Labshake search
Citations for Millipore Sigma :
5551 - 5600 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2024Quote: ... Samples were run on 10% SDS-PAGE gels and transferred onto a PVDF membrane (Millipore, MA). Immunoblots were blocked in TBS (tris-buffered saline ...
-
bioRxiv - Immunology 2024Quote: ... Proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes (Merck-Millipore, Billerics, MA, USA) using a semi-dry transfer system (Trans Blot Transfer Medium ...
-
bioRxiv - Microbiology 2024Quote: ... membranes were incubated rocking at room temperature overnight with primary antibody: rabbit polyclonal anti-FLAG (Sigma) or mouse monoclonal anti-RpoA (Biolegend) ...
-
bioRxiv - Molecular Biology 2024Quote: Proteins were separated on a 15% SDS-Page gel and transferred to a PVDF membrane (Millipore) for 90 minutes at 100V and 4°C ...
-
The integrated stress response promotes macrophage inflammation and migration in autoimmune diabetesbioRxiv - Immunology 2024Quote: ... Membranes were incubated overnight at 4°C with primary antibodies: anti-puromycin (Millipore; MABE343; 1:1000), anti-iNOS (Novus ...
-
bioRxiv - Immunology 2024Quote: ... The extracts were separated on 12% SDS-PAGE gels and transferred to polyvinylidene difluoride membrane (Millipore). The membranes were probed with specific antibodies targeting RTF1 (Sigma) ...
-
bioRxiv - Molecular Biology 2024Quote: Samples were subjected to a standard SDS-PAGE protocol and transferred to PVDF membrane (Sigma, #IPVH00010). The membrane was blocked for 1h at room temperature using a blocking buffer (1x TBS ...
-
bioRxiv - Neuroscience 2024Quote: ... China) for separation and then transferred to a 0.2 μm PVDF membrane (Cat# 03010040001, Millipore, Germany). The PVDF membrane was blocked in TBST solution containing 5% milk powder and then incubated overnight at 4°C with diluted primary antibodies (5% milk powder TBST) ...
-
bioRxiv - Biochemistry 2024Quote: ... and the proteins were subsequently transferred to a PVDF membrane (#IPVH00010, Merck Millipore, Burlington, MA, USA). The membranes were washed with TBS-T (20 mM Tris (pH 7.5) ...
-
bioRxiv - Molecular Biology 2024Quote: ... Protein samples were transferred to nitrocellulose membranes (Amersham) upon which M2 Anti-FLAG mouse antibodies (Sigma) were used to visualize 3X-FLAG fusion peptides.
-
bioRxiv - Neuroscience 2024Quote: ... membranes were developed using Fusion Fx (Vilber Lourmat) with Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore).
-
bioRxiv - Microbiology 2024Quote: ... Porewater was filtered with 0.2 µm Durapore® PVDF membrane filters (EMD Millipore, Burlington, MA, USA).
-
bioRxiv - Microbiology 2024Quote: ... and the supernatant was filtered through (MF-Millipore™ Membrane Filter, 0.45 µm, HAWP04700 Millipore, Sigma). The filtered supernatant was treated with 10 ug/mL of TPCK trypsin (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2024Quote: ... and the supernatant was filtered through (MF-Millipore™ Membrane Filter, 0.45 µm, HAWP04700 Millipore, Sigma). The filtered supernatant was treated with 10 ug/mL of TPCK trypsin (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2024Quote: The protein samples underwent resolution through SDS-PAGE and subsequent transfer onto PVDF membranes (Millipore, MA). Subsequently ...
-
bioRxiv - Microbiology 2024Quote: ... Proteins were transferred to nitrocellulose membranes and immunoblotted with mouse anti-M2-FLAG (1:5,000) (Sigma) or mouse anti-DnaK (1:5,000 ...
-
bioRxiv - Biochemistry 2024Quote: ... samples were loaded into SDS-PAGE gels and transferred to PVDF membranes (EMD Millipore, US, IPVH00010). After blocking in 5% non-fat dry milk prepared in 1X PBS-0.1% Tween-20 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Lysates were then separated by SDS-PAGE and then transferred to PDVF-FL membrane (Millipore Sigma). Non-specific binding was blocked by incubation in 5% nonfat milk in TBS for 1 hour at room temperature ...
