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Citations for Millipore Sigma :
6551 - 6600 of 8078 citations for L Asparagine H2O 13C4 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... with and without the addition of tryptophan (100 μL of the 100 mg L−1 stock solution in distilled water and filtered through 0.22 μm Millipore membrane ...
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bioRxiv - Microbiology 2020Quote: ... Pellets were resuspended in 500μL S-Trap lysis buffer (5% SDS, 50mM TEAB, pH 7.5) supplemented with cOmplete EDTA-free protease inhibitor (Sigma) and HALT phosphatase inhibitor cocktail (Thermo Fisher) ...
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bioRxiv - Plant Biology 2021Quote: ... seeds were plated on solid growth media (GM) consisting of g/L Murashige and Skoog basal salt mixture (Sigma), 1% sucrose ...
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bioRxiv - Plant Biology 2020Quote: ... clv2 and fin mutants was conducted using half strength LANS containing 2.5 mM NaH2PO4 and 5.6 mM KNO3 (Hewitt 1966) solidified with 5g phytagel/L (Sigma, USA). In subsequent experiments that aimed to examine the tomato root morphology response under different N conditions ...
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bioRxiv - Microbiology 2020Quote: Approximately 20 µL of cells were air-dried either on agarose pads (1% (w/v) in PBS) or on slides coated with 0.1% (w/v) poly-l-lysine solution (Sigma) and bacterial nucleoids stained by topping cells with a drop of Fluoroshield™ containing 1.5 µg/mL 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Neuroscience 2020Quote: Fresh solutions of L-NAME were prepared daily in accordance with previously published protocols (López-Juárez et al., 2017; L-NG-nitroarginine methyl ester, 100 mM in 13 PBS; Sigma-Aldrich) and injected to animals intraperitoneally (0.4 mg/kg ...
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bioRxiv - Microbiology 2021Quote: ... The resulting construct was transformed into protoplasts of each strain and mutants were selected for on osmotically stabilized minimal media (GMM plus 1.2M sorbitol) containing 100 μg/L pyrithiamine hydrobromide (Sigma). Reconstitution of the alaA gene was performed by PCR amplification of the alaA locus for each strain from ∼1.1kb upstream of the start codon to ∼700bp downstream of the stop codon using primers containing PacI and AscI digestion sites ...
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bioRxiv - Systems Biology 2020Quote: ... the MPAO1 cryoculture was streaked out on 20% BHI solid medium (7.4 g in 1 L water) containing 1.5 % agar (both Sigma, Switzerland). Shaken 20% BHI fluid cultures were inoculated from a single colony and grown at 30 °C until mid-exponential phase (OD600 = 0.5) ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with selection medium (SM, 8 mM L-glutamine and phenol red, with 0.5 mg/mL G-418, Sigma). Cell pools were maintained at 37°C ...
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bioRxiv - Cell Biology 2021Quote: Neurospheres were dissociated to single-cell and were seeded on poly-L-ornithine/laminin (Sigma, Saint Louis, Missouri, USA) coated plates for 24h in neurosphere medium ...
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bioRxiv - Biophysics 2021Quote: ... cells were grown in a 1-L Braun Biostat fermenter supplemented with 15NH4Cl and D-[13C] glucose (Sigma-Aldrich) as the sole source of nitrogen and carbon for the bacterial cells ...
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bioRxiv - Biophysics 2020Quote: ... A single colony isolate was grown overnight and then sub-cultured in 50 ml tryptone media (0.015g/l; Sigma) till mid-log phase (OD 600 at 200 rpm ...
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bioRxiv - Cancer Biology 2020Quote: ... β-actin-tTSKid//– (MR) mice were maintained on drinking water containing tetracycline hydrochloride (100 mg/L) (Sigma-Aldrich, T7660) and 3% sucrose (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... Single cell suspension was resuspended in BrainPhys complete media and 1.13×105 cells/cm2 were seeded in plates coated with 15μg/mL Poly-L-Ornithine (Sigma #P4957) and 0.5μg/cm2 laminin (Gibco #23017-015) ...
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bioRxiv - Cancer Biology 2020Quote: Cofluent astrocytes were irradiated with 0 Gy or 10 Gy followed by 10 days of culture on 0.2% gelatin in astrocyte medium supplemented with 50 µg/ml L-ascorbic acid (Sigma). After 10 days ...
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bioRxiv - Microbiology 2022Quote: ... UK) supplemented with 0.05 mg/mL mupirocin and 0.05 mg/mL of L-cysteine (both from Sigma-Aldrich, UK) within four hours of collection ...
