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Citations for Addgene :
1701 - 1750 of 1797 citations for Penicillin G since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... cells were transfected with a combination of three plasmids: 5 μg of pMD2.G (gift from Didier Trono, Addgene plasmids #12259), 15 μg of psPAX2 (gift from Didier Trono ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids were then linearized with MluI and 2μg of plasmid was transfected into 5×105 HEK293 cells together with a plasmid encoding the T7 polymerase 63 (Addgene 65974) using calcium phosphate ...
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bioRxiv - Cell Biology 2020Quote: Plasmids used for transfection include pEGFP-C1 (GenBank: U55763.1, Clonetech) and pcDNA3-EGFP-Rac1-Q61L (a gift from G. Bokoch, Addgene plasmid #12981) (Subauste et al. ...
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bioRxiv - Microbiology 2022Quote: GFP and myc-tagged 14-3-3ε were generated as follows: a G-block containing the full-length 14-3-3ε protein (IDT) was cloned into pEGFP-C1 (Addgene #2487) using XhoI and BamHI restriction sites ...
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bioRxiv - Cancer Biology 2022Quote: ... 293T cells were transfected with retroviral or lentiviral transfer plasmid and packaging vector (retrovirus: pCL-Eco, Addgene, 12371; lentivirus: psPAX2, Addgene, 12260 with VSVg envelop plasmid pMD2.G, Addgene, 12259) using Mirus TransIT-LT1 (Mirus ...
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bioRxiv - Cancer Biology 2022Quote: ... Viral supernatants were prepared by transfection of HEK293T cells with the vector plasmids and packaging plasmids (psPAX2 Addgene 12259 and pMD2.G Addgene 12260). The transfection was performed with polyethylenimine (PEI MAX Transfection Grade Linear Polyethylenimine Hydrochloride ...
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bioRxiv - Cancer Biology 2022Quote: ... The lenti-vectors expressing each pair of gRNAs targeting distal enhancers were packaged in 293T cells using pMD2.G (Addgene # 12259) and psPAX (Addgene # 12260) ...
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bioRxiv - Genomics 2022Quote: ... Viral particles were produced by transient transfection of HEK293T cells with the library and packaging plasmids pMD2.G (Addgene no. 12259) as well as psPAX2 (Addgene no ...
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bioRxiv - Microbiology 2022Quote: Lentiviral particles were generated by co-transfection of the expression constructs and 2nd generation packaging plasmids (psPAX2, Addgene#12260 & pMD2.G, Addgene#12259), using jetPEI DNA transfection reagent (Polyplus transfection ...
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bioRxiv - Neuroscience 2023Quote: ... pLVX-UbC-rtTA-Htt-Q94-CFP vector was co-transfected in HEK293T cells using Lipofectamine2000 with packaging vectors pMD2.G (Addgene, #12259) and psPAX2 (Addgene ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293T cells were transfected with targeting plasmid and 3rd generation packing plasmid (pMD2.G, pMDLg/pRRE; Addgene #12251, and pRSV-Rev; Addgene #12253) together using Lipofectamine 2000 ...
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bioRxiv - Genomics 2024Quote: ... 4.5 x 106 cells were transfected using the calcium phosphate precipitation method (Salmon and Trono, 2007) with 6 μg pMD2.G (Addgene #12259), 15 μg psPAX2 (Addgene #12260 ...
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bioRxiv - Cancer Biology 2023Quote: ... Transfer lentiviral plasmids (2500 ng) were diluted in 200 µl serum-free medium together with 225 ng pCMV-VSV-G (Addgene #8454) and 2250 ng psPAX2 (Addgene #12260 ...
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bioRxiv - Cancer Biology 2023Quote: Lentiviruses and retroviruses were produced by co-transfecting the transfer plasmids with pMD2.G (Addgene #12259, a gift from Didier Trono) and psPAX2 (Addgene #12260 ...
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bioRxiv - Cell Biology 2023Quote: ... The following day, cells were co-transfected (using Lipofectamine 3000 treatment, as described above) with pMD2.g (envelope plasmid, Addgene #12259), psPAX2 (packaging plasmid ...
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bioRxiv - Cell Biology 2023Quote: Lentiviral particles were prepared in HEK293T cells using standard calcium-phosphate transfection of the lentivector with packaging plasmids pMD2.G (Trono lab, Addgene #12259) and pCMV-dR8.74 (Trono lab ...
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bioRxiv - Cell Biology 2023Quote: ... Transfection was performed using 1100 ng of total DNA (500 ng transfer plasmid, 500 ng pCMVR8.74 Addgene plasmid # 22036, 100 ng pCMV-VSV-G Addgene plasmid # 8454) The day before transfection ...
