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Citations for Millipore Sigma :
7301 - 7350 of 10000+ citations for QuantiChrom β N Acetylglucosaminidase Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... and N-(1-Naphthyl)ethylene diamine dihydrochloride (NEDC) (cat#222488-5G) and penicillin/streptomycin (PEN/Strep; cat# P0781) were from Sigma-Aldrich Chemie GmbH (Taufkirchen ...
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bioRxiv - Cancer Biology 2024Quote: Cells were seeded in a 96-well plate at a density of 15,000 cells per well in 50 µL of medium containing the HDAC substrate N-(4-Methyl-7-aminocoumarinyl)-Nα-(t-butoxycarbonyl)-Nω-acetyllysineamide (21 μM, MAL, Sigma-Aldrich) and incubated for 8 h at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... Expression of N-Flag WT or mutant TTC5 in rescue cell lines was detected by anti-Flag M2-HRP (Sigma A8592), monoclonal anti-Flag M2 (Sigma F3165) ...
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bioRxiv - Immunology 2024Quote: ... individual lung lobes were isolated and homogenized in 1 mL PBS and subsequently hydrolyzed through the addition of 1 mL of 12 N HCl (Sigma Aldrich) followed by incubation at 120°C for 18 hours ...
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bioRxiv - Immunology 2021Quote: ... Expression of PTSN was silenced in U2-OS and 293A cells by lentiviral transduction of a pLKO1 shRNA against both β- and α-PTSN (TRCN0000282572, Sigma-Aldrich). pLKO1 shRNA (SHC016V ...
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bioRxiv - Immunology 2021Quote: ... for 1-2 hours and then probed overnight at 4 °C with primary antibodies (all diluted 1:1000 unless noted otherwise): Mouse β-actin (Sigma; A-1798), pro and mature IL-1β (R&D Systems ...
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bioRxiv - Biochemistry 2019Quote: ... Them these membranes were subsequently incubated overnight for the western blotting using one of two types of antibodies: monoclonal anti-β-actin antibody (Sigma-Aldrich, USA) or a primary antibody ...
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bioRxiv - Cell Biology 2019Quote: Cold KIF3A purification buffer (50 mM NaPi, 400 mM KCl, 2 mM MgCl2 × 6 H2O, 0.001% Brij35, 0.75 mM β-mercaptoethanol, Sigma#M3148, 0.2 mM ATP) supplemented with protease inhibitors and DNaseI was added to frozen BL21-RIL cell pellets expressing a KIF3A construct ...
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bioRxiv - Bioengineering 2020Quote: ... Differentiated endodermal or neuronal cells were processed as described above and immunostained with anti-β-III-Tubulin (Sigma-Aldrich, Cat. # T8660; 1:100), anti-NESTIN (MerckMillipore ...
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bioRxiv - Bioengineering 2020Quote: ... shaken at 250 rpm at 37 °C until the OD600 value reached in the ranges of 0.5-0.8 and subsequently induced with isopropyl β-D-1-thiogalacto-pyranoside (IPTG; Sigma-Aldrich, St. Louis, MO) at a final concentration of 1 mM ...
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bioRxiv - Neuroscience 2020Quote: ... 6-month-old littermate matched or matched mice of the indicated genotypes were injected subcutaneously with vehicle [40% (w/v) (2-hydroxypropyl)-β-cyclodextrin (Sigma–Aldrich #332607)] or MLi-2 dissolved in vehicle at a 30 mg/kg final dose ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were harvested in ice and suspended in buffer (25 mM Tris pH 8.0, 200 mM NaCl, 10 mM imidazole, 10 % glycerol, 5 mM β-mercaptoethanol, and SIGMA protease inhibitor cocktail), treated with 25 μM PMSF immediately before lysis by sonication ...
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bioRxiv - Microbiology 2020Quote: ... expression was induced at 16°C and 250 rpm for 24 h via addition of 1 mM isopropylthio-β-D-galactoside (IPTG; Sigma-Aldrich, USA). Following induction ...
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bioRxiv - Neuroscience 2021Quote: ... for two hours prior to overnight incubation at room temperature with primary antibodies against dopamine-β-hydroxylase (DBH, a marker of noradrenergic neurons: Mouse, 1:500, Millipore, MAB308, UK), mCherry (Rabbit ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were incubated overnight at 4°C in primary antibodies (anti-lamin B1, ab16048, Abcam, 1:5000; anti-β-Actin, A1978, Sigma, 1:10,000) in 4% BSA/TBS + 0.025% sodium azide ...
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bioRxiv - Cancer Biology 2020Quote: ... Anti-β-actin antibody (AC-15; catalog #A5441) utilized for western blot (1:10,000) was obtained from Sigma-Aldrich (St. Louis, MO, USA). Alexa 568-conjugated secondary antibody (catalog# A11031 ...