-
bioRxiv - Biochemistry 2024Quote: ... membrane was blocked with 5% non-fat milk in PBST or 5% BSA (Sigma-Aldrich, A9085) at RT for 1 hour ...
-
bioRxiv - Bioengineering 2024Quote: ... dialysis membrane with various molecular weight cut-offs with 3.5 kDa were purchased from Sigma Aldrich. Particle Conjugation Kit was supplied from AnteoTech company ...
-
bioRxiv - Biophysics 2024Quote: The membrane probe LAURDAN (6-dodecanoyl-2-dimethylamino naphthalene; Sigma-Aldrich, St. Louis, MO-USA, # 40227) was dissolved in DMSO to make a stock concentration of 3.1 mM ...
-
bioRxiv - Biophysics 2024Quote: ... Total of loading protein ware visualized by staining of protein-blotted membrane with ponceau solution (Sigma). Membranes were probed with anti-PPARγ antibody (CST ...
-
bioRxiv - Cell Biology 2024Quote: ... the fractionated proteins in the gel were transferred to a polyvinylidene difluoride (PVDF) membrane (IPVH00010; Millipore) by a semi-dry blotter (BE-320 ...
-
bioRxiv - Cell Biology 2024Quote: ... Samples were separated by SDS-polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride (PVDF) membranes (Millipore) followed by blocking with 5% BSA in Tris-buffered saline with 0.05% Tween-20 ...
-
bioRxiv - Cell Biology 2024Quote: ... Proteins were separated on 10% polyacrylamide gels and transferred onto 0.45 μm nitrocellulose membranes (Sigma-Aldrich). Membrane blocking was performed in 2% BSA in PBS for 30 minutes at room temperature ...
-
bioRxiv - Bioengineering 2024Quote: ... polydopamine-coated nanofibrous membranes were immersed in 3 mg/mL gelatin type B (Sigma Aldrich, Germany) solution in 10 mM Tris-HCl buffers ...
-
bioRxiv - Biochemistry 2024Quote: ... and then proteins were resolved by SDS–PAGE and transferred to PVDF membranes (Immobilon-P; Millipore). Primary antibodies used include mouse anti-phospho-p44/42 (1:1000 ...
-
bioRxiv - Cell Biology 2024Quote: ... Membranes were washed three times in 1X TBST (1X TBS with 0.1% Tween 20; Sigma P9416), then incubated for one hour at room temperature while rocking in secondary antibody diluted in 2% milk ...
-
bioRxiv - Cell Biology 2024Quote: ... the membrane was incubated with an anti-protein A (Sigma, P3775; used at 1:10,000 dilution) or anti-V5 antibody (Merck ...
-
bioRxiv - Cell Biology 2024Quote: Whole cell lysates were run on SDS-PAGE gels and transferred onto a PVDF membrane (Millipore). Non-specific binding was blocked with 5% skim milk in Tris-buffered saline with 0.1% Tween-20 (TBS-T) ...
-
bioRxiv - Developmental Biology 2024Quote: ... Nitrocellulose membranes (Cytiva, 10600002) were incubated overnight with the following antibodies: FLAG (Sigma, F1804; 1:1,000), ARHGEF3 (ThermoFisher ...
-
bioRxiv - Microbiology 2022Quote: ... or manual extraction kits (Invitrogen PureLink genomic DNA kit or the Sigma GenElute kit). Normalised DNA (at a concentration of 0.2 ng/ml ...
-
bioRxiv - Plant Biology 2024Quote: ... Kit (Sigma). RNA was treated with TURBO DNAse (Thermo ...
-
bioRxiv - Cancer Biology 2021Quote: ... The membrane was washed with TBST then incubated with appropriate horseradish peroxidase-conjugated secondary antibody (Sigma-Aldrich, Anti-mouse CAT# A4416 and Anti-Rabbit #A6154 ...
