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Citations for Millipore Sigma :
7151 - 7200 of 10000+ citations for QuantiChrom β N Acetylglucosaminidase Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... The membranes that were loaded with fetal membrane and uterine tissue lysates were re-probed for 1 h at room temperature with a mouse anti-β-actin (ACTB) monoclonal antibody (Cat# A5441, Sigma-Aldrich). Then ...
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bioRxiv - Bioengineering 2024Quote: ... ODM was made using the basal medium supplemented with 10mM β-glycerophosphate disodium salt hydrate (BGP, sigma, G9422) and100 nM Dexamethasone (Dex, Sigma, D4902). For ECM production ...
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bioRxiv - Plant Biology 2023Quote: ... following Ghose (1987)) and Novozyme 188 (measured β-glucosidase activity of 15 units per gram liquid enzyme preparation, following Mielenz (2009) (Sigma-Aldrich). Sodium citrate buffer (50 mM ...
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bioRxiv - Cell Biology 2023Quote: ... cholesterol was delivered by incubating cells for 1 h with 100 μM of water-soluble methyl-β-cyclodextrin–cholesterol complex (Sigma-Aldrich) in DMEM/F-12 ...
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Optimization of highly inclined Illumination for diffraction-limited and super-resolution microscopybioRxiv - Biophysics 2023Quote: ... cells were washed once with PBS before being mounted on an imaging chamber and immersed in 500 μl of Imaging Buffer (IB: 100 mM β-MercaptoEthylamine hydrochloride (MEA, Sigma-Aldrich), 3% (v/v ...
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bioRxiv - Biophysics 2022Quote: ... Staining is performed overnight at 4 °C for α- and β-tubulin using a mixture of three primary antibodies (T5168, T6199, T5923, Sigma) at a combined dilution of 1:150 in blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... were transferred to 35 mm diameter petri dishes containing 3 ml of liquid GM supplemented with 10 μM estradiol (β-Estradiol, E8875, Sigma Aldrich, freshly prepared by diluting a 1 mM stock solution in DMSO ...
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bioRxiv - Microbiology 2023Quote: ... cultures were grown in 4% w/v clarified V8 broth supplemented with 30 µg/ml β-sitosterol (Sigma-Aldrich, St. Louis, Missouri) and incubated in the dark at 22 °C for 4 to 6 weeks ...
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bioRxiv - Immunology 2023Quote: ... was purchased from Enzo Life Sciences and mouse monoclonal anti-β-actin (A5441), along with anti-mouse and anti-rabbit antibodies coupled with HRP (A4416, A6154) were obtained from Sigma-Aldrich.
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bioRxiv - Cell Biology 2023Quote: ... was cultured in bisulfite/sulfite-free Brucella (BSFB) broth (4 mL, 0.5% NaCl, 0.2% β-cyclodextrin, 0.1% dextrose (all from Sigma-Aldrich, St. Louis, MO), and ...
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bioRxiv - Cell Biology 2023Quote: ... followed by growing them in LB media at 37°C and inducing them by 1 mM isopropyl β-D-thiogalactopyranoside (IPTG, Sigma-Aldrich) overnight at 25°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were starved for 24 h in DMEM without FBS and cell proliferation was inhibited by treating the cells with 10 μM Cytosine β-D-Arabinofuranoside (AraC, C1768, Sigma-Aldrich) for 2 h ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated overnight at 4°C with the corresponding primary antibodies diluted in blocking buffer: mouse anti-β-Actin 1:2000 (Sigma, #A544); mouse anti-3R-Tau 1:1000 ...
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Understanding Campylobacter coli isolates from the Vietnamese meat production network; a pilot studybioRxiv - Microbiology 2023Quote: ... supplemented with 2.5% defibrinated horse blood (Oxoid, UK) along with 20 mg/L β- Nicotinamide adenine dinucleotide sodium salt (Sigma Aldrich, UK), at 120 revolutions per minute (RPM) ...
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bioRxiv - Microbiology 2023Quote: ... The viral pellet was then lysed in Western blot lysis buffer with 4x Protein Sample Loading Buffer (LICOR) supplemented with 10% β-mercaptoethanol (Sigma Aldrich) and heated at 95°C for 5 min.
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A synthetic elastic protein as molecular prosthetic candidate to strengthen vascular wall elasticitybioRxiv - Cell Biology 2023Quote: ... The culture was diluted 1:100 and SEP expression was induced at the exponential growth phase by the addition of 1 mM isopropyl-β-D-thiogalactopyranoside (IPTG) (Sigma–Aldrich) for 4h at 30°C under constant stirring ...
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bioRxiv - Cell Biology 2023Quote: ... mouse monoclonal anti-αSMA (Cat: A5228) and mouse monoclonal anti-β-actin (AC-74) (A2228) antibodies were from Sigma (St Louis, MO). Anti-TGFβR1 (Cat ...
