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Citations for Millipore Sigma :
7501 - 7550 of 10000+ citations for QuantiChrom β N Acetylglucosaminidase Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... Protein expression was induced with 0.5 mM isopropyl β-D-thiogalactopyranoside (IPTG; Carl Roth GmbH, Karlsruhe, Germany) and 5 µM all-trans retinal (ATR; Sigma-Aldrich, St. Louis, USA) was added ...
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bioRxiv - Neuroscience 2022Quote: ... then overnight in blocking solution with the following primary antibody: 1:1000 mouse anti S-100 β subunit (S2532, Sigma-Aldrich, St. Louis, MO). Sections were washed and then incubated for 2h in blocking solution with the following secondary antibody at a 1:500 dilution ...
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bioRxiv - Microbiology 2022Quote: ... Viral particles were resuspended in phosphate-buffered saline (PBS) and inactivated with 50 μl per mg of particles of a 1:100 dilution of β-propiolactone (BPL, Millipore Sigma, Cat# P5648) in cold water ...
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bioRxiv - Developmental Biology 2019Quote: ... Membranes were blocked in 5% dry milk powder in PBS with 0.1% Tween and incubated with anti-N1ICD (Val1744, CST, 1:1000), anti-Notch1 (D1E11, CST, 1:1000) and anti-β-actin (Sigma-Aldrich, 1:4000) overnight at 4°C ...
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bioRxiv - Bioengineering 2019Quote: ... or osteogenic differentiation medium [10,19] (OM: CM supplemented with 50 µg/mL ascorbic acid, 10 mM β–glycerophosphate, 0.1 µM dexamethasone; all additives from Sigma-Aldrich, St. Louis, MO) was added to each construct ...
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bioRxiv - Cell Biology 2021Quote: ... The Cdh1fl/fl(Foxj1-CreERT2) mice were further given stepwise increases (0, 3 & 30 mg/mL) of Acetyl- β- methylcholine chloride (Sigma Aldrich, MO, USA), to assess airway responsiveness.
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bioRxiv - Neuroscience 2021Quote: ... Carefully the gel was removed and transferred into a beaker filled with transfer buffer (25mM Tris, 192mM glycine, 0.1% SDS, and 10mM β-mercaptoethanol, all chemicals were purchased from Sigma Aldrich (St. Louis, MO)) and incubated briefly at 90°C and then transferred to a Trans-Blot Turbo mini nitrocellulose membrane using Trans-Blot Turbo Transfer system (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... Viral particles were resuspended in phosphate-buffered saline (PBS) and inactivated with 50 μl per mg of particles of a 1:100 dilution of β-propiolactone (BPL, Millipore Sigma, Cat# P5648) in cold water ...
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bioRxiv - Microbiology 2021Quote: ... Specificity of NamZ was further examined by testing para-nitrophenyl 2-acetamido-2-deoxy-β-D-glucopyranoside (pNP-GlcNAc) (Sigma-Aldrich, St. Louis, USA) and o-nitrophenyl β-D-galactopyranoside (oNP-Galactose ...
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bioRxiv - Microbiology 2022Quote: ... Bp strains were grown in Stainer-Scholte (SS) broth supplemented with 0.1 mg/ml of heptakis (2,6-di-O-methyl-β-cyclodextrin, Sigma Aldrich, St. Louis, MO, USA) [20 ...
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bioRxiv - Microbiology 2022Quote: ... Neurons were stained with the following primary antibodies overnight at 4°C: mouse monoclonal anti-β-III-Tubulin (Tuj1, 1:1000; Millipore cat. No. MAB5564) and rabbit polyclonal anti-SARS-CoV-2 (COVID-19 ...
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bioRxiv - Microbiology 2022Quote: ... EEA was measured via artificial substrates: Glucosidase and phosphatase using 4-methylumbelliferyl (MUF)-substrates (MUF-β-D-glucopyranoside and MUF-phosphate, respectively (Sigma-Aldrich, Darmstadt, Germany)) and leucine-aminopeptidase using a 7-amino-4-methylcoumarin (AMC)-substrate (L-leucine-AMC ...
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bioRxiv - Molecular Biology 2022Quote: ... Sections were then incubated with the following primary antibodies diluted 1:200 in PBS overnight at 4°C: β-spectrin non-erythrocyte (hpa012685, Sigma, St. Louis, MO), collagen type II (ab34712 ...
