Labshake search
Citations for Millipore Sigma :
7101 - 7150 of 10000+ citations for QuantiChrom β N Acetylglucosaminidase Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2019Quote: ... GTPases were incubated at 37 °C in the presence of 10 mM EDTA and a ten-fold molar excess (nucleotide:protein) of guanosine 5′-[β,γ-imido]triphosphate (GMPPNP, Sigma Aldrich), guanosine 5′-[γ-thio]triphosphate (GTPγS ...
-
bioRxiv - Genomics 2020Quote: ... 3 million CD4+ T cells were incubated in the presence of 5,6-Dichloro-1-β-D-ribofuranosylbenzimidazole (DRB, Sigma-Aldrich, Cat: D1916) at a final concentration of 65μM or Triptolide (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2021Quote: ... and GUS activity was measured after 30 minutes of incubation in the presence of 1 mM 4-methylumbelliferyl-β-D-glucuronide (MUG, Sigma Aldrich). Reactions were stopped by the addition of four volumes of 0.2 M Na2CO3 and fluorescence was measured in a Biotek Synergy microplate reader with excitation and emission wavelengths of 365 nm and 455 nm ...
-
bioRxiv - Neuroscience 2020Quote: ... the sections were incubated overnight at 4°C with primary antibodies (anti-β III tubulin mouse monoclonal, Sigma, T7816 and anti-DCX, Guinea Pig polyclonal, Millipore, AB5910) at dilution of 1:1000 in 1% BSA in PBST (0.5% triton added to PBS) ...
-
bioRxiv - Microbiology 2021Quote: ... and the cells were lysed in TX-100 lysis buffer (300mM NaCl; 50mM Tris-HCl, pH 7.5; 0.5% Triton X-100; β-mercaptoethanol, Sigma protease inhibitor cocktail) for 10 min on ice ...
-
bioRxiv - Neuroscience 2020Quote: ... Each sample was diluted 1/10 with McIlvaine citrate-phosphate buffer pH 4.5 and activity assayed with 4-methylumbelliferyl-2-acetamido-2-deoxy-β-D-gluco-pyranoside (Sigma, Dorset, UK) in a plate reader.
-
bioRxiv - Neuroscience 2021Quote: ... Then the membrane was stripped and probed again with primary antibodies (p44/42 MAPK, Cell Signaling 9102, 1:4000 dilution, β-actin 1:10,000, A5316, Sigma-Aldrich).
-
bioRxiv - Cell Biology 2021Quote: ... Primary antibodies (anti-CD31 #77699, anti-Pdgfrβ #3695 from Cell Signaling; anti-GFAP #63893 and anti-β-actin #A5441 from Sigma Aldrich; anti-Sm22 #14106 from Abcam and anti-Iba1 #01919741 from Wako ...
-
bioRxiv - Cell Biology 2021Quote: ... Between 22,109 and 26,665 FACS-sorted β-cells were then lysed in Laemmli buffer and loaded on tricine gels for detection of insulin (Sigma, I2018), SNAP tag (NEB ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were treated with NGF (100ng/mL) for 1 hour prior to treatment for 15 minutes with 10µM β-chlornaltrexamine (CNA, Sigma-Aldrich, #O001) or CNA followed by 10µM SNC80 (Tocris ...
-
bioRxiv - Neuroscience 2022Quote: ... Pregnant Dbh+/− dams were administered drinking water containing the β-adrenergic receptor (AR) agonist isoproterenol and the α1AR agonist phenylephrine (20 μg/ml each; Sigma-Aldrich) with vitamin C (2 mg/ml ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were lysed with RLT plus lysis buffer (RLT plus buffer, Qiagen, Valencia, CA containing β-mercaptoethanol, M3148, Sigma, St. Louis, MO), and stored at −80°C until processing for gene expression analysis.
-
bioRxiv - Microbiology 2020Quote: ... was used for Western blotting at a dilution of 1:4,000 and mouse monoclonal antibody against β–actin (Sigma, St. Louis, MO) was used for Western blotting at a dilution of 1:2,000 ...
-
Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... 0.25% Triton-X 100) and 4-Methylumbelliferyl β-D-glucopyranoside stock solution (30 mM; Sigma-Aldrich, M3633-1G, stock solution in DMF) was diluted 3-fold in the GCase activity buffer ...
-
bioRxiv - Cell Biology 2021Quote: ... We then centrifuged the homogenate at 210 g for 10 min at room temperature and resuspended the pellet in complete medium supplemented with 10 μM of cytosine- β-D-arabinofuranoside (Sigma-Aldrich) to prevent fibroblast growth ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... L-ascorbic acid (A0278), ammonium iron (II) sulfate hexahydrate (215406), BSA (A3803), and β-nicotinamide adenine dinucleotide (NAD, N6522) were from Sigma-Aldrich. Tween-20 (10% ...
