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Citations for Millipore Sigma :
9101 - 9150 of 10000+ citations for Recombinant Mouse TNFRSF9 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... the proteins were incubated with streptavidin-agarose (16-126; Millipore sigma, Burlington, MA) for one night at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... Eluted proteins were buffer exchanged using 50 kDa Amicon centrifugal units (EMD Millipore), resuspended in phosphate-buffered saline (PBS) ...
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bioRxiv - Molecular Biology 2024Quote: ... The separated proteins were then electrotransferred to 0.45 μm PVDF membranes (#IPVH00010, Millipore) at 200 mA for 2 h ...
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bioRxiv - Microbiology 2024Quote: ... The protein-containing cell culture supernatant was filtered through 0.22μm PES membranes (Millipore) and passed over 0.5ml of a Strep-Tactin Superflow (IBA Lifesciences ...
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bioRxiv - Microbiology 2023Quote: ... G9R and A1L proteins were labeled with primary anti-Flag (F1804, Sigma-Aldrich) and anti-HA (26183 ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by protein detection using either Immobilon Western HRP Substrate (WBKLS0500, Geyer/Millipore) or SuperSignal West Femto Maximum Sensitivity Substrate (34095 ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were transferred onto polyvinylidene difluoride (PVDF) membranes (Immobilon; Millipore, Burlington, MA, USA). Incubation with primary antibodies was carried out at 25 °C for 1h ...
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bioRxiv - Plant Biology 2023Quote: ... Actin protein was detected with anti-Actin antibody (Millipore-Sigma MAB1501, 1:3000). For other immunoblotting ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins from the gels were transferred onto Immobilon-P polyvinylidene fluoride (PVDF, Millipore) membranes for 90 minutes at 70 V ...
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bioRxiv - Cancer Biology 2024Quote: ... The lysate was precleared with Protein G agarose beads (EMD Millipore 16-266) for FLAG immunoprecipitation or Protein A agarose beads (EMD Millipore 16-125 ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins in the supernatant were digested with 100 µg/mL proteinase K (Sigma) at 37°C overnight ...
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bioRxiv - Microbiology 2024Quote: ... The total protein concentration in the lysates was quantified using Bradford reagent (Sigma), and equal amounts of bacterial proteins were resolved by SDS-PAGE ...
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bioRxiv - Microbiology 2024Quote: ... The total protein content of the samples was quantified using Bradford assay (Sigma). Equal concentrations of protein samples were mixed with 5x Laemmle buffer (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins of interest were detected with anti-FLAG antibody (Sigma F1805, 1:1000) and anti-PCNA antibody (Santa Cruz sc-8349 ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were reduced with addition of 1 µL of 500 mM dithiothreitol (Sigma) at 25°C for 1 h and alkylated with 3 µL of 500 mM iodoacetamide for 1 h at room temperature in the dark and quenched with 2 µL of dithiothreitol for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... protein was reduced (2.5 mM dithiothreitol, Sigma-Aldrich; 30 min at 37°C) and alkylated (10 mM iodacetamide ...
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bioRxiv - Biochemistry 2024Quote: ... Purified protein samples were concentrated using Amicon Ultra 10K spin column (Sigma-Aldrich) and dialyzed against Storage buffer (25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2024Quote: ... goat anti-glial fibrillary acidic protein (GFAP) polyclonal antibody (1:1000; Sigma-Aldrich, catalogue# SAB2500462 ...
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bioRxiv - Developmental Biology 2024Quote: ... FLAG-tagged proteins were purified using anti-FLAG M2 Affinity agarose gel (Sigma) for >1h at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Purified proteins were concentrated using Amicon™ Ultra centrifugal filter units (Millipore Sigma) with a molecular cutoff of 10 kDa and stored at −80 °C.
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bioRxiv - Microbiology 2024Quote: ... The refolded proteins were concentrated with Amicon 3 kDa Ultracel filters (EMD Millipore). For GFP-PSB15 purification ...
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bioRxiv - Immunology 2024Quote: ... The proteins were transferred from SDS-PAGE to PVDF membrane (0.2-μm, Millipore) in Tris-Glycine Transfer buffer (Bio-Rad ...
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bioRxiv - Immunology 2024Quote: ... The supernatant was passed through low-protein binding 0.45-µm syringe filter (Millipore). The lentivirus was purified by two different methods for comparison ...
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bioRxiv - Biochemistry 2023Quote: Proteins in 20 µL solution were precipitated with trichloroacetic acid (TCA; Sigma-Aldrich) at a final concentration of 5% and washed twice with ice-cold acetone ...
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bioRxiv - Biochemistry 2023Quote: ... The cell lysate was pre-cleared with Protein A agarose beads (Millipore, Sigma), and 700 μg of the pre-cleared lysate was incubated with 2 μg of either anti-CEP41 ...
