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Citations for Millipore Sigma :
5701 - 5750 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The separated proteins were transferred by electrophoresis to polyvinylidene difluoride transfer membranes (Immobilon-P, Millipore, MA, USA). The membranes were treated with diluted affinity-purified anti-p-eIF2α (p-eIF2α ...
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bioRxiv - Biochemistry 2021Quote: ... X-ray film was exposed to the membrane following application of Immobilon Western Chemiluminescent HRP Substrate (Millipore) developing reagent ...
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bioRxiv - Synthetic Biology 2021Quote: ... Supernatant samples were separated on 10% SDS-PAGE gels and transferred to Immobilon-FL PVDF membrane (Millipore). Membranes were probed with primary antibodies ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were separated by SDS-PAGE and then transferred to a PVDF membrane (Millipore, Burlington, Massachusetts). Membranes were stained with 1:1000 dilution of Anti-SARS-CoV SΔ10 antibody ([1A9] ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were run in SDS-PAGE and transferred to Immobilon Polyscreen PVDF transfer membranes (#IPVH00010, Merck Millipore) or Amersham Protan nitrocellulose membranes (#10600001 GE Healthcare Life Sciences) ...
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bioRxiv - Cell Biology 2021Quote: ... The separated proteins were then transferred to the PVDF membranes (0.45 µm Immobilon®-E; Merck Millipore). Membranes were blocked either with 2.5% nonfat milk in TBST (50 mM Tris ...
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bioRxiv - Cell Biology 2020Quote: The indicated proteins were loaded to SDS-PAGE gel and transferred to methanol-activated PVDF membranes (Millipore), which were blocked in 5% non-fat milk containing 0.2% Tween for 1 h at room temperature (RT) ...
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bioRxiv - Genetics 2020Quote: ... membrane was divided into two halves and probed with either monoclonal anti-(c-Myc) antibody (Merck Millipore) or anti-α-tubulin antibody (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... The membranes were incubated with 1:5,000 monoclonal ANTI-FLAG M2 antibody produced in mouse (Sigma-Aldrich) and Goat Anti-Mouse IgG (H+L)∼HRP Conjugate (BioRad) ...
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bioRxiv - Immunology 2020Quote: ... IgGs were buffer exchanged into PBS by centrifugation with 30 kDa MWCO membrane centrifugal filter units (Millipore). Fabs were generated by papain digestion using crystallized papain (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was blocked in PBS-T milk 5% and probed by using anti-Flag (Sigma F1804) antibody (1:5,000 dilution) ...
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bioRxiv - Microbiology 2021Quote: ... Cell size was determined by staining cell membranes with methylene blue or Gram staining (both Sigma-Aldrich) and quantification in Fiji/ImageJ software ...
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bioRxiv - Cell Biology 2021Quote: Membrane potential was measured with 1 μM tetramethylrhodamine (TMRE, excitation: 540 nm, emission: 595 nm; Sigma Aldrich) as described before (6) ...
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bioRxiv - Molecular Biology 2021Quote: ... The upper portion of the membrane was probed with anti-HA tag antibody (mouse, Sigma-Aldrich, H3663) diluted 1:1,000 in 5% skim milk solution ...
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... proteins were separated with SDS-PAGE gels and transferred to PVDF membranes (Immobilon-P, Merck Millipore, USA). Blotted membranes were reacted with the specific primary antibodies shown in Supplementary Table 1 followed by peroxidase-linked secondary antibodies ...
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bioRxiv - Developmental Biology 2022Quote: ... of embryonic lungs were subjected to SDS-PAGE and transferred to a polyvinylidene difluoride membrane (PVDF; Millipore). Immunoblotting was performed using an anti-Nolz-1 antibody (1:1000 ...
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bioRxiv - Biochemistry 2022Quote: Protein extracts were separated by SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membranes (Millipore-Merck, Germany). Membranes were blocked with 5% non-fat milk and incubated with a specific anti-biotin antibody overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Briefly, gels were transferred in CAPS buffer (10mM CAPS, 10% ethanol) to PVDF membranes (EMD Millipore #1PVH00010) and incubated with primary antibodies at 4°C overnight (ATF4 ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was subsequently clarified using a polyethersulfone membrane filter with a 0.22 μm pore size (Millipore).
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were transferred to nitrocellulose membranes before blocking with 5% milk (Marvel) or bovine serum albumin (Sigma) w/v in PBS-T (0.1% Tween-20 in PBS) ...
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bioRxiv - Biochemistry 2022Quote: ... membranes were washed with TBS-T and incubated with goat anti-mouse IgG-peroxidase conjugate (Sigma DC02L) for 1 h at room temperature and washed again ...
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bioRxiv - Bioengineering 2022Quote: The cells on the membrane were fixed with 4% paraformaldehyde (PFA; Sigma-Aldrich, St Louis, MO, USA) for 30 minutes at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble and insoluble fractions were analyzed by SDS–PAGE and immunoblotting onto PVDF membranes (EMD Millipore) were performed using standard protocols42.
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bioRxiv - Cell Biology 2022Quote: ... Membranes were washed three times in PBST before incubation with secondary antibody (Sigma-Aldrich; GENA931 or GENA934) diluted 1:5000 in PBST containing 5% Omniblok for 1 hr at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Equivalent amounts of protein were separated by SDS-PAGE and transferred to Immobilon-P PVDF membrane (Millipore). Membranes were incubated with antibodies per manufacturer’s suggestions ...