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bioRxiv - Cell Biology 2022Quote: ... with 200 U/ml penicillin and 200 µg/ml streptomycin and 200 nM L-Glutamine and 0.5 % bovine serum albumin for the NIH3T3 cells and DMEM:F12 (Sigma D8437) supplemented with 0.3% BSA ...
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bioRxiv - Cell Biology 2022Quote: ... 500 μg/mL rice-derived recombinant human albumin and 213 μg/mL L-ascorbic acid 2-phosphate (Sigma-Aldrich). After 24 hours of CHIR99021 stimulation ...
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bioRxiv - Neuroscience 2022Quote: ... Because mouse astrocytes efficiently stimulate synaptogenesis in human induced neurons,30 cultures from P1 to P3 mice were prepared two to three weeks earlier with Trypsin digestion followed by mechanical dissociation and maintained in astrocyte medium: DMEM with 4.5 g/l glucose supplemented with pyruvate (1 mM, Sigma), heat inactivated fetal calf serum (10% ...
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bioRxiv - Cell Biology 2022Quote: ... washed and eluted by the addition of 2 x 1.5 ml of 10 mM reduced L-glutathione (Sigma #T6066) in 50 mM Tris pH 8 ...
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bioRxiv - Bioengineering 2022Quote: ... and 1% PS dropped on Si and glass materials coated with 0.01% poly-L-lysine (PLL, No.P8920, Sigma-Aldrich) and 1–2 μg/cm2 laminin (No.L2020 ...
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bioRxiv - Neuroscience 2022Quote: Mice were lightly anesthetized with isoflurane (0.5%–1% at a flow rate of 0.5 L/min) and 10 μL of Complete Freund’s Adjuvant (CFA, F5881, Sigma Aldrich) was injected subcutaneously into the plantar area of the right hind paw using an insulin syringe (Sure Comfort 29g x 1/2” 3/10cc) ...
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bioRxiv - Neuroscience 2022Quote: The culture surface of each well was coated with poly-L-lysine (0.5 mg/ml, diluted in boric acid buffer, Sigma). Wild type E16.5 C57BL/6J embryos were extracted ...
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bioRxiv - Genetics 2022Quote: ... NSCs were washed with Wash Media (WM) (DMEM/Ham’s F-12 media with l-Glutamine (Sigma-Aldrich, D8437-500), 300 mM D-(+)-Glucose solution (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... cell culture plates were coated overnight with 1 mg/mL poly-L-lysine (Sigma-Aldrich, catalog no. P2636-100MG) and rinsed with diH20 ...
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bioRxiv - Biophysics 2022Quote: ... the cells were fixed with 2% paraformaldehyde/0.1% glutaraldehyde in PBS for 30 min after 1 day of culturing on poly-L-lysine (PLL)-coated (0.1% w/v PLL solution (Sigma) for 30 min at RT ...
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bioRxiv - Biophysics 2022Quote: ... and 0.02% (w/v) yeast extract for 15N labelling or 15NH4C1 and 13C6 D-glucose (3 g/L, Sigma) with 0.02% (w/v ...
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bioRxiv - Bioengineering 2022Quote: MCF-7 cells were cultured in DMEM growth media supplemented with 10mg/L insulin (Sigma Aldrich, St. Louis, MO), 1% v/v Penicillin-Streptomycin mix (Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... were transferred individually into vials containing standard cornmeal diet or nutrient of interest (sucrose: Sigma #S7903, L-glucose: Sigma #G5500 ...
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bioRxiv - Cell Biology 2022Quote: ... 100 μL of medium containing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) (1 mg/mL) (Sigma-Aldrich) was added to each well ...
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bioRxiv - Biochemistry 2021Quote: ... and trypsin concentration was quantified using an enzymatic assay with Nα-Benzoyl-L-arginine ethyl ester (BAEE, Sigma-Aldrich) exactly according to Rocklin et al (Rocklin et al ...
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bioRxiv - Microbiology 2021Quote: ... the cells were resuspended in 50 μL of supernatant and were spotted on coverslips coated with 0.2% L-Poly-lysine (Sigma) in 24-well tissue culture plates (Corning ...
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bioRxiv - Microbiology 2020Quote: ... Kanamycin concentrations when used were at one tenth the standard concentrations on plates (5 mg/L, Sigma Aldrich, USA) and at one fourth the standard concentration in liquid (12.5 mg/L) ...
