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Citations for Addgene :
9301 - 9350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... cells were co-transfected with 900 ng of the I-SceI plasmid (pCBASceI, Addgene #26477) and 900 ng of pcDNA4/TO-HHV-6B IE1 (+I-SceI ...
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bioRxiv - Genetics 2023Quote: ... and 2.5 μg pMD2.G (Addgene plasmid #12259) using FUGENE HD (Promega E2311) ...
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bioRxiv - Genetics 2023Quote: ... 7.5 μg PAX2 (Addgene plasmid #12260), and 2.5 μg pMD2.G (Addgene plasmid #12259 ...
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bioRxiv - Neuroscience 2023Quote: ... and a cre-dependent virus (AAV9-DIO-Ef1a-eYFP, Addgene #27056) were used.
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bioRxiv - Immunology 2023Quote: ... or mScarlet (Addgene 85044) was cloned into pLV-CMV-MCS-IRES-Puro-Sin (66 ...
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bioRxiv - Neuroscience 2023Quote: ... A virus lacking the hM4Di DREADD gene and instead containing the fluorescent tag eGFP (AAV8-CaMKIIa-EGFP, packaged by Addgene) was infused into ACC in 4 animals as a null virus control ...
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bioRxiv - Neuroscience 2023Quote: AAV1 pAAV.CAG.LSL.TdTomato (Addgene 100048)
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bioRxiv - Neuroscience 2023Quote: ... AnkG-mCherry was a gift from Benedicte Dargent (Addgeneplasmid #42566; http://n2t.net/addgene:42566; RRID: Addgene_42566). HA-ZDHHC5 and HA-ZDHHC8 were kind gifts from Dr ...
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bioRxiv - Cell Biology 2023Quote: ... the nucleotide sequence of pEGFP-Mieap between the Nhe I and Xho I restriction sites containing EGFP was replaced with nucleotide sequence of pTagRFP-T-EEA1 (Addgene #42635) between the Nhe I and Xho I restriction sites containing TagRFP-T ...
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bioRxiv - Cell Biology 2023Quote: ... Ad-mApple-TOMM20 derived from mApple-TOMM20-N-10 (Addgene #54955), Ad-EGFP-BNIP3 ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmid HaloTag-KEAP1 (a gift from Yimon Aye, plasmid # 58240) was purchased from Addgene. HiBit-CDK9 were constructed through the Gibson assembly method ...
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bioRxiv - Cancer Biology 2023Quote: The plasmid pet28a-His6-Keap1 for expressing His-KEAP1 was purchased from Addgene (a gift from Yimon Aye ...
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bioRxiv - Cancer Biology 2023Quote: ... The template plasmids HA-CDK9 (a gift from Andrew Rice, plasmid # 28102) and V5-TurboID-NES_pCDNA3 (a gift from Alice Ting, plasmid #107169) were purchased from Addgene. The primer pair (5′- cagtctgcggtctgccgaaaagctgcagATGGCAAAGCAGTACGACTCGGTGGAGT-3′ and 5′-cagggtcaggcgctccaggggaggcagTCAGAAGACGCGCTCAAACTCCGTCTGGT-3′ ...
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bioRxiv - Cancer Biology 2023Quote: KEAP1-sg1 knockout H1299 (4 x 105) cells were transfected with or without plasmid expressing Halo-KEAP1 (Addgene, a gift from Yimon Aye ...
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bioRxiv - Cancer Biology 2023Quote: ... and pRL-TK-int-renilla were purchased from Addgene. SRE-T-Luc ...
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bioRxiv - Cell Biology 2023Quote: ... a pShuttle-U6 vector (Addgene plasmid #13428) was modified by replacing the U6 promoter and multiple cloning site (MCS ...
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bioRxiv - Cell Biology 2023Quote: ... The BioID2 adenoviral vector was generated by cloning BioID2-HA (Addgene plasmid #74224) into the pShuttle-CMV vector ...
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bioRxiv - Cell Biology 2023Quote: ... The FUCCI adenoviral vector was generated by cloning tFucci(CA)5 (Addgene plasmid #153521) into the pShuttle-CMV vector ...
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bioRxiv - Cell Biology 2023Quote: ... and EGFP of pSIL-eGFP (Addgene plasmid #52675) to form a pShuttle-U6-MCS-CMV-GFP vector ...
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bioRxiv - Biophysics 2023Quote: TIA1 proteins were expressed in the BL21DE3 bacterial cell line from the pET28b plasmid (Addgene) containing the Mus musculus (mouse ...
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bioRxiv - Biophysics 2023Quote: ... followed by ligation in place of the mApple sequence in the mApple-CD36-C-10 vector (Addgene, Watertown, MA plasmid # 54874 (5)) ...
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bioRxiv - Biophysics 2023Quote: ... The DNA fragment encoding heteronu-clear ribonucleoprotein A1 (hnRNPA1) was amplified from pET9d-hnRNP-A1 (#23026, Addgene) using PCR and inserted into mCherry-C1 (pmCherry-hnRNPA1) ...
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bioRxiv - Cancer Biology 2023Quote: ... Amplified full-length KEAP1 WT and the HA-Clover construct (Addgene plasmid #163336) were digested with the EcoRV and NotI enzymes (NEB ...
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bioRxiv - Cancer Biology 2023Quote: ... coli cells were transformed with the pET28a-His6-KEAP1 vector (Addgene plasmid #62454). Colonies were grown in LB broth containing 50 μM ZnCl2 and 50 μg/ml kanamycin at 37 °C until cultures reached an OD600 of 0.6-0.8 and then were allowed to cool to room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... PCDH-Flag-c-MYC was a gift from Hening Lin (Addgene plasmid # 102626; http://n2t.net/addgene:102626; RRID: Addgene_102626).
