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Citations for Millipore Sigma :
8751 - 8800 of 10000+ citations for Recombinant Mouse TNFRSF9 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The concentrated protein was filtered through a 0.22 μm centrifugal filter (Merck Millipore) prior to loading onto the gel filtration column ...
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bioRxiv - Biochemistry 2022Quote: ... RAF protein was then eluted with 200 μg/mL 3xFLAG peptide (Sigma-Aldrich) in TN buffer ...
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bioRxiv - Cell Biology 2022Quote: Protein concentration was calculated from either Coomassie Brilliant Blue G (Sigma-Aldrich; B0770) staining of SDS-PAGE-separated purified proteins and Bovine Serum Albumin (BSA ...
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bioRxiv - Cell Biology 2022Quote: ... 200 ug protein was loaded onto a Microcon Centrifugal Filters (Millipore, Cat: 42407). Filter aided sample preparation (FASP ...
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bioRxiv - Cell Biology 2022Quote: Total protein was extracted by lysing the cells with RIPA Buffer (Sigma-Aldrich) containing phosphatase and protease inhibitors (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... 350 µg total protein concentrated with 3-kDa cut-off centrifugal filter (Millipore) from serum-free medium was subjected to SDS-PAGE with multiple band excision and in-gel digestion ...
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bioRxiv - Cell Biology 2022Quote: For analysis of oxidized protein abundance using the Oxiblot assay kit (Millipore, S7150), lysates were prepared in RIPA lysis buffer supplemented with 50 μM DTT ...
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bioRxiv - Cell Biology 2022Quote: Chemical cross-linking of proteins was performed with dithio-bismaleimidoethane (DTME) (Sigma-Aldrich) dissolved in dimethyl sulfoxide (DMSO) ...
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bioRxiv - Immunology 2022Quote: ... GFP-tagged SAMHD1 proteins were immunoprecipitated with anti-SAMHD1 antibody (Sigma-Aldrich, SAB1400478) bound to Protein-A/G-Agarose (ThermoFisher ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... proteins were transferred to a 0.2 μm PVDF membrane (#ISEQ85R; Sigma-Aldrich/Merck) by electroblotting the separated proteins for 1 h at 100 V ...
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bioRxiv - Developmental Biology 2022Quote: ... A GST-Drn fusion protein was produced in Origami B (DE3) cells (Novagen) and purified with a glutathione-Sepharose 4B column ...
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bioRxiv - Plant Biology 2022Quote: ... Soluble protein was purified with 200 μl of Glutathione agarose beads slurry (Sigma), gently shaking for 1 hour at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... proteins were transferred to an Immobilon®-P PVDF membrane (Merck Millipore Ltd.) in a Biometra Fastblot B34 chamber at 1 mA/cm2 membrane for 1.5 h in transfer buffer (20 mM CAPSO pH 11.2 ...
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bioRxiv - Plant Biology 2022Quote: ... Protein levels were assessed using immunoblots with HA-HRP (Sigma, Catalog number 12013819001) or FLAG-HRP antibodies (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... 10 µM of indicated proteins were mixed with 100 µM nitrocefin (Calbiochem, Sigma) in a reaction buffer containing EstG storage buffer (50 mM HEPES-NaOH pH 7.2 ...
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bioRxiv - Molecular Biology 2022Quote: These His-tagged protein lysates were combined with 1 ml nickel beads (Sigma) and incubated at 4 °C/2 hours/rolling ...
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bioRxiv - Microbiology 2022Quote: ... Protein fractions were collected from each well and analysed by silver staining (Sigma) and Western blot using monoclonal anti poly histidine alkaline phosphatase antibody (1:20,000 dilution) ...
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bioRxiv - Microbiology 2022Quote: ... was added to 75 μl of prewashed protein A sepharose beads (Sigma-Aldrich) together with 700 μl lysis buffer (20 mM Tris/HCl pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were resolved by SDS-PAGE and transferred onto polyvinylidene difluoride (PVDF; Millipore) membranes ...
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bioRxiv - Microbiology 2022Quote: ... Pure Cas protein was finally concentrated in Amicon Ultra centrifugal filter (Merck-Millipore).
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bioRxiv - Microbiology 2022Quote: ... Digested glycans were cleaned using a 96-well PVDF protein-binding membrane (Millipore) attached to a vacuum manifold ...
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bioRxiv - Molecular Biology 2022Quote: ... Pellets were transferred to low protein binding tubes (Sigma-Aldrich, Cat# Z666513-100EA) and stored at −80°C
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bioRxiv - Neuroscience 2022Quote: ... and the separated proteins were then transferred to polyvinylidene difluoride membranes (Millipore, USA). These membranes were blocked in 5% (w/v ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany). Unspecific binding on the PVDF membrane was blocked by incubation with the blocking buffer (5% non-fat milk ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were resuspended in 50 mM triethylammonium bicarbonate (TEAB, Sigma cat. no. T7408), reduced for 1 hour at 55°C using 10 mM tris(2-carboxyethyl)phosphine (TCEP ...
