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Citations for Addgene :
1501 - 1550 of 1797 citations for Penicillin G since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: ... envelope vector pMD2.G (ID #12259) and scrambled shRNA plasmid (sc: ID1864) were obtained from Addgene (Cambridge, MA). HEK293T cells in 10 cm dishes were transfected using 50µL 0.1% Polyethylenimine ...
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bioRxiv - Synthetic Biology 2020Quote: Lentivirus production was performed using HEK-293FT cells and second-generation helper plasmids MD2.G (Addgene plasmid #12259) and psPax2 (Addgene plasmid #12260) ...
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bioRxiv - Bioengineering 2020Quote: ... Plasmids pMD2.G and pCMVR8.74 were gifts from Didier Trono (Addgene plasmid #12259 and Addgene plasmid #22036, respectively). HEK293T cells were then infected in order to establish polyclonal stable cell lines via the integration of the lentiviral genetic elements into the cell genome ...
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bioRxiv - Synthetic Biology 2020Quote: ... Plasmids pMD2.G and pCMV-dR8.2 dvpr were a gift from Didier Trono (Addgene plasmid # 12259 and 8455).
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bioRxiv - Cell Biology 2019Quote: ... VSV-G pseudotyped lentiviral particles were packaged using a third generation packaging system3 (Addgene plasmids # 12251, 12253, 12259). HeLaK or RPE1 cells were then transduced with low virus titers (MOI ≤1 ...
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bioRxiv - Cell Biology 2019Quote: ... were co-transfected with pLVX-TDP-43 along with the lentiviral packaging vectors pCMV-VSV-G (Addgene #8454), and pSPAX2 (Addgene #12260 ...
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bioRxiv - Cancer Biology 2020Quote: HEK293T cells were transfected with packaging plasmid psPAX2 and envelope plasmid pMD2.G (both gifts from Didier Trono, Addgene plasmid # 12260; http://n2t.net/addgene:12260; RRID:Addgene_12260 and Addgene plasmid # 12259 ...
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bioRxiv - Cancer Biology 2022Quote: ... with 6μg of the plasmid of interest and 6μg of the packaging plasmids pCMV-VSV-G (Addgene, 8454) and pCMVdR8.2 dvpr (Addgene ...
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bioRxiv - Cancer Biology 2022Quote: ... and the resulting pQCXIN-PD-L1-IRES-Thy1.1 plasmid was transfected into HEK293T cells along with pCMV-VSV-G (a gift from Bob Weinberg; Addgene plasmid 8454; http://n2t.net/addgene:8454; RRID:Addgene_8454) and pCL-Eco (a gift from Inder Verma ...
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bioRxiv - Genomics 2022Quote: ... 5 μg DNA was incubated with1.75 μg pMD2.G and 3.25 μg pCMV-dR8.91 (Addgene, Watertown, MA; #12259). On the next day ...
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bioRxiv - Genomics 2022Quote: ... pCMV-VSV-G was a gift from Bob Weinberg (Addgene plasmid # 8454; http://n2t.net/addgene:8454; RRID: Addgene_8454). Prior to transfection ...
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bioRxiv - Microbiology 2022Quote: ... HEK293T cells were used to package lentiviruses by co-transfecting viral packaging plasmids pCMV-VSV-G (Addgene #8454), psPAX2 (Addgene #12260 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The hACE-P2A-Puro gene encoding VSV-G lentiviral particles were produced by second generation lentiviral plasmids (Addgene) and transfected both pLenti-hACE-P2A-Puro ...
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bioRxiv - Molecular Biology 2024Quote: ... the HEK293T cells were transfected with the respective constructs along with envelope plasmid (pMD2.G) (Addgene, Cat# 12259), and packaging plasmid (psPAX2 ...
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bioRxiv - Molecular Biology 2024Quote: ... and pCMV-VSV-G (Stewart et al., 2003) (a gift from Bob Weinberg (Addgene plasmid # 8454 ; http://n2t.net/addgene:8454 ; RRID:Addgene_8454)) into LentiX-cells (Takara ...
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bioRxiv - Molecular Biology 2023Quote: ... VSV-G pseudotyped lentiviral particles were packaged using a third-generation packaging system (Addgene plasmids 12251, 12253, 12259).
