4°C under rotation ...
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bioRxiv - Plant Biology 2024Quote: ... HA-tagged proteins were detected by anti-HA Peroxidase high affinity antibody (Sigma). GFP was detected using a horseradish peroxidase-conjugated anti-GFP antibody (1:1.000 ...
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bioRxiv - Immunology 2024Quote: ... 5 mg purified RIPR protein fragment was incubated with TEV protease (Sigma/Promega) at a 1:10 v/v ratio overnight at 4 °C on a rolling mixer ...
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bioRxiv - Immunology 2024Quote: ... or anti-VSV matrix protein antibody (1:5,000 dilution, Sigma-Aldrich, Cat. #MABF2347) and subsequently with HRP-conjugated goat anti-mouse IgG (1:750 dilution ...
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bioRxiv - Plant Biology 2024Quote: ... the proteins were transferred to the polyvinylidene fluoride (PVDF) membrane (0.45 μm, Millipore) and then incubated with GST-14-3-3 ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... and CdtC subunits (within pET-15b protein expression plasmids; #69661, Sigma-Aldrich, USA), each expressed with an amino-terminal polyhistidine fusion peptide ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were detected by immunoblotting with anti-Cdh1 antibodies (1 µg/ml, Millipore) and anti-GST antibodies (0.5 µg/ml ...
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bioRxiv - Microbiology 2024Quote: ... Proteins in the supernatant were precipitated with 1:4 trichloroacetic acid (Sigma Aldrich). The pellet was washed and disrupted with acetone two times and re-dissolved in 200 mM ammonium bicarbonate with 6 M Urea (Sigma Aldrich) ...
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bioRxiv - Biochemistry 2024Quote: ... The eluted protein was concentrated using Amicon Ultra 10K spin columns (Sigma-Aldrich) and dialyzed against hSSB storage buffer.
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bioRxiv - Cancer Biology 2024Quote: ... Lysates were pre-cleared using protein G agarose beads (16-266, Sigma Aldrich). The pre-cleared lysates were incubated with streptavidin-agarose beads (20359 ...
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bioRxiv - Microbiology 2024Quote: ... the proteins were incubated with an equimolar amount of Atto488 maleimide (Sigma-Aldrich) at 4°C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... and FLAG tagged proteins were immunoprecipitated using anti-FLAG M2 affinity gel (Sigma). Elution was carried out with 2X Bolt LDS sample buffer and the samples were blotted onto the PVDF membrane ...
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bioRxiv - Cancer Biology 2024Quote: ... Biotin switch-treated protein reduced with 2.5 μL of 0.2 M dithiothreitol (Sigma) for 1 hr at 57°C and the samples cooled before adding 2.5 μL of 0.5M iodoacetamide and incubated in the dark for 45 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... FLAG-tagged proteins were pulled down using anti-FLAG M2 magnetic beads (Sigma) and serially diluted with enzyme buffer before ATP and fluorescein-conjugated PKC substrate peptide (ThermoFisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... The eluents were then incubated with S-protein agarose (EMD Millipore, 69704-3) overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: Protein lysates were extracted from flash-frozen pellets with RIPA buffer (Sigma R0278), supplemented with protease and phosphatase inhibitors (Invitrogen A32961) ...
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bioRxiv - Plant Biology 2024Quote: ... Protein and nucleotide concentration were determined using the Bradford reagent (Sigma Aldrich, Germany) and HPLC ...
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bioRxiv - Neuroscience 2024Quote: ... Immobilized proteins were eluted by thrombin cleavage (thrombin 0.02 UN/µl, Sigma, Germany). The unbound fraction (Sbh) ...
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bioRxiv - Molecular Biology 2024Quote: ... the protein was re-applied to His-Select Cobalt affinity gel (Sigma-Aldrich). The flow through fractions were pooled and concentrated (Vivaspin Turbo 3,000 MWCO ...
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bioRxiv - Cell Biology 2024Quote: Proteins were resolved in 10% SDS PAGE and transferred to PVDF membrane (Millipore, Burlington ...
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bioRxiv - Immunology 2024Quote: ... The proteins on the gel were then transferred to PVDF membranes (Millipore, IPVH00010), which were blocked in 5% BSA for 1 h at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to a 0.45µm Immobilon-P Membrane (Millipore Sigma cat. IPVH00010) in ice-cold Tris-Glycine Buffer with 20% methanol in a transfer tank either overnight at 10V or 1 hour at 50V ...
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bioRxiv - Molecular Biology 2024Quote: ... Successful protein transfer was confirmed by incubating membranes in Ponceau S solution (Sigma) for 5 minutes with rocking and membranes were blocked in 5% milk in TBS buffer containing 0.1% Tween-20 for 1 hour with gentle rocking ...