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Citations for Millipore Sigma :
5601 - 5650 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Cells that had migrated or invaded through the membrane were stained with the crystal violet (0.5%, Sigma) after fixation with 4% PFA as described above ...
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bioRxiv - Genetics 2020Quote: ... cells were UV-irradiated (10 J/m2) through isopore polycarbonate membranes containing 3-μm-diameter pores (Millipore) and experiments were performed 2hr post-UV irradiation ...
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bioRxiv - Immunology 2020Quote: ... coated with laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane (Sigma-Aldrich, St. Louis, MO, USA) and Matrigel for transmigration experiments ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were washed three times with TBST and then incubated with horseradish peroxidase-conjugated secondary antibody (Millipore) for 1 hr at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... PVDF membranes were then washed 5 min in TBS-T (TBS and 0,1 % Tween-20, Sigma Aldrich), blocked in 5 % milk in TBS for 1 h at room temperature and incubated with the appropriate primary antibody dilutions ...
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bioRxiv - Physiology 2020Quote: ... Equal quantities of proteins were resolved by SDS-PAGE gels and blotted onto polyvinylidene difluoride membranes (Millipore). Then ...
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bioRxiv - Developmental Biology 2021Quote: ... Membranes were washed in tris-buffered saline (TBS) with 0.5% OmniPur Tween-20 (TBST; EMD Millipore, 9480) and blocked in western blocking buffer (30 g/L Bovine Serum Albumin with 0.2% NaN3 in TBST ...
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bioRxiv - Immunology 2021Quote: ... Depletion beads were packed into sterile 0.5mL centrifugal filter devices with a 0.22µm pore PVDF membrane (Millipore) and equilibrated with 3x sterile PBS washes (pH 7.2) ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were resolved on SDS-polyacrylamide gels (SDS-PAGE) and transferred onto PVDF membrane (Immobilon-P, Millipore). After being incubated with the primary antibody ...
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bioRxiv - Cell Biology 2021Quote: ... the membranes were treated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (Sigma Aldrich, 12-348) for 1 h at room temperature (RT) ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was blocked in 2% skim milk (Devondale) dissolved in phosphate buffered saline solution (PBS; Sigma) with 0.05% Tween 20 (Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... the membrane was blocked for 1 hour (PBS-T [Sigma, US] containing 5% skim milk powder [Sigma]) and incubated in 1:10,000 rabbit-anti-G ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were stripped by two 7 min incubations in stripping buffer (6 M guanidine hydrochloride (Sigma: G3272) with 1:150 β-mercaptoethanol ...
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bioRxiv - Neuroscience 2020Quote: ... separated on 4-12% Bis-Tris Mini Gel (NuPAGE) and transferred to a PVDF membrane (Millipore #ISEQ00010). Nonspecific binding was blocked for 1 hr at RT with 5% BLOTTO (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transfer to PVDF membranes and detected using either monoclonal anti-GFP (1:1000; Sigma, 11814460001), anti-HA 12CA5 (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... separated on a SDS-PAGE gel and then electrotransfered (100V, 1 hour) on a PVDF membrane (Millipore). After blocking for 1 hour in Tris-buffered saline with 0.1% Tween-20 (TBS-T ...
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bioRxiv - Biochemistry 2021Quote: The histones were separated on a 15% polyacrylamide gel and transferred onto polyvinylidene difluoride membrane (PVDF, Millipore). The membrane was blocked in blocking solution ...
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bioRxiv - Cell Biology 2021Quote: ... followed by the transfer of proteins to polyvinylidene difluoride (PVDF) membranes (EMD Millipore, catalog # IPVH00010, lot # R0HB88840).
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by SDS acrylamide gel electrophoresis and transferred to IMMOBILON-FL 26 PVDF membrane (Millipore) probed with the indicated antibodies and visualized either by chemiluminescence (according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Equivalent amounts of protein were resolved by SDS-PAGE and transferred to polyvinylidene fluoride microporous membrane (Millipore), blocked with 1.5% BSA in H20 containing 0.1% Tween-20 (TBS-T) ...
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bioRxiv - Plant Biology 2020Quote: ... Pik-1 and AVR-Pik effectors were detected probing the membrane with anti-FLAG M2 antibody (SIGMA) and anti-c-Myc monoclonal antibody (Santa Cruz) ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were labeled in 5% BSA/TBST with either anti-HA peroxidase conjugated antibodies (Sigma, 1:200) or rabbit TgALD1 antibody (1:200 ...
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bioRxiv - Synthetic Biology 2021Quote: ... the membrane was incubated with secondary antibody (1:10000) anti-Goat IgG secondary antibody (A0545, Sigma Aldrich) in the buffer (10ml 1x TBST ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were incubated for 1 hour with HRP-conjugated secondary antibodies (1:5000, anti-mouse, Sigma-Aldrich Catalogue No A9044 ...
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bioRxiv - Microbiology 2020Quote: ... Equal amounts of protein lysate were separated by SDS-PAGE and transferred onto a nitrocellulose membrane (Millipore) at 30 V for at least 60 min (overnight for ubiquitination assays) ...
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bioRxiv - Microbiology 2020Quote: ... The flow-through was collected and concentrated to approximately 5 mL using a cellulose ultrafiltration membrane (Millipore) in an Amicon ultrafiltration cell ...