-
bioRxiv - Cancer Biology 2021Quote: ... 50 µg proteins were separated by SDS-PAGE and transferred onto polyvinylidene difluoride membrane (PVDF, Millipore, IPVH00010). The membranes were incubated in blocking buffer (5% w/v nonfat dry milk in Tris-buffered saline ...
-
bioRxiv - Cancer Biology 2021Quote: ... Membranes were probed with primary antibodies against AXIN1 (2087, CST), TCF4 (2569, CST) pFAK Y397 (SAB4504403, Sigma), DUSP6 (EPR129Y ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were separated by 8% to 12% SDS–PAGE and transferred to Immobilon-P PVDF membrane (Millipore). Blocking and probing with antibodies were performed as previously described (Iréna Lassot et al. ...
-
bioRxiv - Cell Biology 2020Quote: ... Nuclear envelope breakdown was scored by observation of intact follicles cultured on organotypic membranes (Millipore, Cork IR) such that the prophase arrested nucleus (germinal vesicle ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins (25-50µg) were resolved on 8 to 15% SDS-PAGE and blotted onto PVDF membrane (Millipore). Membranes were blocked for 1 h at room temperature with TBS-0.1% Tween20 and 5% milk or 1% bovine serum albumin (BSA) ...
-
bioRxiv - Developmental Biology 2021Quote: ... Protein lysates were resolved by SDS/PAGE under reducing condition and transferred to PVDF membranes (Merck Millipore). After blocking ...
-
bioRxiv - Genetics 2021Quote: ... Membrane was then washed again in PBST and left 30 minutes in Ponceau S Solution (Sigma-Aldrich) with agitation before a last water washing.
-
bioRxiv - Immunology 2020Quote: ... then 25 ug total protein was separated by 10% SDS-PAGE and transferred to PVDF membranes (Millipore). Membranes were blocked with 5% non-fat milk and probed with the indicated primary antibodies following the manufacturer’s instruction ...
-
The key features of SARS-CoV-2 leader and NSP1 required for viral escape of NSP1-mediated repressionbioRxiv - Molecular Biology 2021Quote: ... The membrane was probed with the following primary antibodies: mouse anti-flag antibody 1:2000 (F1804 Sigma), mouse anti-beta-actin 1:5000 (A2228 Sigma).
-
bioRxiv - Biophysics 2020Quote: ... Separated proteins were transferred to a nitrocellulose membrane and immunoblotted with an antibody recognizing Orai1 (Sigma Aldrich). Each experiment was performed at least 3 independent times and analysed with the program ImageJ (NIH ...
-
bioRxiv - Developmental Biology 2021Quote: ... Equal amounts of protein were run on SDS-polyacrylamide gel and transferred to Immobilon-P membrane (Millipore). Membranes were incubated with anti-YAP (Novus) ...
-
bioRxiv - Physiology 2022Quote: ... Membranes were blocked for 1 hour at room temperature in 5% milk or 5% BSA (Millipore Sigma) in 1X Tris-buffered saline + 0.1% Tween 20 (TBST) ...
-
bioRxiv - Molecular Biology 2020Quote: ... proteins were transferred to a polyvinylidene difluoride (PVDF) membrane (Immobilon, 0.2 μm pore, Millipore Corp., MA, USA) and incubated overnight at 4°C with primary antibody solution containing polyclonal rabbit anti-mouse ApoA-II antiserum (diluted 1:3000 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Samples were separated by SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membranes (Millipore Immobilon-FL, #IPVH00010) for immunoblotting ...
-
bioRxiv - Molecular Biology 2020Quote: ... The membranes were probed with a secondary antibody: either anti-rabbit-HRP (1:5,000; Merck Millipore AP132P), anti-mouse-HRP (1:5,000 ...
-
bioRxiv - Immunology 2021Quote: ... Membranes were washed thoroughly with TBS-Tween (0.1%) after antibody incubations and developed using ECL reagents (Millipore). For re-probing blots were stripped for 30 min at 37 °C with Restore PLUS stripping buffer (Thermo Scientific).