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bioRxiv - Cell Biology 2021Quote: ... 63-83 day old mice of the indicated genotypes were injected subcutaneously with vehicle [40% (w/v) (2-hydroxypropyl)-β-cyclodextrin (Sigma–Aldrich #332607)] or MLi-2 dissolved in the vehicle at a 30 mg/kg final dose ...
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bioRxiv - Cancer Biology 2022Quote: ... were established by the Institute for Precision Medicine (Pittsburgh, PA) and maintained in media as detailed in (40) with 1nM β-estradiol (Sigma-Aldrich #E8875) supplementation ...
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bioRxiv - Cell Biology 2022Quote: ... The fixed cells were then washed twice with wash buffer plus 1.2 M sorbitol and digested with 10 µl of 20T 50 mg/ml zymolyase (Nacali Tesque, 07663-91) supplemented with 15 µl β- mercaptoethanol (Sigma-Aldrich, M3148) for 45 minutes at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... The optical density of bands corresponding to defined proteins was determined densitometrically with the BIO-1D software (Vilber Lourmat) and expressed in relation to β-actin used as a loading control (Sigma, A-3854).
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bioRxiv - Neuroscience 2021Quote: ... Double-stranded RNA expression was induced by the addition of 0.4 mM of isopropyl-β-D-thiogalactopyranoside (I6758, Sigma, St. Louis, MO, USA) during peak culture growth ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the culture medium was withdrawn and the cells were washed with PBS and suspended in 350 μL of Lysis Buffer (with 1% of β-mercaptoethanol, Sigma, MO, USA) for RNA isolation (AllPrep DNA/RNA/miRNA Universal Kit ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration was normalized for all tissues with RIPA buffer and 2x SDS sample buffer containing β-mercaptoethanol (Sigma-Aldrich, St. Louis, MO) was added ...
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bioRxiv - Neuroscience 2022Quote: ... for two hours prior to overnight incubation at room temperature with primary antibodies against dopamine-β-hydroxylase (DBH, a marker of noradrenergic neurons: Mouse, 1:500, Millipore, MAB308, UK), mCherry (Rabbit ...
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bioRxiv - Systems Biology 2019Quote: ... Inhibitors were added at the following concentrations: 40 μM 5,6-dichloro-l-β-D-ribofuranosyl benzimidazole (DRB) (D1916, Sigma-Aldrich, St. Louis, MO); 0.25 μM Flavopiridol (CS-0018 ...
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bioRxiv - Molecular Biology 2019Quote: ... JQ1 was delivered every other day via intraperitoneal injection at a concentration of 50mg/kg in a 1:4 DMSO:10% (2-Hydroxypropyl)-β-cyclodextrin (Sigma Aldrich; 389145) vehicle starting the day of TAC surgery ...
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bioRxiv - Biochemistry 2020Quote: ... the cells were collected after 24-hr transfection in detergent-free buffer (40 mM HEPES, pH8.0, 150 mM NaCl, 10 mM β-glycerophosphate, 10 mM sodium pyrophosphate, 2 mM EDTA, 1x Sigma protease inhibitor cocktail). The cells were lysed by sonication for 3 seconds on ice followed by ultracentrifugation at 90,000xg in a TLA100.3 rotor for 1 hour at 4°C ...
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bioRxiv - Immunology 2020Quote: Data was generated from immunizations of three juvenile New Zealand white rabbits: two rabbits with recombinant Escherichia coli Beta-gal (β-galactosidase from Sigma Aldrich, Inc.), and one rabbit with two linear peptides RNSEEARTDRPSQQLRSLNGE (peptide A ...
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bioRxiv - Genetics 2020Quote: ... Specific primers for mouse leptin and 2 mouse housekeeping genes used for normalization (β-actin and 34B4 mouse genes) were purchased from Sigma (Sigma, France). We used primers for Leptin (forward ...
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bioRxiv - Cancer Biology 2022Quote: ... 2938), pAktS473 (CST, 4060), tErk (CST, 4696), pErkT202/Y204 (CST, 4370), pS6S235/236 (CST, 4858) and β-actin (CST, 4970/ Sigma-Aldrich, A5441) antibodies were used to detect the individual proteins ...
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bioRxiv - Neuroscience 2022Quote: ... we used the CSF-1R inhibitor BLZ945 (kindly provided by Novartis, Basel, Switzerland) dissolved in 20% (2-hydroxypropyl)-β-cyclodextrin (Sigma-Aldrich, Germany). A dose of 200 mg/kg bodyweight was applied by oral gavage in adult (8-weeks old ...
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bioRxiv - Cancer Biology 2022Quote: ... mice were given once daily intraperitoneal injections of 50 mg/kg olaparib or vehicle control (10% 2-hydroxypropyl-β-cyclodextrin, Sigma-Aldrich #C0926) for 21 days ...
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bioRxiv - Bioengineering 2022Quote: ... To prevent cross-linking of newly synthesized elastic or collagen fibers, female and male WT (FWT, MWT) and female and male MFS (FMFS, MMFS) mice were given β-aminopropionitrile (BAPN; Sigma Aldrich A3134) ad libitum at 2000 mg/l in the drinking water for 4 weeks31 beginning either at 4 (P28 ...