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bioRxiv - Microbiology 2023Quote: ... Spectinomycin resistant transformants were transformed with a plasmid carrying a FLP recombinase which was induced by 1 mM isopropyl-β-D-thiogalactopyranoside (IPTG) (Fischer BioReagents, 367-93-1) and 1.5 mM theophylline (Sigma, T1633-100G), allowing for the removal of the spectinomycin resistance cassette via recombination ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 x 106 cells of each line were washed in PBS and then resuspended in 350 μL lysis solution (supplemented with 1% β-mercaptoethanol (Sigma-Aldrich)) ...
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bioRxiv - Biochemistry 2024Quote: ... The membrane was then washed with TBS-T and incubated with 1:10000 goat anti-rabbit or rabbit anti-mouse poly-HRP secondary antibody (Thermo, 32260) and 1:5000 anti-β-actin-peroxidase antibody (Sigma, A3854) in TBS-T for 1 hr ...
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bioRxiv - Neuroscience 2024Quote: ... Incubation with the primary antibodies (anti-aSyn Syn-1 mouse, BD Transduction Laboratories 1:1000; anti-pS129-α-syn Rabbit Abcam Ab51253; mouse anti-β-actin, 1:10.000, Sigma Aldrich) was performed overnight at 4 °C in 5% Albumin Bovine Fraction V (BSA)/TBS-Tween ...
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bioRxiv - Immunology 2024Quote: ... These diluted test peptides were co-incubated with 0.1 μM biotin-labeled peptide and 50 nM purified MHC-II proteins (ProImmune, Oxford, United Kingdom) at 37°C for 24h in the presence of Octyl-β-D-glucopyranaside (Sigma-Aldrich). Then ...
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bioRxiv - Immunology 2024Quote: ... Antibodies used in Western blot were (listed in Appendix Table S4): mouse monoclonal antibodies anti-β-actin and anti-UVRAG (both from Sigma-Aldrich), anti-LC3B (NanoTools) ...
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bioRxiv - Plant Biology 2024Quote: ... Homozygous ESR2:ER AHP6::GFP and ESR2:ER IPT5::GUS seedlings were transplanted to MS medium with 10 μM β- estradiol (Sigma–Aldrich). Treated seedlings were removed from the plates and processed for expression visualization 48 hours after ESR2 induction ...
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bioRxiv - Plant Biology 2020Quote: ... 150 uM stock trans-zeatin in .01 N KOH was added to autoclaved media to create 150 nM working concentration (Sigma, Z0876). For the NPA experiment ...
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bioRxiv - Biophysics 2021Quote: ... Cells were then collected and re-plated at 500 cells/well with either 5 μM 4-nitroquinolone N-oxide (4NQO) (Sigma, N8141) or a dimethyl sulfoxide (DMSO ...
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bioRxiv - Cancer Biology 2021Quote: ... cantilever B with nominal spring constant of 0.02 N/m) (Bruker, USA) with a 25 µm diameter polystyrene microsphere (Sigma Aldrich, USA) glued to the free end were used in all AFM measurements ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then incubated overnight at 4°C in a wet chamber with the following antibodies: anti-P2X7 (cat. n. P8232, Sigma-Aldrich) and anti-Tom 20 (cat n ...
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bioRxiv - Cell Biology 2020Quote: ... Carbonyl cyanide α-[3-(2-benzothiazolyl)6-[2-[2-[bis(carboxymethyl)amino]-5-methylphenoxy]-2-oxo-2H-1-benzopyran-7yl]-b-(carboxymethyl)-tetrapotassium salt (FCCP, cat. n. C2920, Sigma-Aldrich) was solubilized in ethanol to a final stock concentration of 10 mM ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Steidle and Dettner, 1993) in 70 μl hexane (company, GC/MS analytical grade) containing 150 ng/μl n-octadecane (Sigma Aldrich) as an internal standard ...
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bioRxiv - Molecular Biology 2020Quote: ... and mice exposed to 5 days of photo-oxidative damage (PD) (n=6) were lysed in CellLyticTM Cell Lysis Buffer (Sigma-Aldrich) supplemented with a protease inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Genomics 2020Quote: ... Subsequently sections were incubated for 1h at RT in PBS blocking buffer containing 5% horse serum (Sigma Aldrich, Cat. N: H0146), 0.3% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Neuroscience 2019Quote: ... and spinal cords of both sexes (n = 3 each) were fixed in 4% PFA and embedded in 5% agarose (Type IX-A; Sigma-Aldrich) supplemented with 20% sucrose ...