-
bioRxiv - Plant Biology 2022Quote: ... 5 μL of cell lysate was added to 45 μL of 10 mM 4-methylumbelliferyl-β-D-glucuronide solution (MUG, M-9130; Sigma-Aldrich). The reaction was stopped after 1 hour incubation at 37°C by adding 220 μL of 0.2 M Na2CO3 ...
-
bioRxiv - Plant Biology 2022Quote: ... per 0.25 g of grounded tissue was mixed with 1ml extraction buffer (50mM Tris, pH 7.5, 150 mM NaCl, 5mM EGTA, 5mM EDTA 0.3% β-mercaptoethanol, 1% Sigma Plant Protease Inhibitor), the mixture was homogenized for 10s using an Omnitip homogenizer on ice ...
-
bioRxiv - Plant Biology 2022Quote: ... Groundtissue was mixed with 500 µL extraction buffer (50mM Tris, pH 7.5, 150 mM NaCl, 5mM EGTA, 5mM EDTA 0.3% β-mercaptoethanol, 1% Sigma Plant Protease Inhibitor) and homogenize immediately by Omnitip homogenizer for 10s on ice ...
-
bioRxiv - Physiology 2019Quote: ... transgenic soybean hairy roots and tobacco leaves were used to determine GUS enzyme activity by measuring the fluorescence of 4-methylumbelliferone (4-MU) produced by GUS cleavage of 4-methylumbelliferyl-β-d-glucuronide (4-MUG, Sigma, USA) according to the published procedure as described previously (Jefferson ...
-
bioRxiv - Biochemistry 2020Quote: ... Each rat was tail vein cannulated in order to receive 2-Hydroxypropyl-β-cyclodextrine-cyclodextrine (HBC) followed by Corticosterone (CORT) at 2.5mg/kg (Sigma-Aldrich. Switzerland) in order to imitate the dose of steroid hormones in the plasma induced by substantial stress ...
-
bioRxiv - Neuroscience 2019Quote: ... the medium was replaced with fresh feeding medium containing a final concentration of 5 μM cytosine β-D-arabinofuranoside (AraC; Sigma-Aldrich) for 24 hours to inhibit the growth of non-neuronal cells ...
-
bioRxiv - Cell Biology 2019Quote: ... The beads were then washed in Rac buffer and incubated with 1 mM GTPγs (Guanosine 5ʹ-[β,γ-imido]triphosphate trisodium salt hydrate, Sigma-Aldrich) overnight at 4 ° C in the presence of 15 mM EDTA and 100 units of CIP ...
-
bioRxiv - Microbiology 2020Quote: ... The lactose was hydrolyzed with a β-galactosidase (used above) and the resulting glucose was oxidized with a glucose oxidase (Sigma Aldrich) concomitant with the production of H2O2 measured by coupling to horseradish peroxidase (Sigma Aldrich ...
-
bioRxiv - Synthetic Biology 2020Quote: ... The absorbance at 580 nm (violacein) and 440 nm (β-carotene) was measured and compared to respective standard curves with violacein (Millipore Sigma) and β-carotene (Millipore Sigma ...
-
bioRxiv - Pathology 2020Quote: ... Proteins were immunoblotted with antibodies against FECH (rabbit, 1:400, LS Bio, #LSC409953, and β-actin (mouse, 1:5000, Sigma-Aldrich, AC40). Secondary antibodies ...
-
bioRxiv - Immunology 2020Quote: Murine Hoxb8 is a myeloid progenitor cell line that can be differentiated to monocytes or macrophages upon removal of β-estradiol (Sigma-Aldrich) (Wang et al. ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... We diluted the saturated culture 1:10 in 96-well plates containing 200 μL of SC[MSG] -ura -leu containing Estradiol (β-estradiol, Sigma-Aldrich) at different concentrations (0 ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... 10 μM L-ascorbic acid 2-phosphate (Wako Chemicals; Richmond, VA) and 10 mM β-glycerol phosphate (Millipore Sigma (St. Louis, MO). Triplicate wells were exposed to differentiation media without any agent (appropriate vehicle only-control) ...
-
bioRxiv - Bioengineering 2019Quote: ... Cells were cultured within the devices for 21 days in αMEM osteogenic media (10% FBS, 1% penicillin-streptomycin, 10 mM β-glycerophosphate (Sigma-Aldrich), 50 μM ascorbic acid (Sigma-Aldrich) ...
-
bioRxiv - Neuroscience 2021Quote: Odorants were excluded from all analysis if no ORs were identified as responsive at the tested concentrations: 1% β-Caryophyllene (Sigma W22520715), 1% dimethyl sulfide (Sigma 274380) ...
-
bioRxiv - Pathology 2021Quote: ... (groups 2 & 3) treated groups including mice receiving daily intraperitoneal injection of 150 mg/kg/day of β-aminopropionitrile fumarate (BAPN, A3134; Sigma Aldrich) for 28 days ...
-
bioRxiv - Neuroscience 2021Quote: ... This antiserum detects human α-CGRP and β-CGRP but does not cross-react with any other peptide (data supplied by Sigma). We used a rabbit polyclonal antibody (1:1000 ...