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bioRxiv - Cancer Biology 2024Quote: ... then blocked in either PBST-milk 5% (anti-ANP32E Abcam ab5993, anti-FLAG Sigma Aldrich F1804, anti-V5 Cell-Signaling 13202; anti-H3 Cell-Signaling 13202 4499, anti-β-actin Sigma-Aldrich A5441) or PBST-BSA 5% (anti-H2A.Z Abcam ab4174) ...
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bioRxiv - Cancer Biology 2024Quote: ... Myc (CST 71D10, 1:1000), p-EGFR Y1068 (CST 3777S, 1:1000), EGFR (CST 4267, 1:1000) and β-actin (Sigma A1978, 1:10000). Densitometry analysis was performed with ImageJ software (NIH).
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bioRxiv - Cancer Biology 2023Quote: ... Osteoblast positive control media (OB PC media; Supplemental Table 1) consisted of standard media supplemented with 10mM β-glycerophosphate (Sigma Catalog # G9422; Lot # SLBN8374V) and 50 ug/ml ascorbic acid (Sigma Catalog # A4403 ...
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bioRxiv - Biochemistry 2022Quote: ... and the cells were grown at 37 °C in LB medium with 50 μg/mL kanamycin to an OD600 of 0.8 and then induced with 1 mM isopropyl-β-d-thiogalactopyranoside (IPTG; Sigma-Aldrich, St. Louis, MO) at 30 °C for 5 h ...
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bioRxiv - Neuroscience 2022Quote: ... The membranes were blocked for 1 h in Tris buffered saline with Tween 20 (TBST) buffer with 5% bovine serum albumin (BSA) and incubated with primary rabbit anti-β-actin antibody (Millipore Sigma, cat. #SAB5600204) at 1:20000 ...
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bioRxiv - Biochemistry 2023Quote: ... USA), anti-PKM2-pS37 (SAB, 11456, Greenbelt, Maryland, USA), anti-ULK1 (CST, 8054, Danvers, Massachusetts, USA) and anti-β-actin (Sigma, A5441, Darmstadt, Germany). Anti-PKM2-pS333 antibodies targeting MLEpSMIK were manufactured by Dia-An Biotech ...
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bioRxiv - Physiology 2023Quote: The pancreatic β cell line INS-1E was cultured in a humidified chamber with 5% CO2 in RPMI-1640 medium (Merck, Sigma Aldrish, Darmstadt-Germany) at 11.1 mM glucose supplemented with 5% (vol./vol.) ...
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bioRxiv - Developmental Biology 2023Quote: ... The signal was compared to antibody stainings of loading controls (H3 and β-Catenin) and the membranes were quality controlled by Ponceau (Sigma Aldrich, #P7170-1L) staining ...
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bioRxiv - Physiology 2024Quote: ... Biotinylated proteins were eluted by incubation with 2x SDS-PAGE sample buffer containing β-mercaptoethanol (100 µmol/L, Cat. # M6250; Sigma-Aldrich, Darmstadt, Germany) for 20 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... The BC assay solution (2x) contains 0.4 M triethanolamine-HCl/Tris-HCl (Sigma), pH 8.0 ...
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bioRxiv - Developmental Biology 2021Quote: The primary antibodies used in this assay are: anti-B-actin (A5441, Sigma), 1:1000 ...
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bioRxiv - Molecular Biology 2019Quote: ... and immunostained using a standard sandwich assay with anti-Brn3a antibodies (Millipore, MAB1595) and secondary AlexaFluor 555 Anti-mouse (Invitrogen ...
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bioRxiv - Evolutionary Biology 2019Quote: ... we included an assay with porcine Na+/K+-ATPase (Sigma, Germany, A7510-5UN) as an internal standard ...
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bioRxiv - Neuroscience 2019Quote: ... 1ml of ice-cold Radio-Immunoprecipitation Assay (RIPA) buffer (Sigma-Aldrich, Dorset, UK) with added protease inhibitors (10%v/v)(Thermo Scientific ...
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bioRxiv - Immunology 2019Quote: ... The assay was performed in RPMI without phenol red and carbonate buffer (Sigma) containing 25 mM glucose ...
