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Citations for Millipore Sigma :
8651 - 8700 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... Using the Spectrum Plant Total RNA kit (Sigma), we isolated RNA from each sample according to the manufacturer’s protocol and performed On-Column DNAse I digestion (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... The LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) was used to assure that all cell lines were mycoplasma-free ...
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bioRxiv - Plant Biology 2024Quote: ... we obtained all chemicals and kits from Sigma–Aldrich Inc.
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bioRxiv - Cancer Biology 2024Quote: ... or liver dECM hydrogel kit (CC174, Sigma-Aldrich), according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... which used the DUOlink PLA kit (Sigma Aldrich) according to manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... or GenEluteTM HP Plasmid Midiprep kit (Sigma-Aldrich) or PureLinkTM HiPure Plasmid filter Maxiprep Kit (Thermo Scientific ...
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bioRxiv - Microbiology 2024Quote: The bacterial genomic extraction kit (Sigma, Darmstadt, Germany) was used to extract the genomic DNA of MKAK9 ...
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bioRxiv - Molecular Biology 2024Quote: ... and an ECL kit (Millipore Corporation, Billerica, MA). Membranes were scanned on a Sapphire Biomolecular Imager (Azure Biosystem ...
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bioRxiv - Plant Biology 2024Quote: Spectrum™ Plant Total RNA Kit (Sigma-Aldrich) was used to isolate the RNA with on a column DNA digestion step according to the manufacturer’s protocol (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2024Quote: ... After plasmid extraction (Miniprep kit, Sigma-Aldrich/Merck), plasmids were verified by Sanger sequencing at Microsynth.
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bioRxiv - Biochemistry 2024Quote: The Citrate Synthase Kit (Sigma-Aldrich Cat. CS0720) was adapted for use in this assay ...
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bioRxiv - Zoology 2024Quote: ... solution or Fast Red Kit (Sigma-Aldrich, USA) in the dark and the reaction was stopped when desired intensity of signal was achieved ...
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bioRxiv - Bioengineering 2024Quote: ... Human IL-8 ELISA Kit (RAB0319, Sigma Aldrich), Human TNF Alpha ELISA Kit (ab181421 ...
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bioRxiv - Bioengineering 2024Quote: ... Human IL-6 ELISA Kit (RAB0306, Sigma Aldrich), Human IL-8 ELISA Kit (RAB0319 ...
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bioRxiv - Cancer Biology 2024Quote: ... (EF5 Hypoxia Detection Kit, Sigma-Aldrich, EF5-30A4). As second antibodies ...
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bioRxiv - Bioengineering 2024Quote: ... cell counting kit-8 (CCK-8, Sigma-Aldrich) was used to evaluate the proliferation of NIH/3T3 over a time course ...
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bioRxiv - Cancer Biology 2024Quote: ... Duo-link kit protocol was used (Sigma Aldrich). JMJD6 and YBX1 primary antibodies were used at suitable dilutions and PCR and secondary probing was done using reagents provided in the kit ...
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bioRxiv - Cancer Biology 2024Quote: ... probes and green detection reagent kit (Sigma, DUO92014). The following antibodies and concentrations were utilized ...
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bioRxiv - Bioengineering 2024Quote: ... kit (#11746081001, Millipore Sigma-Aldrich, St. Louis, Missouri). Reagents I and II were used as provided and not diluted ...
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bioRxiv - Plant Biology 2020Quote: 15N-accumulation assay after 15N-nitrate or 15N-ammonium labeling was performed with 15N-labeled KNO3 (99 atom % 15N, Sigma-Aldrich, no. 335134) or 15N-labeled NH4Cl (98 atom % 15N ...
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bioRxiv - Microbiology 2021Quote: ... Alginate lyase was removed from culture medium prior to assay by filtering the medium through 3 kDa Amicon Ultra molecular weight cutoff filters (MilliPore Sigma, Burlington, MA). To maintain plasmids in 12B01,12.5 µg/mL chloramphenicol was added to the medium.