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bioRxiv - Cancer Biology 2023Quote: ... 4×105 HEK 293T cells were transfected with pCDH-EF1-sCTLA-4 expression plasmid (1.5 µg) and psPax2 (2 µg) and pMD2.G (1.5 µg) packaging plasmids (Addgene), using Viafect™ (Promega ...
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bioRxiv - Genetics 2023Quote: ... 3.88 µg of the plasmid pCMV-VSV-G (a gift from Bob Weinberg (Addgene plasmid #8454; http://n2t.net/addgene:8454; RRID:Addgene_8454)) (103) ...
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bioRxiv - Cell Biology 2023Quote: ... The lentiviral packaging plasmids pMD2.G and psPAX2 (Didier Trono; 12259, 12260) were obtained from Addgene (Cambridge, MA). Four 15-cm culture plates containing HEK293T cells at 80% confluency were each transfected with 8 μg pTRIPZ-OGT-FH ...
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bioRxiv - Bioengineering 2023Quote: ... viral envelope (pMD2.G was a gift from Didier Trono; Addgene plasmid #12259; http://n2t.net/addgene:12259; RRID:Addgene_12259). At 48 and 72 h post transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... and pCMV-VSVG plasmid (pMD2.G) were kind gifts from Feng Zhang15 (Addgene plasmid #62988; https://www.addgene.org/62988/; RRID: Addgene_62988) and Didier Trono (Addgene plasmid #12259 ...
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bioRxiv - Genetics 2023Quote: ... Viruses were produced according to standard procedures using commercially available plasmids (pMD2.G Addgene #12259, psPAX Addgene #12260). Briefly ...
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bioRxiv - Genetics 2023Quote: ... Viruses were produced according to standard procedures using commercially available plasmids (pMD2.G Addgene #12259, psPAX Addgene #12260). Briefly ...
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bioRxiv - Genetics 2023Quote: ... 3 µg of the plasmid pCMV-VSV-G (a gift from Bob Weinberg (Addgene plasmid #8454; http://n2t.net/addgene:8454; RRID:Addgene_8454)) (103 ...
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bioRxiv - Biochemistry 2023Quote: ... and psPAX2 and pMD2.G packaging systems (gracious gifts from Didier Trono: Addgene plasmids 12260 and 12259 respectively) were eluted into TE elution buffer ...
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bioRxiv - Cell Biology 2023Quote: ... the second generation viral packaging plasmids psPax2 (Plasmid #12260) and pMD2.G (Plasmid #12259) were purchased from Addgene. To generate viral particles containing the DNA for a desired construct ...
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bioRxiv - Biochemistry 2023Quote: ... The pVSV-G plasmid was a gift from Akitsu Hotta (Addgene plasmid #138479; http://n2t.net/addgene:138479; RRID:Addgene_138479) (46) ...
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bioRxiv - Bioengineering 2023Quote: ... followed by addition of the plasmid cocktail containing 4 µg of pMD2.G (a gift from Didier Trono; Addgene plasmid # 12259; http://n2t.net/addgene:12259; RRID:Addgene_12259), 10 µg of psPAX2 (a gift from Didier Trono ...
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bioRxiv - Microbiology 2023Quote: ... with plasmids pCMV-VSV-G (a gift from Bob Weinberg Addgene plasmid # 8454 ; http://n2t.net/addgene:8454 ; RRID:Addgene_8454), BaEVRLess (gifted by Els Verhoeyen ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentiviral particles were generated by co-transfecting the respective vectors with the packaging plasmids pMD2.G (RRID: Addgene_12259) and psPAX2 (RRID ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentivirus was generated by transfecting the PIKFYVE-containing plasmid with the packaging plasmids pMD2.G and psPAX2 (Addgene 12259 and 12260 ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentiviral packaging plasmid psPAX2 and envelope plasmid pMD2.G were gifts from Didier Trono (Addgene plasmids #12260, #12259)
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bioRxiv - Bioengineering 2023Quote: ... Lentiviruses were packaged using 293FT cells by co-transfection of lentiviral transfer plasmids with pMD2.G (Addgene #12259) and psPAX2 (Addgene #12260 ...