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bioRxiv - Biochemistry 2022Quote: ... coated with human plasma fibronectin + 0.1% gelatin in DMEM low glucose + 7% (vol/vol) FBS + P/S + 10 μM cytosine-β-d-arabinoside (AraC, Sigma Aldrich, C1768-500MG). The mitotic inhibitor ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated overnight at 4 °C with blocking solution containing the primary antibodies rabbit anti-allograft inflammatory factor 1 (Iba1, dilution 1:200; #019-19741, Fujifilm Wako, Japan) and mouse anti-β-actin (dilution 1:400, Sigma-Aldrich #A3854). Cells were then incubated for 1 hour with the secondary antibodies AF488-conjugated anti-rabbit IgG (dilution 1:500 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... HEK293 cells stably expressing a tTA-dependent luciferase reporter and a β-arrestin-TEV protease fusion gene (kindly provided by Brian Roth) were maintained in DMEM (Sigma Aldrich, D6492), supplemented with 10% FBS (Sigma Aldrich ...
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bioRxiv - Plant Biology 2024Quote: ... Vacuoles were treated for 5 minutes on ice with either 10mM adenosine 5’-(β,γ-imido) triphosphate lithium salt hydrate (A2647; Sigma Aldrich), 15µM gramicidin (G5002 ...
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bioRxiv - Immunology 2024Quote: ... Bp was transferred to Stainer-Scholte medium supplemented with 1mg/ml (2,3,6-tri-O-methyl)-β-cyclodextrin Heptakis (Sigma-Aldrich, Cat. H0513-5G) and incubated in a rolling drum at 180 rpm at 37°C for 24 hours ...
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bioRxiv - Biochemistry 2024Quote: ... The solvent was evaporated under a stream of nitrogen before adding 5 ml of a 5 mM (6.55 mg/ml) solution of methyl-β-cyclodextrin (Sigma 332615, Mn∼1310) prepared in Buffer A ...
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bioRxiv - Cell Biology 2024Quote: The methods used are similar to those described in Shin et al.37 and Castellano et al.3 Cells were cholesterol-depleted using cell-culture media supplemented with methyl-β-cyclodextrin (MCD, Sigma-Aldrich, #C4555) and lipid-depleted serum (Lipoprotein Deficient Serum from fetal calf ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were treated with cholesterol (3β-hydroxy-5-cholestene, Sigma-Aldrich, C-3045; cholesterol–methyl-β-cyclodextrin, Sigma-Aldrich, C-4951, Missouri, USA), IL1β (GenScript ...
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bioRxiv - Cell Biology 2023Quote: ... 2011) were attached to the glass surface in each chamber via anti-β-tubulin antibodies (Sigma-Aldrich, T7816, 20 μg/ml in PBS). Subsequently ...
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bioRxiv - Cancer Biology 2023Quote: ... WB 1:1000), anti-ITGA5 (Cat. #: 4705, CST, WB 1:1000) and anti-β-actin (Cat. #: A2228, WB 1:10 000, Sigma-Aldrich Inc.). Appropriate secondary antibodies (IgG-Fc Specific-Peroxidase ...
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bioRxiv - Cell Biology 2023Quote: ... cells were cultured in osteogenic medium (α-MEM-complete, supplemented with 2 mM β- glycerophosphate (BGP) and 50 µg/ml L-ascorbic acid (both Sigma-Aldrich)) ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were washed with 0.05% Tween-20 in phosphate buffered saline (PBST) for 3 × 5 min with agitation and incubated with anti-β actin (1:1000 rabbit polyclonal (Sigma Aldrich; A2066) or 1:5000 mouse monoclonal (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... Antibodies used in this study include: Rb anti-Tg-β-tubulin (1:5,000 dilution) and mouse m2 anti-FLAG (Sigma; 1:1,000 dilution).
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bioRxiv - Biochemistry 2023Quote: ... 3-month-old littermate or age-matched mice of the indicated genotypes were injected subcutaneously with vehicle [40% (w/v) (2-hydroxypropyl)-β-cyclodextrin (Sigma–Aldrich #332607)] or MLi-2 dissolved in the vehicle at a 30 mg/kg final dose ...
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bioRxiv - Neuroscience 2024Quote: ... 24.75 µl of activity assay buffer (0.25% w/v taurocholic acid in citrate/phosphate buffer) containing 2 mM 4-methylumbelliferyl-β-D-glucopyranoside substrate (4MU-Gluc; Sigma-Aldrich, # M3633) in activity assay buffer ...
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bioRxiv - Biophysics 2021Quote: ... and streptavidin (used for the LNP detachment assay) were from Sigma-Aldrich. Citrate buffer (Teknova ...
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bioRxiv - Cell Biology 2020Quote: ... PBS was then removed and OPD assay solution (Cat # P9187, Sigma-Aldrich) was added for 30 minutes in the dark ...