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Citations for Millipore Sigma :
7451 - 7500 of 10000+ citations for QuantiChrom β N Acetylglucosaminidase Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... An emulsion was generated by injecting 50 μL of pre-bead-solution in 500 μL of n-hexane with 3% Span®80 (Sigma-Aldrich) using a 100 μL Hamilton Syringe ...
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bioRxiv - Bioengineering 2020Quote: ... collagen type I was extracted from rat tail tendons as freeze-dried sponges according previously published protocols.42,43 The extracted collagen sponges were dissolved in 0.02 N acetic acid (cat. no. 695092, Sigma-Aldrich, St. Louis, MO) to prepare a sterile stock collagen solution ...
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bioRxiv - Biochemistry 2021Quote: ... from the free radioactivity by transferring the cell suspension to a 0.4 mL centrifuge tube (USA Scientific, Ocala, FL) with a layer of 1:37.5 n-dodecane:bromo-dodecane (Sigma–Aldrich, St. Louis, MO) and spinning at 12,535 x g ...
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bioRxiv - Biochemistry 2021Quote: ... Carboxyl-terminal histidine or N-terminal glutathione-S-transferase fusion tags were added using the respective plasmids pET21b (Novagen, Madison, Wisconsin, USA) and pGEX-4T3 (Amersham Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... 5×106 cells/ ml) in N-free medium were seeded on cover slips coated with poly-L-lysine (Sigma, Cat. No. P8920) for 5 minutes followed by fixation with 4% paraformaldehyde solution (Sigma ...
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bioRxiv - Plant Biology 2020Quote: ... 34.5 mL of the liquid fertilizer was mixed with 0.85 g 15N labelled N (98 atom% 15N, in the form of 15NH4 15NO3, Sigma-Aldrich, Buchs, Switzerland). The amount of 15N corresponded to 8% of the total N given ...
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bioRxiv - Microbiology 2021Quote: ... and trimethylsilylated shortly before analysis through addition of 20 μl N,O-bis(trimethylsilyl)trifluoroacetamide with 1 % trimethylchlorosilane (BSTFA-TMCS, Cerilliant, Sigma B-023). The GC-MS instrumentation and settings (electron ionization mode ...
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bioRxiv - Cell Biology 2020Quote: ... IMR90 cells (n=2 biological replicates) stably expressing ZNF416-FLAG were cross-linked with 1% formaldehyde (Sigma Aldrich, St. Louis, MO, USA) for 10 minutes ...
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bioRxiv - Microbiology 2022Quote: ... a mouse anti-SARS-CoV-2 nucleocapsid (N) protein primary antibody and an anti-mouse IgG HRP secondary antibody in conjunction with SIGMAFAST™ OPD developing solution (Millipore Sigma) permitted SARS-CoV-2 infection quantification ...
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MEK inhibitor resistance in lung cancer cells associated with addiction to sustained ERK suppressionbioRxiv - Cancer Biology 2022Quote: ... MK-2206 (Selleckchem, S1078), NSC 23766 (Selleckchem, S8031), dabrafenib (Selleckchem, S8031), infigratinib (Selleckchem, S2183) and N-acetyl-L-cysteine (Sigma-Aldrich, A7250) were added to cells when indicated ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was isolated from tendon-like constructs (n=4 animals, 3 constructs each) using TRI® Reagent (Sigma-Aldrich; Vienna, Austria) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: FLAG-CAHS1 was constructed using standard genetic engineering techniques with an N-terminal FLAG using a pT7-FLAG-1 vector (Sigma-Aldrich, Merck). The bacterial cells of E ...
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bioRxiv - Bioengineering 2019Quote: Samples were taken from scaffolds using a 2mm biopsy punch (n=5) and incubated with 1% alizarin red s (Sigma Aldrich, A5533) in distilled water for 10 min at a pH of between 4.1 - 4.3 ...
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bioRxiv - Zoology 2019Quote: The midgut tissue dissected out from continuous toxin-exposed susceptible and toxin exposed insects for 6 generations (n=5) were processed for RNA isolation using TRI reagent TM (Sigma Aldrich, USA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Swiss-Webster mice (n=5/group) were injected with 75 µg of ferrous chloride (FeCl2·4H2O, Sigma-Aldrich Inc., St. Louis, MO) by the intraperitoneal (i.p. ...
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bioRxiv - Microbiology 2020Quote: ... acidophilum were PCR-amplified from their respective genomic DNA template and inserted into a pET16b vector containing 10x histidine tag at the N-terminus (Novagen, Madison, WI). The following primers were used ...
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bioRxiv - Microbiology 2020Quote: ... N-acetyl-L-cysteine (NAC) supplemented medium were prepared at the final concentration of 1 mM (A9165, Sigma (Shaposhnikov et al. 2018)).