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bioRxiv - Cancer Biology 2023Quote: ... Plasmids for delivery of Cas9 and sgRNAs were generated using BbsI-mediated Golden Gate cloning of annealed single-stranded oligonucleotides into pX330-U6-Chimeric_BB-CBh-hSpCas9 (pX330, Addgene #42230), pSpCas9(BB)-2A-Puro (PX459 ...
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bioRxiv - Cancer Biology 2023Quote: ... pSpCas9(BB)-2A-Puro (PX459) V2.0 (pX459_puro, Addgene #62988), pSpCas9(BB)-2A-Hygro (pX459_hygro ...
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bioRxiv - Cancer Biology 2023Quote: The lentivirus shRNA plasmids targeting mouse RelA and Pkci were generated by cloning annealed oligos into AgeI and EcoRI sites of pLKO.1-hygro-ctrl plasmid (Addgene #24150). Following target sequences and oligos were utilized ...
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bioRxiv - Cancer Biology 2023Quote: ... and empty pKLO.1-hygro (Addgene, 24150) were used as negative controls.
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bioRxiv - Cancer Biology 2023Quote: ... and pMD2.G (Addgene plasmid #12259), a kind gift from Didier Trono ...
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bioRxiv - Cancer Biology 2023Quote: ... according to the manufacturer’s instructions with pSpCas9(BB)-2A-GFP (PX458) plasmid (a gift from Feng Zhang; Addgene plasmid #48138; http://n2t.net/addgene:48138; research resource identifier: Addgene_48138) containing clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9 ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 μg pMD2.G (Addgene) envelope plasmid and 130 μL 1 μg / mL polyethylenimine (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... and lentiGuide-Puro (Addgene plasmid 52963) as previously described [163] ...
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bioRxiv - Cancer Biology 2023Quote: The full length of IRX4_PEP1 was PCR amplified and cloned into the pDONR223 vector (Addgene) with the DYKDDDDK Flag tag by the BP recombination reaction protocol (Gateway® technology ...
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bioRxiv - Cancer Biology 2023Quote: ... pINDUCER21(ORF-EG) (Addgene) by the LR recombination reaction protocol (Gateway® technology (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: HA-EZH2 plasmid mutant constructs were generated using site directed mutagenesis of pCMV-HA-EZH2 a gift from Kristian Helin (Addgene plasmid # 24230 ...
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bioRxiv - Cancer Biology 2023Quote: ... Elements originating from the PB-gRNA-puro plasmid (Addgene #121121; a gift from Pablo Navarro) (PubMed 30846691 ...
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bioRxiv - Cancer Biology 2023Quote: ... (24)] were subcloned into Tet-pLKO-puro vector (Addgene #21915). The lentiviruses encoding shPTPN11 were packaged in HEK293T cells ...
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bioRxiv - Cancer Biology 2023Quote: ... and pEGFP-C3-hYAP1 was a gift from Marius Sudol (addgene plasmid # 17843; RRID: Addgene_17843), addgene (Watertown ...
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bioRxiv - Cancer Biology 2023Quote: pFLAG-YAP1 was a gift from Yutaka Hata (addgene plasmid # 66853; RRID: Addgene_66853) and pEGFP-C3-hYAP1 was a gift from Marius Sudol (addgene plasmid # 17843 ...
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bioRxiv - Cancer Biology 2023Quote: ... along with the envelop and packaging plasmids pCMV-VSV-G (Addgene #8454) (38 ...
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bioRxiv - Cancer Biology 2023Quote: ... Bart Williams with either LV-GFP (Addgene #25999) (39 ...
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bioRxiv - Cancer Biology 2023Quote: ... The ITGB3 sequence (pcDNA3.1-beta-3 was a gift from Timothy Springer; Addgene plasmid # 27289) was subcloned into pLenti6.3/TO/V5-Blasti (A11144 ...
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bioRxiv - Cancer Biology 2023Quote: The sgRNAs specific for 5’ to the region of interest were cloned in pLentiCRISPRv2 (Addgene, #52961). sgRNAs corresponding to 3’ to the region of interest was cloned in pDecko-mCherry (Addgene ...
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bioRxiv - Cancer Biology 2023Quote: ... and psPAX2 (Addgene #12260). NF1-MET cells were infected with 500 μL of 0.45 μm filtered virus with polybrene (EMD Millipore) ...
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bioRxiv - Cancer Biology 2023Quote: ... was obtained from Addgene (item #83469). Gαi2R179C and GαoAR243H mutants were constructed using pcDNA3.1 wild-type human ee-tagged-Gαi2- and ee-tagged-GαoA plasmids as template (cDNA Resource Center ...
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bioRxiv - Cancer Biology 2023Quote: ... and then into the destination vector pLIX_402 (Addgene, item #41394) using the Gateway cloning system.
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bioRxiv - Cancer Biology 2023Quote: AW13516 cell lines stably expressing either non-targeting scrambled shRNA (#1864) (Addgene, Cambridge, Massachusetts, USA) or shRNAs targeting C1QBP or TRIM23 were employed to study the effect of knockdown on the tumorigenic potential of cancer cells ...
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bioRxiv - Cancer Biology 2023Quote: ... wild type TP53 cDNA was amplified from p53-GFP plasmid (Cat #11770) (Addgene, Cambridge, Massachusetts, USA) and cloned into pFN21A (Promega Corporation ...
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bioRxiv - Cancer Biology 2023Quote: ... 101000053) according to the manufacturer’s instructions with pSpCas9(BB)-2A-GFP (PX458) plasmid (a gift from Feng Zhang; Addgene plasmid #48138 ...