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bioRxiv - Genetics 2022Quote: ... Membrane pieces were incubated in antibody solution {1xTBS with 1% milk and 0.2% Tween 20 (Sigma P7949)} with primary antibodies against GAPDH and CRMP2 (Supplementary Table 3 ...
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bioRxiv - Microbiology 2022Quote: ... Immunoreactivity was developed by incubating membrane filter with DAB (3,3-diaminobenzidine tetrahydrochloride) substrate (Sigma Aldrich, MO, USA) and counter-stained with hematoxylin (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was filtered through a 0.45 μm low protein binding durapore membrane (Merck Millipore Ltd., Ireland). 20 mL of the filtrate was then ultracentrifuged at 150,000 × g at 4°C for 3 h (Beckman Coulter ...
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bioRxiv - Plant Biology 2022Quote: ... proteins were electroblotted from 10% acrylamide gel to nitrocellulose membrane (Immobilon-P, MILLIPORE Corporation, Bedford, MA, USA) after the separation of SDS-PAGE ...
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bioRxiv - Plant Biology 2022Quote: ... transferred to a PVDF membrane and analyzed by immunoblot with anti-FLAG®M2-Peroxidase (HRP) (Sigma) antibody to detect TMK1-FLAG and with anti-PIN2 antibody to detect co-immunoprecipitated endogenous PIN2.
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bioRxiv - Microbiology 2022Quote: ... 3-10 mL of liquid culture was filtered through 0.2 um Polycarbonate (PC) membrane filters (Merck Millipore Ltd ...
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bioRxiv - Neuroscience 2022Quote: ... IP samples were resolved on 8%–12% SDS-PAGE and electroblotted onto Immobilon-P membranes (Merck Millipore) using a semidry transfer apparatus ...
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bioRxiv - Neuroscience 2022Quote: ... The upper portions of the membranes were incubated with MAB2166 (recognizing HTT aa 181-810, Millipore, 1;1000) along with an mTOR antibody (Cell Signaling 2972S ...
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bioRxiv - Microbiology 2022Quote: ... followed by filtration through a MF-Millipore 8 µm sterile mixed cellulose ester (MCE) membrane (Merck Millipore Ltd. ...
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bioRxiv - Molecular Biology 2022Quote: ... The membranes were then incubated with 10 ml blocking buffer (5% skim milk powder (Merck Millipore, 115363) in TBST ...
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bioRxiv - Neuroscience 2022Quote: 30 μg of homogenates were separated by SDS-PAGE and transferred to Immobilon-P Transfer Membrane (Millipore). Membranes were blocked in milk solution (5% milk in PBS and 0.1% tween 20) ...
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bioRxiv - Cell Biology 2022Quote: ... filtrated using Millex® HV membrane filter units with a pore size of 0.45 μm (Merck Millipore) and applied to a HisTrap FF column (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: The membrane lipid strips (Echelon P-6002) were blocked with 3% fatty acid free BSA (Sigma, A4612) overnight at 4°C degree ...
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bioRxiv - Plant Biology 2022Quote: ... the supernatant was taken out and filtered with 0.22 μm PVDF membrane filter Millex-GV (Merck Millipore) and samples were analyzed using the above LC-MS set-up ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were loaded on SDS-polyacrylamide gels and transferred to polyvinylidene fluoride membranes (Merck Millipore, MA, USA). Membranes were incubated in blocking solution (TBS ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were separated by SDS acrylamide gel electrophoresis and transferred to IMMOBILON-FL 26 PVDF membrane (Millipore) probed with the indicated antibodies and visualized either by chemiluminescence (according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were then transferred onto membrane inserts with prewarmed organotypic slice culture (OTC) cultured MEM-based (Sigma) medium containing 0.00125% ascorbic acid (Roth) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Microtiter plates were covered with a Breath-EasyTM sealing membrane (Sigma-Aldrich Co., St. Louis, MO, USA) and OD600 readings were recorded automatically under continuous shaking in an Elx808TM absorbance microplate reader (BioTek Instruments Inc.).
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bioRxiv - Physiology 2024Quote: ... and equal amounts were loaded for SDS-PAGE and transferred to Immobilon-Fl PVDF membrane (Millipore, IPFL00010). Each membrane was blocked for 1.5 hr at Room Temperature with Odyssey Blocking Buffer (TBS ...
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bioRxiv - Microbiology 2024Quote: ... was used as second antibody and proteins on the membrane were visualized using BCIP/NBT substrate (Sigma). When deer plasma specimens were used as primary antibody ...
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bioRxiv - Cell Biology 2024Quote: ... concentrated using Amicon Ultra-15 centrifugal filter units with an Ultracel-30 membrane (Millipore, Billerica, MA, USA), filtered through a 0.45 μM filter ...
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bioRxiv - Physiology 2024Quote: ... The lysates were then separated by SDS-PAGE and transferred to Immobilon 0.45mm membranes (Cat# IPVH00010, Millipore). Western blotting was conducted using specific various primary and secondary antibodies ...
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bioRxiv - Microbiology 2023Quote: ... The holes were covered by attaching a square of PVDF filtration membrane (0.45 µm pore size, Millipore) on the inner face of the lid using clear PVA glue (UHU ...
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bioRxiv - Microbiology 2024Quote: ... Mucus samples were pooled and filtered using nominal molecular weight limit membranes (Amicon Ultra 3K, Millipore Sigma) to remove free nutrients ...
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bioRxiv - Microbiology 2024Quote: ... Membrane was washed with Phosphate Buffer Saline (PBS) (pH 7.2) (KD Medical) Tween 20 (0.1%) (Sigma P9416) two times and once with PBS (KD Medical ...