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Citations for Millipore Sigma :
6501 - 6550 of 8078 citations for L Asparagine H2O 13C4 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... for lentiviral packaging were expanded to 70-85 % confluency in DMEM Glutamax (+ 4.5 g/L D-Glucose, - Pyruvate) supplemented with 10 % FBS (Sigma), 1 % G418 (Gibco) ...
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bioRxiv - Genetics 2021Quote: The S2 cells used for colcemid-induced MT depolymerization were grown 39.4 g/L Shields and Sang M3 Insect medium (Sigma) supplemented with 0.5 g/L KHCO3 and 20% heat-inactivated FBS (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... Focal demyelinating lesions were induced by stereotaxic injection of 0.8 μl 1% lysolecithin solution (L-a-lysophosphatidylcholine, Sigma L4129) into the corpus callosum at coordinates ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 µl of total RNA (2.5µg/µl) were added to 20 µl of “glyoxal mix” (60% DMSO, BTPE 1.2X, 5% glycerol, 8% glyoxal solution (Sigma) and 40 µg/ml Ethidium bromide) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... which was pretreated with 0.01% poly-L-lysine (mol. wt. 150 K–300 K; Millipore Sigma, St. Louis, MO) at room temperature for 10 min ...
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bioRxiv - Bioengineering 2022Quote: ... and the suspension was disseminated on a culture dish coated with 15 μg/ml poly-L-ornithine (Sigma-Aldrich) and 1 μg/ml human fibronectin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were plated at a density of 6×104 cells / cm2 on glass coverslips coated with 0.2mg/ml Poly-L-lysine (#P2636-100MG Sigma) made in Borate buffer pH 8.5 ...
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bioRxiv - Neuroscience 2021Quote: ... After isolation 200,000 neurons were plated onto circular glass coverslips (Electron Microscopy Services) pre-coated with poly-L-lysine (Sigma) and mouse laminin (Thermo Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... The neurons were quantified and centrifuged at 1,800 rpm for 6 min and then plated on the glass coverslips pre-coated with poly-L-lysine (Sigma). The culture medium was Neurobasal (Gibco ...
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bioRxiv - Neuroscience 2021Quote: ... in 16-well stripes (500,000 cells/well) and re-plated on glass coverslips coated with 100 μg/ml Poly-L-Lysine (Sigma) and 1 μg/ml Laminin (Sigma) ...
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bioRxiv - Neuroscience 2021Quote: ... in 16-well stripes (500,000 cells/well) and re-plated in plastic dish (Costar) coated with 100 μg/ml Poly-L-Lysine (Sigma) and 1 μg/ml Laminin (Sigma) ...
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bioRxiv - Molecular Biology 2021Quote: ... and 3 μl cold lysisg/μl cold lysisl U6.3>Lhx5/Sox1/Six3 gRNA f+e was microinjected together with the Fast Green solution (Sigma) at a 2:1 ratio to ease the detection of the injection site of the developing neural tube and eye respectively with the help of borosilicate glass capillaries and electroporated as previously reported86–88 using the Intracel TSS20 OVODYNE Electroporator ...
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bioRxiv - Cell Biology 2022Quote: ... for lentiviral packaging were expanded to 70-85% confluency in DMEM Glutamax (+4.5 g/L D-glucose, - pyruvate) supplemented with 10% FBS (Sigma), 1% G418 (Gibco) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were plated on 18-mm glass coverslips coated with laminin (1.25 mg/ml) and poly-L-lysine (37.5 mg/ml)(P8920 Sigma Aldrich) at a 50K/well density ...
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bioRxiv - Developmental Biology 2022Quote: Primary antibodies were used at the following concentrations: 1:100 anti-laminin (laminin α-1, RRID:AB_477163, Sigma L-9393), 1:100 anti-GFP (RRID:AB_94936 ...
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bioRxiv - Developmental Biology 2022Quote: ... For dSTORM testicular cells were spread on 1.5 thickness round coverslips with high precision (170±5 μm) coated with 0.01% poly-L-lysine (Sigma, P8920).
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bioRxiv - Zoology 2019Quote: ... Ten sexually mature individuals were chosen from each stock to spawn by flowing water stimulation for two hours followed by injection of 0.1 mL of 0.05 mmol/L serotonin (5-hydroxytryptamine, Sigma, USA) into the adductor muscle (Cruz ...