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bioRxiv - Microbiology 2023Quote: ... lentiviral particles pseudotyped with the VSV-G protein were produced by cotransfecting HEK293T cells in 10cm dishes with 5 mg pLentiCMVPuroDEST vector (Addgene, #17452), 2 mg VSV-G Env expression vector pMD2.G (Addgene #12259 ...
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bioRxiv - Cancer Biology 2023Quote: ... pMD2.G was a gift from Didier Trono (Addgene plasmid # 12259; http://n2t.net/addgene:12259; RRID:Addgene_12259; accessed on 22 March 2021).
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bioRxiv - Cell Biology 2023Quote: ... at roughly 80% confluency with 4.5μg of an equimolar mixture of three plasmids encoding the envelope and viral packaging components from the laboratory of Didier Trono (pMD2.G: Addgene 12259; pRSV-Rev: Addgene 12253; pMDLg/pRRE: Addgene 12251). 4.5μg of lentiviral donor plasmid was added to the mixture and incubated for 20 min with 10μL of polyethylenimine (PEI ...
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bioRxiv - Neuroscience 2023Quote: ... The pSAD 11.G H2B:GFP was obtained by taking advantage of the pSAD 11.G F3 expression vector (kindly provided by M. Tripodi, Cambridge University, AddGene #32634). The nucleotide sequence encoding the histone H2B-tagged GFP was designed and ordered via GeneArts (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... Lentiviral particles for cas9 and HSP90-alpha were produced using HEK293T cells by con-transfection of transgene plasmid and pMD2.G (Addgene# 12259) and psPAX2 (Addgene #12260) ...
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bioRxiv - Microbiology 2023Quote: ... two 6-well plates of 293T cells were transfected with plasmid pools described above, lentivirus helper plasmids (BEI: NR-52517, NR-52519, NR-52518) and VSV-G expression plasmid (Addgene #204156). This produced a VSV-G pseudotyped lentivirus pool carrying mutagenised spike sequences in their genomes ...
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bioRxiv - Genomics 2023Quote: ... Lentivirus containing the landing pad sequence was produced by co-transfecting 293T cells at ∼50% confluence with psVSV-G (Addgene #12259), psPAX2 (Addgene #12260) ...
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: Lentivirus was produced via transfection of library plasmid pool and appropriate packaging plasmids (psPAX2, Addgene #12260 and pMD2.G, Addgene #12259) using linear polyethylenimine MW25000 (Polysciences ...
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: Lentivirus was produced via transfection of library plasmid pool and appropriate packaging plasmids (psPAX2, Addgene #12260 and pMD2.G, Addgene #12259) using linear polyethylenimine MW25000 (Polysciences ...
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bioRxiv - Microbiology 2023Quote: ... lentiviral particles expressing the above genes were produced by co-transfecting expression plasmids individually with a 2nd generation lentiviral packaging construct psPAX2 (courtesy of Dr Didier Trono through NIH AIDS repository) and VSVG plasmid pMD2.G (Addgene, 2259) in HEK293T producer cells using polyethyleneimine as previously described 54.Virus supernatant was collected 72 h post-transfection ...
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bioRxiv - Developmental Biology 2023Quote: ... lentiviral supernatants were generated by transfecting one 10-cm plate of HEK-293T cells at 90% confluency with 3 µg pMD2.G (Addgene #12259), 9 µg psPAX2 (Addgene #12260) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Lentiviral particles containing the CD19 CAR element were produced by lipofectamine-based co-transfection of HEK293 cells with 3rd generation packaging plasmids pMD2.G (Addgene, #12259), pMDLg/pRRE (Addgene ...
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bioRxiv - Immunology 2024Quote: ... Lentivirus was produced using standard virus production methods by co-transfecting target and packaging plasmids (psPAX2 – Addgene12260 and pMD2.G-Addgene 12259) into HEK293T cells ...
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bioRxiv - Cancer Biology 2024Quote: Lentivirus particles were generated in HEK293T cells following transfection with the CAR plasmid along with pMD2.G envelope (Addgene, Cat.12259) and psPAX2 packaging plasmids (Addgene ...
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bioRxiv - Neuroscience 2024Quote: ... University of Memphis) and packaging plasmids psPAX2 and pMD2.G (gift from Didier Trono (Addgene plasmid # 12260 and 12259; RRID:Addgene_12260 and 12259)) ...
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bioRxiv - Cancer Biology 2021Quote: ... or the third-generation EGFP transfer plasmid pHAGE-CMV-EGFP-W (EvNO00061634, Harvard Plasmid Repository) were mixed with viral envelope plasmid pMD2.G (12259, Addgene, Watertown, MA) and viral packaging plasmid psPAX2 (12260 ...
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bioRxiv - Biochemistry 2021Quote: ... The lentivirus envelope and packaging plasmids pMD2.G and pCMV delta R8.2 were gifts from Didier Trono (Addgene plasmids # 12259 and 12263). Plasmids containing human TIM-1 and human TIM-4 were obtained from Origene ...
