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Citations for Millipore Sigma :
8451 - 8500 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... and then incubated in 8 mL of RPMI assay medium containing the desired glucose concentration (0 – 11 mM glucose) (Sigma Aldrich), supplemented with 10% dialyzed FBS (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: Hemolymph from 3rd instar larvae was extracted as described previously 54 and the circulating glucose and trehalose were measured with the Glucose HK assay reagent (Sigma; GAHKLJ20) and trehalase from porcine kidney (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... Flow chambers were then washed with motility assay buffer supplemented with 1 mg/mL casein and K560-GFP added in the final imaging buffer (motility assay buffer supplemented with an oxygen scavenger system of 45 μg mL−1 glucose catalase (Sigma-Aldrich), and 1.15 mg mL−1 glucose oxidase (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... After 72 hrs we performed 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assays using the Roche MTT protocol (Sigma Aldrich Cat#11465007001). After 24 hours light absorption at 570 nm was measured using the MTT plate reader (Wallac EnVision 1.12) ...
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bioRxiv - Neuroscience 2020Quote: ... The TUNEL protocol was performed using a commercial kit (ApopTag® fluorescein in situ apoptosis detection kit, Millipore). Apoptotic cells were detected as localized bright green cells in a blue background (DAPI ...
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bioRxiv - Neuroscience 2024Quote: ... and some of the myelin was labelled with the green-fluorescent cell linker kit (PKH67 labeling kit, Sigma), according to manufacturer’s specifications.
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bioRxiv - Systems Biology 2023Quote: Leptin - Leptin concentrations were determined by radioimmunoassay using a kit from EMD Millipore (Burlington, MA, kit HL-81K).
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bioRxiv - Molecular Biology 2024Quote: ... we used commercially available ELISA kits: hs-CRP Human C-Reactive Protein ELISA Kit (#RAB0096, Sigma-Aldrich, Germany) and Mercodia Insulin ELISA (#10-1113-01 ...
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bioRxiv - Developmental Biology 2021Quote: ... The lungs and metanephric kidneys were dissected in ice-cold PBS and cultured at the liquid-air interface (Grobstein, 1956) using a trans-well membrane culture system supplemented with explant culture medium (DMEM/F12, 10% FBS (Sigma-Aldrich Chemie GmbH; F9665-500ML), 1x GlutaMAX (Life Technologies Europe BV ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed with Tris-buffered saline/Tween 20 and then incubated with an anti-acetyl-α-tubulin (SIGMA-ALDRICH #T7451 Clone 6-11B-1), α-tubulin (SIGMA-ALDRICH #T9026) ...
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bioRxiv - Bioengineering 2021Quote: Unbound DNA was removed from DNA-SWCNT samples prepared by direct sonication and MeOH-assisted surfactant exchange by rinsing with Amicon centrifugal ultra-filtration devices (Amicon Ultra-2, Sigma Aldrich, 100 kDa membrane, Merck), as detailed above ...
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bioRxiv - Microbiology 2022Quote: ... and pooled in equimolar amounts before concentration using an Amicon® Ultra 0.5 mL centrifugal filter with Ultracel-30K membrane (Millipore Sigma, Billerica, MA, United States) to a final volume of 80 μL ...
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bioRxiv - Cancer Biology 2022Quote: Western blot analysis was done on a 4-12% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) gel and transferred to polyvinylidene difluoride (PVDF) membranes (Millipore-Sigma, Immobiolon-P, Cat # IPVH20200) as previously described [1] ...
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bioRxiv - Microbiology 2020Quote: ... 100,000 x g (membrane fraction) centrifugations were suspended in 100 μL of 2X loading buffer (4% SDS, 10% β-mercaptoethanol, Sigma-Aldrich, 20% (v/v) glycerol ...
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bioRxiv - Molecular Biology 2020Quote: ... the membrane was subsequentially incubated with a horseradish peroxidase (HRP)-conjugated α-mouse whole IgG antibody produced in rabbit (Sigma-Aldrich, CAT. #A9044; 1:80000). Protein-HRP chemiluminescence was detected using Chemidoc XRS1 System (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... were boiled in SDS-PAGE loading buffer and subjected to SDS-PAGE and subsequently transferred to a polyvinylidene fluoride membrane (Millipore-Sigma, St. Louis, MO, USA) using a semidry transfer apparatus (Bio-Rad ...
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bioRxiv - Biochemistry 2022Quote: ... Stock solutions were sterilized using a sterile syringe filter (hydrophilic polyvinylidene difluoride membrane, 0.22 μm pore size, Sigma-Aldrich Chemicals Company, St. Louis, MO, USA) and stored at −20 □C.
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bioRxiv - Cancer Biology 2023Quote: ... a 40 μg aliquot of protein from each extract was separated by Tris-glycine sodium dodecyl-sulfate polyacrylamide gel electrophoresis (4% to 20%) and the protein bands were transferred onto polyvinylidene-fluoride membranes (Sigma-Aldrich, St. Louis, MO, USA), which were subsequently blocked with non-fat milk ...
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bioRxiv - Bioengineering 2023Quote: ... loaded to a polyacrylamide gel (30 μg of total protein/lane) and after gel electrophoresis and protein transfer to polyvinylidene difluoride (PVDF) membranes (Millipore, cat. no. IPVH00010, Burlington, MA). The membranes were blocked with 5% milk in Tris-buffered saline with 0.1% Tween-20 (TBST ...