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bioRxiv - Genetics 2019Quote: ... The differentiating cells were split 1:2 with Accutase and seeded with ROCK inhibitor onto Poly-L-ornithine (Sigma), fibronectin (2ug/ml ...
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: ... confluent cells were removed from flasks using Trypsin-EDTA (0.05/0.02%) (Biochrom, Germany) and then transferred onto poly-L-lysine–coated (0.01%) (Sigma-Aldrich, USA) glass coverslips (5000 cells/cm2 ...
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bioRxiv - Bioengineering 2019Quote: ... Synthetic Complete (SC) medium was prepared using 6.7 g/L Yeast Nitrogen Base without amino acids from Sigma-Aldrich, 1.92 g/L Synthetic Drop-out supplement without histidine from Sigma-Aldrich and 76 mg/L histidine ...
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bioRxiv - Microbiology 2019Quote: ... Bacteria-free supernatants were incubated at room temperature for 10 minutes with 20 mmol/l of dithiothreitol (Sigma-Aldrich). Supernatant was aliquoted into two tubes ...
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bioRxiv - Microbiology 2019Quote: One liter from each 60-L seawater sample was filtered through a 0.22-μm pore-size Durapore PVDF 47-mm filter (Millipore) in a sterile Sterivex filter unit (Millipore) ...
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bioRxiv - Developmental Biology 2020Quote: ... resuspended in complete Neurobasal media and plated at 0.5 × 106 cells/well on 6-well plates coated with 0.1 mg/ml poly-L-lysine (PLL; Sigma Aldrich). Neurons were cultured at 37°C in a humidified incubator with 5% CO2 and medium replaced every 5 days with fresh medium containing B27.
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bioRxiv - Synthetic Biology 2020Quote: ... Antifungal marker characterization experiments were conducted in yeast extract-peptone-dextrose medium (50 g/L YPD broth (Sigma-Aldrich)) supplemented with various concentrations (50 µg/mL ...
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bioRxiv - Cancer Biology 2019Quote: ... both cell lines were cultured in RPMI-1640 medium with L-glutamine and sodium bicarbonate (R8758, Sigma-Aldrich, Germany), supplemented with 10% fetal bovine serum (F7524 ...
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bioRxiv - Microbiology 2019Quote: ... RT-qPCR experiments were conducted with Top Banana cultures cleared of Wolbachia by rearing for two generations on indicated diet with 0.25 g/L tetracycline (Sigma), followed by >10 generations on regular diet ...
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bioRxiv - Cell Biology 2019Quote: ... Then 2,5 Mл culture medium (α-MEM, 10% FBS, 100 μM L-ascorbic acid 2-phosphate (Sigma-Aldrich, USA), 2 mM L-glutamine ...
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bioRxiv - Biophysics 2019Quote: Clean glass coverslips were placed flat on a clean slide and a 100 μL droplet of 0.01% poly-L-lysine (P4832, Sigma) was added ...
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bioRxiv - Molecular Biology 2020Quote: ... The 40 µL RNA sample destined for CMC-labeling was added to 400 µL BEU+CMC buffer (50 mM Bicine, pH 8.5; 4 mM EDTA; 7 M urea; 200 mM CMC (Sigma)) ...
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bioRxiv - Molecular Biology 2019Quote: ... The sections were mounted onto microscope glass slides coated with a 0.01% solution of (poly)L-lysine (Sigma-Aldrich) or adhered to parafilm ...
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bioRxiv - Genetics 2020Quote: ... CAN1 forward mutation rates were determined by plating cells on SCD-Arg medium supplemented with 60μg/mL L-canavanine sulfate (SCD-Arg+Can; Sigma). For complementation studies in Figure 2B ...
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bioRxiv - Cancer Biology 2020Quote: MCF-7 [28] and MDA-MB-231 [29] cells were cultured in high glucose (4.5 g L−1) Dulbecco’s Modified Eagle Medium (DMEM) (Sigma-Aldrich) supplemented with 10 % Foetal Calf Serum (FCS ...
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bioRxiv - Cell Biology 2020Quote: ... The media were supplemented with 10% fetal bovine serum (FBS) and 2 mM L-glutamine (all from Sigma-Aldrich). Cells were cultured at 37 °C and 5% CO2 and regularly tested for mycoplasma contamination.
