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Citations for Millipore Sigma :
7801 - 7850 of 8516 citations for V Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... neurons were cultured in 24-well plates on 13 mm coverslips coated with 1 mg/ml poly-D-lysine (Sigma) and 1 μg/ml laminin (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The plate was washed three times and 100 μl/well of SigmaFast o-Phenylenediamine dihydrochloride (OPD) developing solution (Sigma Aldrich) were added ...
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bioRxiv - Molecular Biology 2021Quote: ... These wells were washed five times using 1× PBST and followed by addition 50 μl/well of anti-human IgG horseradish peroxidase (HRP) (Sigma) diluted in Stabilzyme Noble (Surmodics) ...
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bioRxiv - Developmental Biology 2020Quote: ... 100 μL of luciferase assay cocktail was added to each well of lysate (25 mM Tris-Cl pH 7.8, 15 mM MgSO4, 10 mM ATP (Sigma-Aldrich), 65 μM BD D-luciferin-Potassium Salt (Fisher Scientific) ...
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bioRxiv - Developmental Biology 2020Quote: ... Cas9 mRNAs and sgRNA (50 ng/μl) was injected into the cytoplasm of fertilized eggs with well recognized pronuclei in M2 medium (Sigma). The injected zygotes were cultured in KSOM with amino acids (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.5×106 cells were mixed in a well of a 12-well plate with varying concentrations of supernatant containing viral particles and 8 μg/ml of polybrene (Sigma) which was then centrifuged at 2,000 rpm for 30 minutes at 30°C.
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bioRxiv - Cancer Biology 2022Quote: Lentivirus was titrated into 2 × 105 PC-3M cells on 6-well plates with 2 mL complete RPMI-1640 supplemented with 8 μg/mL polybrene (Sigma). After overnight infection ...
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bioRxiv - Neuroscience 2021Quote: EdU staining was performed on in vitro cell culture slides or on glass coverslips in 24-well plates which were precoated with poly-l-lysine (Sigma) and laminin (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2021Quote: ... cut into three pieces per mouse and incubated in 24-well plates in DMEM with high glucose supplemented with 2% wt/vol FA-free BSA (FA-free BSA DMEM, Sigma). BAT was then incubated for 1 hour at 37 °C before stimulation with 10 μM isoproterenol (ISO ...
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bioRxiv - Cell Biology 2022Quote: ... neurons were cultured in 24-well plates on 13 mm coverslips coated with 1 mg/mL poly-D-lysine (Sigma) and 1 µg/mL laminin (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... gastrocnemius muscles were enzymatically digested in a well containing 0.18% collagenase type I in Dulbecco modified eagles medium (DMEM) (Sigma-Aldrich) for ~2.5 hours ...
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bioRxiv - Cell Biology 2022Quote: ... Seahorse XFp cell culture miniplate was coated with 50 μg/ml poly-D-lysine (50 ul each well) (Sigma-Aldrich) for 30 min at room temperature and then aspirated followed by air drying at room temperature for 2 h ...
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bioRxiv - Immunology 2022Quote: Monocytic THP-1 cells were infected at a density of 2×105 cells/ml in 48 well plates in the presence of polybrene (8 μg/ml, Sigma). Infection levels were assessed at 48 h post-infection through enumeration of GFP positive cells by flow cytometry ...
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bioRxiv - Genomics 2020Quote: ... and the remaining adherent bacteria were stained with 2□ml/well of crystal violet (0.7% [wt/vol] solution; Sigma-Aldrich) for 12□min ...
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bioRxiv - Cell Biology 2020Quote: Wildtype and emerin-deficient H2K myogenic progenitors were seeded onto 12 well tissue culture plates coated with 0.01% gelatin (Sigma-Aldrich) for differentiation induction ...
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bioRxiv - Immunology 2021Quote: ... the following chemicals were added into the medium for each well depending on the dose used for that well for a total of 600μL cell medium/chemical mix around each dome: Rotenone (Sigma R8875), 2-Deoxy-D-Glucose (Sigma D8375) ...
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bioRxiv - Cell Biology 2019Quote: Cells were seeded onto 24-well insert plates with PET) permeable membranes with 1.0 uM pore size (Millipore product # PSRP010). Seeding volume was 300uL cells at density of .4×106 cells/mL (or 120,000 cells/well ...
