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Citations for Millipore Sigma :
7401 - 7450 of 10000+ citations for QuantiChrom β N Acetylglucosaminidase Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: Lactate quantification assay was performed following the manufacturer protocol (MAK017, Sigma Aldrich). Briefly ...
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bioRxiv - Systems Biology 2022Quote: ... qPCR assays were performed using FastStart Universal SYBR Green Master mix (Sigma) according to the manufacturer’s protocol with minor modifications ...
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bioRxiv - Neuroscience 2022Quote: ... and the protein concentration determined with a BCA assay (Sigma, Deisenhofen, Germany). Equal amounts of protein samples were separated on 8% Tris/glycine gels and then transferred to nitrocellulose membranes (GE Healthcare ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein concentration was measured using Bradford protein assay (Sigma, Missouri, USA). The total protein extract was separated via SDS-PAGE and then transferred onto the 0.22 µm or 0.45 µm PVDF membrane (Millipore ...
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bioRxiv - Molecular Biology 2022Quote: ... hydroxyurea (HU, Sigma-Aldrich, 4 mM and 1 mM in survival assay), formaldehyde (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: Proximity ligation assay was performed according to the manufacturer’s protocol (Sigma Aldrich). Briefly ...
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bioRxiv - Physiology 2022Quote: Total iron levels were measured by a colorimetric assay (MAK025-1KT, Sigma) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... Cells were stained using the AldeRed ALDH detection assay (Sigma/Merck, SRC150) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies used for immunofluroscence assay include MAP2 (1:500, Millipore, Ab5622); NeuN (1:200 ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was determined by BCA assay (Sigma Aldrich, cat no: B9643). 30-40 μg protein was loaded in Mini-PROTEAN® Tetra Cell Systems and TGX™ precast gels ...
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bioRxiv - Cancer Biology 2023Quote: ... and lysed in radioimmunoprecipitation assay buffer (RIPA) lysis buffer (Sigma Aldrich; R0278) freshly supplemented with protease and phosphate inhibitors (Cell Signaling Technology ...
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bioRxiv - Microbiology 2023Quote: ... The modulators included in this assay were oligomycin (1.5 μM, Sigma, #75351), Carbonyl cyanide-4 (trifluoromethoxy ...
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bioRxiv - Molecular Biology 2023Quote: ... resuspended in radioimmunoprecipitation assay (RIPA) buffer (Sigma-Aldrich, St. Louis, Missouri, USA) or non-reducing lysis buffer (NRL buffer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Assays were performed in 0.1 M phosphate buffer (Sigma-Aldrich, pH 7.2), supplemented with 50 mM NaHCO3 (Sigma- Aldrich).
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bioRxiv - Biochemistry 2023Quote: ... All other chemicals used for kinetic assays were ordered from Sigma-Aldrich.
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bioRxiv - Immunology 2023Quote: ... resuspended in a solution of Radio- immunoprecipitation assay (RIPA) buffer (Merck Millipore) containing protease inhibitor cocktail (Roche ...
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bioRxiv - Physiology 2023Quote: ... Protein concentration was qualified by Bradford assays (Sigma-Aldrich, B6916-500 mL). 30 μg soluble proteins were resolved on a 15% sodium dodecyl sulphate poly acrylamide gel via electrophoresis (SDS-PAGE ...
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bioRxiv - Biophysics 2023Quote: ... Protein concentration was measured using a Bradford assay (Sigma France, Lezennes, France). For SDS-PAGE analysis of purified proteins ...
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bioRxiv - Neuroscience 2023Quote: ... samples were homogenized in radioimmunoprecipitation assay (RIPA, by Sigma, St. Louis, MO) buffer (750 µL for brainstem and 500 µL for cerebellum ...
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bioRxiv - Bioengineering 2023Quote: Cell viability of various materials were determined using CCK-8 assay (Sigma Cell Counting Kit-8 ...
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bioRxiv - Biochemistry 2023Quote: ... Unbuffered DMEM assay medium is composed of DMEM (Sigma #5030; pH 7.4) supplemented with 31.6 mM NaCl ...
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bioRxiv - Cancer Biology 2022Quote: ... The protein concentration was determined using Bradford protein assay (Sigma, Missouri, USA). The total protein was then resolved on SDS-PAGE (8-12% ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined using the Bicinchoninic acid assay (BCA, Sigma-Aldrich). Proteins were then denaturated with Laemmli Buffer (Tris pH 6.8 (200mM) ...
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bioRxiv - Immunology 2023Quote: PLA assay was performed following the manufacturer’s protocol (Duolink®PLA, Sigma). The Treg cells were fixed with 4% paraformaldehyde for 15 min at RT and then plated on Poly-L-lysine coated (10 μg/ml ...
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bioRxiv - Developmental Biology 2023Quote: ... Proximity Ligation assay was performed according to manufacturer’s protocol (Sigma, cat# DUO96000). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... using the Duolink® Proximity Ligation Assay from Sigma (DUO92006, DUO92014, DUO92002) or the Proximity Ligation kit from Navinci (Cambridge bioscience ...
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bioRxiv - Physiology 2023Quote: ... Final protein concentrations were calculated by Bradford assay (Sigma-Aldrich Canada, Ltd.,) according to manufacturer’s guidelines with bovine serum albumin (BioRad Laboratories ...
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bioRxiv - Cancer Biology 2023Quote: ... A peptide challenging assay was conducted using PrEst Antigen ONECUT2 (Sigma APrEST86051) with a 5:1 ratio of peptide to ONECUT2 antibody ...
