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Citations for Millipore Sigma :
7201 - 7250 of 10000+ citations for QuantiChrom β N Acetylglucosaminidase Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... which were then derivatized by adding 100 μl 90% N,O-bis(trimethylsilyl)-trifluoroacetamide-10% tetramethylsilane (TMS) (Sigma, St. Louis, MO) and 200 μl anhydrous pyridine (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... EPR spin-trapping studies were performed using the spin-trapper 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) (Sigma, St. Louis, MO) at 10 mM ...
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bioRxiv - Cell Biology 2021Quote: ... permeabilized with 0.1% Triton X-100 in PBS for 15 min and inspected for sample quality control following Hoechst staining in imaging buffer (IB: 700 mM N-acetyl-cysteine (Sigma, A7250) in ddH2O ...
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bioRxiv - Cell Biology 2021Quote: ... Then cell lysates were subject to protein immunoprecipitations using indicated beads (For N-Flag-S-protein-tagged, FLAG M2 (Sigma-Aldrich) or S-protein beads (Millipore ...
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bioRxiv - Cell Biology 2021Quote: Labeling reagents XTT (sodium 3’-[1-(phenylaminocarbonyl)- 3,4-tetrazolium]-bis (4-methoxy6-nitro) benzene sulfonic acid hydrate) and PMS (N-methyl dibenzopyrazine methyl sulfate) were purchased from Sigma-Aldrich. Assay was performed according to protocol in Cell proliferation kit II (XTT ...
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bioRxiv - Cancer Biology 2022Quote: ... The solution was neutralized using sodium acetate pH 7.0 and DNA sample was transferred to Whatman Hybond N+ Blotting Membrane (Millipore Sigma, Z761079). Nucleic acids were cross-linked by to the membrane by baking at 60 °C for 30 min followed by UV exposure ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated o/n at 4°C with the following primary antibodies at a dilution of 1/1000: anti-LC3 (Sigma, L7543), anti-Atg5-12 (Abnova ...
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bioRxiv - Plant Biology 2022Quote: Proteasome activity was measured using the fluorogenic substrate N-Succinyl-Leu-Leu-Val-Tyr-7-Amido-4-Methylcoumarin (Millipore Sigma; S6510) as described Üstün et al ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed three times in DPBS and incubated in DMEM supplemented with 100 ng/mL anhydrotetracycline and 10 mM N-acetyl cysteine (NAC, Sigma-Aldrich) when indicated ...
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bioRxiv - Cell Biology 2022Quote: ... It was inserted with N and C-terminal tags (His6 and StrepII, respectively) in the baculovirus transfer vector pIEX/Bac3 (Merck Novagen #717263).
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bioRxiv - Immunology 2022Quote: ... and mesenteric) (n=3) were pooled in digestion medium consisting of RPMI 1640 with 2% fetal bovine serum (FBS) (Sigma-Aldrich), deoxyribonuclease (DNase ...
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bioRxiv - Bioengineering 2022Quote: Films (N = 5 per condition) were lyophilized and incubated for 48 h in 5 wt% trichloroacetic acid (TCA, T6399, Sigma-Aldrich). Scaffolds (N = 5 per condition ...
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bioRxiv - Microbiology 2022Quote: ... 3-(N-Morpholino)propanesulfonic acid (MOPS) or 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (HEPES) buffer (all purchased from Sigma-Aldrich, USA). Macrocolonies were excised and resuspended in 2 mL of sterile 1X PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Cysteine containing phosphopeptide was conjugated to carrier protein Limulus hemocyanin using hetero-bifunctional crosslinker m-maleimidobenzoyl-N-hydroxysulfosuccinimide ester (Sigma# 803227). This conjugate was used to immunize New Zealand white rabbits (Charles River Laboratories) ...
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bioRxiv - Bioengineering 2022Quote: ... The top flow channel was perfused with hECSR and the bottom chamber with N-Formylmethionyl-leucyl-phenylalanine (fMLP, 10 nM, Sigma Aldrich), a potent neutrophil chemoattractant ...
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bioRxiv - Cancer Biology 2022Quote: ... Actin (#A1978) and DCLK1 (targeted to the N-terminus, #62257) (#SAB4200186) were purchased from Sigma Aldrich (Sigma-Aldrich, St. Louis, MO). Bax (#5023) ...
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bioRxiv - Plant Biology 2021Quote: ... 5-DAG seedlings were treated with 0.8 M mannitol [32] with 0.1% (w/v) Calcofluor white (Sigma-Aldrich, cat. n. 18909) for 30 min ...