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bioRxiv - Neuroscience 2021Quote: ... BMDM were treated with either 5μm DPC333 (Qian et al., 2007) O/N or with 50ng/ml PMA (Sigma Aldrich, 19-144) for 30 minutes ...
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Versatile and Robust method for Antibody Conjugation to Nanoparticles with High Targeting EfficiencybioRxiv - Biochemistry 2021Quote: ... N-(3-dimethylaminopropyl)-N’-ethyl-carbodiimide (EDC, 97%), Dimethyl sulfoxide (DMSO, >99,5%) and 3D Petri Dish ® - Microtissues were purchased from Sigma Aldrich (Germany). Dulbecco’s modified eagle medium (DMEM) ...
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bioRxiv - Biochemistry 2021Quote: ... 100 μl of dilution enzyme solution (0.5 μM) was combined with 100 mg/ml N-acetyl-D-glucosamine (Sigma Aldrich, A3286-5G). Cells were allowed to sit on ice for 5 minutes to allow WGA to bind fully ...
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bioRxiv - Neuroscience 2021Quote: ... For immunostaining cells were fixed with 4% PFA in cytoskeleton preservation buffer (10mM 2-(N-morpholino) ethanesulfonic acid (MES, Sigma-Aldrich, M3671) pH 6.1 ...
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bioRxiv - Immunology 2020Quote: ... embryos collected from rpe65a:nfsB-eGFP outcrosses were incubated in system water treated with 1.5X n-phenylthiourea (PTU; Sigma-Aldrich, St. Louis, Missouri) from 0.25 – 5 days post-fertilization (dpf ...
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bioRxiv - Molecular Biology 2021Quote: ... with 1:100 dilution of GlutaMax and penicillin/streptomycin and cells were treated (n = 3 per group) with compounds and tBHQ (Sigma 11294-1) for 24 hr ...
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bioRxiv - Microbiology 2021Quote: N-terminal His-tagged recombinant proteins were expressed in Rosetta™ 2(DE3) pLysS competent cells (71403; Novagen, Inc., Madison, WI, USA) by induction with 0.5 mM isopropyl β-d-1-thiogalactopyranoside at 30°C for 4 h (N ...
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bioRxiv - Bioengineering 2022Quote: ... cell-seeded wells (n=3 per group) were rinsed with PBS and incubated in 500 μL of 5% trichloroacetic acid (TCA, Sigma-Aldrich, T6399) in UPW for 30 minutes ...
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bioRxiv - Physiology 2022Quote: ... a sharp glass pipette (tip inner diameter: 15–30 μm) filled with NMDA (N-methyl-D-aspartate, Sigma-Aldrich, M3262, 0.2 mM), C21 (200 µg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... 0.5 % sodium deoxycholate, 0.1 % SDS, 1X protease inhibitor (GenDEPOT, P3100), 1X phosphatase inhibitor (GenDEPOT, P3200) and 50 mM N-ethylmaleimide (Sigma-Aldrich, E3876-5G) using a Dounce homogenizer ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated o/n at 4°C with the primary antibodies (Rabbit-antiGFAP: MAB3402, Merck Millipore, 1:500; Mouse-antiNeuN: MAB377, Merck Millipore, 1:500) diluted in the blocking solution ...
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bioRxiv - Cell Biology 2024Quote: ... A 3X proteinase buffer was prepared (For 1 ml: 300 µl PBS, 300 µl 20% N-Lauryl sarcosine sodium solution (Sigma Aldrich, L7414), 60 µl 0.5 M EDTA ...
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bioRxiv - Neuroscience 2024Quote: ... embryos from all genotypes (N = 7 per genotype) were collected at 40 hpf and fixed with 4% paraformaldehyde (PFA; Sigma, Gillingham, UK) in phosphate buffered saline (PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... from the free radioactivity by transferring the cell suspension to a 0.4 mL centrifuge tube (USA Scientific, Ocala, FL) containing a layer of oil consisting of 1:37.5 n-dodecane:bromo-dodecane (Sigma–Aldrich, St. Louis, MO) and spinning at 12,535 g for 8 s (Beckman E centrifuge ...
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bioRxiv - Developmental Biology 2024Quote: ... incubated in 70% EtOH for 1 hour on a nutator at RT and rinsed in demi-water and stained overnight (O/N) in carmine solution (1g carmine (Sigma, cat. #C1022), 2.5 g aluminum potassium sulphate (Merck ...
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bioRxiv - Biochemistry 2024Quote: ... MIB1 knockout was confirmed by TOPO cloning of the amplified genomic sequence and by the loss of MIB1 signal on western blot with antibodies for the N- and C-terminal portions of MIB1 (Abcam, ab124929; Millipore Sigma, M5948).