-
bioRxiv - Cell Biology 2021Quote: ... Primary antibodies (diluted in 5% BSA in 1X PBS-T) were as follows: Mouse anti-β-Actin (1:10,000, Sigma-Aldrich, A5441), Rat anti-α Tubulin (1:2000 ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cells were co-incubated with β-Nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate (NADPH; 1 mM; N7505, Sigma-Aldrich) and HET0016 (10 μM ...
-
bioRxiv - Neuroscience 2022Quote: ... glia-containing samples were cultured with NBD supplemented with 2 μM of cytosine-β-D-arabinofuranoside (AraC, 500 μM in ultrapure water, C1768, Sigma Aldrich) to inhibit astrocyte and microglia proliferation ...
-
bioRxiv - Bioengineering 2022Quote: ... The reaction was started by adding 30 µL of 100 mM (final concentration 5 mM) substrate 4-nitrophenyl β-D-glucopyranoside (Sigma-Aldrich). At regular five-minute intervals ...
-
bioRxiv - Bioengineering 2022Quote: ... Cells were stimulated to undergo osteogenic differentiation by providing osteogenic differentiation medium (osteogenic control medium with osteogenic supplements 10 mM β-glycerophosphate (G9422, Sigma-Aldrich), 50 μg/ml ascorbic acid-2-phosphate (A8960 ...
-
bioRxiv - Biochemistry 2022Quote: ... Then the gene expression was induced by the addition of 1 mM (final concentration) of isopropyl-β-D-1-thiogalactopyranoside (IPTG, Sigma-Aldrich). After 3 h of induction at 37 °C ...
-
bioRxiv - Bioengineering 2022Quote: ... or fresh media was applied (either expansion medium or osteogenic medium [expansion medium + 100nM dexamethasone, 50 mg/mL ascorbic acid, and 10mM β-glycerol phosphate; all Sigma-Aldrich]) and cultured for a further 7 days ...
-
bioRxiv - Developmental Biology 2022Quote: ... and cultures were incubated overnight at 4°C with primary antibodies diluted in TBS/10% goat serum: mouse anti-β-tubulin-III (Sigma #T8660) or mouse anti-MAP2 (Sigma ...
-
bioRxiv - Immunology 2022Quote: ... we used a synthetic cathepsin C inhibitor (IcatCXPZ-01; (37) diluted in 10 % (2-Hydroxypropyl)-β-cyclodextrin (CAS: 128446-35-5; C0926; Sigma-Aldrich) suspended in citrate buffer 50 mM at pH=5 (vehicle ...
-
bioRxiv - Immunology 2022Quote: ... BLaER1 cells were trans-differentiated for 5-6 day in medium containing 10 ng/mL IL-3, 10 ng/mL M-CSF (MPI of Biochemistry, Munich) and 100 nM β-estradiol (Sigma-Aldrich) at a concentration of 7 × 104 cells/well in 96 well plates ...
-
bioRxiv - Neuroscience 2023Quote: ... equivalent to about 5 mg/kg/day) was prepared in vehicle made of 0.45% (2-hydroxypropyl)-beta-cyclodextrin (β-CD; both from Sigma-Aldrich, France), and administered in drinking water for 28 days ...
-
bioRxiv - Plant Biology 2022Quote: ... Peak area of carotenoids was selected based on the spectral characteristics and then calculated according to standard compounds (lutein, lycopene, and β-carotene; Sigma, USA).
-
bioRxiv - Molecular Biology 2022Quote: Gus activity was determined to quantify chitin binding of Gus-Cts1 using the specific substrate 4-methylumbelliferyl β-D galactopyranoside (MUG, Sigma–Aldrich). To his end 50 μg of U ...
-
bioRxiv - Neuroscience 2023Quote: ... horizontal shaker) in digestion medium (RPMI-1640 supplemented with L-glutamine, Pen/Strep, β-ME, HEPES, Sodium Pyruvate, Liberase TLTM (Sigma Aldritch) and DNase I) ...
-
bioRxiv - Microbiology 2024Quote: ... The incubated samples were aliquoted into 2 tubes (500 μL each) and water-soluble cholesterol (25 μg) (cholesterol/methyl-β-cyclodextrin; Sigma-Aldrich) was added to only one of the samples and both samples were incubated at 37°C for 30 min ...
-
bioRxiv - Cell Biology 2024Quote: ... 21-day old (p21) triple transgenic mice (β-catistab) were given 2 mg/ml doxycycline in water (Sigma-Aldrich, St. Louis, MO) and 6 g/kg of dietary doxycycline in rodent chow (Envigo-Harlan ...
-
bioRxiv - Neuroscience 2024Quote: ... and pregnant Dbh +/- dams were administered drinking water containing the β-adrenergic receptor (AR) agonist isoproterenol and the α1AR agonist phenylephrine (20 μg/ml each; Sigma-Aldrich) with vitamin C (2 mg/ml ...