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bioRxiv - Neuroscience 2023Quote: ... Residual protein fragments were precipitated with trichloroacetic acid (Sigma-Aldrich, cat. no. T6399). The supernatant ...
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bioRxiv - Microbiology 2022Quote: ... YifLStrep protein was eluted with wash buffer containing 2.5 mM d-Desthiobiotin (Sigma).
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bioRxiv - Neuroscience 2022Quote: ... The expression of neuronal marker protein NeuN (anti-NeuN, 1:200, MAB377, Millipore) was determined to identify neuronal tdTomato expression.
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bioRxiv - Neuroscience 2022Quote: ... Protein concentrations were estimated using a Quantipro BCA assay kit (Sigma-Aldrich, USA). Protein lysates were prepared in a mixture of RIPA and Lamelli Buffer to produce a final protein concentration of 5 ug/ul ...
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bioRxiv - Molecular Biology 2022Quote: ... The resolved protein samples were transferred onto Immobilon-P PVDF Membrane (Millipore Sigma), and the blots incubated in the presence of primary antibodies (provided in table S1 ...
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bioRxiv - Molecular Biology 2022Quote: ... Equal loading of protein samples was confirmed via Ponceau S staining (Sigma-Aldrich) for 4 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Cleaved proteins were filtered by centrifuging with Ultrafree-MC VV filter (EMD Millipore) in a tabletop centrifuge at 4 °C ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... Proteins were analyzed by SDS-PAGE and transferred onto PVDF membrane (Merck Millipore). The membranes were washed with PBS 1x containing 0.1% Tween-20 (PBS-T ...
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bioRxiv - Cell Biology 2022Quote: ... Eluted protein fractions were pooled and concentrated using Amicon 30K Centrifugal filters (Millipore). The concentrated ...
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bioRxiv - Cell Biology 2022Quote: ... Elution fractions containing protein were concentrated using Amicon Ultracel-10K/ Ultracel-30K (Millipore) centrifuge filters and subsequently applied to size exclusion chromatography (SEC ...
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bioRxiv - Neuroscience 2022Quote: ... 10 μg protein was combined with 1 μL 10% Triton X-100 (Sigma) and brought to a volume of 33 μL in Vance buffer (225 mM D-mannitol ...
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bioRxiv - Bioengineering 2022Quote: ... 1 mM purified protein was mixed with 25% ammonium persulfate (APS) (Sigma-Aldrich) and 6.67 mM [Ru(II)(bpy)3]2+ (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... The expressed GST-fusion protein was absorbed to glutathione-Sepharose 4B (Sigma-Aldrich) followed by extensive washing with phosphate buffered saline (PBS ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins in the supernatant and pellet were solubilized in 1XLDS sample buffer (Sigma) and analyzed by SDS-PAGE and western blots with anti-CAPS and anti-alpha-tubulin antibodies.
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bioRxiv - Neuroscience 2024Quote: ... Protein concentrations were determined using the Pierce™ BCA assay kit (Sigma-Aldrich), and equal amounts of protein were analyzed by western blot.
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bioRxiv - Microbiology 2024Quote: ... FLAG-tagged proteins were detected using anti-FLAG M2 primary (Sigma-Aldrich, F1804) antibodies combined with anti-mouse-HRP secondary (Rockland ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred onto polyvinylidene fluoride membranes (Millipore, Immobilon PSQ, ISEQ00010 or PVH00010) and the membranes were then blocked for 1 hour in 5% non-fat milk in TBS-T and incubated in primary antibodies overnight in 5% BSA in TBS-T at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... HA-tagged proteins were detected by anti-HA Peroxidase high affinity antibody (Sigma). GFP was detected using a horseradish peroxidase-conjugated anti-GFP antibody (1:1.000 ...
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bioRxiv - Cell Biology 2024Quote: ... The protein pellet was resuspended in 8M Urea/50mM ammonium bicarbonate (Sigma Aldrich) and centrifuged (12 000 g ...
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bioRxiv - Immunology 2024Quote: ... or anti-VSV matrix protein antibody (1:5,000 dilution, Sigma-Aldrich, Cat. #MABF2347) and subsequently with HRP-conjugated goat anti-mouse IgG (1:750 dilution ...
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bioRxiv - Immunology 2024Quote: ... 5 mg purified RIPR protein fragment was incubated with TEV protease (Sigma/Promega) at a 1:10 v/v ratio overnight at 4 °C on a rolling mixer ...
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bioRxiv - Plant Biology 2024Quote: ... and total protein concentration was measured with Bicinchoninic Acid solution (BCA, Sigma-Aldrich) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Eluted proteins were concentrated using a 10K Amicon tube (Merck-Millipore, Darmstadt, Germany) and the buffer was changed to PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Eluted proteins were concentrated using a 10K Amicon tube (Merck-Millipore, Darmstadt, Germany) and the buffer was changed to PBS ...
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bioRxiv - Microbiology 2023Quote: ... The purified protein was concentrated to 5 mg/ml by ultrafiltration (Millipore, UFC901096) and maintained in 20 mM Tris-HCl ...