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bioRxiv - Molecular Biology 2020Quote: ... coated first with Poly-L-ornithine hydrobromide (6 μg/mL in 1xPBS, 1 h at 37 °C, Sigma-Aldrich) and then with Laminin from Engelbreth-Holm-Swarm murine sarcome basement membrane (1.2 μg/mL in 1xPBS ...
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bioRxiv - Molecular Biology 2020Quote: ... and hippocampal cells on 22 mm glass coverslips (150,000 cells/coverslip) coated with poly-L-lysine (P2636; Sigma-Aldrich) in 2 ml plating medium (Neurobasal medium (21103-049 ...
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bioRxiv - Immunology 2020Quote: ... with L-glutamine media supplemented with 10% fetal bovine serum (FBS) and 1% Penicillin/Streptomycin (Sigma, St. Louis, MO). To detach confluent cells ...
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bioRxiv - Bioengineering 2021Quote: ... were homogenized at 35000 rpm for 60 sec using a TH homogenizer (Omni International) in buffer (1 mL, pH 6.5) containing papain (25 μg m/l, Sigma), l-cysteine (2 × 10-3M ...
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bioRxiv - Biochemistry 2021Quote: ... synthetic complete (SC) medium (2% glucose, 6.7 g/L yeast nitrogen base without amino acids and with ammonium sulfate (Sigma)) supplemented with 1.92 g/L Drop-out Mix Synthetic (US Biological ...
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bioRxiv - Plant Biology 2021Quote: ... Seeds were plated on Murashige and Skoog (MS) media (Caisson, MSP01) supplemented with Gamborg’s half-strength vitamin solutions (0.5 mL/L) (Sigma Aldrich), 0.5% phytagel (Sigma-Aldrich ...
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bioRxiv - Biophysics 2020Quote: ... which was pretreated with 0.01% poly-L-lysine (mol. wt. 150 K–300 K; Millipore Sigma, St. Louis, MO) at room temperature for 10 min ...
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bioRxiv - Cancer Biology 2020Quote: ... was added as an internal standard and dried extracts were derivatized with diacetyl-L-tartaric anhydride (DATAN, Sigma-Aldrich). HPLC-ESI-MS detection was conducted on a ThermoFisher Q Exactive mass spectrometer with on-line separation by a ThermoFisher Dionex Ultimate 3000 HPLC ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The neuron suspension was then plated on poly-L-lysine and laminin coated 12 mm cover glass (Sigma, Australia) and left to attach for ~3 h at 37 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... and 100 µmol/L Palmitic acid (conjugated to bovine serum albumin within B27 supplement by incubating for 2 hours at 37ºC, Sigma) with changes every 2-3 days ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were plated for experiment at a density of 50,000 cells/cm2 onto 100 μg/ml poly-L-ornithine (PO, Sigma) and 20μg/ml laminin-coated plates and media was changed every 2-3 days ...
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bioRxiv - Immunology 2022Quote: ... with L-glutamine media supplemented with 10% fetal bovine serum (FBS) and 1% Penicillin/Streptomycin (Sigma, St. Louis, MO). To detach confluent cells ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The initial populations were mixed (1:1) followed by diluting 400-fold in 200 µl of NaCl 0.9% with L- arabinose 0.5 % (Sigma, Spain) and incubated at 37 ºC at 250 rpm during 1.5 hours to induce GFP expression ...
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bioRxiv - Neuroscience 2022Quote: ... dissociated cortical neurons were plated onto tissue culture dishes coated with 1mg/mL poly-L-lysine (PLL, Sigma-Aldrich). Neurons were maintained in at 37℃/5% CO2 ...
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bioRxiv - Physiology 2022Quote: ... hatching was induced by placing embryos in chilled (4°C) 1 g/L humic acid solution (Sigma-Aldrich, 53680) and incubating them in that solution overnight at room temperature ...
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bioRxiv - Immunology 2022Quote: ... cells using minimal essential medium supplemented with 0.2% bovine serum albumin and 2 μg/mL N-Tosyl-L-phenylalanin-chlormethylketon (Sigma). For the determination of the tissue culture infectious dose 50 (TCID50) ...
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bioRxiv - Biophysics 2022Quote: ... the device was instead coated with a 0.5 mg/mL of PLL solution in PBS diluted from Poly-L-lysine solution 0.1 % (w/v) (Sigma # P8920). Confiner coverslips and glass-bottom dishes were rinsed and incubated in medium for at least 2 hours before confining the cells.