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bioRxiv - Neuroscience 2020Quote: ... were loaded and separated by 10% SDS-PAGE gels and then transferred to Immobilon-P membranes (Millipore). Membranes were blocked in Tris Buffered Saline (TBS ...
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bioRxiv - Microbiology 2021Quote: ... the samples were filtered through Ultrafree-MC Hydrophilic PTFE membrane (0.2-µm pore size) spin filters (Millipore) and stored at -20 °C until measurement ...
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bioRxiv - Cancer Biology 2021Quote: ... Western blot was performed using PVDF membranes incubated with anti-vinculin (Sigma-Aldrich; V9131, 1:10,000 dilution) antibody as cytoplasmic loading control ...
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bioRxiv - Microbiology 2021Quote: ... The membrane was washed 3 times with PBS with 0.01% Tween®20 (Sigma, St. Louis, MO). Secondary antibody was incubated at room temperature protected from light for 1 hour in Intercept®(TBS ...
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bioRxiv - Neuroscience 2020Quote: ... 20 μg of proteins were separated by 10% SDS-PAGE and transferred to Immobilon-FL membranes (Millipore). For dot-blot assays ...
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bioRxiv - Microbiology 2021Quote: ... Protein samples were separated by SDS–PAGE (4-20%) and blotted onto polyvinylidene fluoride membranes (Merck Millipore). Membranes were blocked for 1 h with Tris-buffered saline (TBS ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked for 1 h with Tris-buffered saline (TBS) containing 0.1 % Tween 20 (Sigma Aldrich) and 5 % dry milk and further incubated for 1 h with primary antibodies diluted in TBS ...
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bioRxiv - Systems Biology 2021Quote: ... plates were sealed with a gas-permeable film (Breathe-easy Sealing membrane, Sigma-Aldrich, St. Louis, MO). Tryptone and yeast extract were purchased from BD Biosciences (Franklin Lakes ...
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bioRxiv - Microbiology 2020Quote: ... pH 7.2) and purified by filtration through 5 μm pore polycarbonate membranes (Merck Millipore Co. Cork, Ireland).
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bioRxiv - Microbiology 2020Quote: ... the supernatant of infected cells was concentrated through Centricon Plus-70 centrifugal filters (100,000 NMWL membrane, Millipore), then the virions were pelleted by ultracentrifugation through a 20% sucrose cushion at 38,000 rpm for 90 min ...
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bioRxiv - Cancer Biology 2020Quote: ... Blotting was performed on an Immobilin®-P PVDF membrane with 0.45 μm pore size (Merck Millipore) using a Mini Blot Module (Thermo Scientific Invitrogen™).
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bioRxiv - Cancer Biology 2020Quote: ... transferred to nitrocellulose membranes and probed with primary antibodies detecting MnSOD (Cat # 06-984, Millipore, Billerica, MA) and beta-tubulin (University of Iowa Hybridoma Core ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were blocked for 2 hours in a 2% bovine serum albumin solution (Sigma-Aldrich, MO, USA) and incubated overnight with primary antibodies PDGFRa (1:1000 ...
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bioRxiv - Biophysics 2020Quote: ... Nuclei or plasma membranes were stained in live cells after 20 min incubation with Hoechst (Sigma-Aldrich) or CellMask (Molecular Probes ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were blocked with Tris-buffered saline (TBS) containing 0.05% Tween and 5% skim milk powder (Sigma) and incubated with the primary antibody overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were blocked using 5% non-fat dry milk or 5% bovine serum albumin (BSA) (Sigma-Aldrich) for phosphorylated proteins immunoblots in Tris-buffered saline (TBS ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was blocked with tris-buffered saline containing 3% bovine serum albumin (cat. A7030, Sigma-Aldrich) and 0.1% Tween 20 (P1379 ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were subsequently incubated with peroxidase-conjugated secondary antibodies (anti-mouse IgG, HRP conjugate, EMD Millipore, AP160P ...
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bioRxiv - Biochemistry 2022Quote: ... The cell membrane was first separated from the bacteria debris via centrifugation at 6000 rpm (Sigma 3k15) for 15 mins ...
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bioRxiv - Cell Biology 2022Quote: ... Equivalent amounts of protein were resolved by SDS-PAGE and transferred to polyvinylidene fluoride microporous membrane (Millipore), blocked with 1.5% BSA in H20 containing 0.1% Tween-20 (TBS-T) ...
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bioRxiv - Neuroscience 2022Quote: ... three to four slices were transferred onto each 0.4 μm pore membrane insert (Millicell-CM, Millipore, UK). Cultures were then maintained at 37°C in 5% CO2 and fed by medium exchange every 2-3 days for a maximum of 21 days in vitro (DIV) ...
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bioRxiv - Cell Biology 2022Quote: ... Protein samples were separated in SDS-PAGE and transferred to polyvinylidene fluoride (PVDF) filter membranes (Millipore, USA) for immune-hybridization ...
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bioRxiv - Genetics 2022Quote: ... The membrane was hybridized with 5 pmol/ml of a 5’ DIG-labeled (AGAGGG)10 probe (Sigma) in DIG Easy Hybridization Solution (Sigma ...
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Construction of a constitutively active type III secretion system for heterologous protein secretionbioRxiv - Synthetic Biology 2022Quote: ... The membrane was probed with rabbit anti-GroEL according to manufacturer’s instructions (Sigma Aldrich, 1:10,000 dilution). Membranes were subsequently probed with goat anti-rabbit IgG (H+L ...