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bioRxiv - Immunology 2021Quote: ... CD4+ T cells from iCD4-PRMT5fl/fl and iCD4-PRMT5Δ/Δ mice (n = 3 pooled mice/sample and n = 3 samples/group) were lysed in our in-house lysis buffer (50 mM triethylammonium bicarbonate, Millipore Sigma, catalog no ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 3’-phosphoadenosine-5’-phosphosulfate (PAPS), and [1,2,6,7-3H(N)]dehydroepiandrosterone ([3H]DHEA, 60 Ci/ mmol) were purchased from Sigma-Aldrich (St. Louis,MO). SDS–polyacrylamide gel electrophoresis reagents were obtained from Bio-Rad (Hercules ...
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bioRxiv - Biochemistry 2021Quote: ... varieornatus CAHS1 protein was constructed and cloned as a fusion protein with a hexahistidine (His6) tag at the N-terminus using a pET28a vector (Novagen, Merck). CAHS1-N (Met1-Ser96 ...
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bioRxiv - Biochemistry 2021Quote: ... were constructed using standard genetic engineering techniques with a His6 tag at the N-terminus using the pET28a vector (Novagen, Merck). The recombinant CAHS1 protein ...
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bioRxiv - Neuroscience 2021Quote: ... catalog #96121229) were cultured as described (N. J. Ortner et al., 2020) in Dulbecco’s modified Eagle’s medium (DMEM; Sigma-Aldrich, catalog #D6546) that was supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2021Quote: U87 glioblastoma (ATCC HTB-14) and SK-N-BE(2) neuroblastoma (ATCC CRL-2271) were cultured in Dulbecco’s Modified Eagles Medium (DMEM) (Sigma-Aldrich, Australia) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2021Quote: Desiccated tissue sections mounted on ITO glass slides were sprayed using an HTX TM-Sprayer (HTX Technologies, LLC) with 10 mg/ml N-(1-Naphthyl) ethylenediamine dihydrochloride (NEDC, Sigma #222488) dissolved in 70%:30% methanol:water ...
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bioRxiv - Cell Biology 2022Quote: ... Pigmentation was prevented during experiments by treating the embryos with 0.002% N-phenylthiourea (PTU) (Sigma-Aldrich, St. Louis, US-MO, #P7629) from 1dpf onwards ...
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bioRxiv - Cancer Biology 2022Quote: ... Bioluminescent spectra was then acquired after VK3 and NAC (N-acetylcysteine) treatment or auranofin and buthionine sulfoximine (both Sigma, Burlington, MA) treatment or BBI-608 (Boston Biomedical ...
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bioRxiv - Plant Biology 2019Quote: ... Full-length GUN1 cDNA and N-terminal truncated versions as EcoRI-SalI PCR fragments were ligated into pET48b (Novagen, Wisconsin, USA) and transformed into E ...
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bioRxiv - Plant Biology 2019Quote: ... 5 mM EDTA, 150 mM NaCl, 2.5% SDS, 10 mM N-ethylmaleimide, 5 μl.ml−1 protease inhibitor [P9599, Sigma-Aldrich], 100mg PVPP). Samples were mixed gently for 10 minutes at room temperature and centrifuged at 16,000 xg for 1 minute ...
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bioRxiv - Synthetic Biology 2020Quote: ... magnesium sulfate (MgSO4), calcium chloride (CaCl2), 3-(N-morpholino)propane sulfonic acid (MOPS), and hydrogen peroxide (H2O2, 30%) were from Sigma Aldrich. N-3-oxo-dodecanoyl-L-Homoserine lactone (AHL ...
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bioRxiv - Biophysics 2019Quote: The tetra-p-tosylate salt of meso-tetrakis(4-N-methyl-pyridyl)porphine (TMPyP4+, Fig. 1) and potassium salt of poly(rA) from Sigma-Aldrich Chemical Co ...
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bioRxiv - Biochemistry 2019Quote: ... trimethylsilyl-N-methyl trifluoroacetamide (MSTFA) and N,O-Bis(trimethylsilyl)trifluoroacetamide (BSTFA) were obtained from CS-Chromatographie Service and pyridine from Sigma-Aldrich at >99.98% purity ...
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bioRxiv - Genomics 2019Quote: ... The dried extract was then resuspended in 5 μl of a hexane solution containing 7.5 ng/μl of n-dodecane (EMD Millipore Corp.) as an internal standard ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... directed against the N-terminal half of mouse AHR and the polyclonal goat anti-rabbit secondary antibody cross-linked to alkaline phosphatase (Sigma-Aldrich). The blot was developed using the AP Conjugate Substrate Kit (Bio-Rad).