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bioRxiv - Biochemistry 2019Quote: ... and protein concentration was measured using a Bradford assay (Sigma-Aldrich, Taufkirchen, Germany). Proteins were separated with a 10% SDS-PAGE ...
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bioRxiv - Genomics 2020Quote: ... a specific FAM digital PCR assay was designed (Sigma-Aldrich, Gillingham, Dorset, UK) targeting the promotor region of RASSF1A and five 5’-CGCG-3’ BstUI recognition sequences ...
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bioRxiv - Bioengineering 2021Quote: ... The TUNEL assay was carried out according to the manufacturer’s instructions (Millipore, s7101). For human samples ...
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bioRxiv - Neuroscience 2020Quote: ... Cell death was assessed by TUNEL assay (with TMR, red-dUTP, Sigma, 12156792910) and nuclei were labeled with DAPI stain (Molecular Probes ...
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bioRxiv - Neuroscience 2020Quote: ... was used for dual-luciferase assays and candidate compounds were purchased from Sigma (Sulphaquinoxaline ...
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bioRxiv - Immunology 2021Quote: ... washed in PBS and lysed with radio-immunoprecipitation assay (RIPA) buffer (Sigma-Aldrich) supplemented with protease and phosphatase inhibitors (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: A DPPH-radical scavenging assay using 2,2-diphenyl-1-picrylhydrazyl (DPPH; Sigma-Aldrich) was carried out as previously described [22] with some modifications ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell invasion was measured using the fluorometric QCMTM ECMatrixTM Cell Invasion Assay (Millipore). NB9464-H-2Kblo or NB9464-H-2Kbhi cells in serum-free medium (0.5 x 106 cells/ml ...
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bioRxiv - Molecular Biology 2022Quote: ... Proliferation was evaluated by methyl thiazole tetrazolium assay (MTT, Sigma, Sao Paulo, Brazil). Optical density (OD ...
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bioRxiv - Biochemistry 2022Quote: ... NGM to be used in DTT assays was made using soybean peptone (Sigma 70178 ...
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bioRxiv - Biochemistry 2022Quote: ... Fresh tissue was homogenized and used for a hydroxyproline assay (Sigma-Aldrich, USA). Other parts of the tissue were fixed for 24Lh in 10% formalin and then submerged in 70% EtOH for storage ...
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bioRxiv - Biophysics 2022Quote: ... we formed the assay reagent by mixing 25 mg of bromophenol blue (Sigma), 20 mL of ethanol (HPLC grade ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was collected and protein concentration was measured by Bradford assay (Sigma).
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bioRxiv - Microbiology 2021Quote: Chemicals used for enzymatic assays were from Sigma-Aldrich (St. Louis, MO, USA). All the other reagents were of the highest quality available ...
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bioRxiv - Microbiology 2019Quote: ... Alizarin Red staining assay was performed using Alizarin Red solution (EMD Millipore, Germany) according to manufacturer’s instructions ...
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bioRxiv - Pathology 2019Quote: ... BR) and insulin Elisa assay (cat#EZRMI-13K, Merck Millipore, Billerica, MA, EUA) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Lysates were sonicated and protein concentration was determined using BCA protein assay (Sigma). 30 μg of total proteins were loaded on 12% SDS–PAGE gel and separated by electrophoresis ...
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bioRxiv - Pathology 2019Quote: ... Co-IP assays were performed by incubating total proteins with anti-HA (Sigma) agarose followed by column wash and elution according to manufacturer’s instructions (Pierce ...
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bioRxiv - Microbiology 2019Quote: ... The protein concentration in the extract was determined with a Bradford assay (Sigma) and used for normalisation of the SOD activity.
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bioRxiv - Plant Biology 2021Quote: Immunoblot assays were performed using monoclonal rat anti-HA antibody (Sigma Aldrich, 11867423001), mouse anti-Myc antibody (ThermoScientific R950-25) ...
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bioRxiv - Cancer Biology 2019Quote: ... Whole-cell extract was prepared from 1X radioimmunoprecipitation assay lysis buffer (RIPA; Millipore). Extract was clarified by centrifugation at 15,000g for 15 min at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein concentration was measured using a standard Bradford assay reagent (B6916, Sigma Aldrich).40µg total protein were loaded per samples on a pre-cast gradient 4-12% gel (NW04120 ...