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bioRxiv - Genetics 2023Quote: ... Protein lysate was prepared in Radio-immunoprecipitation assay (RIPA) buffer (Merck Millipore) with a protease inhibitor cocktail (Roche ...
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bioRxiv - Plant Biology 2024Quote: ... After determining the protein concentration with Bradford protein assay reagents (Sigma B6916), the protein extract at 5 mg mL-1 was split into two LoBind 1.5 mL tubes and incubated with 100 µM DAPIA or equal volume of DMSO (1% v/v ...
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bioRxiv - Immunology 2023Quote: ... Tetrahydrobiopterin (H4B) needed for NOS activity assays were also obtained from Sigma. 16-HBE cells were purchased from American Type Culture Collection (ATCC ...
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bioRxiv - Microbiology 2024Quote: ... were lysed using 50μl of RadioImmunoPrecipitation Assay (RIPA) buffer (Millipore # 20-188) supplemented with protease and phosphatase inhibitors (Selleck # B15001 & B14001) ...
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bioRxiv - Immunology 2024Quote: ... and (2) the Human Cytokine 23-Plex Discovery Assay® (HD23; Millipore MILLIPLEX® Human Cytokine/Chemokine Magnetic Bead Panel II Immunology Multiplex Assay ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability testing was performed using the crystal violet staining assay (Sigma). After crystal violet was dissolved in 2% SDS ...
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bioRxiv - Biophysics 2024Quote: ... Adipogenic differentiation was evaluated using an oil red O assay (Sigma, O0625) following the manufacturers’ instructions.
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentration in each sample was measured using BCA assay (Sigma-Aldrich). 30µg of each sample were then prepared using the iST-NHS kit (Preomics) ...
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bioRxiv - Cancer Biology 2024Quote: ... cell viability was measured using the wst-1 assay (CELLPRO-RO, Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2024Quote: ... Translation assay was repeated by adding 20 µM of NITD008 (Sigma-Aldrich) in cell media for 2h before infection and during the course of infection.
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bioRxiv - Microbiology 2024Quote: ... the ammonia detection assay was completed as per manufacturer instructions (Sigma-Aldrich).
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bioRxiv - Microbiology 2024Quote: A turbidimetric assay similar to the protocol developed by Sigma-Aldrich (84) was used to measure HA digestion by recombinant HylB ...
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bioRxiv - Cell Biology 2020Quote: ... 4-well LabTek dishes were incubated 30 min at 37°C with 10 μg/ml fibronectin or O/N at 37°C with 20 μg/ml laminin (Sigma, L2020-1MG). RPE1 cells were then plated at low density in the dishes and imaged the next day ...
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bioRxiv - Genetics 2021Quote: ... CHCs were extracted by submersing individual wasps for 10 min in 10 µl n-hexane (≥ 99.0%; Sigma-Aldrich, St. Louis, MO, USA) using 1-ml glass vials equipped with a 0.1-ml micro insert (Alltech ...
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bioRxiv - Developmental Biology 2021Quote: ... vehicle control and treated (n=3 per group) were incubated in the sepeate fish tank containing dissolved BrdU (25 μg/L) (Sigma-Aldrich, USA) for 24 hours before tissue collection ...
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: Fixed hUC-MSCs were incubated with antibodies against some cytoskeletal proteins such as vimentin (1:50 in PBS; o/n at 4°C + 2 hr at 37°C) (Sigma; V6630, USA), pericentrin (1:1000 in PBS ...
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bioRxiv - Genomics 2019Quote: ... engrafted colonic tissues were finely minced with a razor then transferred to a Dounce tissue homogenizer (Kimble Chase KT885300-0002) in 2 mL of ice-cold Nuclei EZ Lysis buffer (Sigma #N-3408) supplemented with protease inhibitor (Roche #5892791001 ...
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bioRxiv - Physiology 2021Quote: ... Each mouse kidney was minced and homogenized using Dounce homogenizer (5x loose, 10x tight, Kimble Chase KT885300-0002) in 2 mL of Nuclei EZ lysis buffer (Sigma N-3408) supplemented with SuperaseIn and cOmplete ULTRA Tablets Mini (Roche ...
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bioRxiv - Immunology 2021Quote: Degranulation and cytokine production of NK cell subsets were determined after cytokine stimulation o/n with IL-12 (10ng/ml, Sigma Aldrich, USA) and IL-18 (5ng/ml ...
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bioRxiv - Synthetic Biology 2021Quote: ... rhaeticus cultures were grown shaking to mid exponential phase. Cells treated with 50 nM (E. coli) or 50 μM (K. rhaeticus) of AHL (N-Acyl homoserine lactone, Sigma-Aldridge K3255) and induced for approximately 4 doubling times (i.e ...
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bioRxiv - Bioengineering 2020Quote: ... 9µL antibody solutions in phosphate buffered saline (PBS) at 1-2 mg/ml were combined with 1 µL 2mM dibenzocyclooctyne-PEG4-N-hydroxysuccinimidyl ester (Sigma, Catalog # 764019) in dimethylsulfoxide (DMSO ...
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bioRxiv - Immunology 2021Quote: ... subsequently lysed and postnuclear supernatants were incubated o/n with monoclonal mouse anti-HA-antibody coupled to agarose beads (clone HA-7, Sigma-Aldich, USA) at 4°C ...