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Citations for Millipore Sigma :
8551 - 8600 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Simultaneous determination of nitrate and nitrite concentrations induced by the bacteria was done by Vanadium assay with the reduction of nitrate to nitrites by Vanadium (III) combined with detection by the acidic Griess reaction (Sigma-Aldrich, USA), as reported in Miranda et al. ...
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bioRxiv - Cell Biology 2021Quote: ... and mouse anti-Flag (1:1,000 for western blotting and 2 μg for co-IP assays; F1804; Sigma, St. Louis, MO, USA). Primers and sequence information are provided in Supplementary Table S1.
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bioRxiv - Microbiology 2020Quote: The fluorescent substrate MUC was applied for the Cts1 liquid assay (4-methylumbelliferyl β-D-N,N′,N′′-triacetylchitotrioside hydrate; M5639 Sigma-Aldrich) (Koepke et al. ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Serum was collected from groups of mice and an in–house assay was used in which anti–human IgG Fc (Sigma, Catalog # FAB3700259) was used to capture human IgG (MS-Hu6) ...
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bioRxiv - Biochemistry 2020Quote: ... cell lysates were diluted 10-fold and incubated with substrate (5 mM α-naphthyl phosphate, Sigma-Aldrich N7255, dissolved in assay buffer) at 30°C for 20 min ...
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bioRxiv - Evolutionary Biology 2022Quote: The enzyme assay for the zebrafish Irg1-like was performed by incubating 7 µg recombinant protein with 1 mM cis-aconitate (Sigma A3412-1G) in 300 µl reaction buffer (10 mM HEPES ...
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bioRxiv - Cancer Biology 2022Quote: Cell proliferation was assessed by the colorimetric assay of sulforhodamine B 2-(3-diethylamino-6-diethylazaniumylidene-xanthen-9-yl)-5-sulfo-benzenesulfonate (SRB) (Sigma Aldrich, USA)[25] ...
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bioRxiv - Cell Biology 2022Quote: To evaluate the angiogenic potential of BEC CM a tube formation assay was performed using the HCMEC/D3 line (SCC066 Merck Millipore, Darmstadt, Germany), as described above ...
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bioRxiv - Physiology 2020Quote: ... and then levels of glucose in both duplicates measured by colorimetric assay following the addition of a glucose oxidase reagent (Sigma; GAGO-20). Levels of glycogen in each experimental sample were then calculated by subtracting the glucose measurements of the untreated duplicate from the amyloglucosidase-treated sample ...
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bioRxiv - Physiology 2021Quote: Cortisol concentrations were measured in mouse sera by a solid phase competitive enzyme linked immunosorbent assay (ELISA) (Sigma-Aldrich Co Ltd, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: A MilliPlex MILLIPLEX® MAP Human Cytokine/Chemokine/Growth Factor Panel A-Immunology Multiplex Assay (HCYTA-60K-13, Millipore Sigma, Burlington, Massachusetts) was run on a Luminex 200 Instrument (LX200-XPON-RUO ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Coating solution was then removed by flicking and plates were washed with OptiMEM and blocked with 25 ul/well of assay buffer (1% Ovalbumin (Sigma #A5503-10G) in OptiMEM containing 1X Pen-Strep (Gibco #15140-148)) ...
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bioRxiv - Physiology 2021Quote: ... and then levels of glucose in both duplicates measured by colorimetric assay following the addition of a glucose oxidase reagent (Sigma; GAGO-20). Levels of glycogen in each experimental sample were then calculated by subtracting the glucose measurements of the untreated duplicate from the amyloglucosidase-treated sample ...
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bioRxiv - Immunology 2020Quote: IgG mediated antibody titer of serum samples collected from immunized animals and convalescent patients’ sera were determined by enzyme-linked immunosorbent assay (ELISA)35 using 1:5000 dilution of HRP conjugated anti mouse (A4416, Sigma-Aldrich, USA) and 1:10000 dilution of HRP conjugated anti human secondary antibody (A0170 ...
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bioRxiv - Cancer Biology 2021Quote: ... and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and urethane (ethyl carbamate, EC; CAS# 51-79-6) from Sigma (St. Louis, MO). Primer sets were Kras ...
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bioRxiv - Microbiology 2020Quote: ... aureus binding to fibronectin and fibrinogen was determined using a semi-quantitative adherence assay on 96-well tissue culture plates (Sigma-Aldrich, USA). Plates were coated with 100 μl of 0.02% sodium carbonate (pH 9.6 ...
