Labshake search
Citations for Millipore Sigma :
8701 - 8750 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... The levels of secreted cytokines and chemokines in the collected effluents were measured using the MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel-Immunology Multiplex Assay from Millipore (Cat.# HCYTOMAG-60K), following the manufacturer’s directions ...
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bioRxiv - Immunology 2022Quote: ... the total DNA methylation signature was evaluated in 100 ng of DNA using the commercial kit (Imprint Methylated DNA Quantification kit, Sigma-Aldrich), according to the manufacturer’s recommendations.
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bioRxiv - Physiology 2020Quote: ... according to the manufacturer’s instructions (Mouse Leptin ELISA Kit, 90030; Crystal Chem, Zaandam, Netherlands; Rat/Mouse Insulin ELISA Kit, cat. EZRMI-13K; Merck Millipore). The intra- and interassay coefficients of variation (CVs ...
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bioRxiv - Bioengineering 2020Quote: ... Apoptosis was assessed by DNA fragmentation staining using a commercially available kit (In Situ Cell Death Detection Kit, TMR red, Sigma-Aldrich).
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bioRxiv - Neuroscience 2020Quote: ... revelation was performed using an avidin/biotin complex coupled with peroxidase (ABC kit, Universal Vectastain Kit PK6200) and diaminobenzidine (Sigma Aldrich).
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bioRxiv - Neuroscience 2020Quote: TUNEL was performed using a standard kit according to the manufacturer’s protocol (TMR In Situ Cell Death Detection Kit, Sigma-Aldrich, #12156792910) in combination with nuclear staining using DAPI (10 ug/mL ...
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bioRxiv - Cell Biology 2022Quote: RNA from different cancer cell lines was extracted using RNA isolation kit (Bio Rad) and treated with DNAse using DNAse 1 kit (AMPD1, Sigma-Aldrich). cDNA synthesis was done using a cDNA synthesis kit (Bio Rad) ...
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bioRxiv - Zoology 2020Quote: ... DNA was extracted from individual cysts or juveniles using the “Sigma Extract-N-Amp Kit (XNAT2)” kit (Sigma, St. Louis, MO) according to the protocol reported by Ma et al ...
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bioRxiv - Cell Biology 2023Quote: ... post-fixed in cooled ethanol:acetic acid (2:1) and stained using a TUNEL kit (ApopTag® Fluorescein In Situ Apoptosis Detection Kit, Merck Millipore, #S7110) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... All cells were regularly tested for the presence of mycoplasma using the PCR Mycoplasm Test Kit (PromoKine) or the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich).
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bioRxiv - Immunology 2024Quote: ... mRNA was isolated and converted into cDNA using a commercially available kit (GenElute Mammalian Total RNA Miniprep Kit; Sigma-Aldrich, USA). Transcript levels were determined by real-time quantitative polymerase chain reaction (QuantStudio 5 ...
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bioRxiv - Evolutionary Biology 2024Quote: Random-PCR amplification of the Viral DNA or RNA were respectively achieved with the GenomePlex Complete Whole Genome Amplification (WGA) Kit and the TransPlex Whole Transcriptome Amplification (WTA) Kit (Sigma-Aldrich) according the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 2 x 100% for 30 minutes each) and then embedded in epoxy embedding medium kit (Epon substitute embedding medium kit; 45359, Sigma Aldrich). For this ...
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bioRxiv - Microbiology 2022Quote: ... Cells were verified using the Promega GenePrint STR kit (DNA Analysis Facility, Duke University) and as mycoplasma free by the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich).
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bioRxiv - Developmental Biology 2023Quote: ... Cell proliferation was determined with the iClick EdU Imaging Kit (ABP Biosciences) and apoptosis was measured using the ApopTag Peroxidase in Situ Apoptosis Detection Kit (Millipore Sigma), following the manufacturers’ protocols ...
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bioRxiv - Bioengineering 2024Quote: ... device effluent collected from the maternal and fetal chambers was measured using the following ELISA kits: Human IL-1β ELISA Kit (RAB0273, Sigma Aldrich), Human IL-6 ELISA Kit (RAB0306 ...
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bioRxiv - Bioengineering 2024Quote: The measurements were conducted by following the manufacture of NADP/NADPH Quantification Kit (MAK038) and NAD/NADH Quantification Kit (MAK037) from Sigma Aldrich. 10 O.D ...
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bioRxiv - Neuroscience 2021Quote: ... at a density of 40,000 cells/well in 24-well plates for EdU incorporation assays or on 35-mm tissue culture dishes coated with poly-L-lysine (Sigma, P1524; 30 μg/mL) at a density 10×106 cells/dish for immunoblotting ...
