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Citations for Millipore Sigma :
651 - 700 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... All cell lines were routinely tested for mycoplasma contamination using LookOut Mycoplasma qPCR Detection Kit (Sigma, MP0035-1KT).
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bioRxiv - Developmental Biology 2019Quote: ... Cell death was measured by TUNEL assay using the ApopTag® Plus Fluorescein In Situ Detection kit (Millipore). Fluorescently stained sections were imaged on a Zeiss LSM710 confocal microscope.
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bioRxiv - Cell Biology 2020Quote: TUNEL staining was carried out by using an ApopTag® Fluorescein in Situ Apoptosis Detection Kit (S7110, Sigma) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: Oxidized proteins containing carbonyl groups were detected using the OxyBlot Protein Oxidation Detection Kit (Sigma-Aldrich: cat#S7150). Briefly ...
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bioRxiv - Bioengineering 2022Quote: ... we cultured T-SLICE in the presence of 100 μM of the EF5 Hypoxia Detection Kit (Sigma-Aldrich). Cell culture media was supplemented with 100 μM EF5 incorporated into the media for 6 ...
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bioRxiv - Cell Biology 2023Quote: Protein carbonyl formation by oxidative reactions was assessed with the OxyBlot Protein Oxidation Detection Kit (S7150, Merck Millipore), according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... The HEK cells used in this study were found free of mycoplasma infection (Sigma-Aldrich mycoplasma detection kit). These cells were cotransfected with 0.1 - 1 μg of mutant hHV1-TAG plasmid and 0.7 μg of pAnap plasmid (a gift from Dr ...
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bioRxiv - Cell Biology 2023Quote: ... iNs were subjected to in situ PLA using Duolink In Situ Detection Reagents Red kit (Sigma, catalog #: DUO92008) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... allowing for detection of the secondary HRP antibody detected using a chemiluminescence peroxidase substrate kit (Sigma, #CPS-1) and subsequent detection of the GAPDH loading control detected using its laser based infrared detection system ...
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bioRxiv - Neuroscience 2023Quote: ... TUNEL-positive cells were stained using the in-situ cell death detection kit TMR red (11684795910; Sigma-Aldrich) following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... Cells were fixed in 4% PFA for 2 min and stained with Alkaline Phosphatase Detection Kit (Sigma-Aldrich). Colonies were manually counted distinguishing between differentiated ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 50 µL of eluate was measured with an colorimetric iron detection kit (Sigma-Aldrich, Cat. No. MAK025-1KT) following the provided protocol ...
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bioRxiv - Physiology 2024Quote: ... Cell lines were tested at least twice a year for Mycoplasma contamination using the LookOut Detection Kit (Sigma), and all tests were negative.
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bioRxiv - Microbiology 2019Quote: ... contained in the Kap2G Robust PCR kit (Sigma Catalogue No. KK5005) per sample and 11 µL added to each well need to be used in a 96-well plate ...
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bioRxiv - Genomics 2022Quote: ... contained in the Kap2G Robust PCR kit (Sigma Catalogue No. KK5005) per sample and 11 µL added to each well need to be used in a 96-well plate ...
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bioRxiv - Microbiology 2023Quote: ... then purified using GenElute PCR Clean-Up Kit (Sigma, SKU NA1020), and KAPA Pure Beads (Roche-07983298001) ...
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bioRxiv - Microbiology 2023Quote: ... PCR amplification was performed using KOD Hotstart polymerase kit (EMD Millipore) and primers flanking the gRNA target sites (Table 2) ...
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bioRxiv - Cell Biology 2020Quote: ... are stained for 5 minutes at room temperature RT with 0.5μl of PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling (#P7333, Sigma) and then polished with the exosome spin columns (#4484449 ...
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bioRxiv - Neuroscience 2021Quote: ... 350μl of RNAzol RT (Sigma-Aldrich) were added to the samples and lysis was performed with TissueLyser II (Qiagen ...
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bioRxiv - Genetics 2019Quote: ... 50 uL of PCR product of each sample was purified with SpinPrep PCR Clean-Up Kit (EMD Millipore Corp) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... the tagmented DNA was amplified by PCR using the KAPA2G Robust PCR kit (Sigma-Aldrich, St. Louis, MO, USA) using the GC buffer along with 0.2 μM of each custom P5 and P7 indexing primers (File S1 ...
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bioRxiv - Synthetic Biology 2020Quote: ... Template plasmid was digested using DpnI and the PCR products were purified using a PCR purification kit (Sigma-Aldrich). Subsequently ...
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bioRxiv - Developmental Biology 2021Quote: ... embryos are rinsed in HB-buffer (HB+ without torula RNA and heparin) supplemented with saline-sodium citrate buffer (SSC, Sigma, S6639) and then transferred gradually to PBT ...
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bioRxiv - Genomics 2020Quote: ... The complete reaction mix was again removed and a slide wash with one 10 minute wash of the wells with 2X SSC/0.1% SDS (Sigma-Aldrich, USA), followed by 1 minute wash with 0.2X SSC and finally 0.1X SSC was performed ...
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bioRxiv - Bioengineering 2021Quote: ... in PBS was incubated in each well for 5 minutes and cells were washed with 2x SSCT (2x SSC with 0.1% TWEEN® 20 (P9416-100ML; Sigma-Aldrich). For storage ...
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bioRxiv - Cell Biology 2021Quote: ... The blot was then neutralized with SSC and hybridized with a DIG-labeled telomere probe prepared as described 48 in DIG Easy Hyb (Sigma-Aldrich) solution overnight at 42°C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the preparations were thermally aged overnight at 37 °C and then 60 min at 60 °C, followed by treatments with RNase A (200 µg/mL in 2× SSC, 60–90 min, 37 °C) (Sigma-Aldrich) and then pepsin (50 µg/mL in 10 mM HCl ...