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bioRxiv - Cell Biology 2024Quote: ... cells were deproteinated for 15 minutes in 0.1 N HCl and neutralized for 5 minutes in 2X Saline Sodium Citrate buffer (2X SSC) (Sigma Aldrich, Cat # S6639) at room temperature ...
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bioRxiv - Physiology 2023Quote: ... eNOS-independent relaxation was tested in presence of the pharmacologic eNOS inhibitor N-nitro-L-arginine methyl ester (L-NAME, 300 μM; Sigma-Aldrich N5751) either using increasing concentrations of the NO-donor dimethylamine nonoate (DEA-NONOate ...
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bioRxiv - Systems Biology 2022Quote: ... The extracts were dried under a stream of nitrogen gas and derivatized by incubation at 70 °C for 30 min with 100 μl of N,O–Bis (trimethylsilyl) trifluoroacetamide (BSTFA) and 100 μl anhydrous pyridine (Sigma, St. Louis). After the derivatization reaction ...
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bioRxiv - Cell Biology 2023Quote: ... was cloned into a pLIC vectors with a TEV-cleavable N-terminal Glutathione S-transferase tag and transformed into Rosetta™ 2 (pLysS) competent cells (Sigma-Aldrich). GST-Cse4END was purified as described above ...
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bioRxiv - Neuroscience 2023Quote: ... the mice were intravitreally injected with 1.5 μl of 20 mM N-methyl-d-aspartic acid (NMDA) (Sigma-Aldrich, St. Louis, MO). The 1.0mg/mL commercially available morphine solution (M-005-1ML in methanol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Cells were resuspended in cold Ripa buffer supplemented with 1% n-Dodecyl-ß-D-maltoside and complete ethylenediaminetetraacetic acid (EDTA)-free protein inhibitors (Sigma-Aldrich), and incubated at 4°C for 1 hour ...
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bioRxiv - Biophysics 2023Quote: ... The surface of the coverslips was functionalized by amine using the silanization solution of N-(2-aminoethyl)-3-aminopropyltrimethoxysilane (Sigma-Aldrich, 8191720100), acetic acid ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were visualized by TIRF-M in imaging media and stimulated with a final uniform concentration of 20 nM fMLF (N-Formyl-L-methionyl-L-leucyl-L-phenylalanine, Sigma, cat#F3506) to drive PI(3,4,5)P3 production.
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bioRxiv - Immunology 2023Quote: ... and an N-terminal NusA fusion separated by a TEV cleavage in a pET-50b (+) was transformed into tuner (DE3) (Novagen; cat # 70623), BL21(DE3 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 100 µM of the nonspecific nitric oxide synthase inhibitor Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME [Sigma Aldrich], n=5), or 1.0 mM P188 and 100 µM L-NAME (n=5) ...
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bioRxiv - Neuroscience 2024Quote: ... An antibody reactive for the N-terminal amino acids 66-81 of the amyloid precursor protein (APP) (Clone 22C11: Millipore, Billerica, MA) was applied at 1:40K and incubated overnight at 4°C ...
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bioRxiv - Bioengineering 2024Quote: Recombinant active human MMP-9cd (auto-cleavage resistance) 34 with an N-terminal His6x tag was expressed in Rosetta™(DE3) pLysS (Millipore Sigma) cells using a pET-28a(+)-His6x-MMP-9cd vector as previously described 33,35,36 ...
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bioRxiv - Cancer Biology 2024Quote: ... The injected embryos were then incubated at 30°C for 24 - 48 h and allowed to recover in the presence of N-phenylthiourea (Sigma-Aldrich, P7629) to inhibit melanin synthesis ...
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bioRxiv - Physiology 2024Quote: ... Plates were washed with 1 M NaCl for 10 seconds and then stained for 5 hours using 0.2% amido black (Sigma Product N- 3393) in 1 M NaCl ...
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bioRxiv - Immunology 2019Quote: ... Membranes were blocked for 1 hour in milk block solution and then incubated overnight at 4°C with primary antibodies for either β-actin (Sigma A5441, 1:2000), IL-1β (R&D AF401NA ...
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bioRxiv - Immunology 2021Quote: ... 1 mM penicillin-streptomycin and 55 μM β-mercaptoethanol) containing 10 nM either OVA257-264 (H2N-SIINFEKL-OH) or YopE69-77 peptide and brefeldin A (BFA, Sigma, 5 μg/ml) for 4.5 h at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... antibody was used as a pan-neuronal marker and membranes were reprobed with mouse monoclonal anti-β-actin antibody (1:10,000; Sigma, Saint-Quentin Fallavier, France) to confirm equal protein loading ...