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bioRxiv - Physiology 2020Quote: ... Fat tissues were homogenized in a modified radioimmunoprecipitation assay (RIPA) lysis buffer supplemented with 1% protease inhibitor mixture and 1% phosphatase inhibitor mixture (Sigma-Aldrich, St. Louis, MO). Tissue lysates were resolved by SDS-PAGE ...
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bioRxiv - Bioengineering 2021Quote: ... using a Milliplex Rat Gut Hormone Panel 96-well plate assay as described by the manufacturer (#RMHMAG-84K-06; EMD Millipore Corporation, Billerica, MA). Data was collected with MAGPIX with xPONENT software (Luminex ...
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bioRxiv - Biophysics 2021Quote: ... The enzyme/protectant mixture was added 1:10 to assay buffer (100 mM Sodium Phosphate, 2 mM Sodium Pyruvate [Sigma-Aldrich], 1 mM NADH [Sigma-Aldrich], pH 6). Enzyme kinetics were measured by NAD+ absorbance at 340 nm ...
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Long-Range Electrostatic Interactions Significantly Modulate the Affinity of Dynein for MicrotubulesbioRxiv - Biophysics 2021Quote: ... We 20-fold diluted the MTBD-coated beads in Tris-assay buffer with antifade reagents (125 nM glucose oxidase (G2133, Sigma-Aldrich, St. Louis, MO), 32 nM catalase (C9322 ...
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bioRxiv - Plant Biology 2021Quote: ... The H2O2 concentrations were determined by a standard curve developed using 0.25 – 2.5 μM and normalized by calculating the amount of protein in the extract using a standard Bradford Assay (Bradford, 1976) with Bradford Reagent (Sigma-Aldrich, cat no. B6916).
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bioRxiv - Microbiology 2020Quote: ... Duplicate aliquots (40 μL) of the resulting hemolysates were mixed with 80 μL assay buffer containing 80 μM porphobilinogen (Sigma Aldrich, Castle Hill, Australia) and incubated at 37 °C for 60 min to permit the PBGD-catalyzed generation of hydroxymethylbilane ...
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bioRxiv - Microbiology 2022Quote: ... assays22 and nanostructure-initiator mass spectrometry (NIMS)23 assays were performed on the supernatant fraction and the remaining material was filtered using Miracloth (Millipore Sigma, Burlington, MA, USA) and dried at 50 °C to determine the biomass dry weight.
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bioRxiv - Microbiology 2022Quote: ... hormones were maintained in the culture medium throughout the course of each assay. 17-ß-estradiol (i. e., E2) and progesterone (i. e., P4) (both from Sigma; St. Louis, MO) were selected for use in infection studies because these hormones represent the major forms of estrogens and of progestogens (respectively ...
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bioRxiv - Physiology 2020Quote: ... and 120 minutes thereafter and blood glucose concentrations were measured by colorimetric assay (PGO enzyme preparation and dianisidine dihydrochloride, Sigma-Aldrich, St. Louis, MO).
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Epigenetic Interaction between UTX and DNMT1 Regulates Diet-Induced Myogenic Remodeling in Brown FatbioRxiv - Physiology 2020Quote: ... Tissues were homogenized in a modified radioimmunoprecipitation assay (RIPA) lysis buffer supplemented with 1% protease inhibitor mixture and 1% phosphatase inhibitor mixture (Sigma-Aldrich, St. Louis, MO) and tissue lysates were resolved by SDS-PAGE ...
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bioRxiv - Cell Biology 2021Quote: Cells were lysed or tissue mechanically homogenised in lysis buffer (Extraction buffer, FNN0011) and protein content was quantified by a BCA assay (Sigma-Aldrich, St. Louis, MO). Twenty micrograms of protein were resolved on a 4-12% Bis-Tris gel (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... leaf discs were placed in a 96-well plate containing 50 μL of distilled water and 50 μL of assay solution containing 20 mM Luminol (Sigma-Aldrich, St. Louis, A8511), and 1 mg/mL peroxidase (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% [v/v] Triton X-100) and incubated at 37°C for 3 h either with α-mouse plus and α-rabbit minus for PLA assay (Sigma-Aldrich, Duolink). PLA samples were then washed with MTSB and incubated for 3 h at 37°C with ligase solution ...
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bioRxiv - Cancer Biology 2024Quote: ... supernatants were analyzed using MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel - Premixed 25 Plex - Immunology Multiplex Assay (EMD Millipore Corporation, Billerica, MA, USA). and custom FAS panel on the Luminex MAGPIX ...