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bioRxiv - Microbiology 2019Quote: ... Each of the five strains were added to a separate well containing 90 μL of 1/10th ISP2 with 1.25% purified agar (Sigma-Aldrich). The surface of each well was imaged for 48 hours using a Nikon Eclipse Ti microscope with a 20x phase contrast lens ...
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bioRxiv - Neuroscience 2019Quote: Prior to use 48-well MEA plates (Axion BioSystems) were coated overnight with 50 μg/mL poly-D-lysine (Sigma) in 0.1M borate buffer (pH 8.5) ...
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bioRxiv - Neuroscience 2019Quote: Cortices of P1 pups were dissociated by trypsin digestion and seeded onto 24-well plates coated with poly-D-Lysine (Sigma). Cultures were maintained for 4 days in Neurobasal (Invitrogen ...
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bioRxiv - Neuroscience 2019Quote: ... 300 μl per well and were lysed with 300 μl per well of 1% Nonidet P-40 lysis buffer containing proteinase inhibitor (1:10,000; Sigma-Aldrich). Samples were further homogenized using a Q125 sonicator (Qsonica ...
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bioRxiv - Developmental Biology 2019Quote: ATDC5 were plated in 6-well plates to 80% confluency and lentiviral transduction was using diluted viral media with 0.1% polybrene (EMD Millipore) for 24 hours followed by selection with 4μg/ml Blastocidin and Puromycin for 5 days post transfection ...
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bioRxiv - Cancer Biology 2019Quote: ... For cytokine analysis replicate wells were harvested on day +3 of co-culture and cytokine secretion determined via multiplex Luminex LiquiChip (Millipore).
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bioRxiv - Molecular Biology 2019Quote: ... Each plate also contained three wells of negative control cells treated with DMSO and a well of positive control cells treated with 20µM I-BET151 (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2019Quote: ... the viral supernatant was collected and added to cells in six-well plates with medium containing 8 μg/ml polybrene (Sigma). After 2 days’ infection ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos at the 60 hpf stage were aliquoted at three embryos per well into MultiScreen-Mesh mesh-bottomed plates (Millipore) and transferred to the drug plate (receiver tray ...
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bioRxiv - Genomics 2021Quote: ... as well as two monoclonal anitbodies targeting the KMT2A C-terminus: Mouse anti-KMT2A (1:100, Millipore Cat #05-765) refered to as KMT2A-C1 ...
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bioRxiv - Immunology 2021Quote: ... IgG was detected using goat-anti-human-Fc-AP (1:1000) (Jackson, catalogue no. 109-055-098) and wells were washed with PBS-T and AP substrate (Sigma) was added and plates read at 405 nm ...
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bioRxiv - Microbiology 2021Quote: Plaque size analysis were performed in HEK293T Spir-1 WT and KO cells seeded in 6-well plates coated with poly-D-lysine (Sigma). Once confluent ...
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bioRxiv - Microbiology 2021Quote: ... The withdrawn volume was recorded in each operation and approximately 10 ml of culture medium from positive wells were filtered through disposable 0.22 μm polysulfone filters (Millipore Sigma). DNA was extracted using a modified phenol-chloroform method (Urakawa et al. ...
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bioRxiv - Cell Biology 2020Quote: HeLa or HEK293T cells were grown on coverslips in the 24-well plate and HEK293T cells were pre-coated with PLL (Sigma) in a 24-well plate before transcription and treatment ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were plated in 6-well plates and stained/fixed with 2.5mg/mL solution of crystal-violet (Sigma, Cat #C3886) in 20% methanol.
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bioRxiv - Neuroscience 2021Quote: ... For analysis of human OL 3000-4000 cells were seeded on wells coated with 5-10 µg/ml mouse-laminin (#L2020, Sigma) and 0.025 % poly-L-lysine (#P4707 ...
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bioRxiv - Neuroscience 2020Quote: ... Anti-α-tubulin primary antibody as well as secondary antibodies coupled to horseradish peroxidase (HRP) were obtained from Sigma-Aldrich. AlexaFluor-labelled secondary antibodies were obtained from ThermoFisher ...