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bioRxiv - Molecular Biology 2021Quote: VSV-G envelope pseudo-typed lentivirus for the generation of stable cell lines was produced by co-transfection of each transfer plasmid with pCMVdR 8.91 71 and pCMV-VSV-G (gift from Prof. Weinberg, Addgene plasmid # 8454 72). 72 h post-transfection ...
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bioRxiv - Developmental Biology 2022Quote: HEK293T cells were grown in 10-cm dishes to 80% confluency before transfection with the lentiviral vector (10 µg) with packaging vectors including pMD2.G (3 µg, Addgene plasmid # 12259), psPAX2 (6 µg ...
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bioRxiv - Molecular Biology 2019Quote: An N-terminal FLAG tag sequence was appended via Gibson Assembly Cloning (New England Biosciences) to a human codon optimized Cas9 (subcloned from hCas9, a gift from G. Church, Harvard; Addgene plasmid 41815) with a single C-terminal NLS expressed from a pcDNA3.3-TOPO vector ...
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bioRxiv - Developmental Biology 2021Quote: We grew HEK293T cells in 10-cm dishes to a confluence of 80% before we transfected the lentiviral vector (10 µg) with packaging vectors including pMD2.G (3 µg, Addgene plasmid # 12259), psPAX2 (6 µg ...
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bioRxiv - Cell Biology 2021Quote: ... HEK 293 cells were transfected with pLJM1-eGFP or pLJM1-DN-FYN plasmid together with lentivirus packing and envelope plasmids pMD2.G (gift from Didier Trono, Addgene plasmid# 12259) and psPAX2 (gift from Didier Trono ...
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bioRxiv - Biochemistry 2021Quote: Lentiviral expression constructs were packaged in HEK293FT cells using calcium phosphate transfection of psPAX2 and pMD2.G (kind gifts of D. Trono; Addgene #12260, 12259) and pTAT (kind gift of P ...
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bioRxiv - Biophysics 2022Quote: ... media was refreshed with standard growth media and cells were co-transfected with SNAP-mGluR2 (no FLAG-tag) and chimeric G protein (Gqo5, Addgene plasmid #24500) (1:2 w/w ...
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bioRxiv - Plant Biology 2022Quote: ... the CP or MP fragments were cloned into vector L4440gtwy that includes attR sites flanked by T7 promoters (G. Caldwell, Addgene plasmid # 11,344) using LR clonase (Thermo Scientific ...
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bioRxiv - Immunology 2020Quote: ... the lentiCas9-v2 lentivirus were produced from HEK-293FT cells transfected with the lentiCas9-v2 plasmid mixed at a 2:1:1 DNA ratio of the lentiviral packaging plasmids pMD2.G (Addgene plasmid #12259) and psPAX2 (Addgene plasmid #12260 ...
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bioRxiv - Genomics 2019Quote: ... were seeded in a 12-well plate and cultured for 24 h before transfection with Sp1-luciferase reporter plasmid DNA (0.5 g; Panomics, Fremont, CA, USA) or a 3× ERE TATA luc construct (Addgene, Cambridge, MA, USA) for 24 h ...
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bioRxiv - Cancer Biology 2021Quote: Oligos targeting p53 were annealed and inserted into lentiCRISPR v2 (gift of Feng Zhang) as described previously (Sanjana et al., 2014; Shalem et al., 2014) The lentiviral packaging plasmids pMD2.G (Addgene plasmid #12259) and psPAX2 (Addgene plasmid #12260 ...
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bioRxiv - Immunology 2021Quote: Lentiviruses were generated by co-transfection of lentiCRISPR v2 constructs and packaging plasmids (psPAX2, Addgene no. 12260 and pMD2.G, Addgene no. 12259), using PEI DNA transfection reagent (Shanghai maokang biotechnology) ...
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bioRxiv - Cancer Biology 2022Quote: ... Lentivirus was generated using 293FT cells by co-transfection of guide plasmid with psPAX2 and pMD2.G (Addgene, 12260 and 12259, respectively). Conditioned media was harvested ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmid psPAX2 was a gift from Didier Trono (Ad-dgene plasmid #12260) and pMD2.G was a gift from Didier Trono (Addgene plasmid #12259). A7R5 cells were induced towards the contractile phenotype using serum starvation ...
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bioRxiv - Genomics 2022Quote: Lentiviral particles were produced by transfecting 293T-17 cells (ATCC: CRL-11268) with the envelope construct pCMV-VSV-G (Addgene plasmid 8454), the packaging construct pCMV-dR8.2 dvpr (Addgene plasmid 8455) ...
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bioRxiv - Developmental Biology 2022Quote: ... HEK293T cells at 70-80% confluency in 10 cm dishes were transfected with the insert construct plus 3rd generation packaging plasmids: pMD2.G (3 μg, Addgene plasmid #12259), psPAX2 (6 μg ...