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bioRxiv - Biochemistry 2024Quote: ... Protein samples were incubated with 0.2mM methoxypolyethyleneglycol maleimide (NEM-PEG, 5kDa; Sigma-Aldrich) for 1 minute on ice ...
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bioRxiv - Biochemistry 2024Quote: ... Immunoreactive proteins were visualized using Immobilon® Forte Western HRP Substrate (Merck Millipore) at the Imager Fluor-Chem M (Proteinsimple) ...
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bioRxiv - Biochemistry 2024Quote: ... the protein was concentrated in a centrifugal filter (Amicon Ultra-15, 10K, Millipore) and washed twice with 15 ml of PBS to remove the imidazole ...
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bioRxiv - Immunology 2024Quote: ... the proteins were transferred to polyvinylidene fluoride membrane (EMD Millipore, Billerica, MA, USA). The PVDF membranes were then incubated with a buffer of 5% nonfat dry milk for 2 hours at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... The cell lysate was pre-cleared with Protein A agarose beads (Millipore, Sigma), and 700 μg of the pre-cleared lysate was incubated with 2 μg of either anti-CEP41 ...
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bioRxiv - Biophysics 2024Quote: ... The dialyzed sample was filtered with a low protein binding filter (Millipore Sigma) and run through a SourceQ15 anion exchange column (Cytiva) ...
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bioRxiv - Cell Biology 2024Quote: ... FLAG-tagged protein was isolated with anti-FLAG M2 magnetic beads (Sigma, M8823) for 2 hr at 4LJ°C and eluted with 100 μg/ml 3x FLAG peptide ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-Purkinje cell protein 4 (PCP4, 1:500, Sigma-Aldrich, Cat# HPA005792), rabbit anti-zinc transporter 3 (ZnT3 ...
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bioRxiv - Neuroscience 2023Quote: ... Chicken anti-Glial Fibrillary Acidic Protein (GFAP) (1:500, Millipore, Cat# AB5541, RRID:AB_177521), Rabbit anti-Homer1 (1:1,000 ...
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bioRxiv - Developmental Biology 2023Quote: ... and separated proteins were then transferred to PVDF membranes (polyvinylidene difluoride, Merck Millipore). After treatments with a blocking reagent (Blocking One solution ...
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bioRxiv - Microbiology 2023Quote: ... Secreted proteins were precipitated by addition of 10 % trichloroacetic acid (TCA, Sigma-Aldrich) followed by centrifugation for 1 h at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Flag-tagged proteins were bound to anti-FLAG M2 affinity gel (Sigma-Aldrich) for 1 h at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... The proteins were eluted and concentrated with Amicon Centrifugal Filter Units 10K (Millipore) to remove excessive glutathione.
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bioRxiv - Cancer Biology 2023Quote: ... samples were incubated with biotin-conjugated hyaluronic acid binding protein (HABP) (Sigma 385911), followed by streptavidin conjugated to AlexaFluor-488 (ThermoFisher S11223) ...
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bioRxiv - Pathology 2023Quote: AAA tissue protein was extracted using RIPA buffer with proteinase inhibitor (Sigma #MCL1). Protein quantification was done by Bradford assay ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins on the gel were transferred to a PVDF membrane (Millipore IPFL 00010), which was first blocked with 10 % milk ...
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bioRxiv - Bioengineering 2022Quote: ... Protein bands were visualized by ECL detection kit (EMD Millipore, Burlington, MA, USA) and images were acquired using the ChemiDoc MP image system (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: Proteins were precipitated from sucrose fractions using trichloroacetic acid (TCA; Sigma-Aldrich, T6399). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... Eluates were concentrated with Microcon PL-30 (30,000 NMWL) protein concentrators (Millipore Sigma) and loaded onto 1 cm diameter ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were then transferred onto hydrophobic polyvinylidene difluoride membranes (PVDF, 0.45 µm, Millipore), followed by blocking in milk buffer (Tris-buffered saline [TBS] [0.5 M Tris pH 8.4 ...
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bioRxiv - Microbiology 2023Quote: ... Pre-prepared Protein A Dynabeads (incubated with 5 μg of S9.6 antibody (Millipore) in 1X PBS plus 0.5% BSA overnight at 4°C) ...
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bioRxiv - Cell Biology 2023Quote: ... Cell proteins were isolated by the SDS-PAGE blotted on PVDF membranes (Millipore). And then blocked with 5% fat-free dry ilk at RT for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... after which the interesting proteins were competitively eluted using 3XFLAG peptide (Sigma, F4799). The eluent was then incubated overnight at 4°C with Ni-NTA beads (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: ... Protein concentration in supernatants was analyzed with the Bradford reagent (Sigma-Aldrich, USA) using bovine serum albumin in lysis buffer as a standard solution ...
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bioRxiv - Neuroscience 2023Quote: ... the proteins were expressed in Escherichia coli strain BL21(DE3) (Novagen, Darmstadt, Germany) and cells were lysed with a cell disruptor (Avestin ...