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bioRxiv - Neuroscience 2022Quote: Brain punches were homogenized in radioimmunoprecipitation assay (RIPA) buffer supplemented with phosphatase inhibitor cocktail 1 and 2 and protease inhibitor cocktail (Sigma Aldrich, Saint Louis, MO) followed by centrifugation at 16,000 g for 20 min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: Inflammatory proteins were measured using the MILLIPLEX Mouse Cytokine/Chemokine Magnetic Bead Panel – Premixed 32 Plex – Immunology Multiplex Assay (EMD Millipore. Corporation, Billerica, MA). Calibration curves from recombinant cytokine standards were prepared with threefold dilution steps in the same matrix as the samples ...
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bioRxiv - Biochemistry 2024Quote: ... Extracellular acidification rate (ECAR) measurements were collected and normalized to protein content that was determined by Pierce™ BCA Protein Assay (Sigma, Catalog number 23228). Glycolysis ...
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bioRxiv - Cancer Biology 2024Quote: ... Panel balance and individual [Nuc:bead] identity were confirmed in Luminex assays with anti-dsDNA (EMD Millipore #MAB030; 1/5, 1/50 and 1/500), anti-H3.1/2 (Active Motif #61629 ...
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bioRxiv - Immunology 2023Quote: ... Thirty-two markers were simultaneously measured in the samples using Eve Technologies’ Mouse Cytokine 32-Plex Discovery Assay® (Millipore Sigma, Burlington, Massachusetts, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... cell death detection enzyme-linked immunosorbent assay PLUS (ELISA) and human anti-β-actin antibody were obtained from Sigma-Aldrich (Saint-Quentin-Fallavier, France). Poly-ADP-ribose polymerase (PARP ...
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bioRxiv - Bioengineering 2022Quote: ... total mRNA was measured using the RiboGreen assay with an upfront lysis step in 2% Triton X-100 (SX100-500ML; Sigma Aldrich, St. Louis, MO) to lyse the LNPs ...
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bioRxiv - Physiology 2022Quote: The subcellular distribution and possible spatial association between S-NO and Cx43 were evaluated by Proximity Ligation Assay(Soderberg et al., 2006) (Sigma, St. Louis, MO, USA). Tissue sections (6 μm ...
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bioRxiv - Neuroscience 2023Quote: ... cells were washed twice with PBS at room temperature (RT) and lysed in 1x (radio immunoprecipitation assay buffer (RIPA buffer, Merck Millipore, cat. no. 20-188) containing 0.1% SDS ...
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bioRxiv - Genomics 2024Quote: DNA indexes with different overhang sequences were validated by performing in-situ ligation assays on 4T1 mouse cells grown on 25mm round coverslips coated in poly-L-lysine overnight (Sigma P4707, 0.01% w/v). Cells were grown for 24-48hrs to ∼90% confluence.
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bioRxiv - Biochemistry 2024Quote: ... In our studies the experimental rapid Mg2+ influx was normalized to the control (Eq 1): In some assays a final concentration of 1 mM Co(III)Hex (Sigma Aldrich, # 50-180-0331) was added to inhibit Mg2+ import but not export ...
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bioRxiv - Immunology 2024Quote: ... Forty-eight markers were simultaneously measured in the samples using Eve Technologies’ Human Cytokine Panel A 48-Plex Discovery Assay (Millipore Sigma, Burlington, Massachusetts, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies for DNA damage assays were diluted in PBS with 10% SuperBlock at the following dilutions: anti-human γH2AX Ser139 (EMD Millipore; JBW301; 1:200), goat anti-mouse IgG AF546 (Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: ... Tissue triacylglycerol abundance was measured using a colorimetric assay (Glycerol Reagent, Cat. No. F6428, Triglyceride Reagent, Cat. No. T2449, Sigma-Aldrich, Oakville, ON, Canada) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: A 1 ml syringe with a 34-gauge needle was used to penetrate the tympanic membrane to inject 100 mg ml-1 sodium arsanilate (A9528 Sigma-Aldrich, 40 μl, prepared in 0.3 M NaHCO3) into the middle ear ...
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bioRxiv - Genetics 2021Quote: ... Mix-n-Stain CF 555 Antibody Labeling Kit and Mix-n-Stain CF 647 Antibody Labeling Kit (Sigma-Aldrich, MX555S100 and MX647S100) were used according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2020Quote: Primary cultures fixed and permeabilised as described for IF were used to perform PLA using a commercial kit (Duolink® PLA starter kit, Sigma-Aldrich) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA of control and insertion lines was extracted using a commercial kit (Spectrum™ Plant Total RNA Kit, Sigma life science) following the supplier’s instructions ...