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bioRxiv - Cancer Biology 2022Quote: The spheroid formation assays were carried out on a low attachment U-bottom 96 well plate (BRAND® 96-well microplate, Sigma Aldrich). Single-cell suspension of 500 parental and topotecan resistant Y79 cells in 10% RPMI medium was loaded in each well of a 96 well plate followed by centrifugation for 1000rpm for 1 min to facilitate cell aggregation ...
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bioRxiv - Cell Biology 2022Quote: ... The protein concentration was determined using BCA™ protein assay (Pierce, Rockford, IL, USA) using BSA (Sigma- Aldrich, St. Louis, MO, USA) as a standard ...
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bioRxiv - Biophysics 2022Quote: The activity of β-galactosidase was measured using a spectrophotometric assay in which o-Nitrophenyl-β-D-Galactopyranoside (ONPG, CAS Nr: 369-07-3 Calbiochem Sigma-Aldrich) was used as a substrate ...
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bioRxiv - Cancer Biology 2022Quote: Blast colony forming cell (Bl-CFC) assays were performed using iPSC cell pellets resuspended in Stemline II Hematopoietic Stem Cell Expansion medium (Sigma-Aldrich S0192) supplemented with 1% penistreptomycin and L-glutamine ...
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bioRxiv - Immunology 2022Quote: ... IgG3 and IgM) in mice were determined after infection with MORV and VSV using mouse Isotyping multiplex assay (MGAMMAG-300K, Millipore Sigma, USA) by Luminex xMAP technology as recommended by the manufacture.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... magnetic force was applied by placing the assay plates on a handheld magnetic separation block (V&P Scientific 771HH-H/Millipore 40-285). In all subsequent experiments ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration in samples was determined using Bradford assay (Bradford, 1976) against standards of known protein concentration prepared using bovine serum albumin (BSA; Sigma-Aldrich, UK).
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bioRxiv - Molecular Biology 2024Quote: The standard assay for ALP activity was carried out in 500 μL of the reaction mixture containing 2 mM pNPP (Sigma-Aldrich, USA) in 0.1 M Tris–HCl buffer ...
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bioRxiv - Immunology 2024Quote: ... MASP-3 and MAP-1 was measured in an ELISA assay where 10 µg/ml recombinant human albumin (rHA) (Sigma-Aldrich, A9731) was coated on Nunc MaxiSorp flat-bottom 96-well plates (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Then 20 µl of the mix was transferred to a tube containing 10 µl 3x Stop buffer (assay buffer + 100 mM EDTA, 500 µM proteinase K (Sigma-Aldrich, 107393), 1% SDS ...
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bioRxiv - Microbiology 2023Quote: ... Lung homogenates were clarified of debris by centrifugation at 800 xg for 5 minutes then aliquots were frozen at −80°C neat for plaque assay or diluted at a 1 to 4 ratio in TRIzol (Sigma-Aldrich, MA) for RNA isolation and quantitative PCR ...
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Doxorubicin inhibits phosphatidylserine decarboxylase and confers broad-spectrum antifungal activitybioRxiv - Pathology 2023Quote: The Psd enzyme activity assay based on fluorescence was performed with 1,2-diacetyl benzene/β-mercaptoethanol (1,2-DAB/β-ME) (Sigma-Aldrich, Germany), which forms a fluorescent iso-indole-mercaptide conjugate with PE ...
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bioRxiv - Molecular Biology 2022Quote: HEK293T cells (used for drug susceptibility assays) were cultured in plating medium (FreeStyle F17 supplemented with 10% FBS, 4 mM L-glutamine, 1x PenStrep (Sigma, Cat#P4458)) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cytokine levels in PBMC culture supernatants were measured using the MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel-Custom 6 Plex-Immunology Multiplex Assay (Millipore; #HCYTOMAG-60K). Analytes included were the following ...
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bioRxiv - Microbiology 2023Quote: ... The proximity Ligation Assay (PLA) was then performed using the Duolink® In Situ Detection Red as recommended by the manufacturer (Sigma Aldrich). After the PLA reaction was performed ...
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bioRxiv - Genetics 2023Quote: ... The IC90 at day 6 of treatment for each drug was determined by treating the inducible SpCas9 single clone (before gRNA library infection) with a dose curve of each drug in triplicate and taking an MTT assay measurement (Sigma Aldrich 11465007001) on day 6 following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... the entire frozen neocortex of the left hemisphere was grinded by Dounce homogenizer in RIPA buffer (radioimmunoprecipitation assay buffer, 20-188, Millipore, CA, USA) in the presence of protease inhibitors and phosphatase (Roche ...