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bioRxiv - Neuroscience 2020Quote: ... and plated in 24-well plates containing 12-mm glass coverslips pretreated with 50 µg/ml poly-L-lysine (Sigma) in Borate buffer (1.24 g boric acid (Fisher) ...
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bioRxiv - Cancer Biology 2021Quote: 105 HCT116 cells as well as HCT116 cells with acquired oxaliplatin resistance and HCT116 cells with BOLD-100/KP1339 resistance were seeded (N = 4 for each respectively) into 12-well plate (StarLab) with 2 mL McCoy’s 5A medium (Sigma-Aldrich) containing 10% FCS (BioWest ...
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bioRxiv - Cancer Biology 2021Quote: HDMB03 cells (3×105 in 6-well plates) were treated with global de-methylating agent 5-AzaC (5 μM) (5-Aza-2-deoxycytidine; Sigma). Following 96 hours of incubation ...
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bioRxiv - Cell Biology 2021Quote: Hek293T cells were plated in 24-well plates on cover glass (Carl Roth) coated with poly-L- Lysine (Sigma-Aldrich). Cells were transfected with 125 ng of plasmid and total plasmid levels were adjusted to 500 ng with empty plasmid ...
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bioRxiv - Neuroscience 2020Quote: ... 35-050-061) and plated at a density of 5.0×104 cells per well on poly-D-Lysine (Sigma, P6407) coated 24 well glass-bottom plates (Fisher ...
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bioRxiv - Physiology 2020Quote: ... HMDC (passage 7-9) were seeded onto plastic 6-well fibronectin-coated (10 μg/ml in PBS, Sigma-Aldrich, UK) culture plates (described below ...
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bioRxiv - Cell Biology 2021Quote: ... 2×105 cells were seeded in 6-well tissue culture plates and incubated at 37°C for 24 hours before exposure to 4 mM hydroxyurea (HU; Sigma) in regular growth medium for 5 hours ...
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bioRxiv - Microbiology 2020Quote: ... resuspended to an OD600 of 1 and added to wells coated with 20 μg/ml of collagen I (Sigma-Aldrich). BSA-coated wells (50 μg/ml ...
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bioRxiv - Plant Biology 2020Quote: ... The coding sequence of PhS3-RNase (without signal peptide) as well as its six mutant forms described above were separately cloned into engineered pET-30a (Novagen) containing N-terminal SUMO tag to produce SUMO-His-tagged proteins ...
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bioRxiv - Microbiology 2020Quote: ... All purified proteins were analyzed by SDS-PAGE followed by Coomassie blue staining as well as by Western blot using a mouse monoclonal antibody against the 6xHis-Tag (Novagen). Protein aliquots were stored at −80⁰C in 50% glycerol (v/v ...
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bioRxiv - Systems Biology 2020Quote: ... paired SUSD2- and SUSD2+ cells were seeded 6-well plates at a density of 2×105 cells/well and decidualized when 90% confluent with 0.5 mM 8-bromoadenosine cAMP (8-bromo-cAMP; Sigma-Aldrich) and 1 µM medroxyprogesterone acetate (MPA ...
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bioRxiv - Microbiology 2020Quote: ... Wells were coated overnight with 1 μg of each ligand dissolved in bicarbonate buffer: collagen type I and IV (Sigma), elastin (Sigma) ...
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bioRxiv - Microbiology 2021Quote: GFP-LC3 HeLa cells or HeLa-Difluo hLC3 cells were seeded onto Nunc Lab-Tek Chamber Slide system 8 wells (Sigma). The day of the experiment ...
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bioRxiv - Immunology 2020Quote: ... at 1 million/mL concentration (200,000 cells/well) with 10ug/mL LPS and varied concentrations of (Z) 4-hydroxytamoxifen (Sigma-Aldrich). Analysis was performed by flow-cytometry at 48 ...
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bioRxiv - Neuroscience 2021Quote: ... Astrocyte cell line cultures were scraped off 6-well plates with ice-cold RIPA buffer with protease and phosphatase inhibitors (MSSAFE, Sigma) and transferred to a chilled microcentrifuge tube ...
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bioRxiv - Cancer Biology 2021Quote: ... were seeded in 6-well plates before addition of fresh medium containing viral supernatant (~5×105 transducing units) and Polybrene (8 μg/mL; Sigma) and ‘spin-oculated’ for 90 min at 1,500 rpm ...