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bioRxiv - Neuroscience 2020Quote: ... The tissue was transferred to sterile conditions and enzymatically digested in dissociation medium supplied with 0.16 mg/ml L-cysteine and 70U papain (Sigma) set to pH 7.35 ...
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bioRxiv - Neuroscience 2020Quote: ... and iPSC derived H9N NSCs homozygously carrying the LRRK2 G2019S (GGC>AGC) mutation (ax0310) (Axol Bioscience, Cambridge, United Kingdom) were cultured and expanded on 0.01 % poly-L-ornithine (PLO) (Sigma) and L-15 laminin (L15 medium containing 1:60 natural mouse laminin and 1:41 sodium bicarbonate ...
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bioRxiv - Neuroscience 2021Quote: ... Bottles were filled with either protein-containing solution (4% casein, sodium salt from bovine milk, Sigma #C8654; 0.21% L-methionine, Sigma #M9625 ...
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bioRxiv - Neuroscience 2020Quote: ... Enucleated eyes were placed in petri dish filled with carboxygenated (95% O2/ 5% CO2) Ames’ media (supplemented with 1.9g/L sodium bicarbonate, pH 7.4, Sigma Aldrich) and retinas dissected ...
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bioRxiv - Cell Biology 2021Quote: ... A 1-μl aliquot of each sample was put on a poly-L-lysine coated glass slide (Sigma, P0425) and observed by confocal microscopy.
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bioRxiv - Bioengineering 2021Quote: RBCs samples were bonded to Nº 1.5 glass coverslips treated with 100 ng/ml Poly-L-Lysine (Sigma-Aldrich) for 1 h ...
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bioRxiv - Biochemistry 2021Quote: ... Detergents in the samples were exchanged with 100 mmol/L Tris-HCl (pH 8.5) using Microcon filters (10 kDa cut off, Millipore). Tryptic digestion with trypsin (Sequencing Grade Modified ...
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bioRxiv - Biochemistry 2021Quote: Frozen helminths were ground with a pestle in a mortar with liquid nitrogen to a powder with the addition of 0.1 mol/L Tris-HCl (pH 7.6) with 1% protease inhibitor cocktail (Amresko) and 1% phenylmethanesulfonyl fluoride (Sigma-Aldrich). After thawing the extraction buffer protein was precipitated with 100% trichloroacetic acid (final concentration 10%)(Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... and the animals mildly anesthetized with a dose of tricaine methane sulfonate (MS-222; 80 mg/L; Sigma-Aldrich) to facilitate their placement in the tube and minimize their stress ...
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bioRxiv - Neuroscience 2020Quote: ... The dissociated cells were plated at a density of 20000-30000 cells/cm2 on poly-L-lysine (P2636, Sigma) (0.2mg/ml in borate buffer ...
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bioRxiv - Neuroscience 2021Quote: ... The surfaces of glass coverslips and plates were pre-coated with 1 μg/μl poly-L-lysine (Sigma-Aldrich).
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bioRxiv - Neuroscience 2019Quote: ... Neurons were then plated on glass coverslips (WVR international) previously coated with PLL (Poly-L-lysine hydrobromide, mol wt 70.000-150.000, Sigma Aldrich) diluted to 1mg/ml in borate buffer (0.1M boric acid ...
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bioRxiv - Cell Biology 2021Quote: ... then triturated and plated on poly-L-lysine coated 8-well glass chambers (Sigma, P2636, Cellvis, C8-1-N) at a density of 25,000 cells/well in 5 % horse serum (Gibco 26050-088 ...
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bioRxiv - Cell Biology 2021Quote: ... murine CD4+ T cells were isolated and activated in a 12 well plate with culture media containing 2g/L D-Glucose-1,2-13C2 (Sigma) for 24 hours ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: ... cleaned for 30 min with 1 M KOH (Fluka, Bucharest, Romania) and coated for 30 min with 0.01 % poly-L-Lysine beforehand (P4707, Sigma). Samples were rinsed 5 times with deionized water and dried under nitrogen flow ...
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bioRxiv - Neuroscience 2019Quote: ... tissue was triturated to dissociate neurons and plated onto poly-L-lysine (30,000-70,000; Sigma-Aldrich, St. Louis, MO) coated culture dishes with Gibco neurobasal-A culture medium (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... Separation was performed using a reversed phase column (25 cm L x 10 mm D, 5 µm, Sigma-Aldrich) controlled by the LabSolutions software ...