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bioRxiv - Microbiology 2022Quote: ... The major components of the assay included: 1) rS-RBD coating of plates as target antigen (2ug/ml in Sigma Carbonate-Bicarbonate Buffer); 2 ...
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bioRxiv - Biochemistry 2020Quote: ... Components for solubilisation buffer and ligands for both ThermoFRET stability assay and fluorescent ligand binding experiments were obtained from Sigma-Aldrich (Havervill, UK) and Anatrace (Ohio ...
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bioRxiv - Biochemistry 2020Quote: ... The GnT I assay was subsequently performed in GnT I activity buffer [63] containing 5 mM of UDP-GlcNAc (Sigma-Aldrich Chemie GmbH).
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bioRxiv - Cancer Biology 2021Quote: Cytotoxicity of USP7 inhibitor P5091 and P22077 was determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay (Sigma-Aldrich, St. Louis, MO, USA). Cytotoxicity was assessed after 24 hrs and 48 hrs of inhibitor treatment in 96 well plates.
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bioRxiv - Microbiology 2020Quote: The MILLIPLEX MAP Non-Human Primate Cytokine Magnetic Bead Panel - Premixed 23 Plex – Immunology Multiplex Assay (Millipore, Burlington, MA, USA; #PCYTMG-40K-PX23) was performed on collected plasma samples following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... A portion of the frozen somatosensory cortex\tissue was homogenized in radioimmunoprecipitation assay (RIPA) buffer with protease and phosphatase inhibitors (SigmaFast, S8820, Sigma-Aldrich, USA and PhosStop, 4906837001, Sigma-Aldrich, USA) The protein concentration of the lysates was quantified using a BCA Protein Assay Kit (Thermo Scientific Pierce Biotechnology ...
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bioRxiv - Neuroscience 2021Quote: ... Milliplex magnetic bead panel assays (CCL2, CXCL10, IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-6, and IL-10; Millipore Sigma, Burlington, MA) were used as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... or only on the bottom side (for tumor haptotaxis assays) of 8 or 3 μm hanging cell culture inserts (Millipore, MCEP24H48 and MCSP24H48). After washing the filters with PBS ...
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bioRxiv - Pathology 2021Quote: ... ALT was measured in 1 microliter of plasma collected via cardiac puncture or tail vein bleed using a colorimetric assay (Millipore Sigma cat. # MAK052) according to the manufacturer’s instructions.
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Potential for virus endogenization in humans through testicular germ cell infection: the case of HIVbioRxiv - Microbiology 2020Quote: ... Neutralization assays were performed by pre-incubating cells with anti-GalactosylCeramide antibody (mGalC, 10µg/mL, Millipore 10µg/mL, 30 min at 4°C) or viral inoculum with monoclonal antibody against HIV-1 gp120 (1-2µg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated for 24h before monitoring cell viability using the MTT assay: cells were incubated at 35°C for 1 h with MTT (Sigma, Cat #M2128-10G). For that purpose ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantification of siderophores produced by AMB-1 was performed using the Chrome Azurol S (CAS) assay (199532, Sigma-Aldrich, St. Louis, Missouri, USA) [47] ...
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bioRxiv - Microbiology 2022Quote: ... IgG assay samples were incubated with 100 μL of pre-washed 50/50 Protein Sepharose A slurry (Sigma-Aldrich, diluted in lysis buffer) for 1 h ...
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bioRxiv - Neuroscience 2022Quote: Total GLP1 protein in brain tissue or in plasma was determined using GLP-1 Total ELISA 96-Well Plate Assay (Sigma-Aldrich, EZGLP1T-36K). The assay was conducted according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... the workflow of this assay involves four main steps: (i) Tissue is harvested and lysed in lysis buffer containing S-Methyl Methanethiosulfonate (MMTS, Sigma, Cat no. 64306) to block free thiol groups (-SH) ...
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bioRxiv - Physiology 2022Quote: Tissue triglyceride levels were measured using an enzymatic triglyceride assay (Glycerol Reagent, Cat# F6428, Triglyceride Reagent, Cat#T2449, Sigma-Aldrich, Oakville, ON, Canada). In a flat bottom 96 well plate ...