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bioRxiv - Bioengineering 2023Quote: ... 90 μL of the remaining solution after ALP assay was mixed with 90 μL of digestion buffer (500 mM phosphate buffer, 5 mM L-cystein (Sigma-Aldrich, C1276), 5 mM EDTA (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... 250 ng of pTOPflash (wt-TCF) or pFOPflash (mutated-TCF binding site, negative control) reporter plasmids (TOPflash luciferase reporter assay, Merck Millipore, Darmstadt, Germany) were transfected with Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... Supernatants containing soluble proteins fraction were transferred in a new Eppendorf tube of 1.5mL and the total protein concentration was determined by BRADFORD assay (Sigma-Aldrich, ref 23238).
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bioRxiv - Physiology 2023Quote: ... containing 12.5µL of assay buffer (125mM KCl, 20mM HEPES, 2mM MgCl2, 2mM KH2PO4, pH =7.4) supplemented with 40mM succinate (Sigma-Aldrich # S3674-100G), 40mM malate (Sigma-Aldrich #240176-50G) ...
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bioRxiv - Immunology 2023Quote: ... Cells were then treated in triplicates for 24hrs with varying concentrations of rhIL-23 for analysis via a WST-1 proliferation assay using WST-1 reagent (0501594400, Sigma-Aldrich, Canada) and an apoptosis assay using Caspase-Glo® 3/7 reagent (G8091 ...
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bioRxiv - Molecular Biology 2024Quote: ... were transferred to a 96-well plate and overlayed with 120 µL of assay buffer containing DTNB (D8130, Sigma; 2 mg/ 3mL) and 10 U glutathione reductase (G3664 ...
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bioRxiv - Physiology 2024Quote: ... Assay-specific metabolic compounds were diluted day-of in XF DMEM media immediately prior to experiments: Oligomycin 5μM (Sigma Aldrich cat. 495455), Rotenone 2.5μM (Sigma Aldrich cat ...
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bioRxiv - Systems Biology 2024Quote: ... or thawed on ice from frozen preparations and 25 μg from each sample was prepared for glutamate dehydrogenase assay according to manufacturer instructions (Millipore Sigma MAK099). The absorbance at 450 nm was then measured every two minutes for 4 hours on Cytation 5 (Biotek ...
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bioRxiv - Cell Biology 2024Quote: Interactions were then quantified by β-D-galactosidase activity using the o-nitrophenyl– β-D-galactopyranoside (ONPG) assay (48712-M; Sigma Aldrich) according to the published methods (Amberg et al. ...
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bioRxiv - Microbiology 2024Quote: Cation-Adjusted Muller Hinton Broth (Muller Hinton 2, MH2) was used as the culture medium for all assays (22 g/l; Sigma-Aldrich, UK). For assays requiring solid medium ...
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bioRxiv - Cancer Biology 2024Quote: ... the pellets were stained for 20 minutes in 100 µL of assay buffer containing 5 µg/mL propidium iodide (Sigma-Aldrich P4170) and 3 µL of Annexin V-FITC (Biolegend 0640906) ...
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bioRxiv - Molecular Biology 2020Quote: Proteins were separated by size through a sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto a polyvinylidene difluoride (PVDF) membrane (Immobilon-P, Millipore, 0.45 µm pore size; CAT. #IPVH00010) previously methanol-equilibrated ...
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bioRxiv - Physiology 2020Quote: ... and each circulating hormones were determined using a commercially available ELISA kit for total ghrelin (Rat/Mouse Total Ghrelin ELISA kit, EZRGRT-91K, Millipore), and active ghrelin (Rat/Mouse Total Ghrelin ELISA kit ...
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bioRxiv - Cancer Biology 2022Quote: ... EV were labeled with a PKH67 dye labeling kit-green (or PKH26 dye labeling kit-red) following the manufacturer’s instructions (Sigma-Aldrich). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... Bacteriophage DNA was isolated using the Phage DNA Isolation Kit (Norgen) and plasmids were isolated with the GenEluteTM Plasmid Miniprep Kit (Sigma). DNA purification from agarose gel or enzymatic reactions was performed with the NucleoSpin Gel and PCR clean up kit (Macherey-Nagel) ...
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bioRxiv - Plant Biology 2024Quote: ... Each construct was transformed into a yeast sucrose invertase (suc2) mutant using a yeast transformation kit following the manufacturer’s instructions (Yeast Transformation Kit, Sigma-Aldrich). The suc2 mutant is unable to secrete the SUC2 protein and thus cannot grow on media containing sucrose (Suc ...
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bioRxiv - Plant Biology 2020Quote: ... using the GenElute Plasmid Miniprep Kit (Sigma-Aldrich). Inserts were sequenced (GATC Services ...