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bioRxiv - Immunology 2023Quote: ... and 5 ug pCMV-VSV-G (kind gift from Bob Weinberg (Addgene plasmid #8454; http://n2t.net/addgene:8454; RRID:Addgene_8454) using Lipofectamine 3000 (Invitrogen) ...
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bioRxiv - Genomics 2024Quote: ... and pMD2.G (psPAX2 and pMD2.G were a gift from Didier Trono, Addgene plasmid # 12260 ; http://n2t.net/addgene:12260 ; RRID:Addgene_12260) were obtained from Addgene ...
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bioRxiv - Neuroscience 2024Quote: ... constructs to make lentivirus (pMD2.G and psPAX2) were gifts of Didier Trono (Addgene plasmid # 12259; http://n2t.net/addgene:12259; RRID:Addgene_12259 ...
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bioRxiv - Molecular Biology 2024Quote: ... and the lentiviral packaging plasmid pCMV-VSV-G a gift from Bob Weinberg (Addgene plasmid #8454; http://n2t.net/addgene:8454; RRID:Addgene_8454) (Stewart et al. ...
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bioRxiv - Microbiology 2024Quote: ... and pCMV-VSV-G was a gift from Bob Weinberg (Addgene plasmid # 8454 ; http://n2t.net/addgene:8454 ; RRID:Addgene_8454). The same approach was used to generate lentiviruses expressing UL12.5-SPA (PGK-UL2.5-SPA) ...
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bioRxiv - Physiology 2021Quote: ... 100 U/ml penicillin and 100 ug/ml streptomycin in a 5% CO2/95% air atmosphere at 37C Plasmid GP-CMV-GCaMP6s (Addgene plasmid # 40753) was cloned into lentiviral vector pCDH-EF1-MCS-IRES (puro ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 cells were transfected with 4µg pSMPUW-IRIS-Neo-H2BmRFP (Fachinetti Lab) + 4µg pMD2.G (12259 from Addgene, RRID:Addgene_12259) + 4µg psPAX2 (12260 from Addgene ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were transfected with the addressing and packaging plasmids (pCMV delta R8.2, Addgene #12263; pCMV-VSV-G, Addgene #8454). After 48 hours of expression ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were transfected with the addressing and packaging plasmids (pCMV delta R8.2, Addgene #12263; pCMV-VSV-G, Addgene #8454). After 48 hours of expression ...
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bioRxiv - Genomics 2022Quote: ... Per transfection reaction we used 7.5ul of 1 mg/mL polyethyleneimine (PEI) and 2.325 μg of total plasmid DNA (825 ng psPAX2: Addgene #12260; 550 ng pMD2.G: Addgene #12259 ...
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bioRxiv - Genomics 2019Quote: Lentivirus was produced via transfection of library plasmid with appropriate packaging plasmids (psPAX2: Addgene 12260; pMD2.G: Addgene 12259) using polyethylenimine (PEI ...
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bioRxiv - Genomics 2019Quote: Lentivirus was produced via transfection of library plasmid with appropriate packaging plasmids (psPAX2: Addgene 12260; pMD2.G: Addgene 12259) using polyethylenimine (PEI ...
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bioRxiv - Cell Biology 2019Quote: ... Lentivirus carrying CRISPR-Cas9 constructs were produced using HEK293 cells with the following plasmids: pSS172 (pMD2.G, Addgene #12259), pSS173 (psPAX2 ...
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bioRxiv - Immunology 2021Quote: ... The DNA of the constructs was mixed with the lentiviral envelope and packaging vectors pMD2-G and psPAX2c (Addgene) and polyethylenimine (PEI ...
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected with 1 μg pLentiCMV DEST plasmid + 0.25 mg of each of three 3rd generation lentiviral packaging plasmids (pMDLg/pRRE, pRSV-Rev and pMD2.G, Addgene plasmids #12251 ...
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bioRxiv - Cell Biology 2021Quote: The lentiviral packaging plasmid psPAX2 and the VSV-G envelope plasmid pMD2.G were gifts from Didier Trono (psPAX2: Addgene plasmid #12260; http://n2t.net/addgene:12260; RRID: Addgene_12260 ...
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bioRxiv - Cell Biology 2021Quote: ... The plasmids were then co-transfected separately with third-generation lentiviral packaging and envelope vectors: pMD2.G (Addgene #12259), pRSV-Rev (Addgene #12253) ...