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bioRxiv - Immunology 2022Quote: ... membranes were incubated with PBS-T or TBS-T complemented with 5 % BSA and primary antibodies specific for β-actin (Sigma-Aldrich, A5441, 1:2000), calnexin (Enzo Life science ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell lysates were run in either 7.5% or 12% SDS-PAGE gel and then transferred into a PVDF Immobilon-P 0.45 mm membrane (Millipore Sigma, Saint Louis, MO, USA, cat# IPVH00010). Membranes were blocked in 5% nonfat dry milk and then incubated in primary antibodies overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... quenched and 6-Carboxyfluorescein-filled liposomes were extruded through Whatman® Nuclepore™ Track-Etched Membrane (pore size=200 nm) (Sigma Aldrich, Saint Louis, MO) as previously described(Jimah et al. ...
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bioRxiv - Microbiology 2022Quote: ... The obtained denatured protein (30 μg) was separated by 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and blotted to a PVDF membrane (Millipore®, St Louis, MO, USA). After blocking non-specific protein with 2% bovine serum albumin ...
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bioRxiv - Neuroscience 2024Quote: ... The membranes were carefully removed from the Bio-Dot® SF Apparatus and stained with ATX Ponceau S red solution (Sigma-Aldrich, 09189-1L-F) for total protein quantification ...
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bioRxiv - Immunology 2021Quote: ... Gastrin EIA kit was purchased from Sigma-Millipore ...
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bioRxiv - Immunology 2021Quote: ... by using Gastrin EIA kit from Millipore-Sigma.
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bioRxiv - Developmental Biology 2020Quote: ... and processed according to Kit 91A (Sigma) protocol for monocyte detection ...
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bioRxiv - Bioengineering 2022Quote: ... Hydrogels (Sigma-Aldrich, TrueGel3D Hydrogel Kit, TRUE7) of ∼10kPa were prepared by thawing of the SLO-DEXTRAN solution ...
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bioRxiv - Cancer Biology 2021Quote: ... The Cell Proliferation Kit II XTT (Sigma) was used according to the manufacturer ...
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bioRxiv - Cancer Biology 2020Quote: ... with a customized Milliplex kit (Merck Millipore).
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bioRxiv - Microbiology 2021Quote: ... following manufacturer’s instructions (Alkaline phosphatase Kit, Sigma). The intensity of pink stain reflects the amount of alkaline phosphatase in positive cells.
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bioRxiv - Neuroscience 2020Quote: ... The KAPA Taq PCR kit (Sigma, BK1000) was used in conjunction with three primer sequences (wild type ...
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bioRxiv - Cell Biology 2022Quote: ... were detected by ELISA Kits (from Millipore) within 24h according to manufacturer instructions ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-vimentin (kit # 17-10195, Millipore), goat polyclonal anti-rabbit-HRP (Biorad ...
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bioRxiv - Cancer Biology 2021Quote: ... The Duolink PLA kit (DUO92007, Sigma-Aldrich) was used according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Leukocyte (TRAP) Kit (Sigma-Aldrich, Burlington, US) at 37°C ...
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bioRxiv - Immunology 2022Quote: ... or Enzymatic Protein Biotinylation Kit (Sigma-Aldrich). SARS-CoV-2 RDB protein was also coupled to DyLight 650 using the DyLight® Amine-Reactive Dyes kit (Thermo Fisher scientific).
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bioRxiv - Neuroscience 2022Quote: ... The ApopTag TUNEL stain kit (Millipore #S7165) was used for Figures 3B ...
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bioRxiv - Physiology 2020Quote: ... Serum triglyceride determination (TG) kit (Sigma, USA) and free fatty acid assay (FFA ...
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bioRxiv - Molecular Biology 2021Quote: ... KAPAR SYBR Fast Universal kit (Sigma KK4602)
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bioRxiv - Genetics 2020Quote: ... or GenElute plasmid mini kit (Sigma-Aldrich). When the latter was used ...
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bioRxiv - Genetics 2020Quote: ... the Imprint DNA Modification Kit (Sigma Aldrich) was used for bisulfite treatment on 200ng DNA ...
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bioRxiv - Synthetic Biology 2021Quote: ... or GenEluteTM Plasmid MiniPrep Kit (Sigma, PLN70). Sequence of every cloned plasmid was verified using Sanger Sequencing performed at the Centre for Applied Genomics at the Hospital for Sick Children ...
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bioRxiv - Cell Biology 2021Quote: ... we used Cholecystokinin EIA Kit (Sigma-Aldrich), following manufacturer’s instructions
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bioRxiv - Microbiology 2021Quote: ... Protein refolding kit was purchased from Novagen. Proteins in pellet (inclusion body ...
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bioRxiv - Microbiology 2020Quote: ... Milliplex MAP Kit (Millipore, Billerica, MA, USA). Twelve cytokines/chemokines ...
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bioRxiv - Molecular Biology 2020Quote: ... following the manufacturer’s protocol (ECL Kit, Millipore), and digital images were acquired using ChemiDoc XRS+System (BioRad) ...
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bioRxiv - Genomics 2021Quote: ... and Glass Dounce Kit (Sigma Aldrich D8938) as previously described2 ...
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bioRxiv - Synthetic Biology 2023Quote: Spectrum Plant Total RNA Kit (Sigma-Aldrich) was used for extracting RNA from ripe berries of V ...
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bioRxiv - Microbiology 2023Quote: ... the CelLytic MEM protein extraction kit (Sigma) was used as described elsewhere (López & Kolter ...