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bioRxiv - Physiology 2024Quote: ... [NH3 + NH +]) was determined spectrophotometrically in 25 µL aliquots of flash frozen plasma by enzymatic ammonia assay (Sigma-Aldrich, USA, Catalog number: AA0100). [TAmm] was calculated from the delta absorbance at 340 nm wavelength before and after the addition of the enzyme L-glutamate dehydrogenase ...
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bioRxiv - Immunology 2024Quote: ... IL-31 and IL-33 cytokines with multiplexed-ELISA assay with Luminex 200 Multiplex Bio-Plex 200 System (EMD Millipore, Billerica, MA, USA) using a Milliplex Map kit (EMD Millipore).
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bioRxiv - Microbiology 2023Quote: Proteins were isolated from hPCLS with the radioimmunoprecipitation assay (RIPA) buffer (Thermo, Cat.# 89901) containing a cocktail of protease inhibitors (Sigma, Cat.# P8340-5ML) and phosphatase inhibitors (Fisher ...
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bioRxiv - Genetics 2023Quote: ... exact number recorded per vial for assay read-out) were then exposed to standard medium prepared with 200mM Mifepristone (RU486) from Sigma Aldrich (cat# 856177) and 2.5% w/v FD&C Blue Dye no 1 from Spectrum (cat# FD110 ...
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bioRxiv - Microbiology 2022Quote: ... The precipitates were extracted into 1x SDS sample buffer containing 2-mercaptethanol and subjected to a standard immunoblotting assay using HRP-conjugated anti-HA antibody (H6533; Sigma; 1:3000 dilution) and anti-FLAG antibody (A8592 ...
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bioRxiv - Microbiology 2022Quote: ... Iron concentrations were then normalized to the protein content in each sample using the bicinchoninic acid (BCA) assay (Sigma Aldrich, St. Louis, MO). Quantification was performed from 3 independent cultures for each strain.
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bioRxiv - Evolutionary Biology 2023Quote: ... three times with a sharp metal rod of the same diameter as the calibrated glass micropipettes used for the assays (5 μL, Sigma, product number P0549). Three glass micropipettes per vial were filled with liquid media ...
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bioRxiv - Plant Biology 2023Quote: ... Expression of the plasmid in floral tissue of T2 plants was confirmed by GUS staining assay following the manufacturer’s protocol (Millipore Sigma, Burlington, MA, USA) (Figure S6) ...
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bioRxiv - Cancer Biology 2023Quote: Proximity ligation assays (PLA) were performed according to manufacturer’s protocol for both flow cytometry and in situ confocal microscopy detection (Sigma, DUO94104 and DUO92013 respectively). For flow cytometry ...
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bioRxiv - Physiology 2024Quote: ... Concentrations of plasma TG in the arterial and venous samples were determined enzymatically using a commercially available assay (Sigma-Aldrich, St. Louis, MO) and by calculating an average concentration based on ten replicates.
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bioRxiv - Neuroscience 2024Quote: ... Cells were counted and seeded (400,000 cells/mL for survival assays) on poly-L-lysine treated glasses (MW 350 kDa, Sigma Chemical, St. Louis, MO). The feeding medium was replaced to growth media ...
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bioRxiv - Bioengineering 2024Quote: ... Metabolic and mitochondrial activity of host cells was assessed with a resazurin assay (7-Hydroxy-3H-phenoxazin-3-one-10-oxide sodium salt, Sigma-Aldrich, Overijse, Belgium). Medium was refreshed with 1 mL of 0.01 mg/mL resazurin in DMEM and incubated for 2 h at 37°C ...
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bioRxiv - Genetics 2024Quote: Snap frozen gastrocnemius or spinalis muscles were homogenised in 4% SDS lysis buffer and assessed for total protein concentration using Direct Detect Assay free cards (Millipore, Cat #DDAC00010-GR). Proteins were separated by SDS-PAGE using stain-free precast midi- criterion gels (Biorad) ...