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bioRxiv - Biophysics 2020Quote: ... 100 μL aliquots of 50 μM K311Dha in in 20 mM NaPi (pH 8) was reacted with 1000 molar equivalents of N-acetylcysteamine (Sigma Aldrich) for 12 hours at 37 °C and shaking at 500 rpm ...
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bioRxiv - Microbiology 2021Quote: ... cadavers or conidia were extracted in 2-mL vials with 500 µL n-hexane (for liquid chromatography LiChrosolv®, Sigma-Aldrich) for 5 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... 200 μL of the NaOH/DMSO slurry was added to the lyophilized N-glycans in addition to 100μL iodomethane (Sigma-Aldrich, #289566) and placed in on a vortex shaker for 20 minutes at room temperature with a microtube cap to prevent the lid from opening due to increased gas pressure ...
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bioRxiv - Microbiology 2020Quote: ... The fractions containing the complexes were incubated for 1 hr at 4°C with 10 mM N-hydroxysulfosuccinimide and 5 mM EDC (Sigma-Aldrich) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was discarded and the pellet resuspended in 1 ml of BHI containing 10 µM 5(6)-carboxyfluorescein diacetate N-succinimidyl ester (CFDA-SE, Sigma #21888). The suspension was incubated on a rotating shaker at 37°C and 200 rpm for 45 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then lysed under denaturing conditions using the lysis buffer (40 mM HEPES KOH, pH 7.4, 1% SDS) containing 5 mM N-ethylmaleimide (NEM, Sigma-Aldrich, E1275) to prevent deubiquitination and boiled for 5 min ...
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bioRxiv - Neuroscience 2020Quote: ... filled with pre-heated (to 37 °C) CUBIC-L solution (10% w/w N-butyldiethanolamine, 10%w/w triton X-100, in H2O, Sigma Aldrich) and kept at 37 °C under continuous shaking ...
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bioRxiv - Neuroscience 2021Quote: ... mediated knockdown of Zfp804a (the rat homolog of ZNF804A) in rat primary cortical neurons was conducted using the N-Ter Nanoparticle siRNA Transfection System (Sigma: N2788) at DIV15 per manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: NA activities of recombinant AIV strains were measured using the fluorogenic substrate MUNANA (2’-[4-Methylumbelliferyl]-alpha-D-N-acetylneuraminic acid; Biosynth and Sigma-Aldrich), as described previously [70] ...
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bioRxiv - Biophysics 2019Quote: ... then incubated for 1 h in a PBS solution of 1 mM methacrylic acid N-hydroxysuccinimide ester (MA-NHS, Sigma-Aldrich), diluted from a 1 M MA-NHS DMSO stock ...
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bioRxiv - Molecular Biology 2019Quote: Dry samples were resuspended in 300 µL 0.1 N HCl and filtered through 0.45 micron filters (EMD Millipore, Billerica, MA, USA). 5 µL of the filtered extracts was derivatized according to the AccQTag™ Ultra Derivitization Kit protocol (Waters Corporation ...
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bioRxiv - Microbiology 2019Quote: ... Cells were then resuspended in 10 ml of PBS supplemented with 2 mM DTSP (3,3′-Dithiodipropionic acid di(N-hydroxysuccinimide ester dissolved in DMSO, a crosslinking agent from Sigma, MO, USA) and incubated for 30 min ...
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bioRxiv - Developmental Biology 2021Quote: ... hearts were transferred in 50% glycerol for 1 h and then mounted in anti-fade mounting media solution containing 100% glycerol + 20%(w/v) n-propyl gallate (Sigma P3130).
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Cold exposure drives weight gain and adiposity following chronic suppression of brown adipose tissuebioRxiv - Physiology 2021Quote: ... clean up and trypsinisation was performed on 50-100 mg of frozen tissue (n=4/group) was homogenised in 500 μL CellLytic MT cell lysis buffer (Sigma, C3228) prior to removal of lipid and other contaminants using the ReadyPrep 2D cleanup Kit (Biorad ...
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bioRxiv - Physiology 2020Quote: ... before deproteinization with 1 N perchloric acid (final concentration 0.5 N) and the supernatant collected and filtered Rhrough a 0.22 μm filter (Millex-GV filter; Millipore Sigma, Burlington, MA). Filtered samples were separated using a 50 x 3 mm column (length x i.d ...
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bioRxiv - Physiology 2020Quote: ... physiological responses of the animals were analyzed with an intraperitoneal administration of 1 mg/kg of clozapine-n-oxide (CNO, Sigma-Aldrich) as described above with some modifications ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... was chopped into small pieces and transferred into a 15 mL plastic tube containing 10 mL of extraction solvent consisting of isopropanol (LC/MS grade, Carlo Erba) with N-decane (purity 99%, Sigma-Aldrich) (50 μg/mL ...