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bioRxiv - Developmental Biology 2019Quote: ... samples were washed with wash buffer (10% formamide in 1X SSC and 0.1% Tween-20) for 5 min, then incubated with the hybridization mix (10% formamide, 1X SSC, 10% Dextran sulfate (w/v) (Sigma, D8906), 100nM each in situ probe ...
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bioRxiv - Genomics 2022Quote: ... before slides were detached from their hybridization chambers and washed for 10min at 50°C in 2X SSC/0.1% SDS (Sigma-Aldrich, USA), followed by 1 minute wash with 0.2X SSC and finally 0.1X SSC ...
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bioRxiv - Microbiology 2022Quote: ... Cells were subsequently washed twice with PBS and once with prehybridization buffer (10% deionized formamide [Millipore] in 2x concentrated SSC [Gibco]) for 5 min ...
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bioRxiv - Synthetic Biology 2024Quote: ... 50 nM readout probes in 10% EC Buffer (1 mL of ethylene carbonate, 7 mL Ultrapure RNase/DNase free water, 2 mL 20x SSC, and 1g Low MW dextran sulfate Sigma #D4911) was added to the cells and incubated for 20 minutes at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were incubated in 20x SSC at 65 °C and stained for 20 min with 5% Giemsa solution (Sigma-Aldrich, GS500).
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bioRxiv - Cell Biology 2023Quote: ... Samples were then pre-hybridized for 20 minutes at 37°C in wash buffer (2x SSC, 10% formamide (Sigma-Aldrich, F9037)) ...
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bioRxiv - Immunology 2023Quote: ... the gel was rinsed again with water and soaked in 10X SSC for 30-60 min before capillary transfer to an ImmobilonP membrane (Millipore Sigma), as described previously120 ...
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bioRxiv - Systems Biology 2023Quote: The coverslip was equilibrated in 1 ml of 30% formamide buffer composed of 2×SSC and 30% deionised formamide (Sigma, F9037) in a 12-well plate for >5 min prior to hybridisation ...
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bioRxiv - Systems Biology 2023Quote: ... the disulfide bond linking the probe and the fluorophore was cleaved with Cleavage buffer comprising 2×SSC and 50 mM TCEP (Sigma, 646547) for 15 min ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% TritonX100 in 2 × SSC), anti-bleaching buffer (50 mM Tris-HCl pH 8, 300 mM NaCl, 3 mM Trolox (Sigma 238813), 0.8% D-glucose (Nacalai Tesque 168060-25) ...
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bioRxiv - Molecular Biology 2023Quote: ... An anti-bleaching buffer (50 mM Tris-HCl pH 8.0, 300 mM NaCl, 2×SSC, 3 mM trolox (Sigma-Aldrich, 238813), 0.8% D-(+)-Glucose (Nacalai Tesque ...
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bioRxiv - Cell Biology 2024Quote: ... Coverslips were rehydrated in 2xSSC and then washed twice in pre-hybridization buffer (2x SSC + 5% formamide (Sigma #344206-100ML-M)) for 5 minutes each ...
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bioRxiv - Systems Biology 2021Quote: ... and miR-290-295_KO cluster mESC lines (129/Ola background) were cultured in Dulbecco’s Modified Eagle Media (DMEM) (Sigma-Aldrich), containing 15% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2019Quote: ... Eighteen microliters of high-titer lentivirus expressing miR-382 mixed with 2 μL 0.2% Evans blue dye (Sigma-Aldrich) in PBS was injected in the third mammary gland using a 34-gauge needle (Hamilton) ...
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bioRxiv - Neuroscience 2024Quote: ... Maternal Inflammatory Response (MIR) was induced with low-dose lipopolysaccharide (LPS, E. coli serotype 0111:B4; 0.008 mg/kg, Sigma) administered in a single I.P ...
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bioRxiv - Microbiology 2022Quote: ... The PCR products were purified using the kit Gene eluteTM Gel extraction kit (Sigma Aldrich, MA, USA), re-surveyed on Nanodrop® and delivered for Sanger DNA sequenced at Eurofins Genomics (Ebersberg ...
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bioRxiv - Developmental Biology 2021Quote: ... Rats were screened for the presence of mutations by PCR using tail-tip DNA samples (RED extract-N-Amp Tissue PCR Kit, Millipore-Sigma) as described previously (1 ...
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bioRxiv - Neuroscience 2022Quote: Genotyping for the R1.40 transgene locus was done with the recommended PCR primers using the PCR Ready Mix kit (E3004; Sigma-Aldrich). The sequences of the genotyping primers were as follows ...
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bioRxiv - Genetics 2021Quote: ... Purification of genomic Polymerase Chain Reaction (PCR) amplified DNA was performed with the GenElute PCR Clean-Up kit (Sigma-Aldrich) or the GeneJET PCR Purification Kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: Apoptotic cells were visualized with the terminal deoxynucleotidyl transferase (TdT)-FragEL DNA Fragmentation Detection Kit (Millipore, Etobicoke, ON, Canada) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... Ligation and amplification steps of the PLA were performed using the Duolink in situ Detection Reagents Orange kit (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... using ortho-nitrophényl-β-galactoside as substrate (β-Galactosidase Reporter Gene Activity Detection Kit, Sigma St Louis MI USA).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was performed according to manufacturer’s instructions using ApopTag Fluorescein In Situ Apoptosis Detection Kit (Millipore).