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bioRxiv - Immunology 2020Quote: ... The bioswitch labeling procedure was performed according to the protocols described in (11, 45) with modifications using N-ethylmaleimide (NEM, Sigma Aldrich) to alkylate the reduced thiols ...
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bioRxiv - Evolutionary Biology 2019Quote: ... we treated females in the Pregnancy GCs (n = 44 total litters from 43 unique females) and Lactation GCs (n = 18 litters from 17 unique females) treatment groups with exogenous cortisol (hydrocortisone, Sigma H4001) dissolved in peanut butter and wheat germ mixture (8 g of peanut butter ...
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bioRxiv - Microbiology 2019Quote: ... cells were transferred (2% inoculation) into 50 ml of T-R minimal medium supplemented with 0.5% (w/v) N-acetylglucosamine (Sigma-Aldrich #G4875) as a carbon source and incubated ~12 hr at room temperature and 150 rpm ...
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bioRxiv - Developmental Biology 2019Quote: ... Retinal explants were grown as for DRGs but without NGF and with medium supplemented with N-2 (Sigma-Aldrich, 100x concentrate) and bovine pituitary extract (200μg/ml ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Peaks were tentatively identified by their mass spectra and retention time index based on a C21-C40 n-alkane standard solution (Sigma-Aldrich).
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bioRxiv - Neuroscience 2019Quote: ... whole brains of females (n = 6) were fixed in 4% PFA and embedded in 5% agarose (Type IX-A; Sigma-Aldrich) supplemented with 20% sucrose ...
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bioRxiv - Molecular Biology 2019Quote: ... tendon fascicles (n=6, independent samples from different mice) were stained with 2’,7’-Dichlorofluorescin diacetate (DCFH-DA, D6883, Sigma Aldrich). Control fascicles and cultured fascicles were incubated for 30 minutes with 50μM DCFH-DA in PBS at either 29°C ...
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bioRxiv - Microbiology 2021Quote: ... a blocking step of incubation with sheep serum for 1 hour was followed by immunostaining for N using a mouse monoclonal antibody (αN009 48) and detected using a sheep anti-mouse FITC conjugate (Sigma, UK). Phalloidin Alexa Fluor 568 (Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: ... The MCF-7 and the SK-N-SH cells were cultured in RPMI 1640 and in Minimum Essential Medium Eagle (MEM) from Sigma Aldrich, respectively ...
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bioRxiv - Biochemistry 2020Quote: We fused FtsE at its N-terminus to a 6xhis-followed by a TEV protease cleavage site in vector pETDuet™-1 (Novagen) that allows tunable expression of the fusion protein from a T7 promoter induced by isopropyl-β-D-1-thiogalactopyranoside (IPTG) ...
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bioRxiv - Biochemistry 2021Quote: ... N-containing fractions were concentrated on the Amicon Ultra-4 Centrifugal Filter Unit (Merck/Millipore; molecular weight cut-off 30.0 kDa) and loaded on two connected in series 1 ml Heparin-Sepharose columns (Cytiva ...
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bioRxiv - Biochemistry 2021Quote: ... N-containing fractions were concentrated on the Amicon Ultra-4 Centrifugal Filter Unit (Merck/Millipore; molecular weight cut-off 30.0 kDa) and loaded on a Superdex 200 10/300 GL size exclusion column (GE Healthcare Life Sciences ...
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bioRxiv - Neuroscience 2020Quote: ... myotube compartments were treated with 19 μM of the AChR competitive antagonist tubocurarine hydrochloride pentahydrate (DTC) (Sigma, Cat N° T2379-100G) 10 min before analysis ...
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bioRxiv - Neuroscience 2021Quote: ... Specimens were incubated overnight at 4°C in 1:5000 primary antibody (monoclonal anti-alpha-tubulin; Sigma Aldrich, cat n. T5168) diluted in BSA 1% + sheep serum 2% in PBT ...
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bioRxiv - Biophysics 2020Quote: ... The coating was done in the following three step procedure: I) 1mL of the resulting solution was added to 11.5 mg NHS (N-Hydroxysuccinimide, Sigma-Aldrich, Germany) and vortexed ...
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bioRxiv - Neuroscience 2020Quote: ... N-(Piperidin-1-yl)-5-(4-iodophenyl)-1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide (AM251, 0.3 µg/0.5 µL/hemisphere; n = 9; Sigma Aldrich, St. Louis, MO), or vehicle (VEH ...
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bioRxiv - Neuroscience 2020Quote: ... Dimethyl sulfoxide (DMSO) and clozapine-N-oxide (CNO; agonist at hM3Dq receptors) were purchased from Sigma-Aldrich (St. Louis, MO, USA). A stock solution of CNO (0.1 mg/ml in saline solution containing 0.5% DMSO was prepared and stored in aliquots ...